• Magazine of the Korean Society of Agricultural Engineers
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• v.8 no.2
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• pp.1124-1140
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• 1966

'This hydraulic experiment have been practised Juk an Reservoir spillway and discharge 'channel which the province Kyong Buk was constructed and designed U. hook, for seizing all state of hydraulic. As result of the experimellt planning and making the model test, it has gained the necessary data at the amendment, projection of the most rational and economical result. 1. Project (1) Experiment project....1/30 of the discharge (2) project flood....0.01945 $m^3$/sec (rapidly) 2. Design Experiment It were sighted the water level for the nine point (L. & R. sides of No. O, L. & R. of No.1, L. side of NO.2, NO.3, No. 4 and NO.5), but it appeared each other that the lowest water level was 0.63 m at spillway (No.5) and the highest water level 0.735m less than planning water level O.75 m at No. 0. It was regarded as the phenomena appearing the difference from the calculation of the rational formular and coefficient of discharge. 3. Experiment examine E. ${\circled1}$ As a table (2) it had not a difference in comparision with design and was some lower value than design experiment's. E ..${\circled2}$) !twas same table (3) in a consequence of Experiment contracted Rocky cutting. E.${\circled3}$. ${\circled4}$ It was done amend.ment Experiment by elevating G.H. in only control point, but was not sure result as a table (2)(3)(4), and so it was changed largely in ${\circled5}$ Experiment. E. ${\circled5}$ Increasing water level was understanded to be proportion to $V^2$ in consideration of centrifugal force in the curve part and showed velocity contracting in curve the effect order's being regular in consequence of 1/6 sloped extending G.H. attached from 5 No. 0 to 1. 50 m, to S No. 0+5m. (S; discharge channel number).

• BMB Reports
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• v.38 no.1
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• pp.17-23
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• 2005

During the screening of xylanolytic enzymes from locally isolated fungi, one strain BCC14405, exhibited high enzyme activity with thermostability. This fugal strain was identified as Aspergillus cf. niger based on its morphological characteristics and internal transcribed spacer (ITS) sequences. An enzyme with xylanolytic activity from BCC14405 was later purified and characterized. It was found to have a molecular mass of ca. 21 kDa, an optimal pH of 5.0, and an optimal temperature of $55^{\circ}C$. When tested using xylan from birchwood, it showed $K_m$ and $V_{max}$ values of 8.9 mg/ml and 11,100 U/mg, respectively. The enzyme was inhibited by $CuSO_4$, EDTA, and by $FeSO_4$. The homology of the 20-residue N-terminal protein sequence showed that the enzyme was an endo-1,4-$\beta$-xylanase. The full-length gene encoding endo-1,4-$\beta$-xylanase from BCC14405 was obtained by PCR amplification of its cDNA. The gene contained an open reading frame of 678 bp, encoding a 225 amino acid protein, which was identical to the endo-1,4-$\^{a}$-xylanase B previously identified in A. niger.

• Journal of Microbiology and Biotechnology
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• v.26 no.9
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• pp.1650-1656
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• 2016

The SCO0284 gene of Streptomyces coelicolor A3(2) is predicted to encode an α-galactosidase (680 amino acids) belonging to glycoside hydrolase family 27. In this study, the SCO0284 coding region was cloned and overexpressed in Streptomyces lividans TK24. The mature form of SCO0284 (641 amino acids, 68 kDa) was purified from culture broth by gel filtration chromatography, with 83.3-fold purification and a yield of 11.2%. Purified SCO0284 showed strong activity against p-nitrophenyl-α-D-galactopyranoside, melibiose, raffinose, and stachyose, and no activity toward lactose, agar (galactan), and neoagarooligosaccharides, indicating that it is an α-galactosidase. Optimal enzyme activity was observed at 40℃ and pH 7.0. The addition of metal ions or EDTA did not affect the enzyme activity, indicating that no metal cofactor is required. The kinetic parameters V_max and K_m for p-nitrophenyl-α-D-galactopyranoside were 1.6 mg/ml (0.0053 M) and 71.4 U/mg, respectively. Thin-layer chromatography and mass spectrometry analysis of the hydrolyzed products of melibiose, raffinose, and stachyose showed perfect matches with the masses of the sodium adducts of the hydrolyzed products, galactose (M+Na, 203), melibiose (M+Na, 365), and raffinose (M+Na, 527), respectively, indicating that it specifically cleaves the α-1,6-glycosidic bond of the substrate, releasing the terminal D-galactose.

