• Journal of Bio-Environment Control
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• v.19 no.3
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• pp.147-152
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• 2010

Poor fruit set during winter period is one of the biggest problem in plastic tunnel watermelon cultivation. Hand pollination is inevitable to maximize fruit set of the winter watermelon. Productivity and viability of pollen grain and organic solvents for pollen storage were investigated. All cultivars produced more than 10 mg/flower except for 'Kumchun' cultivar. Pollen amount per flower were 13.8 mg in 'Bok' and 12.1 mg in 'Speedkul'. Germination rate of pollen grains incubated at $30^{\circ}C$ right after soaking in pentane solvent were 76% in 'Kumchun' as the lowest and 92% in 'Apollokul' as the highest. The pollen of 'Bok' showed the highest germination rate by 75% after a 15-day storage in pentane. All cultivars showed their pollen germination rate below 25% after a 24-day storage. Among the cuitivars, speed of pollen tube growth in vitro were relatively lower in 'Kumchun' and 'Sambokkul' by below $50\;{\mu}m/hr$. Pollen tube of these cultivars tended to burst during its elongation on the medium. Pollen stored 24 hrs in organic solvents showed 45, 39, 34, 23, and 19% of germination in pentane, ethyl ether, n-hexane, ethyl acetate, and acetone, respectively. Compared with light condition, pollen viability was higher in darkness during pollen storage in organic solvents. Pollen grain was susceptible to the organic solvent. The viability of pollen grains seems to be influenced greatly by duration of soaking pollen in organic solvent and the polarity of solvents. Organic solvent damages surface of pollen grain and extent of damage was varied by the solvents.

• Korean Journal of Medicinal Crop Science
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• v.4 no.3
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• pp.231-235
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• 1996

The experiment was conducted to determine pollen morphology of Chinese peony and its germination. The results were obtained as following ; Pollen shpae was usually large ellipse and the pol­len germination rate of Eui-seong Peony line was 71% which was lower than that of other Peony lines. In a bud, the pollen began to be observed on $7{\sim}9days$ before flowering and as getting on for flowering time, normal pollen and germination rates were higher. The pollen germination and elongation beganat 30 minutes after incubation on artificial medium and were completed after $2{\sim}3\;hours$. As the storage time went on, the pollen life span was shortened. The germination rate was 51% at 8 days-storage of room temperature. The germination rate was 48% ad 43% at 95 day-storage in $0^{\circ}C$ and $-15^{\circ}C$, respectively.

• Horticultural Science & Technology
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• v.17 no.3
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• pp.337-340
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• 1999

In order to evaluate stored apple pollen viability, in vitro germination test was performed on a microscope slide coated with the culture medium containing fluorescein diacetate (FDA). However, the inclusion of FDA to the culture medium declined pollen germination. Alternatively, the fluorochromatic reaction procedure was tested. The procedure involved dusting pollen grains onto drops of 10% sucrose solution containing 0.002% FDA and allowing them to accumulate fluorescein. Within 30 min after the fluorochromatic reaction, viable pollen grains clearly fluoresced under ultraviolet light. Both the in vitro germination test and the fluorochromatic reaction procedure revealed that stored apple pollen viability was not considerably decreased over storage up to at least 39 months. Of the cultivars examined by both methods, 'Fuji' and 'Senshu' pollen viability was highest, 'Tsugaru' was intermediate, and 'Jonagold' was lowest. The fluorescing percentages appeared approximately comparable to the germination percentages except for the 'Senshu' pollens stored for 3 months, although the fluorescing percentages was slightly higher than the germination percentages. Strong and highly significant correlations were found between the two methods. It can thus be concluded that the fluorochromatic reaction procedure provides a convenient and reliable evaluation of stored apple pollen viability.

• Food Science and Preservation
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• v.16 no.4
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• pp.525-533
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• 2009

To explore cattail pollen powder as a functional food ingredient, we analyzed the general components of pollen powder, tested changes in the physical properties of dough containing the powder, and investigated the process ability of powder-containing dough in bread making by adding 3%, 6%, or 9% by weight of pollen powder to wheat flour. Cattail pollen powder consisted of (all w/w) 12.7-13.2% water, 15.7-17.8% crude protein, 1.3% crude fat, 7.5-7.7% free sugar, 14.7-18.6% crude fiber, 3.4-4.9% pollen, and 49.7-55.9% soluble nitrogen-free extract (NFE). Analysis of the physical properties of dough mixed with pollen powder showed that as more pollen powder was added, the absorption rate increased, but dough stability decreased. With increasing levels of cattail pollen powder, the falling number decreased, and amylase activity increased. Fermentability was highest in dough made with 3% by weight of pollen powder, and the bread product made from such dough had the greatest volume. As more cattail pollen powder was added, the moisture activity in dough tended to decrease to a greater extent than seen in control dough, and this tendency increased with time. We found that longer storage periods were associated with greater hardness and springiness, which indicated degradation in product quality. Therefore, it is suggested that bread products containing cattail pollen powder should be consumed within 3 days of preparation. In a taste survey, bread baked with 3% (w/w) cattail pollen powder scored highest in all questionnaire items.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 1994.07a
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• pp.23-24
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• 1994

