• Journal of Microbiology and Biotechnology
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• 제34권6호
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• pp.1328-1339
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• 2024

Cadmium (Cd) is a prevalent environmental contaminant that poses a potential hazard to the health of both humans and animals. In this study, biosynthesized selenium-enriched Lactobacillus plantarum and selenium nanoparticles (SeNPs) were developed and evaluated for their protective effects against Cd-induced hepatic injury in mice through oral administration for 4 weeks. Cadmium exposure resulted in severe impairment of liver function, as evidenced by increased levels of serum markers of liver injury and, oxidative stress and significant damage to liver tissue, and a notable decrease in the diversity of the intestinal microbiota. Oral administration of Se-enriched L. plantarum (LS) reduced cadmium accumulation in the liver by 49.5% and, restored other cadmium-induced damage markers to normal levels. A comparison of the effects with those of L. plantarum (L) and SeNPs isolated from LS revealed that LS could more effectively alleviate hepatic oxidative stress and reduce the intrahepatic inflammatory responses of the liver, further protecting against cadmium-induced liver injury. These findings suggest that the development of LS may be effective at protecting the liver and intestinal tract from cadmium-induced damage.

• 한국정밀공학회지
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• 제29권12호
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• pp.1331-1339
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• 2012

Safety valve used in LNG/LNG-FPSO ships is a high value valve, and it plays an important role in maintaining a fixed level of pressure by emitting LNG gas out of pipes in LNG piping system under the cryogenic and high-pressure condition when the pressure of the system connected with the LNG storage tank and pipes reaches over the set pressure. The structural stability is required for the inner pressure and thermal load because of the cryogenic and high-pressure condition, and a reliability of the safety valve is necessary for impact and deformation by opening the valve. But, the safety valve, which plays a key role for a safety of the transport and storage system, is depended on imports for over 90%, and in domestic production, the design of the valve is performed on the basis of experiences of the works without quantitative analysis for the inner operation characteristics and structural stability of the valve. In this study, impact velocity is calculated by theoretical analysis for obtaining the structural stability of the guide according to the impact load by opening the valve. The shape of the guide and the diaphragm for satisfying the structural stability are suggested and verified by using a thermal-structural analysis.

• Asian-Australasian Journal of Animal Sciences
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• 제30권9호
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• pp.1332-1339
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• 2017

Objective: This study investigated the effects of Bacillus subtilis CSL2 (B. subtilis CSL2) administration before Salmonella challenge on the fecal microbiota and microbial functionality of Hy-line Brown (HLB) laying hens. Methods: Fecal samples were collected from control (CON), Salmonella-infected (SAL) and Salmonella-infected, probiotic-treated (PRO) groups before and after Salmonella challenge for microbiome analysis using 16S rRNA gene pyrosequencing. Results: Infection with Salmonella led to decreased microbial diversity in hen feces; diversity was recovered with Bacillus administration. In addition, Salmonella infection triggered significant alterations in the composition of the fecal microbiota. The abundance of the phylum Firmicutes decreased while that of Proteobacteria, which includes a wide variety of pathogens, increased significantly. Bacillus administration resulted in normal levels of abundance of Firmicutes and Proteobacteria. Analysis of bacterial genera showed that Salmonella challenge decreased the population of Lactobacillus, the most abundant genus, and increased populations of Pseudomonas and Flavobacterium genera by a factor of 3 to 5. On the other hand, Bacillus administration caused the abundance of the Lactobacillus genus to recover to control levels and decreased the population of Pseudomonas significantly. Further analysis of operational taxonomic units revealed a high abundance of genes associated with two-component systems and secretion systems in the SAL group, whereas the PRO group had more genes associated with ribosomes. Conclusion: The results of this study indicate that B. subtilis CSL2 administration can modulate the microbiota in HLB laying hens, potentially acting as a probiotic to protect against Salmonella Gallinarum infection.

