• Journal of the Korean Society of Food Science and Nutrition
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• v.12 no.3
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• pp.207-211
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• 1983

An attempt was made to find out the possibility of utilizing water-melon seed as resources of food fats and protein. The water-melon seed contained 40.40% of crude fat and 28.36% of crude protein. The lipid fraction obtained by silicic acid column chromatography was composed of about 97.35% neutral lipid, and the main components of neutral lipid by thin layer chromatography were triglyceride(50.40%), diglyceride(21.84%) and sterol(11.48%). The predominant fatty acids of total and major lipid classes were linoleic acid(55.30-67.85%), palmitic acid(12.07-28.12%) and oleic acid(9.06-16.40%), whereas stearic acid and linolenic acid were detected as small amounts. The salt soluble protein of watermelon seed was highly dispersible in 0.02M sodium phosphate buffer containing about 0.7M $MgSO_4$, and the extractability of seed protein was about 27%. Glutamic acid and arginine were major amino acids, and the essential amino acids such as lysine, threonine, valine, methionine, isoleucine, leucine and phenylalanine were also detected. The electrophoretic analysis showed 6 bands in water-melon seed protein, and the collection rate of the main protein fraction purified by sephadex G-100 and G-200 was 52.4%. The amino acids of the main fraction protein were also mainly composed of glutamic acid and arginine. The molecular weight for the main protein of the water-melon seed was estimated to be 120,000.

• Polymer(Korea)
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• v.38 no.3
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• pp.364-370
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• 2014

The retinal pigment epithelium (RPE) plays an important role in maintaining a healthy retina and the degeneration of RPE caused a number of retinal diseases. The transplantation of RPE has recently become a possible therapeutic modality for retinal degeneration. To transplant RPE cells securely, substrates are essential, and then as a substrate, we fabricated films using silk that has unique mechanical properties and biocompatibility. After the FTIR spectra, contact angle and biodegradation of silk films were confirmed, RPE cells were seeded and the influence of RPE cells on silk films was examined. We measured the cell adhesion, cell viability, morphology and specific mRNA expression by MTT assay, SEM, immunofluorescence and RT-PCR. In this study, we confirmed that attachment, proliferation and phenotype maintenance of RPE cells cultured on silk films were great, and thereby we were able to confirm the potential applications of silk films as tissue engineering carrier for regeneration of retina.

• Journal of the Korea Concrete Institute
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• v.24 no.2
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• pp.165-172
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• 2012

In this study, the physical, mechanical, structural, and environmental performances based on field measured data were evaluated to check the suitability of concrete for ecological preservation and cultivation of a hydrophilic environment. More specifically, the study is focused on developing an environmentally friendly functional concrete with river ecology restoration and natural river early formation capabilities. The mechanical performance evaluation results showed that the increase in mix rate of the PVA (Poly Vinyl Alcohol) reinforcement fibers and silica fume caused an increase in the strength. The optimal mix rate was found to be 0.05 volume % PVA fiber and approximately 10% silica fume. The frost resistance evaluation showed that superior performance was gained when 0.05 volume % PVA fiber and 15% silica fume was mixed simultaneously. In the structural performance evaluation, the bending strength was improved by 47.7% compared to plain concrete when mixed with 0.05 volume % PVA fiber. The flexural toughness also saw significant improvement. The environmental monitoring of field performance showed that grasses germinated most rapidly, but the growth of red poppies, a plant that germinates in the spring, was most active with passing of time. Coverage measurements in all of the monitoring locations found favorable coverage of over 95% after 12 weeks. The study results showed that the environmentally friendly functional concrete had outstanding environmental performance.

• Journal of Environmental Science International
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• v.16 no.10
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• pp.1203-1208
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• 2007

