• Title/Summary/Keyword: 형광분석

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A Characteristics Modelling of Start-up and Frequency of Fluorescent Lamp by Empirical Values (실험치에 의한 형광램프의 기동 및 주파수특성 모델링)

  • 함중걸;백수현;김일남;맹인재
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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    • 1996.11a
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    • pp.21-25
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    • 1996
  • 형광램프 시스템의 설계에서 안정기의 실패는 형광램프의 특성을 고려하는 것이 매우 중요하다. 특히, 시동시 과도특성에 근거한 형광램프의 동작모델을 등가회로로 알아내기 위해 실험에 의해 얻어진 결과를 등가희로 모델링과 Pspice 시뮬레이션에 의해 나타난 결과와 비교 분석하였으며, 특히 고주파용 형광램프의 동작 특성은 동작주파수, 형상, 램프 전압 및 전류값에 따라 차이가 많기 때문에 형광램프의 특성은 안정기의 특성을 고려한 형광램프 시스템에 동작특성 정합성에 매우 중요하다. 형광램프에 대한 고주파 특성으로 가장 많이 사용되는 주파수 영역(12Khz - 50Khz)에 대해서 실험, 분석한 결과 형광램프의 부(負)임피던스 동작특성을 파악하고 고주파 형광램프의 최적 특성치를 선택할 수 있는 방법을 제시하였다.

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Development of An Integration Management System of Analyzing Fluorescence Images on Smart Phone (모바일용 형광이미지 분석 통합관리 시스템 개발)

  • Cho, Mi-Gyung;Shim, Jae-Sool
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2012.05a
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    • pp.916-919
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    • 2012
  • Bioimaging that can be imaging phenomena within cells of a molecular size have been advanced in technology. We can observe clearly DNA and proteins using a confocal microscope. Currently biological fluorescent imaging area is used essentially for diagnosis and treatment in health and clinical care field. In this paper, we developed an integration management system of analyzing fluorescence images on smart phone. It can support a user to analyse fluorescence images anytime anywhere. And our system is based on client-server configuration and has functions that can figure intensity of fluorescence images and manage many imaging data. Proposed system can be a mean of ubiquitous health because it helps a doctor diagnose by analyzing fluorescence images of emergency patients without time and space restrictions.

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Trace Analysis of Uranium in Aqueous Samples by Laser-induced Fluorescence Spectroscopy (레이저를 이용한 용액중의 미량 우라늄 분석)

  • Jung, Kwang-Woo;Kim, Jeong-Moog;Kim, Cheol-Jung;Lee, Jong-Min
    • Nuclear Engineering and Technology
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    • v.19 no.4
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    • pp.242-248
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    • 1987
  • A sensitive, direct method for the determination of trace amounts of uranium in solution has been developed utilizing laser-induced fluorescence spectroscopy and a fluorescence enhancing reagent 'Fluran.' Standard addition technique is incorporated into the analysis to eliminate sample matrix effects. Analytical data show that a detection limit of 0.1 ppb (part per billion) uranium has been achieved and the precision of the analysis is in the range of 5% relative standard deviation. Results using the laser fluorescence method on many sets of unknown samples have been compared against corresponding values determined by other methods.

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Characterizing Fluorescence Properties of Dissolved Organic Matter for Water Quality Management of Rivers and Lakes (하천 및 호소 수질관리를 위한 용존 자연유기물질 형광특성 분석)

  • Hur, Jin;Shin, Jae-Ki;Park, Sung-Won
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.9
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    • pp.940-948
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    • 2006
  • Fluorescence measurements of dissolved organic matter(DOM) have the superior advantages over other analysis tools for applying to water quality management. They are simple and fast and require minimal pretreatment of samples. Fluorescence index($F_{450}/F_{500}$), synchronous spectra, and fluorescence excitation-emission matrices(EEM) of various DOM samples were investigated to discriminate autochthonous/allochthonous composition, protein-like fluorescence, fulvic-like fluorescence, humic-like fluorescence, terestrial humic-like fluorescence by comparing among the real DOM samples of different origins with the help of literature. The samples used included standard purified DOM, lake, river and wastewater treatment effluent. The relative distribution of various DOM composition was derived from the ratios of each fluorescence region. The results were very consistent with those expected from the sample properties. Allochthonous and terrestrial humic-like fluorescence were more prominent in the samples with abundant soil-derived DOM components. In addition, the protein-like fluorescence property was more pronounced in the samples where strong algal or microbial activities were expected. It was also shown that the ratio of protein-like/terrestrial humic-like fluorescence obtained from synchronous spectrum and fluorescence EEM could be used as an indicator for the evaluation of wastewater treatment on the downstream water quality of rivers and for the prediction of the degree of algal/microbial activities in lakes. It is expected that the results of this study will provide the basic information to develop the future water quality management techniques using DOM fluorescence measurements.

