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Accumulation of Heavy Metals(Cd, Cu, Zn, and Pb) in Five Tree Species in Relation to Contamination of Soil near Two Closed Zinc-Mining Sites (아연폐광산(亞鉛廢鑛山) 주변(周邊) 토양(土壤)의 중금속(重金屬) (Cd, Cu, Zn, Pb) 오염(汚染)에 따른 5개(個) 수종(樹種)의 부위별(部位別) 중금속(重金屬) 축적(蓄積))

  • Han, Sim Hee;Hyun, Jung Oh;Lee, Kyung Joon;Cho, Duck Hyun
    • Journal of Korean Society of Forest Science
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    • v.87 no.3
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    • pp.466-474
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    • 1998
  • This study was conducted to evaluate heavy metal concentrations(Cd, Cu, Z, and Pb) in the soil of two zinc mines and to correlate heavy metal contents between the soils and trees growing near the mines. Soils and leaves, stems, and roots of five tree species(Corylus heterophylla, Pinus rigida. Populus alba${\times}$glandulosa, Rhododendron mucronulatum, and Robinia pseudoacacia) were collected from Sambo Zinc Mine located in Hwasung and Gahak Zinc Mine in Kwangmyung city in Kyonggido. Soils near two zinc mines were not seriously contaminated by heavy metals, but Zn and Pb concentrations were at toxic level. The heavy metal concentration in soils decreased in the order of Zn, Pb, Cu, and Cd, and decreased with increasing distance from zinc mining sites. Among the five tree species, Populus alba ${\times}$ glandulosa showed the highest heavy metal concentrations in the tissue except for Pb. Particulars, leaves of the species contained the high concentrations of heavy metals to reach the maximum of 91ppm Zn. The roots of Corylus heterophylla contained high concentrations of Cu and Pb. The order of heavy metal concentrations in the tree species was Zn, Cu, Pb and Cd. The concentration of heavy metals in the tree tissues showed a positive correlation with that in soil in which trees are growing. The ratio of heavy metal concentration of trees to that of soils(concentration factor : CF) was highest in Zn and lowest in Pb. Populus alba${\times}$glandulosa had the highest CF value among the five tree species. It was concluded that Populus alba${\times}$glandulosa, based on the high metal uptake ability, could be used for decontaminating of heavy metals from contaminated soils, and Pinus rigida could be used to reflect the level of contamination in soils.

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The Use of Autogenous Periosteal Grafts for the Periodontal Regeneration in Mandibular Class II Furcation Defects in the Dog (성견의 2급 치근 분지부 결손에서 자가골막 이식에 의한 치주조직 재생)

  • Nam, Seung-Ji;Chung, Hyun-Ju;Kim, Young-Jun
    • Journal of Periodontal and Implant Science
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    • v.30 no.2
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    • pp.241-257
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    • 2000
  • Autogenous periosteal grafts are an attractive alternative to existing barrier membrane materials since they meet the reqiurements of an ideal material. But no histological data are available on the effectiveness of periosteal membranes in the treatment of periodontal defects. The purpose of this study was to evaluate effect of autogenous periosteal graft on periodontal regeneration histologically. Class II furcation defects were surgically created on the second, third and the fourth premolars bilaterally in the mandibules of six mongrel dogs. The experimental sites were divided into three groups according to the treatment modalities; control group - surgical debridement only; Group I- autogenous periosteal membrane placement after surgical debridement; Group II-autogenous periosteal membrane placement after surgical debridement and bone grafting. The animals were sacrificed at 2, 4 and 12 weeks after periodontal surgery and the decalcified and undecalcified specimens were prepared for histological and histometrical analysis. Clinically all treated groups healed without significant problems. Under light microscope, at 2 weeks, control group showed significant apical epithelial migration and bone remodelling only below the notch area. But for the group I, II with autogenous periosteal graft, less apical migration of epithelium appeared and large amount of osteoid tissue showed above the notch area. Grafted periosteal membrane was indiscernable at 4 weeks, so periosteal membrane might be organized to surrounding tissues. Histometrically, at 4 and 12 weeks, all the test and control groups didn't show significant change of epithelial zone but new attachment level tended to be gained in the test groups than control group. These results suggest that autogenous periosteal grafts should be a good alternative for guided tissue regeneration.

