• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.8
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• pp.1227-1232
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• 2003

The solvent extracts of the aerial part of Artemisia capillaris extracted by using several solvents with different polarities were prepared and their antimicrobial activities were examined. The antimicrobial activities and cell growth inhibitions were investigated to each strain with the different concentrations of the aerial part of Artemisia capillaris Acetone extract showed the highest antimicrobial activity. Minimum inhibitory concentrations (MIC) for each strain were appeared to 20 mg/mL at Staphylococcus aureus and Bacillus subtilis, 40 mg/mL at Vibrio parahaemolyticus, and 80 mg/mL at Salmonella tyhimurium. The cell growth inhibitions were shown on Staphylococcus aureus, Bacillus subtilis, Vibrio parahaemolyticus, and Salmonella typhimurium for 48 hours. The acetone extract was further fractionated sequentially with hexane, chloroform, ethyl acetate, and butanol for purifying crude acetone extract. The solvent fraction of hexane, chloroform, ethyl acetate, and butanol showed the different antimicrobial activity, respectively.

• Feed Journal
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• v.2 no.6
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• pp.58-64
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• 2004

생균제가 가축의 질병예방, 증체, 환경개선 등의 측면에서 항균성 물질에 버금가는 효과나 가능성이 공표(公表)됨에 따라, 항생물질 기능의 일부를 담당하면서 항균성 물질 대체제로서의 역할을 할 수 있다는 것은 이미 널리 알려진 주지(周知)의 사실이다. (중략)

• Journal of Food Hygiene and Safety
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• v.14 no.4
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• pp.346-351
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• 1999

To develop a lactic starter to produce antimicrobial substance for inhibiting the growth of a variety of foodborne spoilage bacteria in fermented foods, we investigated the anti-bacterial effect of the antibacterial substance, produced by Lactobacillus amylovorus IMC-1, against foodborne spoilage strains, and its sensitivity on the treatment of proteolytic enzymes. L. amylovorus IMC-1, which was isolated from a traditional cheese in Inner Mongolia, produced a maximum amount of antibacterial substance in the skim milk medium after 72 h incubation at 37$^{\circ}C$, and further incubation resulted in the same activity. The substance obtained from gel filtration inhibited all strains used such as Bacillus subtilis IFO 3025, Staphylococcus aureus IAM 1011, Listeria monocytogenes VTU 206, Escherichia coli RB, and Pseudomonas fragi IFO 3458 at the concentration of 20 units/ml. This substance was found to show bactericidal action against B. subtilis, E. coli, and Ps. fragi, and bacteriostatic activity against both Staph. aureus and L. monocytogenes. The bactericidal action was due to cellular Iysis. The substance is not organic acid, hydrogen peroxide and proteinaceous compound.

• The Korean Journal of Mycology
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• v.13 no.2
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• pp.89-97
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• 1985

To find antibacterial strains of the soil microorganisms in Korea, they were isolated from the soil samples of different locations and screened for antibacterial activity against several standard microorganisms. An isolate among them had antibacterial activities against gram-positive and gram-negative bacteria. The examination of its morphological, biochemical, cultural and physiological characteristics according to the International Streptomyces Project methods showed that it belongs to the genus Streptomyces. This strain appears to be a novel strain when it was compared with those species of the genus which have been so far reported. The antibiotic metabolite was produced in the submerged culture of the strain. This metabolite was extracted from the culture filtrate and purified by ion-exchange column chromatography. Physico-chemical properties of the antibacterial metabolite were characterized.

• Microbiology and Biotechnology Letters
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• v.35 no.3
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• pp.220-225
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• 2007

In order to develop a potent antidandruffy agent from medicinal plants, various extracts of medicinal plants and herbs were prepared and investigated its antifungal activity against Malassezia furfur. Among 85 kinds of the secondary screened medicinal plants and herbs, water extract of Chrysanthemum zawadskii had the highest antifungal activity against dandruffy-producing Malassezia furfur. The antifungal compound was also maximally extracted when the powder of Chrysanthemum zawadskii was treated with water at $50^{\circ}C$ for 6 hours. The antifungal compound from Chrysanthemum zawadskii was partially purified by silica gel column chromatography and the partial purified compound was soluble at $70^{\circ}C$ for 20 min and pH $3.0{\sim}9.0$.

• Microbiology and Biotechnology Letters
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• v.42 no.4
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• pp.393-401
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• 2014

A bacterium producing antimicrobial substance was isolated from cheonggukjang. The bacterium was identified as a strain of Bacillus subtilis by 16S rDNA sequencing and designated as Bacillus subtilis HH28. The antimicrobial substance produced from Bacillus subtilis HH28 was purified by 0-80% ammonium sulfate precipitation, DEAE-sepharose FF column chromatography, and Sephacryl S-200 HR gel chromatography. The molecular weight of the purified antimicrobial substance was estimated to be approximately 3,500 Da using Tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis and direct detection analysis. Antimicrobial substance from B. subtilis HH28 not only inhibited B. cereus, but also Listeria monocytogenes and Vibrio parahaemolyticus. The purified antimicrobial substance was stable at $40-80^{\circ}C$, and between pH 2 and 8. Antimicrobial activity of the purified substance was completely destroyed by treatment of protease, proteinase K, and pronase E, indicating that it is proteinaceous.

