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Mycelial Production and Amylase Activity of Fungi for Brewing in Different Submerged Culture Conditions (액체배양에서의 양조용 곰팡이의 균체 생산 및 전분분해효소 활성)

  • Noh, Jong-Min;Choi, Ji-Ho;Jung, Seok-Tae;Yeo, Soo-Hwan;Park, Jang-Woo;Lee, Jin-Won;Choi, Han-Seok
    • Journal of the East Asian Society of Dietary Life
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    • v.23 no.6
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    • pp.833-838
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    • 2013
  • In this study, twelve strains of brewing fungi were individually cultivated on wheat extract broth (WEB), potato dextrose broth (PDB) and malt extract broth (MEB) in order to determine the microorganism with good culture characteristics as well as with amylolytic activity. The strain cultured in PDB exhibited mycelia production from 12.6 g/L (Rhizopus oryzae KACC 45714) to a maximum of 48.0 g/L (Aspergillus oryzae KACC 46959), which was 2.3~9.2 times lower than that of the strain cultured in WEB and 1.7~14.6 times lower than that of the strain cultured in MEB. Accorfing the results, We found that the commercial strains of A. oryzae Suwon, CF1001 and CF1003 had a higher dry cell mass than the wild-type strains KACC 46421, 46423, 46424 and 46959. For Rhizopus sp., the acidity levels in WEB, PDB and MEB were 0.12~0.47%, 0.22~1.0% and 0.16~0.68% (equivalent lactic acid concentration) respectively. For A. oryzae, the acidity levels were 0.06~0.11%, 0.03~0.04% and 0.06~0.08% (equivalent lactic acid concentration), respectively. Amylase enzyme from Rhizopus delemar KACC 46419 exhibited an enzyme activity of 0.013 U and 0.019 U in WEB and MEB cultures, respectively. The enzyme activity of the amylase enzyme from A. oryzae was 0.019~0.037, 0.017~0.033 and 0.028~0.046 U in WEB, PDB and MEB cultures, respectively.

Fabrication of Reverse Osmosis Membrane with Enhanced Boron Rejection Using Surface Modification (표면개질을 이용하여 붕소 제거율이 향상된 역삼투막의 제조)

  • Lee, Deok-Ro;Kim, Jong Hak;Kwon, Sei;Lee, Hye-Jin;Kim, In-Chul
    • Membrane Journal
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    • v.28 no.2
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    • pp.96-104
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    • 2018
  • With the rapid increase in seawater desalination, the importance of boron rejection is rising. This study was conducted to investigate the effect of hydrophilic compounds on surface modification to maximize water flux and increase boron rejection. First, polyamide active layer was fabricated by interfacial polymerization of polysulfone ultrafiltration membrane with M-phenylenediamine (MPD) and trimesoyl chloride (TMC) to obtain Control polyamide membrane. Next, D-gluconic acid (DGCA) and D-gluconic acid sodium salt (DGCA-Na) were synthesized with glutaraldehyde (GA) and hydrochloric acid (HCl) by modifying the surface of Control polyamide membrane. XPS analysis was carried out for the surface analysis of the synthesized membrane, and it was confirmed that the reaction of surface with DGCA and DGCA-Na compounds was performed. Also, FE-SEM and AFM analysis were performed for morphology measurement, and polyamide active layer formation and surface roughness were confirmed. In the case of water flux, the membrane fabricated by the surface modification had a value of 10 GFD or less. However, the boron rejection of the membranes synthesized with DGCA and DGCA-Na compounds were 94.38% and 94.64%, respectively, which were 12.03 %p and 12.29 %p larger than the Control polyamide membrane, respectively.