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Characterization of Arsenic Immobilization in the Myungbong Mine Tailing (명봉광산의 광미 내 비소의 고정화 특성 연구)

  • Lee, Woo-Chun;Jeong, Jong-Ok;Kim, Ju-Yong;Kim, Soon-Oh
    • Economic and Environmental Geology
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    • v.43 no.2
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    • pp.137-148
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    • 2010
  • The Myoungbong mine located in Boseong-gun, Jellanamdo consists of Au-Ag bearing quartz veins which filled the fissures of Bulguksa granitic rocks of Cretaceous. The tailings obtained from the Myungbong mine were used to investigate the effects of various processes, such as oxidation of primary sulfides and formation(alteration) of secondary and/or tertiary minerals, on arsenic immobilization in tailings. This study was conducted via both mineralogical and chemical methods. Mineralogical methods used included gravity and magnetic separation, ultrasonic cleaning, and instrumental analyses(X-ray diffractometry, energy-dispersive spectroscopy, and electron probe microanalyzer) and aqua regia extraction technique for soils was applied to determine the elemental concentrations in the tailings. Iron (oxy)hydroxides formed as a result of oxidation of tailings were identified as three specific forms. The first form filled in rims and fissures of primary pyrites. The second one precipitated and coated the surfaces of gangue minerals and the final form was altered into yukonites. Initially, large amounts of acid-generating minerals, such as pyrite and arsenopyrite, might make the rapid progress of oxidation reactions, and lots of secondary minerals including iron (oxy)hydroxides and scorodite were formed. The rate of pH decrease in tailings diminished, in addition, as the exposure time of tailings to oxidation environments was prolonged and the acid-generating minerals were depleted. Rather, it is speculated that the pH of tailings increased, as the contribution of pH neutralization reactions by calcite contained in surrounding parental rocks became larger. The stability of secondary minerals, such as scorodite, were deteriorated due to the increase in pH, and finally arsenic might be leached out. Subsequently, calcimn and arsenic ions dissociated from calcites and scorodites were locally concentrated, and yukonite could be grown tertiarily. It is confirmed that this tertiary yukonite which is one of arsenate minerals and contains arsenic in high level plays a crucial role in immobilizing arsenic in tailings. In addition to immobilization of arsenic in yukonites, the results indicate that a huge amount of iron (oxy)hydroxides formed by weathering of pyrite which is one of typical primary minerals in tailings can strongly control arsenic behavior as well. Consequently, this study elucidates that through a sequence of various processes, arsenic which was leached out as a result of weathering of primary minerals, such as arsenopyrite, and/or redissolved from secondary minerals, such as scorodite, might be immobilized by various sorption reactions including adsorption, coprecipiation, and absorption.

Responses of Root Growth Characters to Waterlogging in Soybean [Glycine max (L.) Merrill] (과습에 따른 콩 지하부 생육반응)

  • Lee, Jae-Eun;Kim, Hong-Sig;Kwon, Young-Up;Jung, Gun-Ho;Lee, Chun-Ki;Yun, Hong-Tai;Kim, Chung-Kon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.1
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    • pp.1-7
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    • 2010
  • Stress from excess water is one of the most harmful limiting factor in soybean yield during the wet season under the climate conditions. Soybean is very sensitive to excess water compare to other crops. This experiment was carried out to identify the growth responses for establishing a screening system related to waterlogging tolerance in soybean from 2003 to 2004. The root dry weight accumulation rate of per day for 21 days after waterlogging at V5 stage was the highest in Pungsannamulkong (47~56% of control) and was the lowest in Jangyeobkong (26~27% of control). The nodules dry weight recovery rate was the highest in Pungsannamulkong (83~91% of control), while the lowest in Myungjunamulkong (48~66% of control). After waterlogging, recovery rate of roots was high, which increased the root/shoot ratio of Pungsannamulkong, which also produced significantly more adventitious roots than in Jangyeobkong. The percentage of adventitious roots fresh weight to the total roots fresh weight was the lowest in Myungjunamulkong (14%), while the highest in Pungsannamulkong (38%). This results implies that the water and nutrients absorbing ability of Pungsannamulkong is more higher than that of Myungjunamulkong during late growth period.