• Journal of dental hygiene science
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• v.15 no.2
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• pp.238-245
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• 2015

Dentinal hypersensitivity is a type of dental pain that occurs when various stimuli are applied to exposed dentin lesions. If the symptoms of dentinal hypersensitivity continue, the dentin is exposed and the hypersensitivity may become a periodontal disease or root caries due to bacterial infection. Therefore, the clinical goal of the present study is to reduce the pain of the patient suffering from dentinal hypersensitivity by developing antimicrobial hypersensitivity treatments and to improve oral hygiene. We prepared chlorhexidine, tetracycline, cetylpyridinium chloride, gallic acid loaded desensitizer by adding 0.1%, 0.5%, 1.0%, and 2.0% chlorhexidine, tetracycline, cetylpyridinium chloride, gallic acid to desensitizer (Micro Prime; Denville and Hurri Seal; Beutlich), and antibacterial effect, and cytotoxicity. The antibacterial test by using Staphylococcus aureus (SA. ATCC 6538, FDA 209) showed that the antibacterial effect of all experimental groups was significantly higher than that of control group (p<0.05). Cytotoxicity test by using agar diffusion assay indicated that Micro Prime showed mild toxicity, Hurri Seal showed severe toxicity and this cytotoxicity is supposed to be caused by one of desensitizer components showing weak antibacterial effect.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.1
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• pp.1-7
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• 2018

In this study, 1,2-hexanediol, ethylhexylglycerin, and glyceryl caprylate known as skin conditioning agents with antimicrobial activity in cosmetics were investigated for their antimicrobial activity against gram-positive and gram-negative bacteria when combining them with various ingredients in cosmetic formulations. Seven kinds of substances expected to improve the antibacterial activity in formulations were selected to blend with these three compounds.; 1,3-propanediol, 1,3-butanediol, glycerin, dipropylene glycol, niacinamide, EDTA-2Na, and ethanol. The checkerboard assay was conducted to confirm the antibacterial synergy of these seven substances and the three skin conditioning agents. Consequently, ethanol and EDTA-2Na were detected as the significant materials with synergistic effect as well as ethanol showed antibacterial synergy with all the three compounds. When seven of selected materials were mixed with glyceryl caprylate, there were synergistic or additive activity against S. aureus, gram-positive bacteria and it was suggested that the combination with 1,2-hexanediol and glyceryl caprylate was useful to control both gram-positive and gram-negative bacteria when there were four kinds of polyols. This study is meaningful in that it confirmed the antibacterial synergy of the skin conditioning agents with antimicrobial activity and the main raw materials frequently used in cosmetics, thereby contributing to prediction of the antibacterial activity of the skin conditioning agents in cosmetic formulations.

• Microbiology and Biotechnology Letters
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• v.41 no.1
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• pp.119-127
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• 2013

Twenty-three strains of Leuconostoc species and 45 strains of Weissella species inhibiting the growth of Lactobacillus sakei, one of the most populous lactic acid bacteria in over-ripened kimchi, were isolated from kimchi in our previous study. Among these hetero-fermentative 68 strains, Leuconostoc mesenteroides CK0128, Weissella cibaria CK0633, and W. cibaria KK0797 exhibited a relatively high survival rate in MRS medium, which was adjusted to pH 4.3 using an acid mixture consisting of acetic and lactic acids, and produced a large amount of exopolysaccharides. The culture supernatants of 3 strains were fractionated by a molecular weight cutter and lyophilized. The fractions with a molecular weight smaller than 3,000 Da showed antagonistic activity against Staphylococcus aureus and Lb. sakei. The anti-bacterial substances were very stable to heat treatments ($121^{\circ}C$, 15 min) and active at acidic conditions below pH 5. ${\alpha}$-Amylase, lipase, and proteolytic enzymes (proteinase K and pepsin) did not affect their activities. These non-proteinaceous anti-bacterial substances inhibited the growth of several food pathogens.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.60-60
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• 1993

새로운 항생물질을 개발하기 위하여 토양으로부터 분리한 균주를 액체배지에서 배양하여 그 배양액을 여러 검정균에 대하여 종이디스크법으로 항균효력을 측정하였다. 그 결과 G(＋), G(－)균에 강한 항균효력을 보인 토양균 SNUS 9101-55와 C. aibicans와 같은 진균류에 항균력을 보인 토양균 SN-US 9101-68을 선택하여 각각의 배양액에서 항생물질을 분리하고, 분리한 항생물질의 구조를 규명하고자 하였다. 토양균 SNUS 9101-55의 배양액으로부터 항생물질을 분리하기 위하여 양이온 교환수지 관 크로마토그래피와 셀룰로오스 관 크로마토그래피를 수행하여 시료 AMJ-I-212-B를 얻었다. 시료 AMJ-I-212-B의 IR, $^1$H-NMR, FAB-MS 스펙트럼을 얻어 분리한 항생물질의 구조를 분석하고 Sakaguchi test와 같은 정색반응을 통하여 그 발색단을 동정하여 이 항생물질의 구조가 스트렙토마이신과 동일하다는 것을 확인하였다.