• Environmental Mutagens and Carcinogens
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• v.22 no.3
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• pp.199-204
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• 2002

It has been known that Ganoderma lucidum and Lentinus edodes have anticancer activity and immune enhancing activity. These two mushrooms were grown in liquid culture and harvested. From these mycelia, DNA was isolated and EtBr-CsCl density gradient ultracentrifugation was performed to purify it further. Then mitochondrial DNA was isolated by bisbenzimide-CsCl density ultracentrifugaton. Mitochondrial DNA of Ganoderma lucidum was digested by restriction enzymes, EcoR I, Hind Ⅲ, and Pst I, then electrophoresed. It showed 12, 22, 4 fragments. Mitochondrial DNA of Lentinus edodes was digested by EcoR I. Electric pattern showed 6 fragments. 4 fragments had appeared by Pst 1 digested mitochondrial DNA. Hind ill couldn't digest mitochondrial DNA of Lentinus edodes. Mitochondrial DNA of fusants was isolated to compare to those of parents. The results showed that fusant P₂S₄has new, recombined mitochondrial DNA. But P₂S₄had the same DNA that Ganoderma lucidum had.

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2002.11a
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• pp.145-153
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• 2002

유해 화학 물질류에 의해 오염된 해양 환경 시료의 환경독성 수준을 평가하기 위하여 다양한 화학물질에 대해 민감성이 우수한 생물학적 독성평가기법을 개발 하고자하였다. 지속성 유기오염 물질 중 다환 방향 족 탄화수소(PAHs)를 처리한 광어(Conger myriaster)와 개조개(Saxidomus pupurata)의 DNA 손상정도를 single cell gel electrophoresis assay(comet assay)를 통해 분석하였다. PAHs 중 광양만에서 높은 농도로 검출되는 benzo(a)pyrene을 농도별(0, 10, 50, 100 ppb)로 처리한 후 2일과 4일에 광어의 혈액세포와 개조개의 근육세포를 채취해 comet assay를 실시하였다. benso(a)pyrene에 대한 DNA 손상정도를 처리된 농도와 생물종에 따라 다르게 나타났는데 광어의 혈액세포는 2일에 가장 DNA 손상정도가 높았고, 4일에는 회복되는 경향을 나타냈다. 개조개의 근육세포는 시간이 지나면서 DNA 손상정도가 증가하는 경향을 보였다. 이와 같은 결과는 comet assay 기법이 유해 화학물질로 오염된 해양생물 종의 환경독성을 검색하는 유용한 수단이 될 수 있음을 보여준다.

• Environmental Mutagens and Carcinogens
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• v.19 no.2
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• pp.112-115
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• 1999

The action mechanism of the inhibitory effect of some natural products on the DNA strand break and DNA damage was investigated in vitro and in vivo. In the E. coli chromosomal DNA strand break experiment in vitro, three mushroom water extracts were effective on the DNA strand breaking by daunorubicin. Phellinus linteus water extract inactivated daunorubicin, a DNA strand breaking agent, but did not protect DNA from daunorubicin-induced DNA strand breaking. Agaricus blazei water extract inhibited DNA strand breaking action of daunorubicin not only by daunorubicin inactivation, but also by DNA protection from daunorubicin. An inhibitory effect of Ganoderma lucidum water extract on the DNA strand break was based on the DNA protection rather than daunorubicin inactivation. In vivo mutagen assay system (SOS-chromotest), among three mushroom water extracts Phellinus linteus water extract was the most effective one on the inhibition of DNA damage by 4-NQO. The results suggest that all three mushroom water extracts inhibit daunorubicin-induced DNA damage and in vivo DNA damaging action of 4-NQO by the reaction of mutagen inactivation or DNA protection from the mutagen.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 1996.11a
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• pp.118-121
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• 1996

환경이 나이어린 학문 정도로 생각하는 것은 환경분야의 눈부신 발전과 연구를 모르는 데서 비롯된 오해이다. 산업화에 따른 산업재앙의 위기에. 처한 환경 선진국들은 이미 막대한 재정지원을 통해서 이미 오래전부터 최신 기법들을 이용한 실험들을 성공리에 마치고 있고 또 실제 생활에 활용하고 있다. 특히 환경오염에 대한 처리방법에 있어서 미국을 비롯한 선진국들은 유전공학을 이용한 최신기법들을 연구개발하고 있으며 이것은 매우 놀랍고 고무적인 일이다. 그런 의미에서, 이 글은 환경오염의 생물학적 처리복구기술에 있어서 DNA probe(DNA 탐침자)의 역할과 이 연구를 통한 미래환경발전의 전망에 대해 살펴보고자 한다.

• Korean Journal of Environmental Biology
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• v.19 no.1
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• pp.19-24
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• 2001

Agricultural pesticides may cause certain biological risks since they are widely used to eradicate pests. Agricultural disasters may arise even from the possibility of their synergistic interaction with other harmful enviromnetal factors. The effect of pesticide on radiation-induced DNA damage in human blood lymphocytes was evaluated by the single cell gel electrophoresis (SCGE) assay. The lymphocytes, with or without pretreatment of the pesticide, were exposed to 0-2.0 Gy of $^60 CO$ gamma ray. Significantly increased tail moment, which was a marker of DNA strand breaks in SCGE assay, showed an excellent dose-response relationship. The present study confirms that the pesticide has the cytotoxic effect on lymphocytes and that it shows the synergistic interaction with radiation on DNA damage as well. The results may have a role of providing biological information necessary for the prevention of environmental disaster.