Feathers are produced in huge quantities as a waste product at commercial poultry processing plants. Since feathers are almost pure keratin protein, feather wastes represent an alternative to more expensive dietary ingredients for animal feedstuffs. Generally they become feather meal used as animal feed after undergoing physical and chemical treatments. These processes require significant energy and also cause environmental pollutions. Therefore, biodegradation of feather by microorganisms represents an alternative method to prevent environment contamination. The aim of this study was to investigate cultural conditions affecting keratinolytic protease production by Bacillus pumilus RS7. We also assessed the nutritive value of microbial and alkaline feather hydrolysates, The composition of optimal medium for the keratinolytic protease was fructose 0.05%, yeast extract 0.3%, NaCl 0.05%, K2HPO4 0.03%, KH2PO4 0.04% and MgCl2 6H2O 0.01%, respectively. The optimal temperature and initial pH was $30^{\circ}C$ and 9.0, respectively. The keratinolytic protease production under optimal condition reached a maximum after 18 h of cultivation. Total amino acid content of feather hydrolysates treated by NaOH and B. pumilius RS7 was $113.8\;{\mu}g/ml$ and $504.9\;{\mu}g/ml$, respectively. Essential amino acid content of feather hydrolysates treated by NaOH and B. pumilius RS7 was $47.2\;{\mu}g/ml$ and $334.0\;{\mu}g/ml$, respectively. Thus, feather hydrolysates have the potential for utilization as an ingredient in animal feed.

• Journal of Periodontal and Implant Science
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• v.31 no.3
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• pp.555-564
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• 2001

The purpose of this study is to evaluate the bioresorbability of Calcium Polyphosphate added with $Na_2O$ and chitosan. Though calcium phosphate ceramics meet some of the needs for bone replacement, they have some limitation of unresorbability and fibrous encapsulation without direct bone apposition during bone remodelling. To solve these problem, we developed a new ceramic, calcium polyphosphate(CPP), and report the biologic response to CPP in extraction sites of beagle dog. Porous CPP granules were prepared by condensation of anhydrous $Ca(H_2PO_4)_2$ to form non-crystalline $Ca(PO_3)_2$. CPP granules added with $Na_2O$ and chitosan were implanted in extraction sockets and histologic observation were performed at 12 weeks later. Histologic observation at 12 weeks revealed that CPP matrix were mingled with and directly apposed to new bone without any intervention of fibrous connective tissue. CPP granules added with chitosan were well adatped without any adverse tissue reaction and resorbed slowly and spontaneously. CPP granules added with $Na_2O$ and chitosan show multinucleated giant cells and osteoblast-like cells around grafted material and newly formed bone. This result revealed that CPP, regardless of its additive component, had a high affinity for bone and had been resorbed slowly. From this results, it was suggested that CPP is promising ceramic as a bone substitute and addition of $Na_2O$ and chitosan help biodegradation. In further study , it will be determined which concentration of $Na_2O$ help biodegradation and the other additive components increase the degradation rate.

• Journal of the Korean GEO-environmental Society
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• v.13 no.6
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• pp.83-90
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• 2012

Generally, the effect of the cement deep mixing method on the improvement of clay ground is far greater than the effect of physical improvement. Although it leads to great improvement strength in the initial stage, there are not many constructional precedents in Korea and it is hard to manage quality according to the cement-clay mixing method. In order to figure out the strength characteristics according to the mixing ratio of cement, sand, and clay and the improvement characteristics of weak ground according to the forms of the specimens to be improved, marine clay was used in this study to conduct the uniaxial compression test and soil bin model test. The test piece specimens for the uniaxial compression test were mixed with sand in a fixed ratio with the criterion of the water cement ratio. The cement was mixed with clay in the ratios of 10%, 20%, 30%, and 40% to the clay weight. The moisture content of the soil ground was made in the ratios of 40%, 60%, and 80%. The test piece specimens went through curing by moistening for 7, 14, and 28 days and underwent the uniaxial compression test according to the curing period. For the bearing test, the soil bin models were made and the ground improved in the Mat type was formed. After that, the bearing strength was compared in this study according to the improvement ratio and analyzed the intervening effect between the walls of the improved specimens.

• Korean Chemical Engineering Research
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• v.43 no.2
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• pp.318-323
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• 2005

Supercritical water oxidation (SCWO, P>221 bar, T>$374^{\circ}C$) is a promising method for the decomposition of refractory organic compounds. In this study, the SCWO of Ethylenediaminetetraacetic acid (EDTA) was carried out in a tubular-type continusous reactor system with an $H_2O_2$ oxidant at $387-500^{\circ}C$, 250 bar and residence time (RT) of 15.9-88.9 s. The decomposition efficiencies increased with increasing temperature and oxidant amount, while it was inversely proportional to feed flow rate. The decomposition efficiency of 99.6% was obtained at $500^{\circ}C$, 250 bar, oxidant amount of 400% and residence time of 40.1 s. The effect of temperature on the decomposition efficiency was more significant than that of oxidant amount. In the case of the decomposition efficiency of 5,000 mg/L of EDTA (3,063 mg/L as $COD_{Cr}$), the decompostion of 99% or higher was obtained at the condition of over 40.1 s (RT) and 200 stoichiometric % of $H_2O_2$ in the supercritical water of $500^{\circ}C$ and 250 bar.