A study on lifetime characteristics for dimming of 32W fluorescent lamp (32W 형광등의 디밍에 의한 수명특성 연구)

  • Jin, Sang-Min;Kim, Hyun-Joong;Ku, Sung-Mo;Yi, Chin-Woo
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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    • 2009.10a
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    • pp.185-187
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    • 2009
  • 본 논문에서는 디밍으로 인한 32W 형광등의 수명특성과 동정을 실험을 통해서 분석하였다. 기존의 형광 램프용 전자식 안정기 내부의 인버터 공진특성을 이용해 주파수를 변환시켜 소비전력을 낮추면서 형광등의 조도를 조정하였다. 조정된 형광등의 안정기를 소비전력의 퍼센트별로 분류해서 조도특성을 위주로 수명의 측정을 실시하였다. 수명특성 실험은 2개월간 실험실 내에 형광등을 설치 할 수 있는 실험대를 제작하였으며 주기적으로 형광등의 주위온도와 조도의 측정을 실시하였다. 본 논문에서 분석한 형광등의 수명특성 결과는 향후 형광등 전용 디밍 전자식 안정기 설계 시 활용 될 수 있을 것이다.

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Development of a real-time Analysis System of Microchip Fluorescence Images based on Server-Client (서버 클라이언트 기반의 실시간 마이크로칩 형광 이미지 분석 시스템 개발)

  • Cho, Migyung;Shim, Jaesool
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.17 no.5
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    • pp.1239-1244
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    • 2013
  • In the field of clinical medicine and research, the analysis of such as protein and DNA at the molecular level and even at the cell level are necessary for disease diagnosis and treatment. In many cases, a real time image of samples is needed for the accurate analysis and manipulation of samples since experimental samples are degenerated with time. In this research, a three-dimensional fluorescence microscope device was developed for taking images of protein and DNA inside a single cell and the server-client based image analysis system was made for an integrated management of the real-time images taken from the microscope device. The system consists of a fluorescent measurement device, the associated software and a client program on smartphone. The developed system allows doctors or experimental managers to receive and look at the real-time experimental images taken from the samples of patients anywhere in the emergency, to analyze results and to instantly diagnose the disease and to transfer the results to the patients. As a result, the system is able to be utilized in the implementation of ubiquitous health as well.

Evaluation of Cell Viability and Delivery Efficiency in Electroporation System According to the Concentrations of Propidium Iodide and Yo-Pro-1 (전기천공시스템에서 Propidium Iodide와 Yo-Pro-1의 농도에 따른 세포 생존율과 전달효율 평가)

  • Bae, Seo Jun;Im, Do Jin
    • Korean Chemical Engineering Research
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    • v.57 no.6
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    • pp.898-906
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    • 2019
  • In this study, basic research was conducted to provide guidelines for selecting fluorescent dye and using proper concentration of fluorescent dye to use evaluation of cell viability and fluorescent dye delivery efficiency. Propidium iodide and Yo-Pro-1 were used as fluorescent dyes. In the evaluation of cell viability and the efficiency of delivery using Propidium Iodide and Yo-Pro-1, the histogram of each fluorescent dye was different depending on the type of fluorescent dye and the concentration used. These results were related to the characteristics of the fluorescent dyes used. This was related to the properties of the fluorescent dyes used. From these results, it was found that the analytical results depending on the characteristics of the fluorescent dyes used in the cell analysis. The effect of the fluorescent dye on the cell was confirmed, but it was confirmed that it did not affect the analysis result. In addition, the influence of interference between fluorescent signals was confirmed when two or more kinds of fluorescent dyes were used for analysis. The higher the concentration of Yo-Pro-1 was, the larger the effect of interference was, and the concentration of Propidium Iodide did not affect the interference of fluorescence signal. This study confirmed that the evaluation of the cell viability and the evaluation of the delivery efficiency were influenced by the type and concentration of the fluorescent dyes and it was related to the characteristics of the fluorescent dyes. Based on the results, appropriate concentrations of fluorescent dyes suitable for evaluation of cell viability and delivery efficiency were suggested.

Development of Analytical Method of Organophosphates in Sea water by Finger Printing Fluorescence Spectroscopy (형광분석을 이용한 지문방식의 해수중 유기인의 분석 방법)

  • PARK Mi-Ok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.3
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    • pp.377-387
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    • 1997
  • Primary fluorescence characteristics of ten standard solutions of organophosphates, sea water, and water from agricultural land were investigated by fluorescence contour. All the standard solutions of organophosphates has shown characteristics countours. Their emission maxima were shown between 296 nm and 437 nm. According to their numbers of emission maxima on the fluorescence contours, the organophosphates can be categorized in two different groups. Ateric and Diazinon are the first group with two emission maxima. DDVP with other seven standard organophosphates belong to the second group. The second group has two subgroups. One is characterized by the similar emission and excitation maxima, which are 310 nm and 280 nm, respectively. Those are DDVP, Hinosan, Kitazin, Locsion, Meta. The other sub-group shows quite different emission and excitation maxima from the first sub-group. They are Monopho, Thaconyl and Gropho and their emisson maxima were in far longer (437 nm) or shorter wavelength (296 nm). From agricutural samples, one of the investigated organophosphates was detetected by its characteristic retention time $(t_r=12min)$. HPIC-fluorescence data gave an additional parameter for differentiation between two organophosphates which has similar excitation and emission maxima.

