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The effect of chemicals and chilling treatments on the frequency of metaphase in root tips of some economic crops (화학약품과 저온처리가 작물근단세포의 Metaphase 출현빈도에 미치는 영향)

  • Mun-Hue Heu;Hye-Yong Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.2
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    • pp.3-9
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    • 1964
  • Root tips of wheat, soybean, cotton and barley were treated with cold temperature (12 or 24 hrs in $0^{\circ}C$ or 2$^{\circ}C$), 8-Hyd.exyquinolin (2 or 10 hrs in 0.03 or 0.1%) and colcllicine (2 or 10 hrs in 0.2 or 1, 0%), and the frequency of metaphase were observed. The results were summarized as follow; 1. Chilling the seminal roots before or after sooting from the seed significantly increased tile number of mitotic cells and the rate of metaphase cells to mitotic cells. The optimal duration of chilling seemed to be differ depending on the kinds of plant and 24 hours to be too long except wheat so far examined here. 9. 8-Hydroxyquinolin treatment, about 2 hours in 0.03%, increased the rate of metaphase cells. The higher concentration and the longer treatment of this chemical caused the lower frequency of mitotic cells generally. 3. Colchicine treatment, 2 to 10 hrs in 0.2 to 1.0%, increased the frequency of mitotic cells and the rate of metaphase cells. Colchicine treatment was same or superior than any other treatments on the increase of metaphase cells. 4. All the treatments examined here caused chromosome contraction with most serious in colchicine following by 8-Hydroxyquinolin and chilling. 5. The feasibility of general application of chilling under the check of proper temperature and proper duration depending on the kinds of crops were discussed.

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Quantification of Thermal Insulation by Clothing Items and Analysis of Influencing Factors (단일의복의 보온력 정량화와 영향 요인)

  • Baek, Yoon Jeong;Hwang, Soo Kyung;Lee, Hyo Hyun;Park, Joonhee;Kim, Do-Hee;Lee, Joo-Young
    • Journal of the Korean Society of Clothing and Textiles
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    • v.42 no.1
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    • pp.172-182
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    • 2018
  • The purpose of the present study was to quantify the thermal insulation of garments by item and examine factors influencing clothing insulation. A total of 769 garments in clo unit were collected and classified into 12 categories: blouses/shirts (95 items, BS), T-shirts/sweaters (62 items, TS), vest (23 items, VT), cardigans (23 items, CD), jackets/coats (75 items, JC), sport outerwear (including padding jackets)(48 items, SO), trousers (23 items, TR), skirts (56 items, SK), dresses (28 items, DS), underwear (150 items, UW), sleepwear (50 items, SW), and personal protective clothing (59 items, PPC). The results showed that clothing insulation was $0.21{\pm}0.01clo$ for the BS, $0.22{\pm}0.01clo$ for TS, $0.12{\pm}0.00clo$ for VT, $0.23{\pm}0.02clo$ for CD, $0.40{\pm}0.02clo$ for JC, $0.49{\pm}0.03clo$ for SO, $0.21{\pm}0.01clo$ for TR, $0.18{\pm}0.01clo$ for SK, $0.34{\pm}0.03clo$ for DS, $0.09{\pm}0.01clo$ for UW, $0.42{\pm}0.03clo$ for SW, and $0.56{\pm}0.03clo$ for PPC (p<.001). The most influential factors among the seven factors for thermal insulation of garments were clothing weight and covering area; however, the explanatory powers of two factors differed according to clothing categories. The covering area had more significant impact on clothing insulation in cardigans, jackets/coats, trousers, and dresses than clothing weight. Covering areas and clothing weight were the most influential factors in the following categories: blouses/shirt, T-shirts/sweaters, skirts, sleepwear and personal protective clothing. The garment weight was the most important factor for thermal insulation for the sport outerwear.