Comparison of Nutrient Balance in a Reclaimed Tidal Upland between Chemical and Compost Fertilization for the Winter Green Barley Cultivation (간척농경지에서 비종에 따른 동계 청보리 재배 포장의 영양물질 수지 비교)

  • Song, In-Hong;Lee, Kyong-Do;Kim, Ji-Hye;Kang, Moon-Seong;Jang, Jeong-Ryeol
    • Korean Journal of Environmental Agriculture
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    • v.31 no.2
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    • pp.137-145
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    • 2012
  • BACKGROUND: Along with the surplus rice production, introduction of upland crop cultivations into newly reclaimed tidal areas has gained public attentions in terms of farming diversification and farmers income increase. However, its impacts on the surroundings have not been well studied yet, especially associated with nutrient balance from reclaimed upland cultivation. The objective of this study was to investigate water and nutrient balance during winter barley cultivation as affected different fertilization methods. METHODS AND RESULTS: TN and TP balance for three different plots treated by livestock compost, chemical fertilizer, and no application were monitored during winter green barley cultivation (2010-2011) at the NICS Kyehwa experimental field in Jeonbuk, Korea. Nutrient content in soil and pore water near soil surface appeared to increase, while sub-soil layer remained similar with no fertilization plot. Livestock compost application appeared to increase organic matter content in surface soil compared to chemical fertilization. Crop yield was the greatest with livestock compost application (10.6 t/ha) followed by chemical fertilization (6.9 t/ha) and no application (1.8 t/ha). The nitrogen uptake rate was also greater with livestock compost (52.4%) than chemical fertilizer (48.1%). Phosphorus uptake rate was much smaller (about 7.0%) compared to nitrogen. Nutrient loss by surface and subsurface runoff seemed to be minimal primarily due to small rainfall amount during the winter season. Most of the remaining nutrients, particularly phosphate seemed to be stored in soil layer. Phosphate accumulation appeared to be more phenomenal in the plot applied by livestock compost with higher phosphorus content. CONCLUSION: This study demonstrated that livestock compost application to tidal upland may increase barley crop production and also improve soil fertility by supplying organic content. However, excessive phosphorus supply with livestock compost seems likely to cause a phosphate accumulation problem, unless the nitrogen-based fertilization practice is adjusted.

Screening of Adsorbent to Reduce Salt Concentration in the Plastic Film House Soil under Continuous Vegetable Cultivation (시설채소재배지의 토양특성과 흡착제 종류별 염류경감 효과)

  • Ok, Yong-Sik;Yoo, Kyung-Yoal;Kim, Yoo-Bum;Chung, Doug-Young;Park, Yong-Ha;Yang, Jae-E.
    • Korean Journal of Environmental Agriculture
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    • v.24 no.3
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    • pp.253-260
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    • 2005
  • Salt accumulation in the plastic film house soils under continuous cultivation condition causes problems such as salt damages to plants, nitrate accumulation in vegetables, groundwater contamination, etc. due to excess application of fertilizers. Objective of this research was to find an optimum adsorbent to reduce salt concentration in the soil solution of plastic film house soils, where crop injuries have been observed due to the salt accumulation. The soils were significantly high in available P $(1,431{\sim}6,516mg\;kg^{-1}),\;NO_3-N\;(117.60{\sim}395.73mg\;kg^{-1})$, exchangeable Ca $(4.06{\sim}11.07\;cmol_c\;kg^{-1})$ and Mg $(2.59{\sim}18.76\;cmol_c\;kg^{-1})$, as compared to those of the average upland soils in Korea. Soils were treated with each of adsorbent such as ion-exchange resin, zeolite, rice bran, etc. at 2% level and prepared into saturated-paste samples. After equilibrium, soil solution was vacuum-extracted from the soil and measured for changes of the pH, EC, and concentrations of $Ca^{2+},\;Mg^{2+},\;K^+,\;Na^+,\;{NH_4}^+,\;{PO_4}^{3-}\;and\;{NO_3}^-$. Rice bran effectively removed ${PO_4}^{3-}\;and\;{NO_3}^-$ in the soil solution up to 100%. Efficiency was decreased in the orders of rice bran > ion-exchange resin > zeolite. Removal efficiencies of zeolite and ion-exchange resin for $Ca^{2+}$ were ranged from 1 to 65% and from 7 to 61%, respectively. Ion-exchange resin was also effective for removing $Mg^{2+},\;K^+,\;Na^+,\;and\;{NH_4}^+$. Overall results demonstrated that rice bran and ion-exchange resin could be applicable for salt accumulated soil to remove the respective anion and cation.