• Environmental Mutagens and Carcinogens
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• v.9 no.2
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• pp.63-72
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• 1989

본 연구는 N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) 를 처리한 CHO-K1세포에서 DNA 복제억제와 그 회복과정의 분자론적 기작을 규명할 목적으로 방사선 이중표지에 의한 DNA합성율의 측정, 알카리 자당농도구배 초원심분리법에 의한 DNA 분자량과 후복제 회복율을 측정하여 다음과 같은 결과를 얻었다. DNA 합성율은 2nM 이하의 낮은 농도의 MNNG 처리군에서는 급격히 감소하였으나, 5nM 이상의 농도에서는 그 감소양상이 둔화되었다. 억제되었던 DNA 합성율은 시간경과에 따라 회복되어 처리 후 4시간 째에는 대조군 수준 또는 그 이상으로 회복되었다. MNNG 처리 후 DNA 분자 크기의 분포와 새로 합성된 DNA 분자의 생장양상을 알카리 자당농도구배 초원심분리법으로 조사한 결과 MNNG 처리 후 시간 경과에 따라 새로합성된 DNA 분자들의 크기분포는 1*107 달톤 이하의 DNA 분자들의 합성양이 특이하게 증가하였다가 감소함을 보였다.

• Korean Journal of Environmental Biology
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• v.18 no.1
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• pp.53-61
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• 2000

In order to characterize the genetic diversity of bacterial community in groundwater, samples were collected from used for drinking water and polluted with heavy metal wastewater in Seoul city and natural cave of Kangwondo. The DNA was amplified with 165 rDNA-based primers by use of the PCR, and then analysed ARDRA (amplified ribosomal DNA restriction analysis). Restriction endonuclease analysis patterns of amplified 165 rDNA in drinking water and wastewater relatively showed high genetic diversity in situ and drinking groundwater. The number of DNA fragments varied with in situ and drinking water. This method of ARDRA of bacterial communities in groundwater could be used for a quick assessment of genotypic changes between different locations reflecting different environmental conditions and the diversity reflected pollution of groundwater (natural cave water>drinking water>waste water, as in order of grade). [Genetic diversity, Groundwater, 165 rDNA, PCR, ARDRA].

• Environmental Mutagens and Carcinogens
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• v.8 no.1
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• pp.1-12
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• 1988

The effects of aphidicolin (APC), an inhibitor of DNA polymerase alpha, or 2', 3'-dideoxythymidine 5'-triphosphate (ddTTP), an inhibitor of DNA polymerase beta, on the repair of DNA damage induced by ethyl methanesulfonate (EMS) or bleomycin (BLM) were investigated in Chinese hamster ovary (CHO)-K1 cells. Three assays were employed in this study: unscheduled DNA synthesis, alkaline elution and alkaline sucrose gradient sedimentation. It was shown that APC or ddTTP inhibited DNA induced by EMS, and thus, the post-treatment with APC or ddTTP following EMS treatment was resulted in the more amount of unscheduled DNA synthesis, and the more accumulation of DNA single-stand breaks than the cells post-incubated without APC or ddTTP. While, in the BLM induced DNA repair, only ddTTP inhibited DNA repair induced by BLM. And thus, the groups post-incubated with or without APC after BLM treatment had the same value in the amount of unscheduled DNA synthesis and of DNA single-strand breaks, while post-treatment with ddTTP was resulted in the increased amount of unscheduled DNA synthesis and the increased DNA sin -strand breaks than the group without ddTTP. These results suggested that both of DNA polymerase $\alpha$ and $\beta$ participated in the repair of DNA damage induced by EMS, but in BLM-induced DNA repair, polymerase $\beta$ participated.ipated.

• Journal of Korean Society on Water Environment
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• v.24 no.6
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• pp.817-822
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• 2008

In order to identify various Cryptosporidium species in environment, nested PCR-RFLP and DNA sequencing method were used. The sensitivity of nested PCR-RFLP based on 18s rRNA gene was shown to 1 oocyst. Therefore, we applied nested PCR-RFLP method to environmental samples. As a result, only 4 samples out of 8 samples confirmed as Cryptosporidium parvum by standard method of Cryptosporidium were identified as Cryptosporidium parvum by nested PCR-RFLP and DNA sequencing method. The rest of 4 samples among 8 samples were identified as Cryptosporidium muris, Cryptosporidium bailey. Therefore, in addition to standard method of Cryptosporidium, supplementary verification through nested PCR-RFLP and DNA sequencing should be needed to give more accurate information about risk of Cryptosporidium.

• Korean Journal of Environmental Biology
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• v.17 no.3
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• pp.279-284
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• 1999

To investigate soil bacterial diversity according to vegetation types, directly extracted DNA from 5 different soils were cross-hybridized with each other as a probe and target. Pinus densiflora soil was shown the highest value then agricultured soil＞naked soil＞grass soil＞Quercus mongolicas soil in the order of diversity. Cluster analysis by similarity showed that soil microbial communities were categorized into three groups.