본 연구는 성숙과의 저장중에 세포벽분해효소가 세포벽을 분해해서 물성의 변화를 유발함으로 일어나는 과실의 연과가 품질과 저장성의 저하 뿐만 아니라 영양적, 경제적 손실을 초래한다는 점을 고려하여 대추의 성숙중에 일어나는 연화현상을 연구하고자 성숙에 따른 경도, 세포벽분해효소의 활성, 세포벽 다당류 pectin질, 비섬유성 중성당 및 조직의 변화를 조사하였다. 경도는 대추의 숙성에 따라 감소하였고, polygalacturonase와 $\beta$-galactosidase의 활성은 각각 변색기와 완숙기에 나타난 이후 급격히 증가하였다. 세포벽 다당류인 pectin질과 알칼리 지용성 hemicellulose는 완숙기가지 증가햐였으나 cellulose는 완숙기에 산가용성 hemicellulose와 cellulose를 제외한 세포벽 다당류의 함량은 다소 감소하였다. 대추의 세포벽 비섬유성 중성당으로 rhamnose, arabinose, xylose, mannose, galactose, glucose가 동정되었고, 성숙동안에 pectin질에서는 arabinose, mannose, galactose와 총 비섬유성 중성당의 함량이 감소하였고, 산가용성 hemicellulose에서는 xylose와 mannose가 뚜렸하게 증가하였으나 중성당은 변화없었으며, 알칼리 가용성 hemicellulose에서는 성숙에 따른 변화가 거의 없었다. pectin질의 경우 수용성 pectin, EDTA 용해성 pectin 및 총 pectin은 성숙중에 증가하는 경향이있으나 불용성 pectin은 감소하는 경향이였으며 과숙기에는 불용성 psctin EDTA용해성 pectin 및 총 pectin의 함량은 모두 현저히 감소하였다. 대추의 성숙중 조직에서는 pectin질로 구성된 중충의 붕괴현상이 뚜렸하게 나타났다.발이 절실히 필요한 실정이다. 이러한 배경으로 본 강연에서는 효소적갈변 저해제의 개발과 그들의 식품가공에의 적용 현환 및 화장품, 의약품으로의 응용에 대해 설명하고자 한다.L주에 비해 S주는 수정후 용과가 더 심하다. 9) 화분관의 행동은 수정력과 완전히 일치된다. 즉 L-selfing, $L{\times}L$, S-selfing, $S{\times}S$등의 부적법 수분에서는 화분관은 화주의 미중에서 정지되지만 $L{\times}S$, $S{\times}L$,에서는 수분 약 40-50분 후이면 화분관은 자방까지 도달된다. 10) S주는 웅본으로 오인되어 있지만 인위적법수분을 하면 수정력이나 화분관의 행동은 L주에서와 동일하다. 11) S화분은 완전하지만 L화분은 약 70%가 내용공허한 Adortive pollen 이다. 12) L화분중 나머지 30%도 S화분에 비해서 염색도가 낮은것이 많고 S화분 같이 농염되는 것은 극히 소수이다. 13) 본장물은 분화가 고도로 진행된 전형적인 이형예작물이여 마치 Dimorphism 에서 Dioecious 에로 이행되는 수가 있다는 것을 표시하는 증거가 되는 것 같다. 다소 높은 산소농도 3%~5% 이산화탄소 농도 5~8%에서 저장하는 것이 효과적일 것으로 판단되었다.철쭉군목으로 대표되나 군단이 하의 군목들은 다소 차이를 보이는 것으로 나타났다. 중간상인이론의 수정이 필요하다고 본다.가\ulcorner 본 논문에서는 표면적 형태에도 불구하고 [-wh]의미의 겹의문사는 병렬적 관계의 합성어가 아니라 내부구조를 지니지 않은 단순한 단어(minimal $X^{0}$ elements)로 가정한다. 즉, [+wh] 의미의 겹의문사는 동일한 구성요 소를 지닌 병렬적 합성어([

