• Journal of Pharmaceutical Investigation
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• v.38 no.6
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• pp.399-403
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• 2008

The aim of this study was to evaluate the difference of analytical methods for the pharmacokinetic study of bromosulfophthalein (BSP), an indicator of hepatobiliary function. The plasma and bile concentrations of BSP after intravenous administration were measured according to custom UV spectroscopy and HPLC, respectively. Plasma concentration of BSP measured by UV spectroscopy was similar to that measured by HPLC. There was no significant difference in the distribution volume, total body clearance, area under the curve and mean residence time of BSP between different analytical method groups. However, bile concentration of BSP measured by UV spectroscopy was overestimated compared with concentration measured by HPLC method. Biliary clearance of BSP obtained from UV spectroscopy method was almost 3 times higher than that obtained from HPLC method. Thus, a feasibility of UV spectroscopy method for high throughput pharmacokinetic evaluation of BSP was limited to the study based on the plasma concentration of BSP, not bile concentration.

• Journal of environmental and Sanitary engineering
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• v.11 no.1
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• pp.1-8
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• 1996

In the present study the sesamin and sesamol contents of the sesame extracts prepared from nine provinces in Korea were determined HPLC method. A comparative test was also carried out using the Villavecchia-suarez test, the red colored reaction for the sesamol and sesamol derivatives. The contents of sesamin and sesamol of the sesame oils from each area by the HPLC method were 0.57-0.78% and 0.010-0.023%, respectively, and the paralled results were obtained by the Villavecchia-suarez test and the HPLC method. The average content of the sesamin was $0.68{\pm}0.074%$ by the HPLC method and the average absorbance of the Villavecchia-suarez test was $0.56{\pm}0.034$. The contents of sesamol from sesame oil by the HPLC method and the Villavecchia-suarez test were so low that it was not possible to correlate with the sesamin contents. The contents of sesamol from the sesame oil produced in Kyeong-gi and Jeon-nam provinces were $0.010{\pm}0.002%$ and $0.023{\pm}0.004%$, respectively.

• Journal of the Korean Chemical Society
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• v.31 no.1
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• pp.45-54
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• 1987

Dowex 1-X8 resin ion exchanged with calcon carboxylic acid (CCA-Dowex 1-X8) and 2-methyl-8-hydroxyquinoline(MHQ) impregnated-Amberlite XAD-4 resin (MHQ-XAD-4) were examined for the separation and preconcentration of ferric ion from the various matrices. The stabilities of these resins were investigated, and their capacities on ferric ion were also measured. The effect of pH on the adsorption of ferric ion and matrix ions, such as Al(Ⅲ) and Ca(Ⅱ), was investigated to determine the optimun pH ranges. Separation and preconcentration of iron in aluminium foil and mineral water samples were studied by elution method with these resin columns. The recovered ions by 10ml of 2F nitric acid was determined by flame atomic spectrophotometry. SP-Sephadex C-25 column was used to separate ferrous and ferric ion in mineral water by stepwise elution with ferrozine and 1 % ascorbic acid-ferrozine solution. The concentrated and separated each ion could be determined spectrophotometrically at the analytical wavelenth of Fe(Ⅱ)-ferrozine complex (562nm).

• Journal of Environmental Health Sciences
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• v.45 no.3
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• pp.258-266
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• 2019

Objectives: In this study, blood lead was analyzed using graphite furnace atomic absorption spectrometry (GF-AAs) and inductively coupled plasma mass spectrometry (ICP-MS). We tried to examine the difference and consistency of the analytical values and the applicability of the analytical method. Methods: We selected 57 people who agreed to participate in this study. After confirming the linearity of the calibration standard curves in GF-AAs and ICP-MS, the concentrations of lead in quality control material and samples were measured, and the degree of agreement was compared. Results: The detection limit of the ICP-MS was lower than that of GF-AAs. The coefficient of variation of reference materials was shown to be stable in the ICP-MS and GF-AAs. When the correspondence between the two equipments was verified by bias of the analysis values, a concordance was shown, and approximately 98% of the ideal reference lines were present within ${\pm}40%$ of the deflection. Conclusion: GF-AAs showed high sensitivity to single heavy metal analysis, but it took much time and showed higher detection limit than ICP-MS. Therefore, it would be considered necessary to switch to ICP-MS analysis method, considering that the level of lead exposure is gradually decreasing.