Studies on the Nutri-Culture of Major Wild Vegetable Ligularia fischeri Turcz I. Growth and Yield of Ligularia fischeri Turcz. by Media, Amount of Media and Planting Density in Nutri-Culture (곰취의 양액재배 기술 개발 I. 배지종류 , 배지량 , 재식밀도에 따른 곰취의 생육 및 수량)

  • 홍정기
    • Korean Journal of Plant Resources
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    • v.10 no.4
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    • pp.401-410
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    • 1997
  • This experiment was conducted to investigate the opimum media, media amount and planting density of nutri-culture for the high quality and yield of wild vegetable, Liguliaria fischeri Turez. The fried rice hull was recognized best media among perlite, sand and fried rice hull for nutri-culture of Ligularia fischeri Turcz. The yield on fried rice hull media at early growing stage was lower, but higher than in other media at late growing stage. Therefore, total yield in fried rice hull media was 15-29% higher than that of the existing perlite media. Growth and yield of Ligularia fischeri Turcz. were not significantly different between medium amounts, therefore considering economical efficiency and stability, the optimum medium amount was 105 $\iota/m^3$, Yield by planting density increased in the high density and 50 plants/$m^2$ was considered as proper planting density in nutri-culture of Liguliaria fishcheri Turcz.

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Evaluation of Countermeasures Effectiveness in a Radioactively Contaminated Urban Area Using METRO-K : The Implementation of Scenarios Designed by the EMRAS II Urban Areas Working Group (METRO-K를 사용한 방사능으로 오염된 도시지역에서 대응행위효과 평가 : EMRAS II 도시오염평가분과 시나리오의 이행)

  • Hwang, Won-Tae;Jeong, Hae-Sun;Jeong, Hyo-Joon;Kim, Eun-Han;Han, Moon-Hee
    • Journal of Radiation Protection and Research
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    • v.37 no.3
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    • pp.108-115
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    • 2012
  • The Urban Areas Working Group within the EMRAS-2 ($\underline{E}$nvironmental $\underline{M}$odelling for $\underline{RA}$diation $\underline{S}$afety, Phase 2), which has been supported by the IAEA (International Atomic Energy Agency), has designed some types of accidental scenarios to test and improve the capabilities of models used for evaluation of radioactive contamination in urban areas. For the comparison of the results predicted from the different models, the absorbed doses in air were analyzed as a function of time following the accident with consideration of countermeasures to be taken. Two kinds of considerations were performed to find the dependency of the predicted results. One is the 'accidental season', i.e. summer and winter, in which an event of radioactive contamination takes place in a specified urban area. Likewise, the 'rainfall intensity' on the day of an event was also considered with the option of 1) no rain, 2) light rain, and 3) heavy rain. The results predicted using a domestic model of METRO-K have been submitted to the Urban Areas Working Group for the intercomparison with those of other models. In this study, as a part of these results using METRO-K, the countermeasures effectiveness in terms of dose reduction was analyzed and presented for the ground floor of a 24-story business building in a specified urban area. As a result, it was found that the countermeasures effectiveness is distinctly dependent on the rainfall intensity on the day of an event, and season when an event takes place. It is related to the different deposition amount of the radionuclides to the surfaces and different behavior on the surfaces following a deposition, and different effectiveness from countermeasures. In conclusion, a selection of appropriate countermeasures with consideration of various environmental conditions may be important to minimize and optimize the socio-economic costs as well as radiation-induced health detriments.