• Journal of Microbiology and Biotechnology
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• v.27 no.1
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• pp.9-18
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• 2017

Nine bacilli with fibrinolytic activities were isolated from doenjang, a traditional Korean fermented soy food. Among them, RSB34 showed the strongest activity and was identified as Bacillus amyloliquefaciens by 16S rRNA and recA gene sequencing. During growth on LB up to 96 h, RSB34 showed the highest fibrinolytic activity ($83.23mU/{\mu}l$) at 48 h. Three bands of 23, 27, and 42 kDa in size were observed when the culture supernatant was analyzed by SDS-PAGE and 27 and 42 kDa bands by fibrin zymography. The gene encoding the 27 kDa fibrinolytic enzyme AprE34 was cloned by PCR. BLAST analyses confirmed that the gene was a homolog to genes encoding AprE-type proteases. aprE34 was overexpressed in Escherichia coli BL21(DE3) using pET26b(+). Recombinant AprE34 was purified and examined for its properties. The $K_m$ and $V_{max}$ values of recombinant AprE34 were $0.131{\pm}0.026mM$ and $16.551{\pm}0.316{\mu}M/l/min$, respectively, when measured using an artificial substrate, N-succinyl-ala-ala-pro-phe-p-nitroanilide. aprE34 was overexpressed in B. subtilis WB600 using pHY300PLK. B. subtilis transformants harboring pHYRSB34 (pHY300PLK with aprE34) showed higher fibrinolytic activity than B. amyloliquefaciens RSB34.

• Journal of Microbiology and Biotechnology
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• v.16 no.11
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• pp.1753-1759
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• 2006

Paenibacillus sp. HY-8 isolated from the digestive tracts of the longicorn beetle, Moechotypa diphysis, produced an extracellular endoxylanase with a molecular weight of 20 kDa estimated by SDS-PAGE. The xylanase was purified to near electrophoretic homogeneity from the culture supernatant after ammonium sulfate precipitation, gel filtration, and ionexchange chromatography. The purified xylanase exhibited the highest activities at pH 6.0 and $50^{\circ}C$. The $K_m\;and\;V_{max}$ values were 7.2 mg/ml and 16.3 U/mg, respectively, for birchwood xylan as the substrate. Nucleotide sequence of the PCR-cloned gene was determined to have the open reading frame encoding a polypeptide of 212 amino acids. The N-terminal amino acid sequence and the nucleotide sequence analyses predicted that the precursor xylanase contained a signal peptide composed of 28 amino acids and a catalytically active 19.9-kDa peptide fragment. The deduced amino acid sequence shared extensive similarity with those of the glycoside hydrolase family 11 of xylanases from other bacteria. The predicted amino acid sequence contained two glutamate residues, previously identified as essential and conserved for active sites in other xylanases of the glycoside hydrolase family 11.

• Journal of Arbitration Studies
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• v.19 no.3
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• pp.161-178
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• 2009

This article focuses on the enforceability of arbitration agreements m the dispute of standby letter of credit, especially with the case analysis of the leading case from the U.S. Bankruptcy Court. In Nova Hut a.s. v. Kaiser Group International Inc. case, while the underlying contract contained an arbitration clause, a guarantee to assure contractor's performance did not contain an arbitration clause. Nova Hut drew on the standby for the Contractor's failure to deliver contractual obligations. Against the Kaiser's action under US Bankruptcy law, Nova Hut moved to stay the proceedings pending arbitration, to compel arbitration, and to dismiss the complaint. The US Bankruptcy Court for the District of Delaware denied Nova Hut's motions. On appeal, Kaiser argued that it was not subject to arbitration since it was not a party to the contract. It also argued that Nova Hut had waived its right to arbitration by filing a proof of claim in the bankruptcy proceeding and commencing legal actions in other countries. The appeals court noted that in order to avoid arbitration on those grounds prejudice must be shown. It indicated that because there was no long delay in requesting arbitration and no discovery conducted m the course of litigation, the Kaiser could not demonstrate actual prejudice on the part of Owner. In light of public policy favoring arbitration, the nature of the claims in the parties' agreements, Kaiser's conduct in embracing the agreements, and their expectation of benefit, the appeals court ruled that the doctrine of equitable estoppel applied in requiring the Parent to arbitrate.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2018.06a
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• pp.141-141
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• 2018

Copper is one of the most abundant metals on earth. Its oxide (CuO) is an intrinsically p-type metal-oxide semiconductor with a bandgap ($E_g$) of 1.2-2.0 eV 1. Copper oxide nanomaterials are considered as promising materials for a wide range of applications e.g., lithium ion batteries, dye-sensitized solar cells, photocatalytic hydrogen production, photodetectors, and biogas sensors 2-7. Recently, high-density and uniform CuO nanostructures have been grown on Cu foils in alkaline solutions 3. In 2011, T. Soejima et al. proposed a facile process for the oxidation synthesis of CuO nanobelt arrays using $NH_3-H_2O_2$ aqueous solution 8. In 2017, G. Kaur et al. synthesized CuO nanostructures by treating Cu foils in $NH_4OH$ at room temperature for different treatment times 9. The surface treatment of Cu in alkaline aqueous solutions is a potential method for the mass fabrication of CuO nanostructures with high uniformity and density. It is interesting to compare the gas sensing properties among CuO nanomaterials synthesized by this approach and by others. Nevertheless, none of above studies investigated the gas sensing properties of as-synthesized CuO nanomaterials. In this study, CuO nanowalls versus nanoparticles were synthesized via the oxidation process of Cu foil in NH4OH solution at $50-70^{\circ}C$. The gas sensing properties of the as-prepared CuO nanoplates were examined with $C_2H_5OH$, $CH_3COCH_3$, and $NH_3$ at $200-360^{\circ}C$.