• Korean Journal of Agricultural Science
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• v.6 no.1
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• pp.33-44
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• 1979

These studies were carried out to investigate the effects of liver-fluke infection on the reproductive disorder and milk and meat production in Korean Native Goat. A survey of infected goat in Chungnam district was conducted with 474 head by interdermal reaction and also a clinical observation was founded. A experiment was carried out to determine the effects of liver fluke extract on the reproductive disorder by subcutaneous injection and milk and meat yield were measured from treat ed goats. The results obtained were summarized as follows. 1. The rate of positive goat was 71.3% among the surveyed goat and the highest rate was appeared at 3~4 years old goats as 81.4%. 2. The treated goats lost condition and failed to thrive, and became progressive weaker. 3. In hemogram, observation, the rate of eosinophil leucocyte was increased significantly by extract treated. 4. It was appeared the pathogenic signs such symptoms poisoning liver function disturbance and reproductive disorder from treated goats with liver fluke extract. 5. In milking goat the milk yield droped significantly and fattening goat did not fatten. 6. The gross income value per capita was lower from infected goat and it was analysed more sensitivly in net income value.

• Journal of the Korean Geophysical Society
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• v.2 no.3
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• pp.179-190
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• 1999

The overall structural framework was studied using the regional 2D seismic data, followed by the sequence stratigraphic study on the 3D seismic and well- log data in the margin of the South Con Son basin of the South China Sea. This research contributes to delineate depositional stratigraphy, depositional environment and geologic history in the 3D seismic area of highly complicated faulting. Eight Miocene sequences were indicated on the 3D seismic and well-log data, in which the structural maps of each sequence boundary and the isochron maps for the corresponding sequence were made. The seismic facies were analyzed for each sequence volume and sequence boundary surface. The 3D seismic area is characterized by coal beds deposited in the transgression environment (transgression systems tract) and channel distributions just above the sequence boundaries. During the Early Miocene, the coals and thick shales deposited in the mangrove swamp representing the lower coastal plain environment. During the Mid to Late Miocene, thick clastic sediments deposited in the coastal to shallow shelf by regional subsidence and marine transgression. The isochron maps and structural patterns indicate that the sediments were transported from west to east or from northwest to southeast.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.4 no.4
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• pp.261-270
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• 1992

To investigate the flow patterns and diffusions in the Taean coastal waters of the eastern Yellow Sea, hydraulic and numerical experiments of tidal currents and diffusions of dye and cooling water were performed during spring tide along with field observations. Flow patterns obtained by the hydraulic and numerical experiments approximately coincide with those of the field observations. In the fold observations of tidal current, currents flow southwestward during the ebb tide, while currents flow northeastward during the flood tide. and the maximum velocity is 2.13 ㎧ toward WSW direction. The Eulerian diffusion coefficient estimated from field measmements of current is 7.82$\times$10$^{5}$ $\textrm{cm}^2$/s. Diffusion coefficients obtained from the area of dye plume in the model are given by the expression 0.18 $r^{4}$3/, and the coefficients have the range of 10$^{5}$ ~10$^{6}$ $\textrm{cm}^2$/s. These values are similar to the Eulerian diffusion coefficient estimated fram field measurements. Diffusion coefficients obtained in the hydraulic model are one to two orders higher than those obtained in the Onsan Bay in the eastern waters and two to three orders higher than those obtained in the Chinhae Bay in the southern waters of the Korean Peninsula. Diffusion patterns of cooling water by numerical experiments are similar to those of dye plume by hydraulic experiments. Both hydraulic and numerical experiment results of diffusions of dye plume and cooling water in the Taean coastal waters, have shown that the diffusion during the ebb tide is more prevalent than one during the flood tide.