• BMB Reports
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• 제29권3호
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• pp.253-260
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• 1996

An enzyme that hydrolyzes flavin-adenine dinucleotide (FAD) was found to be present in rat liver lysosomal membrane prepared from Triton WR-1339 filled lysosomes (tritosomes) purified by flotation on sucrose. This FAD phosphohydrolase (FADase) exhibited optimal activity at pH 8.5 and had an apparent Km of approximately 3.3 mM. The activity was decreased 50~70% by dialysis against EDTA and this was restored by $Zn^{2+}$, $Mg^{+2}$, $Hg^{+2}$, and $Ca^{+2}$ ions inhibited the enzyme, but $F^-$ and molybdate had no effect. The enzyme was also inhibited by p-chloromercuribenzoate (pCMB), reduced glutathione and other thiols, cyanide, and ascorbate. The presence of ATP, ADP, AMP. ${\alpha}-{\beta}-methylene$ ATP, AMP-p-nitrophenyl phosphate (PNP), GMP, and coenzyme A (CoA) decreased the activity on FAD, but pyrimidine nucleotides, adenosine, adenine, or $NAD^+$ were without effect. Phosphate stimulated the activity slightly. FAD phosphohydrolase activity was separated from ATPase and inorganic pyrophosphatase activities by solubilization with detergents and polyacrylamide gel electrophoresis and by linear sucrose density gradient centrifugation suggesting that the enzyme is different from ATPase, inorganic pyrophosphatase, and soluble lysosomal FAD pyrophosphatase. Paper chromatography showed that FAD was hydrolyzed to flavin mononucleotide (FMN) and AMP which were further hydrolyzed to riboflavin and AMP by phosphatases known to be present in lysosomal membranes. Incubation of the intact Iysosomes with pronase showed that the active site of FAD phosphohydrolase must be oriented to the cytosol. The FAD hydrolyzing activity was detected in Golgi, microsome, and plasma membrane, but not in mitochondria or soluble lysosomal preparations.

• 한국멀티미디어학회논문지
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• 제7권10호
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• pp.1339-1353
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• 2004

HTML의 table 태그는 연관된 정보를 기술하기 위한 테이블은 물론이고 웹 문서의 레이아웃을 표현하기 위하여 사용된다. 본 논문에서 는 웹으로부터 유용한 정보를 추출하기 위한 목적의 일환으로 HTML 문서로부터 테이블을 식별하는 효율적인 방법을 제안한다. 제안된 방법은 전처리와 속성-값 연관관계 추출의 두 단계로 구성된다. 전처리 단계에서는 진짜 테이블 또는 레이아웃용으로 사용된 table 태그의 일반적인 특징을 반영한 규칙을 적용하여 진짜 또는 가짜로 명확히 식별이 가능한 table태그를 추출한다. 속성-값 연관관계 추출 단계에서는 테이블 영역을 속성 및 값 영역으로 구분한 후, 값 영역에 대하여 구문적 일관성 검사를 수행한다. 또한 값 영역의 크기가 작아서 구문적 일관성 검사를 수행할 수 없는 경우, 속성-값 영역의 의미적 일관성을 검사한다. 제안된 방법의 성능을 평가하기 위하여 1,393개의 HTML문서로부터 추출한 11,477개의 table 태그를 대상으로 실험한 결과, 평균적으로 97.54%의 정확률과 99.22%의 재현률을 보여 기존 연구보다 우수하였다.

• 대한한의학회지
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• 제27권4호
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• pp.96-104
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• 2006

Insamsansa-eum (Renshenshanzha-yin, ISE) is a new medicine developed to prevent and treat atherosclerotic diseases. To explore antiatherosclerotic effects of ISE, we evaluated the effects of ISE on serum lipids of hypercholesterolemic rats in vivo, as well as its antioxidant activities in vitro. In vitro, ISE showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide radical scavenging activities, and inhibited hemolysis induced by 2,2' -azobis-2-amidinopropane dihydrochloride (AAPH) in a dose-dependent manner. In vivo, ISE significantly inhibited increase of total cholesterol (TC), low-density lipoprotein cholesterol (LDL) and triglyceride (TG) values in both high cholesterol diet and Triton WR-1339 models. It also significantly inhibited decrease of high-density lipoprotein cholesterol (HDL) value in the high cholesterol diet model. Collectively, our data suggest that ISE has the potential to control the risk of atherosclerosis development.

• 대한환경공학회지
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• 제27권12호
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• pp.1332-1339
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• 2005

본 연구의 목적은 310종 다성분 동시분석법을 이용하여 영산강유역 생활하수처리장 방류수에서 유기오염물질의 분포특성을 연구하는 것이다. 그 결과, 5개 시료채취지점에서 많은 유기오염물질이 검출되었고 그 중 농약류, CH구조의 벤젠류와 다환방향족화합물 그리고 CHO구조의 페놀류와 프탈레이트류 화합물이 주요오염물질로서 검출되었다. 특히, 14종의 농약류가 주로 여름철에 방류수에서 검출되었다. 1-Chlorobenzen과 p-octylphenol은 각각 $0.52{\sim}0.61\;{\mu}g/L$과 $0.04{\sim}0.89\;{\mu}g/L$ 농도범위를 가지고 빈번히 검출되는 오염물질이다. 또한, 5개 시료채취지점에서 diethylphthalate를 포함한 18종의 내분비계교란물질이 관찰되었다. 따라서 이러한 연구결과로부터 우리는 영산강유역 생활하수처리장 방류수에서 농약류와 CH, CHO구조를 가진 화학물질들에 대해 특별 관리가 이루어져야 한다고 생각한다.