Nonlinear Finite Element Analysis of the Reinforced Concrete Panel using High-Strength Reinforcing Bar (고강도 철근을 사용한 철근콘크리트 패널의 비선형 유한요소해석)

  • Cheon, Ju-Hyun;Seong, Dae-Jung;Cho, Hong-Jae;Cho, Jae-Yeol;Shin, Hyun-Mock
    • Journal of the Korea Concrete Institute
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    • v.27 no.5
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    • pp.481-488
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    • 2015
  • The purpose of this study is to provide analytical method to reasonably predict the overall behavior up to destruction of reinforced concrete panel specimens using high-strength reinforcing bar. A total of 12 specimens of reinforced concrete panels with a wall thickness one-third the size of the actual nuclear containment structures under various loading conditions and design parameters were selected and the analysis was performed using a non-linear finite element analysis program (RCAHEST) was developed by the authors. The mean and coefficient of variation for shear strength at cracking point and maximum shear strength from the experiment and analysis results was predicted 1.03 and 12%, 0.97 and 9%, respectively. For the shear strain at the maximum shear strength from the experiment and analysis results was predicted 0.96 and 30%, respectively. Based on the results, the analysis program that was applied newly modified constitutive equation in this study is judged as having a relatively high reliability for the analysis results.

A study of beam hardening effect reduction occur in brain CT (Brain CT에서 발생하는 선속경화현상 감소방안에 관한 연구)

  • Kim, Hyeon-ju
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.12
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    • pp.8479-8486
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    • 2015
  • This study aim is occur in brain CT cause of beam hardening effect and reducing method, We will scan Bone opaque bead phantom on variation of image on the influence factor with equipment called 'Samatom Senation 16' with following listed herein : tube voltage, tube current, slice thickness, gantry angle, base line which affect beam-hardening effect. After that we are going to start Quantitative Analysis resulted in previous scanning and Qualitative Assessment with CT image sheet evaluation. result of quantitative analysis 140kVp $31.56{\pm}2.89HU$ on tube voltage, 150mA $-3.87{\pm}0.12HU$ on tube current, 3mm on slice thickness, and $13.31{\pm}1.03HU$ IOML on gantry angle which was the least beam-hardening effect. Like Qualitative Analysis, we went through Qualitative Assessment and most of valuers got a result of 140kVp on tube voltage, 150mA on tube current, 3mm on slice thickness. As before valuers evaluated gantry angle that scanned image from IOML or OML was the least beam-hardening effect occured. There are meaningful differences when we compare all theses factors statistically(P<0.05). therefore We consider that Minimizing artifact that caused by beam-hardening effect can provide better quality of image to deciphers and patients. if we rise tube voltage in permissible dose limit, set tube current in a limit that does not effect to image quality, use slice thickness too thin enough to harm resolution, use IOML or OML on gantry angle.

Synthesis and Photoluminescence Properties of Y1-x(P1-y-zNbyVz)O4:Eux Phosphors by Modified Combinatorial Chemistry Method (조합화학 기법을 이용한 Y1-x(P1-y-zNbyVz)O4:Eux 형광체의 합성 및 빛 발광 특성)

  • Zeon, Il-Woon;Sohn, Kee-Sun;Park, Hee-Dong
    • Journal of the Korean Chemical Society
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    • v.46 no.1
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    • pp.69-75
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    • 2002
  • The $Y_{1-x}(P_{1-y-z}Nb_yV_z)O_4:Eu_x$ blue and red emitting phosphors were prepared by the combinatorial chemistry method. The combinatorial library was designed to investigate the luminescence of the $Y_{1-x}(P_{1-y-z}Nb_yV_z)O_4:Eu_x$ phosphors under 254 nm and 147 nm excitations. In addition, the crystallinity and morphology of phosphors were checked by XRD and SEM. Based on the results from the combinatorial screenings, luminescent properties of phosphors are strongly dependent on the concentration of doping metal ions. It was found that a new phosphor $Y_{0.88}(P_{0.92}Nb_{0.05}V_{0.03})O_4:Eu_{0.12}$ shows excellent luminescent efficiency comparing to the $Y_{0.88}PO_4:Eu_{0.12}$ red phosphor.

