• The Plant Pathology Journal
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• 제15권1호
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• pp.21-27
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• 1999

Nonpathogenic mutants of Xanthomonas campestris pv. glycines were generated with Omegon-Kim to isolate genes essential for pathogenicity and inducing hypersensitive response (HR). Three nonpathogenic multants and two mutants showing slow symptom development were isolated among 1,000 colonies tested. From two nonpathogenic mutants, 8-13 and 26-13, genes homologous to hrcC and hrpF of X. campestris pv. vesicatoria were identified. The nonpathogenic mutant 8-13 had a mutation in a gene homologous to hrpF of X. campestris pv. vesicatoria and failed to cause HR on pepper plants but still induced HR on tomato leaves. The nonpathogenic mutant 26-13 had an insertional mutation in a gene homologous to hrcC of X. campestris pv. vesicatoria and lost the ability to induce HR on pepper leaves but still caused HR on tomato plants. Unlike other phytopathogenic bacteria, the parent strain and these two mutants of X. campestris pv. glycines did not cause HR on tobacco plants. a cosmid clone, pBL1, that complemented the phenotypes of 8-13 was isolated. From the analysis of restriction enzyme mapping and deletion analyses of pBL1, a 9.0-kb Eco RI fragment restored the phenotypes of 8-13. pBL1 failed to complement the phenotypes of 26-13, indicating that the hrcC gene resides outside of the insert DNA of pBL1. One nonpathogenic mutant, 13-33, had a mutation in a gene homologous to a miaA gene encoding tRNA delta (2)-isopentenylpyrophosphate transferase of Escherichia coli. This indicated that tRNA modifications in X. campestris pv. glycines may be required for expression of genes necessary for pathogenicity. The mutant 13-33 multiplied as well as the parent strain did in the culture medium and in planta, indicating that loss of pathogenicity is not due to the inability of multiplication in vivo.

• 한국정보전자통신기술학회논문지
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• 제1권1호
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• pp.47-52
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• 2008

본 논문에서는 LTCC를 이용한 이중대역을 갖는 소형화된 칩 PIFA를 설계하였다. 제안된 PIFA 는 K-PCS 대역과 WiBro 대역에서 동작시키기 위하여 이중 공진 특성을 갖도록 설계 제작 하였다. 본 안테나는 두개의 층으로 이루어져 있으며 아래 패치는 1.727 ~ 1.847GHz 의 약 120 MHz 대역폭을 갖으며 K-PCS 대역을 만족한다. 위 패치로의 급전을 위해 적층 급전을 이용하였으며 2.302 ~ 2.412 GHz 의 약 110 MHz 대역폭으로써 WiBro 대역을 만족한다. K-PCS 와 WiBro 대역에서 최대 방사 이득은 각각 2.11, 3.71 dBi로 나타났다. 안테나의 소형화를 위해 유전율 8을 갖는 유전체 LTCC를 사용하여 칩 형태로 제작하였다. 제안된 PIFA는 SAR 저감효과를 나타내었다.

• Molecules and Cells
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• 제42권10호
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• pp.711-720
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• 2019

Sink strength optimizes sucrose import, which is fundamental to support developing seed grains and increase crop yields, including those of rice (Oryza sativa). In this regard, little is known about the function of vacuolar invertase (VIN) in controlling sink strength and thereby seed size. Here, in rice we analyzed mutants of two VINs, OsVIN1 and OsVIN2, to examine their role during seed development. In a phenotypic analysis of the T-DNA insertion mutants, only the OsVIN2 mutant osvin2-1 exhibited reduced seed size and grain weight. Scanning electron microscopy analysis revealed that the small seed grains of osvin2-1 can be attributed to a reduction in spikelet size. A significant decrease in VIN activity and hexose level in the osvin2-1 spikelets interfered with spikelet growth. In addition, significant reduction in starch and increase in sucrose, which are characteristic features of reduced turnover and flux of sucrose due to impaired sink strength, were evident in the pre-storage stage of osvin2-1 developing grains. In situ hybridization analysis found that expression of OsVIN2 was predominant in the endocarp of developing grains. A genetically complemented line with a native genomic clone of OsVIN2 rescued reduced VIN activity and seed size. Two additional mutants, osvin2-2 and osvin2-3 generated by the CRISPR/Cas9 method, exhibited phenotypes similar to those of osvin2-1 in spikelet and seed size, VIN activity, and sugar metabolites. These results clearly demonstrate an important role of OsVIN2 as sink strength modulator that is critical for the maintenance of sucrose flux into developing seed grains.