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Observation of the Second Phases in a Red Phosphor $SrTiO_3$:Pr,Al by using Transmission Electron Microscopy ($SrTiO_3$:Pr,Al 적색 형광체에서 TEM을 이용한 2차상의 관찰)

  • 이정용;이호성;정연철;전덕영
    • Journal of the Korean Ceramic Society
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    • v.38 no.3
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    • pp.225-230
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    • 2001
  • 저전압용 형광체로 주목받고 있는 SrTiO$_3$:Pr,Al 형광체를 고상반응법으로 제조하였다. 부활성체 Al이 23 mol% 첨가되었을 때 SrTiO$_3$:Pr 형광체는 최대 발광 강도를 보여주었다. 최대 발광 강도를 보인 형광체에 대해 제한시야회절상과 투과전자현미경을 이용하여 SrTiO$_3$:Pr,Al 적색 형광체내 2차상 형성에 대한 연구를 행하였고 또한 에너지 분산 분광 분석을 행하여 SrTiO$_3$:Pr,Al 형광체내 주입된 부활성제 Al의 함량을 결정하였다. 제한시야회절상의 결과에 의하면 SrTiO$_3$:Pr,Al 적색 형광체내에 단사정 SrAl$_2$O$_4$, 삼사정 Ti$_4$O$_{7}$, 육방정 SrAl$_{12}$O$_{19}$가 2차상으로 존재함을 보여 주었다. 에너지 분산 분광 분석 결과 부활성제 Al의 함량 중 일부는 SrTiO$_3$:Pr,Al 적색 형광체 격자에 고용되고 일부 Al은 SrAl$_{12}$O$_{19}$와 SrAl$_2$O$_4$의 2차상을 형성하는데 소모됨을 보여주었다.주었다.

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A Study on the Development of Quantitative Analysis Methods to Characterize the Transport of Microplastics in Saturated Porous Media (포화 다공성 매체에서 미세플라스틱 이동 특성 규명을 위한 정량분석 방법 개발 연구)

  • Suhyeon Park;Minjune Yang
    • Proceedings of the Korea Water Resources Association Conference
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    • 2023.05a
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    • pp.376-376
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    • 2023
  • 전 세계적으로 중요한 담수 자원인 지하수의 미세플라스틱 오염에 대한 우려가 커지고 있다. 지하수 환경에서 미세플라스틱의 오염을 예측하고 평가하기 위해 대수층 내 현장 실태조사가 수행 중에 있으며, 실험실 규모의 컬럼 실험을 통해 지하수에서 미세플라스틱 이동 메커니즘을 조사하는 연구들이 수행되고 있다. 이러한 연구들은 많은 개수의 분석 시료를 동반하며, 환경 중 미세플라스틱 정량분석을 위해서 고가의 분석기기(라만분광기, 푸리에 변환 적외선(FTIR) 분광기, 열분해 가스크로마토그래피 질량분석기)를 사용하여 플라스틱의 종류를 판별하고 개수를 측정하고 있다. 또한, 컬럼 실험을 수행한 대부분의 선행 연구에서는 미세플라스틱 정량분석을 위해 탁도 분석, 분광광도계를 이용한 흡광도 분석, 현미경을 이용한 계수 방법 등을 이용하여 고가의 분석기기를 사용하지 않고 연구를 수행하였다. 하지만, 이러한 방법들은 유체 속 다른 물질이 포함되어있을 경우에 민감하고 농도를 비율 혹은 개수로 표현하기 때문에 질량 측면에서 미세플라스틱의 농도를 과소·과대 평가할 수 있다. 특히, 현미경을 이용한 계수 방법의 경우에는 분석에 많은 시간이 소요된다는 단점이 있다. 위에 언급한 다양한 분석법들의 단점들을 보완하기 위하여, 본 연구에서는 대수층 내 미세플라스틱 이동 특성을 규명하기 위한 실내 실험에 사용될 수 있는 형광이미지 기반의 미세플라스틱 정량분석법을 개발하였다. Nile Red 형광염료를 이용하여 미세플라스틱을 염색하고 사진을 촬영하여 미세플라스틱 시료의 질량과 미세플라스틱 형광이미지의 형광강도 간 상관관계를 분석하였다. 또한, Nile Red로 염색된 미세플라스틱 입자의 수중 노출 테스트를 진행하여, 실내 대수층 모의실험 시 미세플라스틱 질량을 정량화할 수 있는 적용 가능성을 평가하였다. 상관 분석 결과, 미세플라스틱 질량과 이미지의 형광강도는 높은 상관관계를 보였으며, 수중 노출 실험 전과 후의 미세플라스틱 입자의 형광강도 차이는 미미한 것으로 나타났다. 이러한 연구결과를 통해 본 연구에서 개발된 미세플라스틱 정량분석 방법이 포화 다공성 매체로 구성된 컬럼실험 시 유출수의 미세플라스틱 질량 추정에 유용하게 사용될 것으로 생각되며, 대수층 내 미세플라스틱의 이동 특성 규명 연구에 많은 도움이 될 것으로 기대된다.

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