The Effect of Chordae Preservation in Mitral Valve Replacement (승모판막 치환술에 있어 건삭 보존의 효과)

  • 김공수;조중구;구자홍;김태호
    • Journal of Chest Surgery
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    • v.32 no.4
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    • pp.353-357
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    • 1999
  • Background: Mitral valve replacement(MVR) with chordal preservation in patients with mitral vlavular disease has been proven to be beneficial for left ventricular function and for reduction of postoperative complication. Material and Method: From January 1995 to July 1996, the early postoperative results of mitral valve replacement were compared between 20 patients who underwent chordae resection(classic MVR group) and 10 patients who underwent chordae preservation(preservation MVR group) in the Department of Thoracic and Cardiovascular Surgery, Chunbuk National University Hospital. Result: There was no significant difference between the two groups in age, sex, NYHA functional class, cardiothoracic ratio, echocardiographic finding, cardiopulmonary bypass time and aortic cross clamping time. The difference between preoperative and postoperative cardiothoracic ratio after 3 months was not statistically significant. At echocardiographic left ventricular evaluation, ejection fraction and fractional shortening decreased slightly in the preservation group then preoperative value (p=0.47, p=0.12), however, decreased significantly in the classic MVR group(p=0.03, p=0.04), and were statistically significant between the two groups(p=0.03, p=0.02). Conclusion: We conclude that MVR with chorda preservation seems to have a beneficial effect on postoperative left ventricular performance in mitral valve disease than the classic MVR.

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Seasonal variation in the body composition, amino acid, fatty acid and glycogen contents of triploid Pacific oyster, Crassostrea gigas in western coastal waters of Korea (서해안 3배체 굴 (Crassostrea gigas) 의 계절별 체성분, 아미노산, 지방산 및 글리코겐 함량 변화)

  • Hwang, In Joon;Han, Jong Chul;Hur, Young Baek;Lim, Hyun Jeong
    • The Korean Journal of Malacology
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    • v.32 no.4
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    • pp.269-277
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    • 2016
  • We examined the seasonal variation of the proximate, amino acid, fatty acid composition and glycogen contents of triploid Pacific oyster cultured in western coastal waters of Korea in 2015. The specimens were collected in February, May, August and November from off-bottom farming site. The lipid content was the highest in winter season (February, $2.10{\pm}0.06%$), the protein content was the highest in autumn season (November, $9.09{\pm}0.04%$) while the moisture and ash content was the highest in summer season (August, $86.00{\pm}0.12%$ and $2.67{\pm}0.03%$, respectively). The total amino acid contents was the highest in winter season (1,011.5 mg / 100 g), decreased gradually and was the lowest in summer season (486.7 mg / 100 g). The total amino acid composition was dominated by taurine, aspartic acid, glutamic acid, glycine and alanine. In the fatty acid composition, the two fatty acids, eicosapentaenoic acid and docosahexaenoic acid, which comprised that as much as 50% of the total fatty acid contents, were dominated during all season. The glycogen content was the highest in summer season ($1.26{\pm}0.03g$ / 100 g) although it was stable during the other season with the range of $0.69{\pm}0.04-0.86{\pm}0.01g$ / 100 g. Taken together, these results suggest that triploid pacific oysters have high commercial value not only in winter season but in summer season because they do not spawn and have high contents of glycogen comparing with those of diploid oyster in this period.

Relationship between Hypertension and the Declining Renal Function in Korean Adults (한국 성인에서 고혈압과 신기능 저하와의 관련성)

  • Lee, Jun Ho
    • Korean Journal of Clinical Laboratory Science
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    • v.53 no.1
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    • pp.32-40
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    • 2021
  • This study examined the association between hypertension (HTN), estimated glomerular filtration rate (eGFR), and urine microalbumin/creatinine ratio (ACR) in Korean adults. Data for 8,922 adults (3,941 men and 4,981 women) aged ≥20 years from the Sixth Korean National Health and Nutrition Examination Survey VI (2013~2014) were analyzed. In men, after adjusting for the related variables, the odds ratios (ORs) of HTN [systolic blood pressure (SBP) ≥140 mmHg, diastolic blood pressure (DBP) ≥90 mmHg, or use of HTN medications] were significantly higher in the decreased eGFR group [eGFR <60 mL/min/1.73 ㎡, 1.98 (95% CI, 1.21~3.24)], elevated ACR group [ACR ≥30 mg/g, 2.03 (95% CI, 1.54~2.69)], and decreased eGFR plus elevated ACR group [eGFR <60 mL/min/1.73 ㎡ and ACR ≥30 mg/g, 6.03 (95% CI, 2.82~12.92)] than in the normal group (eGFR ≥60 mL/min/1.73 ㎡ and ACR <30 mg/g). In women, after adjusting for the related variables, the ORs of HTN were significantly higher in the decreased eGFR group (2.29, 95% CI, 1.27~4.13), elevated ACR group (2.22, 95% CI, 1.68~2.94), and decreased eGFR plus elevated ACR group (10.77, 95% CI, 3.89~29.82) than the normal group. In conclusion, HTN was associated with a decreased eGFR and elevated ACR in Korean men and women. In addition, the prevalence of HTN increased greatly when a decreased eGFR and elevated ACR occurred simultaneously.

