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Isolation and Structure Identification of Antibacterial Substances from the Rhizome of Zingiber mioga Roscoe (양하의 근경에서 항균성 물질 분리 및 구조동정)

  • Kim, Seong-Cheol;Song, Eun-Young;Kim, Kong-Ho;Kwon, Hyeog-Mo;Kang, Sang-Heon;Park, Ki-Hun;Jung, Yong-Hwan;Jang, Ki-Chang
    • Applied Biological Chemistry
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    • v.46 no.3
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    • pp.246-250
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    • 2003
  • In order to isolate antibacterial substances from the rhizome of Zingiber mioga Roscoe, the ethanol extracts was fractionated according to the activity against Bacillus subtilis, B. cereus and Staphylococcus aureus. Three antibacterial substances were isolated and purified by column chromatography and recrystallization. Compounds I and III showed activity against all the tested bacterias and compound II exhibited the activity against B. subtilis and B. cereus S. aureus. Compound I was examined antimicrobial activity against B. subtilis, B. cereus and S. aureus by optical density using Bioscreen C. Compound I showed strong growth inhibition at 10 ppm on B. subtilis and B. cereus for 72 hrs, and at 25 ppm on S. aureus. On the basis of spectrometric studies including $1^H-NMR$, ${13}^C-NMR$, DEPT, IH-lH COSY, HMQC, HMBC and IR, compounds I, II and III were identified as $(E)-8{\beta}(17)-epoxylabd-12-ene-15,16-dial\;(C_{20}H_{30}O_3,\;MW=318)$, galanolactone $(C_{20}H_{30}O_3\;MW=318)$ and galanal A $(C_{20}H_{30}O_3,\;MW=318)$, respectively. These results are the first reports on the isolation of $(E)-8{\beta}(17)-epoxylabd-12-ene-15,16-dial, galanolactone and galanal A from the rhizome of Zingiber mioga.

Taxonomic characterization of novel Hymenobacter sp. B2 isolated from a freshwater environment (민물환경에서 분리된 novel Hymenobacter sp. B2의 분류학적 특성연구)

  • Young-Min Bae
    • Journal of the Korean Applied Science and Technology
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    • v.40 no.4
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    • pp.881-889
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    • 2023
  • The genus Hymenobacter, type genus of the family Hymenobacteraceae and a member of the phylum Bacteroidota includes gram-negative and red-pigmented rods. Those bacteria have been isolated from various environments of the earth. I isolated a red-pigmented, gram-negative rod from a pond in the campus of the Changwon University, Changwon, Kyeongnam and designated this bacterium as strain B2. Strain B2 was further analyzed phylogenetically and biochemically, and concluded as a member of genus Hymenobacter. BLAST search of the 16S rRNA gene sequence of strain B2 showed its homology lower than 98.7% with those sequences of the other bacteria whose 16S rRNA gene sequences have been reported. Fatty acid composition of the strain B2 was analyzed and its major fatty acids are summed feature 3(C16:1 ω7c and/or C16:1 ω6c, 22.8%), iso-C15:0 (16.2%), anteiso-C15:0(12.9%), C16:1ω5c(12.4%) and summed feature 4 (iso-C17:1 I/anteiso-C17:1)(9.5%) showing significant differences in fatty acid compositions between strain B2 and the other known Hymenobacter species. DNA sequence of 16S rRNA gene of strain B2 was deposited in genbank under accession number OQ318247.

Fabrication of a Miniaturized 5.8 GHz filter using a GaAs Substrate (GaAs 기판을 이용한 5.8 GHz 소형 필터제작)

  • Song, Ki-Young;Kim, Kyoung-Taek;Jung, Sung-Hae
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.17 no.6
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    • pp.598-601
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    • 2004
  • A microstrip filter has been designed and fabricated in hairpin form on a GaAs substrate and its characteristics such as the insertion loss, return loss, and bandwidth have been investigated. The presented filter is realized with relatively small size, 4.5 by 3.6 mm. The insertion loss and return loss of the filter are 1.17 dB and 30 dB, respectively. The filter exhibits 3 dB-bandwidth of 350 MHz at the center frequency, 5.890 GHz. The experimental results show good agreement with the numerical ones. However, the measured center frequency is about 100 MHz higher than the designed one.