Technical Development of Hot Sauce with Red Pepper (생홍고추를 이용한 핫소스 제조 기술)

  • Kwon, Dong-Jin;Kim, Yoo-Jin;Lee, Sung;Yoo, Jin-Yong
    • Korean Journal of Food Science and Technology
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    • v.30 no.2
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    • pp.391-396
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    • 1998
  • To inhibit the import of hot sauce, the manufacturing process of the Korean type hot sauce was developed. Chemical analysis of the imported hot sauce showed as follows; moisture 95.66% (w/w), salt 2.79% (w/w), titratable acidity 3.62% (w/w), crude fiber 0.42% (w/w), reducing sugar 0.03% (w/w), capsanthin 0.89% (w/w), capsaicin 4.28 mg% and pH 3.26. Korean type hot sauce prepared with red pepper, salt and vinegar was aged in oak barrel for 12 months at $10^{\circ}C$. Chemical analysis of the Korean type hot sauce showed as follows; moisture 92.05% (w/w), salt 8.82% (w/w), titratable acidity 3.88% (w/w), crude fiber 3.14% (w/w), reducing sugar 1.78% (w/w), capsanthin 0.13% (w/w), capsaicin 2.32 mg% and pH 3.29. Among chemical components, the contents of reducing sugar, salt and crude fiber except capsaicin and capsanthin were more than those of imported one. Chemical components of the Korean type hot sauce were changed slightly during aging for 12 months. The cell counts of yeast were increased from $3.2{\times}10^2\;to\;5.2{\times}10^6$ till the second month and those of lactic acid bacteria were increased from $3.{\times}10^1\;to\;1.5{\times}10^7$ till the third month, those of yeast and lactic acid bacteria decreased from the eighth month showed $6.6{\times}10^4$ and not detectable on the twelfth month, respectively. As the results of sensory evaluation on the Korean type hot sauce were evaluated to be superior to the imported to be superior to the imported one.

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A Effect on Physiological Metabolism of Microorganism Which Irradiated by Non-ionization Radiation (비 전리 방사선을 조사한 미생물에서 생리적 대사에 미치는 영향)

  • Ko, In-Ho
    • Journal of the Korean Society of Radiology
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    • v.12 no.3
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    • pp.381-387
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    • 2018
  • A Effect on physiological metabolism of microorganism which irradiated by visible light of non-ionization radiation(12,000 Lux) was investigated. The microorganism used in this experiment was a Rhodospirillum rubrum KS-301 of chemosynthetic microorganism. Batch fermentation of glucose were implement, and based on the data resulted from the fermentation. First, physiological characteristic of microorganism which was not irradiated was investigated. As a result, the decrease of the residual quantity of the substance(5.03 g/L - 2.17 g/L) was increased with the quantity of the bacteria(1.08 g/L - 3.14 g/L)and the quantity of the hydrogenous production(0.186 g - 0.3 g) respectively. Second, physiological characteristic of microorganism which was irradiated was investigated. As a result, the decrease of the residual quantity of the substance(13.17 g/L - 5.2 g/L) was increased with the quantity of the bacteria(4.7 g/L - 10.57 g/L)and the quantity of the hydrogenous production(0.186 g - 0.3 g) respectively. As the physiological characteristic of microorganism which was irradiated by visible light of non-ionization radiation(12,000 Lux) was active with its life, but the cell damages irradiated by with gamma-ray, X-ray, electron-ray in ionization radiation were appeared at cell.

Hemoglobin Concentration in Female Workers of Occupational Exposure to Lead (연 취급 근로자들의 혈색소치의 변동)