• The Bulletin of The Korean Astronomical Society
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• v.36 no.2
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• pp.113.2-113.2
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• 2011

We observed a low-mass pre-main sequence star, HBC722 (also known as $LkH{\alpha}$ 188 G4), with Camera for QUasars in EArly uNiverse (CQUEAN) attached to 2.1 Otto Struve telescope at McDonald Observatory, USA. HBC722 is a new FU orionis-type object in the direction of NGC7000/IC5070, which produced large amplitude optical outbursts (${\delta}V$=4.7 mag over one year) for a few months and reached the peak in 2010 September. We carried out the photometric observation in SDSS r,i, and z band in 2011 April, July and August to monitor the long term decrease of its brightness. We also made continuous observation in r-band for half night in July, and whole two nights in August to investigate short term variability which could be related to the rotation of the central star or the inner circumstellar disk. In this poster, we present a preliminary result of the photometric observation for HBC722.

• Journal of Environmental Science International
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• v.15 no.10
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• pp.919-928
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• 2006

The present study applied an atmospheric flow field model in Gwangyang-Bay which can predict local sea/land breezes formed in a complex terrain lot the development of a model that can predict short term concentration of air pollution. Estimated values from the conduct of the atmospheric flow field were used to evaluate and compare with observation data of the meteorological stations in Yeosu and the Yeosu airport, and the effect of micrometeorology of surround region by the coastal area reclamation was predicted by using the estimated values, Simulation results, a nighttime is appeared plainly land breezes of the Gwangyang-bay direction according to a mountain wind that formed in the Mt. of Baekwooun, Mt. of Youngchui. Land winds is formed clockwise circulation in the north, clockwise reverse direction in the south with Gangyang-bay as the center. Compared with model and observation value, Temperature is tend to appeared some highly simulation value in the night, observation value in the daytime in two sites all, but it is veil accorded generally, the pattern of one period can know very the similarity. And also, wind speed and wind direction is some appeared the error of observation value and calculation results in crossing time of the land wind and sea land, it can see that reproducibility is generally good, is very appeared the change land wind in the nighttime, the change of sea wind in the daytime. And also, according to change of the utilization coefficient of soil before and after development with Gwangyang-Bay area as the center. Temperature after development was high $0.55\sim0.67^{\circ}C$ in the 14 hoots, also was tend to appear lowly $0.10\sim0.22^{\circ}C$ in the 02 hours, the change of u, v component is comparatively tend to reduced sea wind and land wind, it is affected ascending air current and frictional power of the earth surface according to inequality heating of the generation of earth surface.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.11
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• pp.1686-1694
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• 2019

Objective: Lignin plays a relevant role in the inhibition of cell wall (CW) structural carbohydrate degradation. Thus, obtaining accurate estimates of the lignin content in tropical plants is important in order to properly characterize the mechanism of lignin action on CW degradation. Comparing conflicting results between the different methods available for commercial use will bring insight on the subject. This way, providing data to better understand the relationship between lignin concentration and implications with tropical forage degradation. Methods: Five grass species, Brachiaria brizantha cv $Marand{\acute{u}}$, Brachiaria brizantha cv $Xara{\acute{e}}s$(MG-5), Panicum maximum cv Mombaça, Pennisetum purpureum cv Cameroon, and Pennisetum purpureum cv Napier, were harvested at five maturity stages. Acid detergent lignin (ADL), Klason lignin (KL), acetyl bromide lignin (ABL), and permanganate lignin (PerL) were measured on all species. Lignin concentration was correlated with in vitro degradability. Results: Highly significant effects for maturity, lignin method and their interaction on lignin content were observed. The ADL, KL and ABL methods had similar negative correlations with degradability. The PerL method failed to reliably estimate the degradability of tropical grasses, possibly due to interference of other substances potentially soluble in the $KMnO_4$ solution. Conclusion: ADL and KL methods use strong acid ($H_2SO_4$) and require determination of ash and N content in the lignin residues, therefore, increasing time and cost of analysis. The ABL method has no need for such corrections and is a fast and a convenient method for determination of total lignin content in plants, thus, it may be a good option for routine laboratory analysis.