• 한국전자통신학회논문지
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• 제14권2호
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• pp.439-446
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• 2019

본 논문에서는 효율적으로 의료 영상을 보호하기 위하여 C-MLCA와 1차원 CAT를 이용한 암호화 방법을 제안한다. 먼저, Wolfram 규칙으로 상태 전이 행렬을 생성한 후 최대 길이의 수열을 만든다. 다음으로 여원 벡터를 곱하여 복잡한 수열로 변환한다. 그리고 두 수열을 행과 열로 곱하여 원 의료 영상 크기의 기저 영상을 생성한 후 XOR 연산을 한다. 마지막으로 게이트 웨이값을 설정하여 만들어진 1차원 CAT 기저함수와 CAT 변형 계수가 적용된 영상을 연산하면, 최종적으로 암호화된 영상을 얻을 수 있다. 암호화된 영상은 원 의료 영상과 비교하기 위해 히스토그램과 PSNR을 사용하여 평가한다. 또한 NPCR과 키 공간 분석을 통하여 제안한 방법의 안정성을 검증한다.

• 대한조선학회논문집
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• 제37권1호
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• pp.82-90
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• 2000

수면하에서 정속으로 움직이는 원형실린더에 의하여 생성되는 정상파 및 쇄파에 대한 실험적 연구를 회류수조에서 수행하였다. 실린더의 몰수깊이에 따른 자유표면 형상, 물체표면 압력분포를 계측하였고 물체후류에서의 속도, 전수두손실 분포와 난류강도를 계측하였다. 총3부로 나누어 발표할 본 연구의 제1부는 실험장치와 몰수깊이에 따른 파형과 압력분포 변화를 소개하고 있다. 이 내용은 전통적인 선박유체역학 분야 종사자들에게 유익한 실험자료를 제공하는데 목적이 있다. 뒤이어 발표될 제2부는 쇄파주의 순간 유동장의 정량적 가시화를 통한 실린더와 쇄파간의 상호작용을 해석하고자 한다.

• Journal of Microbiology and Biotechnology
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• 제3권4호
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• pp.232-237
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• 1993

A strain of yeast which can convert starch directly to ethanol was developed by the intergeneric protoplast fusion between Schwanniomyces alluvius possessing $\alpha$ amylase as well as glucoamylase with debranching activity and FSC-14-75 which previously had been formed from a heterologous transformation and subsequent intergeneric protoplast fusion. Fusants were selected on minimal medium after protoplasts of auxotrophic mutant of S. alluvius fused with heat-treated protoplasts of FSC-14-75 in the presence of 30%(w/v) PEG and 20 mM $CaCl_2$. The fusion frequency was in the range of $10^{-6}$ order. All fusants tested were intermediate types of parental strains for carbon compound assimilation, and their cell volumes were approximately 1.1 times larger than FSC-14-75 and 1.8 times larger than S. alluvius. The fusants were unable to sporulate like FSC-14-75, while S. alluvius could sporulate. In flask scale the most promising fusant, FSCSa-R10-6, produced 7.83%(v/v) and 10.17%(v/v) ethanol from 15% and 20% of liquefied potato starch, respectively, indicating that the fermetation efficiency of each case increased 1.2 times and 1.6 times than that of FSC-14-75. The elution pattern on DEAE-cellulose chromatography showed that FSCSa-R10-6 has four distinct amylase peaks of which two peaks originated from S. alluvius and the other two from FSC-14-75. These results suggest that the enhanced fermentation efficiency of the fusant might be due to almost-complemented parental amylases.

• 한국환경복원기술학회지
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• 제19권1호
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• pp.135-153
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• 2016

This study is aimed at grasping improvement factors of Cheonho Park, Gandaemae Park and Hunlyunwon Park as barrier free friendly parks. For this study, current status of parks, users' state and satisfaction were analyzed in October, 2014 and September, 2015. To detect current status, period of designing, whole size, facility pavement green space area, and main spaces were examined. As for BF status, mediation facilities, leading and guidance facilities, convenient facilities and BF pedestrial passage were analyzed. Also, It examined user behavior and satisfaction by accessibility, convenience and safety items. As a result of status analysis, three parks were designed in 1997~1998. The size of parks were $15,180m^2{\sim}26,697m^2$. As for mediation facilities, the slopes of walking-passage in Cheonho Park and Gandaemae Park were flat. The rate of leading-guidance facilities was 72.7%~93.4%. In the three parks, the effective width of safe pedestrian space within BF pedestrial passages was more than 1.8m. In Cheonho Park and Gandaemae Park, more than 50% of soil and urethane were used as paving materials. Users were the most satisfied with accessibilities in Cheonho Park due to flat-typed walking passages. In convenience, when it comes to using benches and pergolas, it showed significantly different satisfaction in every park. In safety, there were also considerable difference in every park. Overall, it was analysed that the satisfaction with information for safety and facilities was significantly low. Against this backdrop, this study suggests three considerations to recreate parks as barrier free friendly parks. 1) Flat-typed walking passages and enough parking areas near parks should be constructed. 2) Additional rest furniture for convenience should be installed and complemented. 3) Walking oriented-spatial safe facilities for safety should be differently supplemented.

