• 제목/요약/키워드: 1R5F

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밤나무 잉크병균, Phytophthora katsurae의 검출을 위한 Duplex PCR (A Duplex PCR for Detection of Phytophthora katsurae Causing Chestnut Ink Disease)

  • 이동현;이선근;김혜정;이상현;이상용;이종규
    • 식물병연구
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    • 제18권2호
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    • pp.73-79
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    • 2012
  • Phytophthora katsurae는 밤나무 잉크병의 원인이 되는 병원성 균류이다. P. katsurae를 검출하기 위하여 2개의 duplex PCR primer set을 제작하였다(SOPC 1F/1R+KatI 3F/5R, SOPC 1-1F/1-1R+KatI 3F/5R). SOPC 1F/1R과 SOPC 1-1F/1-1R primer pair는 sequence characteristic amplification regions(SCAR)를 이용하여 제작하였고, KatI 3F/5R primer pair는 internal transcribed spacer(ITS) 부위로부터 제작하였다. 추출한 genomic DNA를 1 mg/ml에서 1 ng/ml까지 10배씩 순차적으로 희석하여 균사량에 따른 Duplex PCR의 민감도를 확인한 결과, 2개의 primer set은 각각 1 ${\mu}g/ml$와 100 ng/ml까지 검출이 가능하였다. 유주포자를 $1{\times}10^6$부터 $1{\times}10^2$ cells/ml까지 10배씩 순차적으로 희석하고 genomic DNA를 추출하여 P. katsurae의 유주포자량에 의한 검출 한계를 확인한 결과, 2개의 primer set 모두 $1{\times}10^5$ cells/ml까지 검출할 수 있었다. 각각의 primer set은 PCR 검출을 실시하였을 때 P. katsurae 균주에서만 PCR 산물이 증폭된 반면에, Phytophthora 16종에서는 P. katsurae에 특이적인 PCR 증폭산물이 생성되지 않았다. 따라서, 2개의 primer set을 이용한 Duplex PCR은 P. katsurae의 신속하고 효과적인 검출에 유용하게 활용될 수 있을 것이다.

환의 PRIME SPECTRUM에 관하여 (ON THE PRIME SPECTRUM OF A RING)

  • 김응태
    • 한국수학교육학회지시리즈A:수학교육
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    • 제12권2호
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    • pp.5-12
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    • 1974
  • 단위원을 가지는 하환환에 있어서의 Prime Spectrum에 관하여 다음 세가지 사실을 증명하였다. 1. X를 환 R의 prime spectrum, C(X)를 X에서 정의되는 실연적함수의 환, X를 C(X)의 maximal spectrum이라 하면 X는 C(X)의 prime spectrum의 부분공간으로서의 한 T-space로 된다. N을 환 R의 nilradical이라 하면, R/N이 regula 이면 X와 X는 위상동형이다. 2. f: R$\longrightarrow$R'을 ring homomorphism, P를 R의 한 Prime ideal, $R_{p}$, R'$_{p}$를 각각 S=R-P 및 f(S)에 관한 분수환(ring of fraction)이라 하고, k(P)를 local ring $R_{p}$의 residue' field라 할 때, R'의 prime spectrum의 부분공간인 $f^{*-1}$(P)는 k(P)(equation omitted)$_{R}$R'의 prime spectrum과 위상동형이다. 단 f*는 f*(Q)=$f^{-1}$(Q)로서 정의되는 함수 s*:Spec(R')$\longrightarrow$Spec(R)이다. 3. X를 환 S의 prime spectrum, N을 R의 nilradical이라 할 때, 다음 네가지 사실은 동치이다. (1) R/N 은 regular 이다. (2) X는 Zarski topology에 관하여 Hausdorff 공간이다. (3) X에서의 Zarski topology와 constructible topology와는 일치한다. (4) R의 임의의 원소 f에 대하여 f를 포함하지 않는 R의 prime ideal 전체의 집합 $X_{f}$는 Zarski topology에 관하여 개집합인 동시에 폐집합이다.폐집합이다....

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남자대학생의 자기 이미지와 의복행동에 관한 연구 (Self-Image and Clothing Behavior of College Men)