• Korean Journal of Ecology and Environment
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• v.39 no.2 s.116
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• pp.257-264
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• 2006

In order to evaluate the application of Algae Online Analyzer (AOA), an instrument of automatic measurement of chlorophyll a concentration, was tested and compared with the acetone extraction method on the basis of microscopic counting of phytoplankton in field water (Paltang Reservoir). We simultaneously conducted AOA operation and extraction method with the same water sample, to compare both results of chlorophyll a measurement. Phytoplankton were enumerated by inverted microscope with the Sedgwick-Rafter chamber, and classified into the genus or species. According to the AOA measurement, the diatom most (83.6%) strongly contributed to the total chlorophyll a concentration, followed by chlorophyceae> cyanophyceae>cryptophyceae. Overall, the results of both AOA and extraction method showed a similar trend and significant correlation (r=0.87, n=302, p<0.001), however, there were some differences according to the season and species. In particular, the relationship between AOA Chl-a density of the diatom (r=0.73, p=0.010) and cyrptophyceae (.=0.83, p=0.00154) were siginificant, while chlorophyceae (r= -0.13) and cyanophyceae (r= -0.16) showed no clear relationship during the study period. Although we can not fully understand why there was difference between both mothods, AOA application for alarming algal bloom and water quality management during the algal bloom appears to be very relevant. However, the further study or technical upgrade of AOA measurement is required, especially in the case of low density of phytoplankton or species-specific measurement.

• Journal of Korean Society of Water and Wastewater
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• v.27 no.6
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• pp.827-836
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• 2013

Due to the stringent drinking water quality, formaldehyde will be included in Korean drinking water standard from year 2014. However, its standard analytical method has not yet been established. This study compares two analytical methods, DNPH-LC and ACFAS with respect to their analysis principles, Method Detection Limit (MDL), Limit Of Quantitation(LOQ), precision, accuracy, reproducibility, convenience, number of samples analyzed per hour and analysis cost. These methods measure absorption intensity at 360 nm by using HPLC after DNPH-derivatization (DNPH-LC) and at 410 nm by using Automated Continuous Flow Absorption Spectrophotometer (ACFAS), respectively. Reproducibility was tested by repeating the analysis 7 times using a standard solution for each method. For DNPH-LC method, MDL was $0.5{\mu}g/L$, LOQ was $1.58{\mu}g/L$ with standard deviation of $0.16{\mu}g/L$. For ACFAS method, they were $0.27{\mu}g/L$, $0.85{\mu}g/L$L with standard deviation of $0.09{\mu}g/L$, respectively. Both methods satisfied the requirement set by the Korean drinking water quality standard. Complexity of sample pretreatment procedure for DNPH-LC method may cause large error and, consequently, the analytical result will depend on the level of skill of analyst. In contrast, ACFAS method which used only one reagent equipped with an automated injection device showed little analytical error. It costs about $5.00 and $1.00 for one sample to analyze by the DNPH-LC method and the ACFAS method, respectively. Compared to the DNPH-LC method, ACFAS method provided more reliable analytical results. In terms of convenience, easiness and analytical cost, ACFAS method was demonstrated to be superior to the DNPH-LC method. The results of this study suggested that the ACFAS method could be adapted as a proper method for determining formaldehyde content in drinking water.

• Journal of Soil and Groundwater Environment
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• v.7 no.3
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• pp.71-77
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• 2002

This research was carried out from 2001. 4 until 2001. 5 in order to examine the level of soil contamination in the vicinity of Nanjido. The sampling was done at near the Nanjido soil. At the each sampling site we did the sampling at different depth- surface(0~15cm deep), 1m deep, 2m deep, 3m deep. The contamination of Cd, Cu, Pb, As, $Cr^{+6}$, and Hg were tasted and analysed by spectra AA. The examination showed that the average concentration level of the entire sampled soil was Cd, 0.229mg/kg, Cu 8.349mg/kg, Pb : 11.083mg/kg, As : 0.298mg/kg, $Cr^{+6}$ : 0.124mg/kg, and Hg : 0.134mg/kg. The concentration level of at the depth of 0-l5cm, came out to be Cd : 0.305mg/kg, Cu : 8.464mg/kg, Pb : 11 383mg/kg, As : 0.128mg/kg, $Cr^{+6}$ : 0.153mg/kg, and Hg : 0.092mg/kg. It shows that in the cases of Cd and C $r^{+6}$ the average concentration level of the whole sampled soil was about 80% of that of 0-l5cm depth. And as for Hg and As. the average concentration level of the entire sampled soil came out to be approximately twice as high as the sample soil from the depth surface, As for Cu and Pb, there was not much difference between the entire samples average concentration level and the concentration level of surface. It was hard to find much relationship between the depth of each site where sampling was done and the level of concentration, the concentration level of Pb and Cu at the sampling site C and E was quite high, which suggests that it has been affected by the polluted the hyang-dong stream and fill random up waste.