Changes of Phytochemicals and Antioxidant Activity during Fermentation of Brown Soymilk (갈색콩 두유의 젖산 발효 중 phytochemicals 및 항산화 활성 변화)

  • Hwang, Chung Eun;Lee, Byong Won;An, Min Ju;Lee, Hee Yul;Kim, Hyun Tae;Ko, Jong Min;Baek, In Youl;Cho, Kye Man
    • Journal of agriculture & life science
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    • v.50 no.4
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    • pp.157-167
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    • 2016
  • The changes of total phenolic and isoflavone contents and antioxidant activities in of brown soymilk fermented by Lactobacillus plantarum P1201 were investigated. The brown soymilk proliferated the cell growth and reached about 11.55log cfu/g after fermentation for 60hr, while pH and titratable acidity ranged from 6.25 to 4.03 and 0.18% to 1.03%, respectively. The total phenolic contents of brown soymilk slightly increased from 2.87mg/g to 2.98mg/g after fermentation for 60hr. The levels of isoflavone-glycosides and -malonylglycosides decreased, while the isoflavone-aglycone contents increased during fermentation of brown soymilk. In particular, the isoflavone contents was 38.30㎍/g, but increased the highest value of 84.31㎍/g after fermentation for 60hr. After then, it was slightly decreased after 60hr of fermentation. In addition, the levels of daidzein, glycitein and genistein among isoflavone aglycones were 24.12㎍/g, 25.25㎍/g and 24.71 ㎍/g, after fermentation for 36hr. The DPPH and ABTS radical scavenging activities and FRAP assay showed to be slightly increased during lactic acid fermentation of brown soymilk.

The Mediating Effect of Affect in the Relationship between Emotional Intelligence and Organizational Commitment among General Hospital Nurses (종합병원 간호사의 감성지능과 조직몰입의 관계에서 정서의 매개효과)

  • Yoonjeong Lee;Moonkyoung Park
    • Journal of Industrial Convergence
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    • v.22 no.4
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    • pp.57-64
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    • 2024
  • This study was to confirm the effect of emotional intelligence on organizational commitment of general hospital nurses and the mediating effect of affect. Data was collected using a structured online self-report questionnaire on emotional intelligence, organizational commitment, and both negative and positive affect from 236 nurses working in general hospitals. The data collected were analyzed using descriptive statistics, Pearson's correlation, and a parallel multiple mediation model. This analysis was conducted with IBM SPSS Statistics (Version 27.0) and the PROCESS macro (Model 4). This study's findings revealed that emotional intelligence was significant correlated with positive affect, negative affect, and organizational commitment. Emotional intelligence had a significant direct effect on positive affect (β=.16, p=.015), negative affect (β=-.28, p<.001), and organizational commitment (β=.33, p<.001). Positive affect (β=.20, p=.001) and negative affect (β=-.25, p<.001), had a significant direct effect on organizational commitment. And the mediating effect of positive affect (β=.03, 95% bootstrap CI=0.01~0.07) and negative affect (β=.07, 95% bootstrap CI=0.03~0.12) was also significant. Based on these research results, it will be necessary to research various training programs that can manage emotional intelligence and affect together in developing programs to improve nurses' organizational commitment.

Photosynthetic Characteristics of Benthic Microalgae Measured by HPLC and Diving Pulse Amplitude Modulated (PAM) Fluorometry on the Nakdong River Estuary of the Korean Peninsula (HPLC 및 Diving-PAM을 이용한 낙동강 하구 저서미세조류의 광합성 특성)

