Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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The Diversity and Similarity of Soil Microbial Communities by DNA Cross Hybrization

DNA 교잡에 의한 토양 미생물 군집의 다양성과 유사성

  • 김유영 (한양대학교 생물학과, 서울대학교 분자미생물학연구센터) ;
  • 송인근 (한양대학교 생물학과, 서울대학교 분자미생물학연구센터) ;
  • 민병례 (상명대학교 생물학과) ;
  • 조홍범 (서경대학교 생물공학과) ;
  • 최영길 (한양대학교 생물학과, 서울대학교 분자미생물학연구센터)
  • Published : 1999.09.01
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Abstract

To investigate soil bacterial diversity according to vegetation types, directly extracted DNA from 5 different soils were cross-hybridized with each other as a probe and target. Pinus densiflora soil was shown the highest value then agricultured soil＞naked soil＞grass soil＞Quercus mongolicas soil in the order of diversity. Cluster analysis by similarity showed that soil microbial communities were categorized into three groups.

토양으로부터 직접 추출한 DNA를 cross hybridization하는 방법을 통해서 서로 다른 토양 환경 간에 미생물 군집의 유전형적 유사성과 상대적 다양성을 비교하였다. 그 결과 소나무삼림토양이 다른 토양에 비해 상대적 다양성이 높은 것으로 밝혀졌으며, 경작지, 나지, 초지, 신갈삼림 순으로 다양성 정도를 나타내었다. 또한 유전형적 유사성의 정도에 따른 집괴 분석 결과 소나무삼림과 경작지 토양, 신갈나무삼림과 초지 토양 그리고 나지 등 세 부류로 구분되었다.

Keywords

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