• Journal of Food Hygiene and Safety
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• v.2 no.3
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• pp.103-108
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• 1987

ABSTRACT-The study was to investigate the residue of the harmful heavy metals in the random samples of feed from piggery, formula feeed for swine and ingredient feed from feed mill and analyzed for Cd, Pb and As by using Atomic Absorption Spectrophotometer and Inductively Coupled Argon Plasmas. Mean concentrations of Cd, Pb and As (ppm) were, for feed from piggery 1.08-0.85, 5.34-4.29 and 4.30-2.37, for formula feed from feed mill, 0.90-0.51, 5.84-5.25 and 2.41-0.84, and for ingredient feed from feed mill, 0.35-0.38, 4.38-4.94 and 2.66-1.12, respectively. It showed the highest amount of Cd, Pb and As in kidney and liver than in the other organs, and the lowest amount in the muscle.muscle.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.6 no.1
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• pp.77-87
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• 1996

In order to compare the difference of the measurement of delta aminolevulinic acid(${\delta}$-ALA) in urine between HPLC method(HALA) and colorimetric method(CALA), and also to provide useful information for the new diagnostic criteria of ${\delta}$-ALA in urine in lead poisoning, if at all possible in the future, authors studied 234 male lead workers who were selected from 7 storage battery factories, 3 secondary smelting industries, and 2 litharge making industries. Study subjects were selected on the basis of blood Zinc protoporphyrin(ZPP) level from low to high concentration to cover wide range of lead exposure. Study variables for this study were ${\delta}$-ALA measured by two different methods, blood lead(PbB), and blood ZPP. The results were as follows: 1. There was very high correlation between ${\delta}$-ALA measured by two method(r = 0.989 : HALA = -0.8194 + 0.8110 ${\times}$ CALA), but the value of CALA was measured about 2mg/L greater than HALA. 2. While the correlations of ${\delta}$-ALA by two method with blood lead and blood ZPP were 0.46 and 0.37 respectively, they were increased to 0.63 and 0.57 if ${\delta}$-ALA values were log-transformed. 3. Simple linear regression of ${\delta}$-ALA measured by two method on ZPP were as follows: CALA = 2.0421 + 0.0341 ${\times}$ ZPP ($R^2=0.1385$ p = 0.0001) HALA = 0.8006 + 0.0280 ${\times}$ ZPP ($R^2=0.1389$ p = 0.0001) 4. Simple linear regression of ${\delta}$-ALA measured by two method on PbB were as follows: CALA = - 0.4134 + 0.1545 ${\times}$ PbB ($R^2=0.2085$ p = 0.0001) HALA = -1.2893 + 0.1287 PbB ($R^2=0.2154$ p = 0.0001), 5. Simple linear regression of log-transformed ${\delta}$-ALA by two method on ZPP and PbB were as follows: logHALA = 0.3078 + 0.0060 ZPP ($R^2=0.3329$ p = 0.0001) logCALA = 1.0189 + 0.0044 ZPP ($R^2=0.3290$ p = 0.0001) logHALA = -0.0221 + 0.0246 PbB ($R^2=0.4046$ p = 0.0001) logCALA = 0.7662 + 0.0184 PbB ($R^2=0.4108$ p = 0.0001) 6. The cumulative percent of colorimetric method to detect lead workers whose value of PbS and ZPP were over screening level such as $40{\mu}/dl$ and $100{\mu}/dl$ respectively was higher than HPLC method if cut-off level of ${\delta}$-ALA for screening of lead poisoning was 5 mg/L. But if cut-off level of ${\delta}$-ALA measured by HPLC was reduced to 3 mg/L which is compatible to 5 mg/L of ${\delta}$-ALA measured by colorimetric method, there were good agreement between two methods and showed dose-response relationship with other lead exposure indices such as PbB and ZPP.

• Korean Journal of Food Science and Technology
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• v.39 no.4
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• pp.372-379
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• 2007

A high-performance liquid chromatography-electrospray ionization (HPLC-ESI) tandem MS was developed for the rapid and simultaneous determination of forbidden medicines in dietary supplements. Thirteen medicinal components such as PDE-5 inhibitors and their analogues, and the newly identified dimethylsildenafil and xanthoanthrafil, were included in this study. After tentative standardization of molecular ions in both polarities using thirteen references on the mass spectrometer, with ESI-continuous infusion via the syringe pump method, the relative intensity of the ions present in the resulting spectra was quantitatively compared. From the results, the ion mode was selected depending on each reference's characteristics. A HPLC method coupled with the ESI mode was developed considering the matrix effect and interference depending on the type of sample. The validation test of the developed method was followed by carrying out precision, accuracy, recovery, sensitivity and linearity, etc. The method showed sufficiently high sensitivity, reproducibility, and specificity, and produced 4 times faster results when compared with the existing HPLC/UV method for the determination of forbidden compounds in dietary supplements.