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.6
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• pp.443-448
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• 2001

This study was carried out to obtain the basic informations on the characteristics of gametophytes formation and microspore germination in Astragalus membranaceus Bunge. Pollen mother cells passed through meiosis when the flower bud length reaches around 3.5 mm, thus creating the tetrad when it is 4.0 mm long. Pollen attains full growth when the bud is about 10.0 mm long and the anther is found to dehisce when the length of tate bud reach around 12.0 mm. Embryo sac develops at a similar speed as pollen did and it attains its full growth when the bud is about 10-12 mm long. After being stored at 4$^{\circ}C$ or -4$^{\circ}C$, the pollen maintained its germination ability to almost full extent by the 30th day after store. However, the germination rate at room temperature (23~28$^{\circ}C$) decreased below 3％ by the 3rd day of storage and so did the germination speed.

• Applied Biological Chemistry
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• v.29 no.4
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• pp.416-421
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• 1986

방사선을 이용한 화분의 저장방법을 개선하기 위하여 저장중인 메밀 화분으로 부터 곰팡이를 분리, 동정하고 이들의 방사선 감수성을 조사하였다. 분리, 동정된 곰팡이는 Penicillium jenseni, Aspergillus oryzae, Asp. niger, Alternaria SP. 으로 이들 포자의 $D_{10}$ 값은 각각 0.43, 0.54, 0.58, 0.97kGy 이었고, 유도선량은 각각 0.23, 0.18, 0.23, 0kGy이었다. Pen. jenseni 포자의 방사선 감수성은 용존산소하에서 증가하였으며 이에 열처리를 병용하였을 때는 유도선량이 현저하게 감소하였다. 화분 중의 총세균과 곰팡이의 $D_{10}$ 값은 각각 1.25kGy와 $1.50{\sim}4.30kGy$이었다.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.496-501
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• 2009

In order to promote the efficiency of strawberry breeding programs, pollen viability of strawberry, 'Seolhyang' was investigated using the fluorochromatic reaction test and seed set under controlled environment. Pistil receptivity was also assessed by hand pollination. Four varieties including 'Maehyang' were used for the test of pistil receptivity with 'Seolhyang' as a pollen parent. Pollen viability remained high for several days under dry conditions as below 33% relative humidity while the greatest loss of viability occurred at 76% relative humidity. The viability was rapidly decreased at high humidity and almost all grains were unviable in 7 days after storage. Pollen viability does not appear to be drastically reduced if the relative humidity is low. Therefore, humidity is more important factor than temperature for the pollen viability in Fragaria${\times}$ananassa. The rate of seed set by hand pollination lasted higher than the average of 77.2% from 2 to 8 days after emasculation when the daily average temperature was around $15^{\circ}C$ in plastic house. It began to decline gradually from 10 days and had decreased dramatically after 12 days except several cultivars. Based on the daily mean accumulated temperature, it is recommended to have an artificial pollination between the range of $45{\sim}140^{\circ}C$ after the emasculation to increase the rate of seed set in strawberry.

• Food Science and Preservation
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• v.14 no.1
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• pp.87-93
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• 2007

This study investigated the antimicrobial effect of germinated acorn pollen solution on harmful food-borne microorganisms. The antimicrobial activity when 8% (w/v) acorn pollen in 10% (w/v) sugar solution was extracted at $30^{\circ}C$ for 4 days. The minimal inhibitory concentration of this germinated acorn pollen solution was $40\;{\mu}L/mL$ for Gram-positive bacteria and $30\;{\mu}L/mL$ for Gram-negative bacteria. Acetic and lactic acids were present at high levels in germinated acom pollen solution. As pollen germination releases heat, the antimicrobial activities are heat-stable. The activities are tolerant of low pH. In summary, acorn pollen germination solution showed active antibiosis and should be developed as a natural preservative material.

• KSBB Journal
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• v.19 no.3
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• pp.174-177
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• 2004

A method of collection and long-term storage of viable lily (Lilium longiflorum) pollen grains were developed for their in vitro growth and transformation in consistency. Petroleum ether, n-heptane, cyclohexane and benzene, as pollen collection medium, were determined less toxic to pollen growth in vitro than others tested. Pollen grains, however, lost their growth activity if stored in these solvents more than a week, So, a serial performance, that is, pollen grain collection in these solvents, air-drying and immediate transfer to low temperature condition was determined desirable for keeping the viability much longer. Pollen grains from this storage showed a successful transformation in vitro with a cDNA encoding tissue plasminogen activator (TPA) protein using Agrobacterium via vacuum infiltration according to western blotting analysis.