  • Jeong Bae Kim;Mi Hee Chung;Jung-Im Park
    • Korean Journal of Ecology and Environment
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    • v.57 no.2
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    • pp.61-74
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    • 2024
  • Daemadeung, located in the estuary of the Nakdong River, is formed by sand dunes and possesses well-developed intertidal flats. This study aimed to investigate the habitat of benthic microalgae, photosynthetic pigments, and photosynthetic efficiency in the intertidal flats of Daemadeung from January to December 2011. The inorganic nitrogen content in the sediment pore water was primarily composed of ammonium, while nitrate + nitrite was dominant in the upper layer water. The concentration of chlorophyll a and fucoxanthin in the sediment surface was significantly higher than the mean of all the sediment layer. The average Fv/Fm of benthic microalgae during the entire survey period was 0.52±0.03, with the highest value (0.61±0.08) observed in February. The rETRmax showed a seasonal trend, being high from spring to early autumn (April to October) and low from winter to early spring (January to March, November, December), with the highest value (153.05±2.30 µmol electrons m-2 s-1) in July and the lowest (38.49±5.17 µmol electrons m-2 s-1) in January. The average Fv/Fm of diurnal microalgae was 0.48±0.03, with the highest value (0.61±0.08) observed at noon. The rETRmax showed a highest peak at noon (54.24±11.35 µmol electrons m-2 s-1) and reached its lowest point at 16:00 (26.17±4.75 µmol electrons m-2 s-1). These findings suggest that the productivity of benthic microalgae varies significantly depending on the survey time and sediment depth. Therefore, to quantify the productivity of benthic microalgae using Diving-PAM, surveys should be conducted based on tidal conditions, and simultaneous pigment analysis of sediment layers should also be performed.

Studies on the production of lysine by fermentation process (1) -Isolation of lysine producing microorganisms and cultural conditions of lysine accumulation- (발효에 의한 라이신(L-lysine) 생산에 관한 연구(1) -라이신 생산균주의 분리 및 라이신 생산조건의 검토-)

  • Mheen, Tae-Ick;Kwon, Tai-Wan
    • Korean Journal of Food Science and Technology
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    • v.3 no.1
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    • pp.68-77
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    • 1971
  • Ninty four strains of lysine producing micro-organisms in culture broth during fermentation have been isolated from soil and other sources. From the comparison of the amounts of lysine produced, 6 strains have been selected as the potentially useful strains, and identified tentatively as Micrococcus sp. (S-16-4), Corynebactcrium sp. (S-27-12, S-281-3, CBY-4) and Brevibacterium sp. (M-6-71, F-629-2), respectively. From the further studies with Corynebacterium sp., S-27-12, its maximum yield was found to be 4mg lysine/ml of synthetic medium, consist of glucose(7.5%), urea(0.6%), $KH_2PO_4(0.2%)$, $Na_2HPO_4(0.05%)$, $MgSO_4{\cdot}7H_2O(0.03%)$, $MnSO_4{\cdot}4H_2O(0.001%)$ and $FeSO_4{\cdot}7H_2O(0.0005%)$ at pH 7.2 and $30^{\circ}C$ after 4 days.

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Microstructure and Electrical Properties of Vanadium-doped ${Bi_4}{Ti_3}{O_{12}}$ Thin Films Prepared by Sol-gel Method (졸-겔법으로 성장시킨 바나듐이 도핑된 ${Bi_4}{Ti_3}{O_{12}}$ 박막의 미세구조 및 전기적 특성)

  • Kim, Jong-Guk;Kim, Sang-Su;Choe, Eun-Gyeong;Kim, Jin-Heung;Song, Tae-Gwon;Kim, In-Seong
    • Korean Journal of Materials Research
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    • v.11 no.11
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    • pp.960-964
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    • 2001
  • $Bi_{3.99}Ti_{2.97}V_{0.03}O_{12}$ (BTV) thin films with 3 mol% vanadium doping were Prepared on $Pt/Ti/SiO_2/Si$ substrate by sol-gel method. X-ray diffraction analysis indicated that single-phase layered perovskite were obtained and preferred orientation was not observed. Under the annealing temperature at $600^{\circ}C$, the surface morphology of the BTV thin films had fine-rounded particles and then changed plate-like at $650^{\circ}C$ and $700^{\circ}C$. The remanent polarization $(2P_r)$ and coercive field $(2E_c)$ of $700^{\circ}C$ annealed BTV thin film were 25 $\mu$C/cm$^2$ and 116 kV/cm, respectively. In addition, BTV thin film showed little polarization fatigue during $10_9$ switching cycles. These improved ferroelectric properties were attributed to the increased rattling space and reduced oxygen vacancies by substitution $Ti^{4+}$ ion (68 pm) with smaller $V^{5+}$ ion (59 pm). The dielectric constant and loss were measured 130 and 0.03 at 10 kHz, respectively.

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