• 한국컴퓨터산업학회논문지
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• 제2권10호
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• pp.1339-1348
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• 2001

전통적인 상거래 방식에서의 결제시스템으로는 신용장에 의한 방식, 추심에 의한 방식, 송금에 의한 방식 등이 있으며, 신용장방식은 수출자가 대금지급에 대한 확실성과 금융상의 편리, 신용위험의 감소로 보편적으로 가장 많이 선호하고 있다. 추심 방식은 오랜 신용관계에 있는 거래선간에 조심스럽게 이용되고 있다. 송금방식은 소액이나 견본 등의 대금지불에 알맞은 방식이다. 인터넷 전자상거래 방식에서의 결제시스템으로는 지불 브로크형 시스템, 전자화폐시스템, 소액전자지불시스템, 전자자금이체시스템 등이 있다. 본 논문에서는 전통적인 방식의 결제방식과 인터넷을 이용한 결제시스템의 차이와 장ㆍ단점을 비교하였다. 인터넷 결제시스템은 전통적인 방식보다 결제시간을 줄일 수 있는 반면, 소매거래 (B to C)환경에 국한되는 단점을 가지고 있다. 도매거래(B to B)하에서는 전통적인 결제시스템이 안정성, 안전성, 신뢰성 측면에서 인터넷 결제시스템보다 아직까지는 월등한 상황이다.

• Journal of Korean Neurosurgical Society
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• 제29권10호
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• pp.1333-1339
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• 2000

Objective : The goal of this study is to identify the significant radiologic abnormalities in patients complaining headache and to determine predictive factors for clinically significant radiological abnormalities. Method : The study population was 410 patients having underwent CT or MRI study among 1000 patients complaining headache in outpatient basis between 1996-1999. All of these patients answered self-administered questionaire about their headaches. We reviewed the patient's charts and the questionaires and examined the radiologic study results. Result : Of the 410 patients referred for CT or MRI study, male : female ratio was 1 : 1.97. Twenty-five patients(6.1%) revealed clinically significant organic lesions. Mean age was 46.1 in radiologic abnormal group and 48.4 in normal group. Short symptom duration(p<0.01), motor weakness(p<0.05), vomiting(p<0.05), cranial nerve palsy(p<0.05), and trauma history(p<0.05) were factors indicated higher incidence of radiologic abnormality. But, patients age, and severity of headache were not associated with clinically significant radiologic lesion. The ratio of radiologic abnormality was 0.8% in patients not having any risk factor. Conclusion : The results indicate that radiologic study should be done in headache patients having the risk factors such as short symptom duration, motor weakness, vomiting, cranial nerve palsy, trauma history. For patients without any such a risk factor, the radiologic study doesn't seem mandatory.

• 한국산학기술학회논문지
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• 제15권11호
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• pp.6915-6921
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• 2014

본 연구는 미스 반 데어 로에의 일리노이 공과대학 캠퍼스(1339-1359)의 구성과 공간적 특성을 분석하는 것을 주제로 한다. 미스의 건축에 대한 연구가 "보편적 공간"이나 구조적 합리성 등 단일 건축물에 치중되어 왔다면 IIT 캠퍼스는 여러 동의 건물로 공공 공간을 구성한 단지계획이라는 점이 연구의 동기라고 할 수 있다. 본 연구에서는 미스의 기존 작품들과의 연관성을 도출하고 IIT 캠퍼스의 계획의도를 분석하기 위해 미스가 그린 스케치, 배치도, 투시도 등을 분석하였다. 그리하여 도출한 결론은, 첫째 미스는 단순한 형태의 선형건물을 공공 공간을 규정하는 벽처럼 사용하여 "흐르는 공간"을 캠퍼스 전반에 구현하였다. 둘째, IIT 캠퍼스는 그 자체로 도시 공간의 한 일부분으로서 통합되는 "중립적 프레임"이 되었다. 셋째, 캠퍼스의 내부에는 학생들을 위해 외부의 혼잡함으로부터 보호받으며 조용한 안정감을 주는 동시에 주변 공간과 연속되고 개방된 공공 공간을 만들었다.