  • 이명희;김현주
    • 복식
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    • 제38권
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    • pp.323-336
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    • 1998
  • The purpose of this study was to classify the contents of self-image of college men and to investigate how clothing behavior was influenced by self-image, age, major, and the expenses of clothing. Samples were 342 male students of univer-sity in Seoul. Results of this study were following : 1. Four factors of self-image derived by factor analysis : F. 1‘mature-unmatured’;F. 2‘characteristic-ordinary’; F. 3‘conservative-open’; F. 4‘dominant-submissive’.2. 5 variables of clothing behavior were influenced by real self-image. Fashion was influenced by characteristic, open, dominant image, and the expenses of clothing. The ex-planatory power of the 4 variables was 29.3%. Aesthetics was influenced by characteristic, submissive, conservative image, the expenses of clothing, and age(R2=28.7%). Modesty was influenced by conservative and ordinary im-age(R2=6.5%). Coformity was influenced by ordinary image and the expenses of clothing (R2=5.7%). Comfort was influenced by dominant image and age(R2=5.3%). 3. 5 variables of clothing behavior were influenced by ideal self-image. Fashion was influenced by unmatured, characteristic, sub-missive image, and the expenses of clothing (R2=28.9%). Aesthetics was influenced by characteristic image, the expenses of clothing, and age(R2=23.5%). Modesty was influenced by conservative, mature, and ordinary image (R2=8.3%). Coformity was influenced by ordinary image(R2=1.9%). Comfort was influenced by mature image and age(R2=5.6%). The findings indicated that self-image had a significant effect on fashion and aesthetics, and characteristic image and conservative image were important variables that affected clothing behavior of college men.

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PSN-PMN-PZT 세라믹스의 유전 및 압전 특성과 공진 주파수의 온도안정성 (Dielectric, Piezoelectric Properties and Temperature Stability of Resonant Frequency in PSN-PMN-PZT Ceramics)

  • 윤광희;류주현;민석규;이명수;서성재
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2000년도 하계학술대회 논문집
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    • pp.391-395
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    • 2000
  • In this study, the temperature coefficient of resonant frequency(TC $F_{r}$), dielectric and piezoelectric properties of Pb[(S $b_{1}$2/N $b_{1}$2/)$_{0.0035}$-(M $n_{1}$3/N $b_{2}$3/)$_{0.0065}$-(Z $r_{x}$ $Ti_{1-x}$ )$_{0.90}$] $O_3$ceramics is investigated with Zr/Ti ratio. The dielectric constant and electromechanical coupling factor( $k_{p}$) showed the highest values of 1257, 0.562 respectively when the Zr/Ti ratio is 49.5/50.5. The mechanical quality factor( $Q_{m}$) is the lowest value of 713 when the Zr/Ti ratio is 49.5/50.5, and increased with the decrease of the Zr/Ti ratio. The temperature coefficient of resonant frequency(TC $F_{r}$) change abruptly at the morphotropic phase boundary(MPB), which is between the rhombohedral phase with highly negative TC $F_{r}$ of -106ppm/$^{\circ}C$ and the tetragonal phase with highly positive TC $F_{r}$ of +64pp $m^{\circ}C$ as Zr/Ti ratio changes from 50/50 to 49.5/50.5.50.5..5.50.5.5.

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PSN-PMN-PZT 세라믹스의 Zr/Ti 비에 따른 전기적 특성과 공진주파수의 온도안정성 (Electrical Properties and Temperature Stability of resonant Frequency with Zr/Ti ratio in PSN-PMN-PZT Ceramics)

  • 류주현;윤광희;민석규;이명수;서성재
    • 한국전기전자재료학회논문지
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    • 제13권8호
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    • pp.675-680
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    • 2000
  • In this study the temperature coefficient of resonant frequency(TC $F_{r}$) dielectric and piezoelectric properties of Pb[(S $b_{1}$2//N $b_{1}$2/)$_{0.065}$)-(Z $r_{x}$, $Ti_{1-x}$ )$_{0.90}$] $O_3$ceramics were investigated with Zr/Ti ratio. The compositions near the morphotropic phase boundary (MPB) appeared when Zr/Ti ratio was 49.5/50.5 The dielectric constant and electromechanical coupling factor( $k_{p}$) also showed the highest values of 1,257, 0.653 respectively when the Zr/Ti ratio was 49.5/50.5 Moreover the mechanical quality factor( $Q_{m}$) showed th lowest value of 713 when the Zr/Ti ratio. The temperature coefficient of resonant frequency(TC $F_{r}$) abruptly change at the morphotropic phase boundary(MPB) which existed between the rhombohedral phase with highly negative TC $F_{r}$ of -106ppm/$^{\circ}C$ and the tetragonal phase with highly positive TC $F_{r}$ of +64pp $m^{\circ}C$ as Zr/Ti ratio varied from 50/50 to 49.5/50.5.50.5..50.5.

