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Biochemical Study on Soft-rotted Sweetpotatoes (연부(軟腐)고구마의 생화학적(生化學的) 연구(硏究))

  • Chun, Jae-Kun
    • Applied Biological Chemistry
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    • v.10
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    • pp.47-61
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    • 1968
  • Sweetpotato infected by Soft-rot, Rhizopus nigricans, has been investigated for its biochemical changes. The results of the present experiment are summarized as follows: 1. From the Soft-rotted tuber, nineteen Ehrlich's positive substances and three fluorescent compounds which had not been found in a healthy tuber were detected with thin layer chromatography. 2, Ipomeamarone and umbelliferone, each of which are predominating substances among the Ehrlich's positive and fluorescent compounds, were isolated by silica gel column chromatography and studied for their identities. 3. Time course biosynthesis of the metabolites was studied and discussed for their possible biosynthetic pathways. 4. Among the metabolites, ipomeamarone and ipomeamarone-like substances have enzyme: inhibitive action against ${\alpha}$-amylase and probably other enzymes; the inhibition type was determined as the uncompetitive one. 5. Ipomeamarone obtained from soft-rotted sweetpotato was proved to have an uncoupling action as 2, 4-dinitrophenol.

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Basic Study Of Composting on Agricultural Animal Waste (농축산폐기물(農畜産廢棄物)의 퇴비화(堆肥化)에 관한 기초적(基礎的) 연구(硏究))

  • Jung, Bing Soo;Gang, Young Tae
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.5 no.2
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    • pp.27-34
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    • 1985
  • Pig manure mixed with straw, sawdust, packing paper and chaff was composted in a batch type enclosed composter without regular mixing for 1 week. The maximum decomposition was obtained in the temperature of $70^{\circ}C$ with an initial moisture content of 50 to 65 %, initial pH of 7 to 8, 0.7 to $2.0l/min{\cdot}kg$ per volatile matter of air supplied and C/N ratio of 60 to 70. The maximum carbon dioxide content in the produced gas was 65 to 85 mg/hr-vm at 45 to $53^{\circ}C$.

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Tests on the Flexural and Shear Behavior of Partially Prestressed Concrete Beams(I) -About the Ultimate State Behavior and Prestressing Indicies (부분(部分) 프리스트레스트 콘크리트 부재(部材)의 휨 및 전단(剪斷) 실험(實驗)(I) -극한상태(極限狀態)의 거동(擧動)과 프리스트레싱 지표(指標)에 대(對)하여)

  • Chang, Sung Pil;Kang, Won Ho
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.9 no.4
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    • pp.27-39
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    • 1989
  • The partially prestressed concrete members have intermediate degree of prestressing between the reinforced and fully prestressed concrete members. Partially prestressed concrete members take advantages of both the merits of fully prestressed concrete members under the service load state and those of reinforced concrete members under the ultimate load state. Total 12 partially prestressed concrete T-beams were m4de and tested to find the effects of prestressing ratio, stirrup arrangement and shape of cross section on the flexural and shear behavior of the partially prestressed concrete beams. In this paper, the test procedure and the ultimate behavior of test beams are described. The validity of prestressing indices is checked by analyzing test results.

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Fatigue Crack Growth of Welded Joints for the Rail of Railroad (철도궤조(鐵道軌條)의 용접연결부(鎔接連結部)에 대한 피로균열성장(疲勞龜裂成長))

  • Chang, Dong Il;Bak, Yong Gul;Kyung, Kab Soo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.6 no.3
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    • pp.75-86
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    • 1986
  • The welded joint of the rail of railroad had shown the complicated behaviour of fatigue crack growth due to the effect of low toughness, residual stress, welding stress and strain etc. resulting from welding. Also, the welding connection has been necessary as making longer the rail, thus fatique fracture has often occurred at welded joints. From above, in this paper, faigue test was done at base metal, gas pressure and thermit welded joints to give the basic data to construct S-N diagrams. From this, it was known that the base metal was better than welded joint and gas pressure better than thermit welded joints in the resistance against the behaviour of fatigue crack growth. And it was also found that it is very dangerous to control fracture only by the method of material mechanics.

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Development of several methods to remove Cadmium from soil contaminated with Cadmium (Cadmium 오염토양(汚染土壤)에서 Cadmium 제거방법(除去方法)의 개발(開發))

  • Choi, Jyung;Lee, Jyung-Jae;Hur, Nam-Ho
    • Korean Journal of Environmental Agriculture
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    • v.10 no.2
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    • pp.128-132
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    • 1991
  • This study was carried out to find a method to remove cadmium from soil and/or attenuate in Cd saturated Soil The chemical form of Cd was influenced by the physico-chemical properties of soil and the adsorption of Cd by soil conformed to the Langmuir isotherm. The order of Cd contents in chemical fractions extracted by several reagents was EDTA > NaOH > $HNO_3$. Flooding, liming and the addition of chelating agents and clay minerals to the contaminated soil seems to desirable in alleviating the harmful effects of Cd on plant growth by reducing Cd content in the $KNO_3$ fraction.