  • Park, Nan-Kyu;Kang, Pock-Soo;Chung, Jong-Hak
    • Journal of Yeungnam Medical Science
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    • v.3 no.1
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    • pp.73-79
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    • 1986
  • This study was conducted to evaluate the effects of lead on the incidence of anemia and to find cases with lead poisoning early among female workers who have been exposed to lead occupationally, for one year from August, 1985. The level of blood lead and hemoglobin concentration were observed for 284 female lead workers and 123 female non-lead workers of industries in the Gumi industrial complex in Kyungpook Province. The average age was $20.3{\pm}2.9$years and $21.1{\pm}3.5$ years in lead workers and non-lead workers, respectively. The average working duration was $26.46{\pm}19.26$ months in lead workers. The mean value of blood lead was $30.11{\pm}6.61{\mu}g/100ml$ and $21.86{\pm}3.75{\mu}g/100ml$ in lead workers and non-lead workers, respectively. There were significant differences between two groups. The mean value of hemoglobin concentration was $14.00{\pm}0.57g/100ml$ and $14.03{\pm}0.64g/100ml$ in lead workers and non-lead workers, respectively. Anemia cases were not observed at Hb concentration below 12g/100ml. The percentages of lead workers at Hb concentration ranged 12.0~13.0g/100ml were 4.5%. There was no dose-response relationship between blood lead level and the incidence of anemia. There were no remarkable differences between age and blood lead level as well as Hb concentration, and between working duration and the level of blood lead and hemoglobin.

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Correlation of Refractive Error, Axial Length, Chamber Depth, Lens Thickness and Corneal Thickness of Normal University Students (정상 대학생의 눈 굴절이상, 안축장, 전방깊이, 수정체두께 그리고 각막두께의 연관성)

  • Kim, Chang-Sik;Lee, Hak-Jun
    • Journal of Korean Ophthalmic Optics Society
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    • v.13 no.1
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    • pp.89-94
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    • 2008
  • Purpose: To make a comparative study of correlation between biometry data of size in eyeball and refractive error. Methods: The subjects were 68 normal university students (male 36, female 32) and the average age was 22.85${\pm}$3.12. We measured the students' eyesight by A-scan ultrasound and refractor. The results were examined it's statistical significance by SPSS 12.0 version. Results: The mean of axial length was 24.31${\pm}$1.24 mm, chamber depth was 3.48${\pm}$0.28 mm, lens thickness was 3.56${\pm}$0.26 mm and corneal thickness was 0.55${\pm}$0.03 mm. Male's Axial length and chamber depth were larger than female's. As reflective error decreases the thickness of lens become thicker. The measurement data between right eye and left eye didn't had difference and there was no correlation with result of T-test. There were statistically significant correlation with length and chamber depth, axial length and corneal thickness, chamber depth and corneal thickness, and refractive error and lens thickness (p<0.01). Refractive error and axial length were minus linear regression (r=-0.56). Conclusions: Eye's refractive error was changed by axial length, chamber depth and lens thickness but it wasn't related with sex and whether it is a right eye or a left eye.

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Effects of Suspended Solid and Cadmium on the Shallow-sea Foodweb Ecosystem -1. Reduction of Growth Rate and Biomass Yield of Coastal Diatom Clones by Cadmium- (천해역 먹이망 생태계에 대한 무기부유입자와 카드뮴의 영향 -I. 연안역 규조류 단종배양체의 성장률과 생체량증가에 대한 카드뮴의 저해효과-)

  • YIH Wonho;YANG Jae Sam;JO Soo-Gun;CHUNG Ee-Yung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.4
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    • pp.373-379
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    • 1994
  • Final biomass yields(cells/ml) and growth rates(divisions/day) of 4 clones of marine diatoms isolated from the Korean coastal waters were measured in media with 6 different levels of added cadmium concentrations. A neritic diatom, GS-12(Chaetoceros sp.), showed no growth at 0.1ppm cadmium, and its $IC_{50}$ for final biomass yield and growth rate was 0.03 and 0.02ppm, respectively. Two clones isolated from tidal pool, NC-37 and NC-29, showed enhanced tolerance to cadmium toxicity. Extremely high tolerance to cadmium addition was found in J-21 from a eutrophicated bay, with its high $IC_{50}$ for biomass yield(1.07ppm) and growth rate(1.92ppm). Present results implied a habitat related pattern of coastal diatom clones in the cadmium tolerances. Except GS-12, the other three diatom clones are considered to be highly tolerant to cadmium stresses.

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