Effects of N-and C-Substituents on Protonation of 14-Membered Tetraaza Macrocycles and Formation of their Copper(II) and Nickel(II) Complexes

  • Shin-Geol Kang;Mi-Seon Kim;Jang-Sik Choi;Moon Hwan Cho
    • Bulletin of the Korean Chemical Society
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    • v.14 no.5
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    • pp.594-598
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    • 1993
  • The protonation constants of the 14-membered tetraaza macrocycles A(3,14-dimethyl-2,6,13,17-tetraazatricyclo$[l6.4.0^{1,18}.0^{7,12}]$docosane) and B(2,3,6,13,14,17-hexamethyl-2,6,13,17-tetraazatric yclo-[l6.4.$0^{1,18}.0^{7,12}$]docosane) were measured by potentiometry. The formation constants of each of these ligands with copper(II) and nickel(II) were determined by an out-of-cell spectrophotometric method. The results indicate that the per-N-methylated macrocycle B exhibits much higher selectivity for complex formation with copper(II) over nickel(II) ion than A and other related 14-membered tetraaza macrocycles. The effects of the N-and C-substituents on the basicity and the metal ion selectivity of the ligands are discussed. The synthesis and properties of copper(II) and nickel(II) complexes of B are also described.

Serially Concatenated Neural Linear Transversal Equalizer/Turbo Code Detection for High Density Nonlinear Magnetic Storage Channels (고밀도 비선형 자기 저장 채널을 위한 신경망 등화기와 터보 코드의 연접 데이터 복호 방법)

  • Lee, Jun;Lee, Jae-Jin
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.25 no.11B
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    • pp.1879-1883
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    • 2000
  • 디지털 자기 기록 저장기기에서 채널 밀도가 증가하면 심각한 인접심볼 간섭과 비선형 왜곡이 야기된다. 본 논문에서는 심각한 비선형 인접심볼 간섭과 비선형 왜곡을 극복하기 위한 방법으로 기존의 등화기 대신 NLTE(neural linear transversal equalizer)를 등화기로 사용하고 검출기로는 터보 코드를 사용한 NLTE/TC 구조를 제안한다. 채널 밀도 S=2.5에서 부분 삭제가 0.7 정도 존재할 때, 코드율이 8/9일 때는 $10^{-5}$의 비트 에러율을 18dB 이후에서 만족하며, 코드율이 16/17일 때는 20dB 이후에서 만족함을 알 수 있었다. 채널 밀도 S=3에서 부분 삭제가 0.6 정도 존재할 때 코드율이 8/9일 때는 $10^{-5}$의 비트 에러율을 22dB 이후에서 만족하고, 코드율이 16/17일 때는 24dB 이후에서 만족함을 확인할 수 있었다.

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Isolation of Bacillus amyloliquefaciens Strains with Antifungal Activities from Meju

  • Lee, Hwang-A;Kim, Jeong-Hwan
    • Preventive Nutrition and Food Science
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    • v.17 no.1
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    • pp.64-70
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    • 2012
  • Bacilli with fibrinolytic activities were isolated from traditionally-prepared Meju and some of these strains showed strong antifungal activities. One isolate, MJ1-4, showed the strongest antifungal activity. MJ1-4 and other isolates were identified as B. amyloliquefaciens strains by recA gene sequencing and RAPD-PCR results. B. amyloliqufaciens MJ1-4 efficiently inhibited an Aspergillus spp.-producing aflatoxin B1 ($AFB_1$) and a Penicillium spp.-producing ochratoxin (OTA) in addition to other fungi. Antifungal activity of B. amyloliquefaciens MJ1-4 culture reached its maximum (40 AU/mg protein) in LB or TSB medium around 48 hr at $37^{\circ}C$. Antifungal activity of the concentrated culture supernatant was not decreased significantly by protease treatments, implying that the antifungal substance might not be a simple peptide or protein. Considering its antifungal and fibrinolytic activities together, B. amyloliquefaciens MJ1-4 can serve as a starter for fermented soyfoods such as Cheonggukjang and Doenjang.