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살충성(殺蟲性) O,O-Diethylphenylphosphate유도체(誘導體)들에 의(依)한 Acetylcholinesterase의 Phosphorylation에 미치는 자유(自由)에너지 관계(關係) (Linear Free Energy Relationship on the Phosphorylation of Acetylcholinesterase by Insecticidal O,O-Diethylphenylphosphate Derivatives)

  • 성낙도
    • 농업과학연구
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    • 제11권1호
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    • pp.176-181
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    • 1984
  • 살충성 O,O-diethylphenylphosphate (A)와 3,5-dimethylphenyl-O,O-diethylphosphate 유도체 (B)에 의한 acetylcholinesterase(Ach E)의 phosphorylation 반응에 미치는 정량적인 자유에너지 관계(LFER)를 알아보기 위하여 Ach E의 두 작용점인 esteratic-anionic site에 대응하는 (A) 및 (B)의 P원자 및 meta 위치간의 공간직선 거리 및 알짜전하를 확장 H$\ddot{u}$ckel이론(EHT)에 의한 MO 계산 방법으로 계산하고 여러 가지 치환기상수와 $pI_{50}$ 상수를 Hammett 및 Okamoto-Brown식, Taft와 Swaln-Lupton의 DSP식에 적용한 회귀분석 결과로부터 살충작용에 미치는 정량적인 자유에너지 관계, %R과 %F를 계산하여 검토하였다. 즉, (A) 및 (B)의 살충효과는 서로 비슷하였으며 blending coefficient (${\lambda}$)는 모두 ${\lambda}$<1의 값을 나타내었다. (A) 및 (B)의 resonance effect (%R)와 field effect (%F)는 각각 %R=33.5, %F=66.5 그리고 %R=2, %F=98로 각각 계산되었는데(B)와 같이 phenyl group에 bulky한 소수성의 methyl group이 결합될수록 resonance effect($R_{\pi}$)보다는 direct through space field effect ($F_D$)가 아닌 through space field effect가 더 크게 영향을 미치게 된다는 사실을 알 수 있었다.

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RIN-m5F 세포에서 야관청혈탕(夜關淸血湯)이 인슐린 분비에 미치는 영향 (Effect of YCT on Insulin Secretion in RIN-m5F Cells)

  • 김진미;조충식;김철중
    • 대한한의학회지
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    • 제31권4호
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    • pp.20-37
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    • 2010
  • Objective: This study was performed to investigate the effect of Yagwan-cheunghyeoltang (YCT) on insulin secretion in RIN-m5F cells. Methods: After treatment with various concentrations of YCT to RIN-m5F cells, cell viability, free radical-scavenging activity, SOD activity, and insulin secretion were measured. Additionally, insulin-related gene expressions were measured using real-time RT-PCR. Results: 1. YCT didn't show any influence on RIN-m5F cells viability. 2. YCT showed free radical-scavenging activity by 16% at $100{\mu}g/m{\ell}$ of concentration. 3. YCT showed enhancement of SOD activity by 60% at $100{\mu}g/m{\ell}$ of concentration. 4. YCT significantly increased insulin secretion in RIN-m5F cells in a dose-dependent manner. 5. YCT up-regulated INS-1, INS-2, IRS-1, IRS-2 and IRS-3 mRNA expressions compared to the control group. 6. YCT down-regulated INS-R, GCK, GLP-1R and GLP-2R mRNA expressions compared to the control group. Conclusion: YCT has pharmaceutical properties enhancing insulin production and controlling glucose-associated metabolism, and could be a candidate for drug development after further research.

RADIUS CONSTANTS FOR FUNCTIONS ASSOCIATED WITH A LIMACON DOMAIN

  • Cho, Nak Eun;Swaminathan, Anbhu;Wani, Lateef Ahmad
    • 대한수학회지
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    • 제59권2호
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    • pp.353-365
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    • 2022
  • Let 𝓐 be the collection of analytic functions f defined in 𝔻 := {ξ ∈ ℂ : |ξ| < 1} such that f(0) = f'(0) - 1 = 0. Using the concept of subordination (≺), we define $$S^*_{\ell}\;:=\;\{f{\in}A:\;\frac{{\xi}f^{\prime}({\xi})}{f({\xi})}{\prec}{\Phi}_{\ell}(\xi)=1+{\sqrt{2}{\xi}}+{\frac{{\xi}^2}{2}},\;{\xi}{\in}{\mathbb{D}}\}$$, where the function 𝚽(ξ) maps 𝔻 univalently onto the region Ω bounded by the limacon curve (9u2 + 9v2 - 18u + 5)2 - 16(9u2 + 9v2 - 6u + 1) = 0. For 0 < r < 1, let 𝔻r := {ξ ∈ ℂ : |ξ| < r} and 𝒢 be some geometrically defined subfamily of 𝓐. In this paper, we find the largest number 𝜌 ∈ (0, 1) and some function f0 ∈ 𝒢 such that for each f ∈ 𝒢 𝓛f (𝔻r) ⊂ Ω for every 0 < r ≤ 𝜌, and $${\mathcal{L} _{f_0}}({\partial}{\mathbb{D}_{\rho})\;{\cap}\;{\partial}{\Omega}_{\ell}\;{\not=}\;{\emptyset}$$, where the function 𝓛f : 𝔻 → ℂ is given by $${\mathcal{L}}_f({\xi})\;:=\;{\frac{{\xi}f^{\prime}(\xi)}{f(\xi)}},\;f{\in}{\mathcal{A}}$$. Moreover, certain graphical illustrations are provided in support of the results discussed in this paper.