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Studies on the Utilization of Fish Flour -Part 1. Removal of TMA and Lipids from raw Cololabis Saira- (어육단백질(魚肉蛋白質)(Fish Flour)의 실용화(實用化)에 관(關)한 연구(硏究) -(제(第)1보(報)) TMA 및 지방(脂肪)의 제거(除去)-)

  • Ryu, In-Deok;Kim, Dong-Soon;Yang, Ryung;Ryu, Ju-Hyun
    • Korean Journal of Food Science and Technology
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    • v.8 no.4
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    • pp.212-218
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    • 1976
  • For removal of trimethylamine (TMA) which is generally accepted as the main component of marine fish oder and the lipids in marine fish which contribute directly or indirectly to the odor of fish flour, extractions of raw Cololabis saira were carried out with Ethanol-Water Mixture or Absolute Ethanol. Results obtained are as follows: 1. Efficient extraction of TMA from raw Cololabis saira took place when final concentration of ethanol was $40%{\sim}55%$. 2. When successive extraction of raw Cololabis saira was carried out with boiling absolute ethanol, the extraction proceeded in two distinct stages: a) In the first stage, efficient dehydration of raw Cololabis saira and efficient TMA extraction took place simultaneously. b) In the second stage, efficient lipid extraction followed after the dehydration stage.

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Preparation of Stand Volume Table by the Multivariate Statistical Analysis Method (다변량해석법(多變量解析法)을 이용(利用)한 임분재적표조제(林分材積表調製))

  • Kim, Dong-Chun
    • Journal of Korean Society of Forest Science
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    • v.19 no.1
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    • pp.49-54
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    • 1973
  • Surveys of stock volume on steep and vast expanse of mountains, involves various difficulties. And it is extremely uneconomical in forest management point of view, to spend lots of time and man power for surveying such tree volume as the value is much cheaper in comparison with volume and weight. Therefore, a stand volume table estimate easely stock volume per hectare basis from aerial photographs was prepared and correlations to stand volume among factors affecting tree volumation, were studied. Data were 114 places selected from planted Korean white pine, Pinus koreiensis Sieb. et Zucc. stands in Kwangnung Experiment Forest and were computed and analysed by the means of the quantification in the multi-variate statistical analysis. Electronic Data Processing System was applied for data processing at Korean Instiute of Science and Technology. Coefficients of multiple correlations of stand volume table was ranged 0.85~0.88.

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Immobilization of Thermolysin and Application of the Immobilized Thermolysin to Cheese-making (Thermolysin의 고정화(固定化)와 고정화(固定化) Thermolysin의 Cheese제조(製造)에의 이용(利用))

  • Yun, Se-Eok
    • Korean Journal of Food Science and Technology
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    • v.20 no.2
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    • pp.245-251
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    • 1988
  • Thermolysin was immobilized on Dowex MWA-1 with 10% glutaraldehyde and incorpo rated into a fluidized-bed continuous coagulation scheme to make Cheddar type cheese. The activity yield of thermolysin was 25%. The immobillized thermolysin was stable at $60^{\circ}C$ in the presence of 1/200M calcium ions and the half-life value is 16 days at the temperature. Raw milk alkalified to pH 7.0 was passed through a column of thermolysin beads at $55^{\circ}C$, cultivated with Streptococcus cremoris and allowed to coagulate. A typical milk curd was formed to make Cheddar type cheese, avoiding troublesome microbial contamination successfully during continuous hydrolysis process. During ripening of this cheese for 6 months at $10^{\circ}C$, its ripening ratio and taste were similar to those of cheese prepared by the traditional method.

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Studies on the Production of Polysacrhacides by Agrobacterium radiobacter (Agrobacterium radiobacter에 의(依)한 다당류(多糖類) 생산(生産)에 관(關)한 연구(硏究))

  • Lee, Ke-Ho;Shin, Hyun-Sung
    • Applied Biological Chemistry
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    • v.26 no.2
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    • pp.110-118
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    • 1983
  • A slimy non-spore-forming bacterium strain SAF-C isolated from bean stem and root was motile with flagella and identified to one of Agrobacterium radiobacter. Studies were made on the conditions necessary for maximal production of this acidic succinoglucan polysaccharide by this strain in shaken culture. Much production was observed with yeast extract,$(NH_4)_2\;HPO_4$, distillers' dried solubles(D.D.S.), as nitrogen source in the medium composed of 4% glucose, 0.5% nitrogen source, 0.3% $CaCO_3$. The yield was greatest with yeast extract and decreased in order with the above nitrogen source from 22.9% to 9.6 percent. A polysaccharide was produced in a yield of about 25% in a medium composed of 3% glucose, 0.4% D.D.S., 0.5% $K_2\;HPO_4$, 0.01% $MgSO_4{\cdot}7H_2O$.

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The Adsorption of N-methylcarbamate Insecticides on Soils (N-methlycarbamate 계(系) 살충제의 토양중(土壤中) 흡착(吸着))

  • Kim, Jang-Eok;Hong, Jong-Uck
    • Applied Biological Chemistry
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    • v.28 no.3
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    • pp.124-130
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    • 1985
  • Adsorption experiments of N-methylcarbamate insecticides on soils were carried out as a function of soil pH ana soil organic matter content with wet-and dry-land soils that were either oxidized or non-oxidized. The results obtained may be summarized as follows: The adsorption of N-methylcarbamate insecticides on soils was nearly leached to equilibrium after shaking for 12 hours. The adsorption of N-methylcarbamate insecticides was higher on sandy clay than sandy loam. The presence of organic matter in soil increased the adsorption of N-methrlcarbamate insecticides on soils. The mode of isothermal adsorption of N-methylcarbamate insecticides on soils was coincident with the Freundlich equation. Little effect of soil pH on the adsorption might be interpreted as that the adsorption was due to physical adsorption between N-methylcarbamate molecules and soil surface.

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