• 복식문화연구
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• 제12권3호
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• pp.392-406
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• 2004

The purpose of this study is to recognize of the size conditions of ready to wear skirts and analyze the patterns of them so that some educational patterns can be complemented. For this purpose, the investigation of the size conditions was focused on the body sizes and products sizes of the care labels. The patterns of them produced through CAD have been classified into 24 items for the effective and practical analysis. Thus, 9 brands were selected and the basic skirts marketed in 2002 spring were analyzed. The results were as follows. 1. Designs of the backs of the basic skirts produced by 9 brands have the similar designs with two darts. Regarding the designs of the front, 6 brands of them have the designs with no darts, 3 of them have the one with two darts. Designs of waists are composed of low waists and round belts. 2. Regarding the waist circumference as the basic composing elements of skirts, 55.6% of the patterns have the composition of the styles with larger front than the back but 33.3% of them have the same front as back. As to the hips, 77.8% of them have the composition of the patterns with larger back than the front. As for the comparison of waist lines, 55.6% of them have lower front lines than the back lines. 3. Regarding darts placement, it was moved to the side lines from the middle of 1/2 of waist lines, dart intake was 1.8-2.4㎝, the length of the front darts was 7.8-9.5㎝, and the length of the back darts 8.4-11.1㎝ 4. The results of wearing-tests by the sensory evaluation, it showed that almost all the items of the results were satisfactory.

• 한국수자원학회논문집
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• 제48권3호
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• pp.159-167
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• 2015

댐 운영에 있어서 저수지 초기 담수 시 또는 집중호우 등에 의한 급격한 수위 상승 방지나 운영 중에 댐체 점검 및 수리 또는 자연재해로 인한 위급한 상황 발생 등 저수지를 비워야 할 상황이 발생할 수 있으며, 이에 대한 대책으로 비상 시 방류할 계획 및 설비가 필요하다. 그러나 현재 국내에는 이러한 비상방류에 대한 기준이 미비한 실정이다. 본 연구에서는 댐 안정성 제고를 위해 유사시 저수지 저류량을 안전하게 배제시킬 수 있는 비상방류 설비를 저수지 모의 모형인 HEC-ResSim 모형을 이용하여 규모 및 배제일수에 대하여 모의하고 산정하였다. 저수지 규모에 따라 세 개의 댐에 대해 검토하였다. 대상댐은 저류용량을 기준으로 10억 $m^3$ 이상인 소양강댐과 1~10억 $m^3$ 댐으로 합천댐, 1억 $m^3$ 미만의 댐으로 대곡댐을 선정하여, 방류시설의 규모를 산정하고 배제일수를 모의하여 기준의 적정성과 적용 가능성에 대해 검토하였다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제31권3호
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• pp.266-273
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• 2005

Purpose: To assess the relationship between soft tissue reference line and hard tissue reference line using the standardized photographs and the posteroanterior cephalometric radiographs(P-A)in facial asymmetric patients and to compare the differences of angular measurement between normal group and asymmetry group. Methods: Normal group consisted of 44 persons with normal occlusion and normal facial morphology. Asymmetry group consisted of 90 patients with facial asymmetry. Standardized facial photographs and P-A were taken in all subjects. The horizontal reference lines were bipupillary line in photographs and latero-orbitale line in P-A respectively. The vertical reference line were the line from the midpoint of horizontal reference line perpendicularly. Angular measurement of otobasion canting, lip canting, nose deviation, chin deviation, and maxillary deviation were compared and analyzed in photographs. And angular measurement of mastoid canting, mandibular canting, nose deviation, chin deviation, and maxillary deviation were compared and analyzed in P-A. Results: 1. The variables of photographs and P-A were significantly related in the asymmetry group. 2. Significant differences between all variables except for PT2 and PA2 were shown in the asymmetry group and between PT1 and PA1, PT3 and PA3 in the normal group respectively. 3. Comparison measurement scores of angular difference between control group and experimental group concerning each variable showed significant difference except for PA1. Conclusions: Soft tissue components may not compensate for underlying skeletal imbalance in nose deviation and chin deviation. The horizontal reference lines in photographs were significant related with the P-A, but angular variables between the two studies show significant differences. Therefore, we do not recommend use photography in the assessment the facial asymmetry as complemented in the P-A.