소방공무원의 직무질환에 관한 연구 (A Study on Occupational Diseases of Fire Officials)

  • 조광래
    • 시큐리티연구
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    • 제61호
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    • pp.109-135
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    • 2019
  • 본 연구는 소방공무원의 직무질환(진료건수)에 대한 시계열 분석 등 기술적 탐색 연구를 진행하는데 그 목적이 있다. 구체적인 연구결과는 다음과 같다. 첫째, 소방공무원의 직무질환에 대한 평균추이는 ① 내과 질병은 12월이 9.24%로 가장 높고, 2월이 7.76%로 가장 낮고, ② 이비인후과 질병은 12월이 9.29%로 가장 높고, 8월이 6.74%로 가장 낮으며, ③ 피부과 질병은 7월이 10.03%로 가장 높고, 1월과 2월이 7.35%로 가장 낮고, ④ 외과 질병은 11월이 10.38%로 가장 높고, 2월이 5.62%로 가장 낮으며, ⑤ 정형외과 질병은 3월이 9.69%로 가장 높고, 11월이 7.52%로 가장 낮고, ⑥ 신경외과 질병은 4월이 9.33%로 가장 높고, 2월이 6.82%로 가장 낮으며, ⑦ 재활의학과 질병은 12월이 9.47%로 가장 높고, 10월이 7.06%로 가장 낮고, ⑧ 정신건강의학과 질병은 12월이 9.93%로 가장 높고, 5월이 6.51%로 가장 낮은 것으로 나타났다. 둘째, 소방공무원의 직무질환에 대한 계절분해는 ① 내과 질병은 1.075(S) × 189.355(T·C) × 1.174(R) = 238.975(F), ② 이비인후과 질병은 1.023(S) × 69.605(T·C) × 1.040(R) = 74.000(F), ③ 피부과 질병은 1.002(S) × 73.088(T·C) × 0.874(R) = 64.000(F), ④ 외과 질병은 1.099(S) × 27.229(T·C) × 0.669(R) = 20.000(F), ⑤ 정형외과 질병은 1.115(S) × 73.182(T·C) × 1.213(R) = 99.000(F), ⑥ 신경외과 질병은 0.993(S) × 27.836(T·C) × 1.303(R) = 36.000(F), ⑦ 재활의학과 질병은 1.029(S) × 62.417(T·C) × 1.152(R) = 74.000(F), ⑧ 정신건강의학과 질병은 1.210(S) × 8.781(T·C) × 1.035(R) = 11.000(F) 등으로 나타났다.

Quantitative Determination of the Triterpenoids and Total Tannin in Korean Rubus species by HPLC

  • Kim, Min-Young;Tapondjou, Leon Azefack;Park, Hee-Juhn
    • Natural Product Sciences
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    • 제20권4호
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    • pp.290-295
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    • 2014
  • The triterpenoids contained in four Rubus species (Rosaceae) were quantitatively analyzed using HPLC to select plants with large quantities of niga-ichigoside $F_1$. Unripe fruits, ripe fruits, and leaves were extracted to estimate the quantity of niga-ichigoside $F_1$, together with Rubus-specific $19{\alpha}$-hydroxyursane-type triterpenoids, euscaphic acid, tormentic acid, and kaji-ichigoside $F_1$, and a dimeric triterpenoid coreanoside $F_1$. Niga-ichigoside $F_1$ was most abundant in the leaves of R. crataegifolius (23.4 mg/g dry weight). The amount of triterpenoid in the black, ripe fruits of R. coreanus was lower than the unripe fruits of the same plant. On the other hand, the ripe fruits of three plants, R. crataegifolius, R. parvifolius and R. pungens var. oldhami, which are reddish, contained higher or similar level of triterpenoids than their unripe fruits. In addition, the concentration of niga-ichigoside $F_1$ in the ripe fruit of R. crataegifolius was 20.5 mg/g, suggesting that the fruits could be used as a functional food. Methyl gallate and ellagic acid were used as quantitative indices of total tannin. Methyl gallate levels were higher in ripe fruits than unripe fruits in R. crataegifolius, R. pungens var. oldhami, and R. parvifolius. In R. crataegifolius, the quantity of methyl gallate was 30.5 mg/g in ripe fruit, but 1.19 mg/g in unripe fruit.