Short Form Versions of MacArthur-Bates Communicative Development Inventories - Korean (M-B CDI-K) (M-B CDI - Korean 축약판 개발)

  • Pae, So-Yeong;Kwak, Keum-Joo;Kim, Mi-Bae;Lee, Hyun-Suk;Jung, Kyung-Hee
    • Speech Sciences
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    • v.15 no.2
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    • pp.119-129
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    • 2008
  • The purpose of this study were to develop short form versions of MacArthur-Bates Communicative Development Inventories - Korean and to discuss clinical implications. Two short versions were developed: one for $9\sim17$ month-olds(including 69 words and 17 object manipulation skills) and the other for $18\sim35$ month-olds (including 128 words and 5 grammatical items). Short versions seemed to be representative of full versions and showed developmental validity. Short versions showed concomitant validity with SELSI which is a standardized test for Korean children under 37 month-olds. Norm tables for words and criteria for object manipulation and grammaticality are also provided. M-B CDI-K short versions could be used as a preliminary screening tool to identify Korean children with language impairment economically and efficiently. Cautions using short versions of M-B CDI-K are discussed.

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An study on the Effects of Visual and Aural Information on Environmental Sound Amenity Evaluation (시각 및 청각 정보가 환경음의 쾌적성 평가에 미치는 영향에 관한 연구)

  • Shin, Hoon;Baek, Kun-Jong;Song, Min-Jeong;Jang, Gil-Soo
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.17 no.9
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    • pp.813-818
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    • 2007
  • This study aims to know the effect of road traffic noise perception when the visual and aural information is added in a laboratory experiment. ME (magnitude estimation) and SD (semantic differential method) evaluation on the effect of visual and aural effect were carried out by 43 university students. As the result, up to 10 % of psychological reduction effect was shown under the 65 dB(A). As the noise level, it was analyzed that the vision affected about 7 dB(A) and sound affected 5 dB(A). However, if these two are given simultaneously, mainly sound affects to reduce the annoyance of noise and the vision next. Compared with the urban central circumstances, this effect (2 dB(A) under 65 dB(A) noise) was shown smaller than field test.

A study on Noise Generation and Awareness of the Fire Alarm Sound in the Factory (공장소음으로 인한 화재 경보음 인지 영향정도에 관한 연구)

  • Lee, Young-Sam
    • Journal of the Korea Safety Management & Science
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    • v.17 no.4
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    • pp.97-103
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    • 2015
  • The people who are in the noise factory are difficult to hear and recognize about fire and to evacuate quickly when a fire occurs. This study was conducted to analyze workplace's background noise of 31 factories in Incheon and to know the workplace noise level comparing to fire alarm sound level. The measured average noise level was 96.8dB(A). Noise level of measured result was exceeded as 6.8dB(A) comparing to NFSC (National Fire Safety Code) noise level of 90dB. NFPA suggests that when background noise exceeds 85dB or more than that, other methods should be considered to know alarm sound. Also fire alarm sound should exceed more than 15dB(A) comparing to background noise level. Therefore it was concluded that the design of the fire alarm systems should not be applied to the workplace without considering background noise and it needs to be improved with regulations and the systems.

The Interaction of Mastoparan B from Venom of a Hornet Vespa Basalis with Phospholipid Matrices

  • 박남규;Yuhji Yamato;Sannamu Lee;Gohsuke Sugihara;박장수;강신원
    • Bulletin of the Korean Chemical Society
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    • v.17 no.3
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    • pp.239-244
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    • 1996
  • Mastoparan B (MP-B) that is a novel MP isolated from the hornet Vespa basalis, was studied as compared with MP, in terms of interaction with phospholipid bilayer and antimicrobial activity. MP-B has more hydrophilic amino acid residues in hydrophilic face of amphiphilic α-helical structure than MP. The both peptides exhibited considerably different effect on interaction with lipid bilayers, e.g. their conformation in the presence of acidic and neutral liposomes, dye-release ability from encapsulated liposomes, but on the whole the interaction mode was similar. On antimicrobial activity, MP had a strong activity against Gram-positive bacteria but no against Gram negative ones. Contrary to this, MP-B had a strong activity against Gram-positive and potent against Gram-negative ones. Since both peptides have almost same residues on the hydrophobic side, such more hydrophilic surface on the molecule seems to lead to the subtle change in its interaction with membranes, resulting in the alternation in its biological activity.