Effect of Fluoride Recharging on Fluoride Release and Surface Properties of Orthodontic Bracket Adhesives (불소 적용 후 교정용 브라켓 접착제 종류에 따른 불소 재흡수성과 표면 변화에 관한 연구)

  • Byeon, Seon Mi
    • Journal of dental hygiene science
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    • v.18 no.4
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    • pp.218-226
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    • 2018
  • The aim of this study was to compare fluoride release and surface changes according to different orthodontic bracket adhesives the application of fluoride products. We used non-fluoridated composite resin Transbond fluoridated composite resins Blugloo and LightBond, resin-modified glass ionomer Rely $X^{TM}$ Luting 2, and conventional glass ionomer Fuji $I^{(R)}$. Fluoride release of five orthodontic bracket adhesives and fluoride release ability after application of three fluoride products (1.23% acidulated phosphate fluoride gel, Tooth Mousse $Plus^{(R)}$, Fluor Protector, and a toothbrush with sodium fluoride-containing toothpaste) were measured using a fluoride electrode that was connected to an ion analyzer. After 4 weeks of fluoride application, the surface roughness and surface morphology were examined using a surface roughness tester and field emission scanning electron microscopy. The amounts of fluoride release were observed not only on application of Tooth Mousse $Plus^{(R)}$ and Fluor Protector on resin-modified glass ionomer Rely $X^{TM}$ Luting 2 and Fuji $I^{(R)}$, but also during tooth brushing using fluoride-containing toothpaste. After application of Tooth Mousse $Plus^{(R)}$, except Transbond XT, the surface roughness increased, and all orthodontic adhesives showed a partial drop of micro-particle filler. On application of 1.23% acidulated phosphate fluoride gel on all orthodontic bracket adhesives, their surface roughness increased. To bond the orthodontic bracket, resin-modified glass ionomer Rely $X^{TM}$ Luting 2 and Fuji $I^{(R)}$ adhesives are highly recommended if the amount of fluoride release is considered to confer a preventative effect on dental caries, and among the fluoride products, Tooth Mousse $Plus^{(R)}$ and Fluor Protector are better than 1.23% acidulated phosphate fluoride gel, and these are expected to prevent dental caries even during tooth brushing with fluoride-containing toothpaste.

Antioxidative and Antibacterial Activities of Artemisia princeps Pampanini Extracts (사자발쑥 추출물의 항산화 및 항균 활성)

  • Yang, Hyun Gab;Kim, Hye Jin;Kim, Hae Soo;Park, Soo Nam
    • Microbiology and Biotechnology Letters
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    • v.40 no.3
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    • pp.250-260
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    • 2012
  • In the present study, the antioxidative and antibacterial activities of Artemisia princeps Pampanini (A. princeps Pamp.) extract were investigated. The ethyl acetate fraction of A. princeps Pamp. showed the most prominent free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity ($FSC_{50}=12.27{\mu}g/mL$). Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of A. princeps Pamp. extract on $Fe^{3+}-EDTA/H_2O_2$ systems were investigated using a luminol-dependent chemiluminescence assay. The ethyl acetate fraction of the extract ($OSC_{50}=0.33{\mu}g/mL$) had a 5 times greater ROS scavenging activity than L-ascorbic acid ($1.50{\mu}g/mL$), known as a water soluble antioxidant. The cellular protective effects of fractions of A. princeps Pamp. on the rose-bengal sensitized photohemolysis of human erythrocytes were examined. The aglycone fraction of extracts suppressed photohemolysis in a concentration dependent manner. The inhibitory effects of A. princeps Pamp. extract on tyrosinase were investigated to assess their whitening efficiency. The ethyl acetate fraction demonstrated a 7 times higher tyrosinase inhibitory effect ($IC_{50}=29.20{\mu}g/mL$) than albutin, known as a whitening agent. The antibacterial activity of ethyl acetate fractions against various normal skin flora were measured. The results showed that the antibacterial activity of the fraction was the highest on Staphylococcus aureus, Bacillus subtilis, and Propionibacterium acnes. Antioxidant substances were isolated and purified from the ethyl acetate fractions. Eupatilin and jaceosidin were identified. These results indicate that the extract/fractions of A. princeps Pamp. can function as antioxidant and/or antibacterial agents for the skin.