Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Production and Characteristics of Antidandruffy Compound from Chrysanthemum zawadskii

구절초(Chrysanthemum zawadskii)로부터 비듬균에 대한 항균물질의 생산 및 특성

  • Published : 2007.09.28
Download PDF
⟨ Previous Next ⟩

Abstract

In order to develop a potent antidandruffy agent from medicinal plants, various extracts of medicinal plants and herbs were prepared and investigated its antifungal activity against Malassezia furfur. Among 85 kinds of the secondary screened medicinal plants and herbs, water extract of Chrysanthemum zawadskii had the highest antifungal activity against dandruffy-producing Malassezia furfur. The antifungal compound was also maximally extracted when the powder of Chrysanthemum zawadskii was treated with water at $50^{\circ}C$ for 6 hours. The antifungal compound from Chrysanthemum zawadskii was partially purified by silica gel column chromatography and the partial purified compound was soluble at $70^{\circ}C$ for 20 min and pH $3.0{\sim}9.0$.

각종 약용 식물과 허브의 물 추출물을 제조하여 비듬균에 대한 항균활성을 조사한 결과 구절초(Chrysanthemum zawadskii)의 물 추출물이 7 mm의 생육저해환을 보여 가장 높은 항균활성을 보였다. 이 항균물질은 구절초 분말을 1:20으로 물에 현탁시킨 후 $50^{\circ}C$에서 6시간 진탕시켰을 때 가장 많이 추출되었고, 이 항균물질을 Diaion HP 20으로 크로마토그라피를 실시하여 부분 정제한 결과 정제물질은 물과 에탄올에 비교적 잘 녹았고 pH 3.0에서 9.0까지 안정하였다. 또한 구절초 물 추출물을 30% 첨가하여 제조한 시제품이 가장 항균활성이 우수하였고 첨가 농도가 높아질수록 굴절률은 비슷하였으나 pH와 점도는 오히려 낮아지는 경향을 보였다.

Keywords

References

  1. A.O.A.C. 1995. Official methods of analysis. 15th edition, Edited by Kenneth Helrich, Association of Official Analysis Chemist, Virginia, U.S.A
  2. Choi, I. 2003. Antimicrobial activity of Rhus javanica extracts against animal husbandry disease-related bacteria. J. Kor. Soc. Food Sci. Nutr. 32: 1214-1220 https://doi.org/10.3746/jkfn.2003.32.8.1214
  3. Dermatological Science, Korean Dermatological Association. 2001. Yeomungak. pp. 325
  4. Dong, S. and S. H. Jeng. 2004. Antioxidant activities of clove by extraction solvent. J. Kor. Soc. Food Sci. Nutr. 33: 609-613 https://doi.org/10.3746/jkfn.2004.33.4.609
  5. Ham, S. S., D. H. Oh, J. K. Hong, and J. H. Lee. 1997. Antimutagenic effects of juices from edible Korean wild herbs. J. Food Sci. Nutr. 2: 155-161
  6. Hyeon, J. J., D. W. Kim., J. B. Jun, and S. L. Chung. 1993. Statistical and mycologic studies on dandruff. Kor. J. Dermatol. 31: 164-174
  7. Jun, S., K. Goto, F. Nanjo, S. Kawai, and K. Murata. 2000. Antifungal activity of plant extracts against Arthrinium sacchari and Chaetomium funicola. J. Biosci Bioeng. 90: 442-446 https://doi.org/10.1016/S1389-1723(01)80016-5
  8. Kim, S. T., K. T. Lee, and T. J. Min. 2003. Characteristics of antimicrobial activities for the human pathogenic microorganism by extracts from Korean mushrooms. Kor. J. Mycol. 31: 67-76 https://doi.org/10.4489/KJM.2003.31.2.067
  9. Lee, K. S., J. C. Lee, K. H. Han, and M. J. Oh. 1999. Antimicrobial activities of extracts of Perilla frutescens Briton var. acuta Kudo on food spoilage or foodbome disease microorganisms. Kor. Soc. Food Preserv. 6: 239-244
  10. McGinley, K. J., J. J. Leyden, and R. R. Marples. 1979. Quantitative microbiology of the scalp in non-dandruff, dandruff and seborrheic dermatitis. J. Invest. Dermatol. 64: 401-405 https://doi.org/10.1111/1523-1747.ep12512335
  11. Oh, M. H. and H. J. Whang. 2003. Chemical composition of server herb plants. Kor. J. Food Sci. Techol. 35: 1-6
  12. Park, J. S. 2000. Isolation and purification of antimicrobial active substances from aloe vera peel(II). Kor. J. Food Nutr. 13: 146-151
  13. Roberts, S. B. 1969. Pityrosporum orbiculare incidence and disribution in clinically normal skin. Br. J. Dermatol. 81: 264-269 https://doi.org/10.1111/j.1365-2133.1969.tb13978.x
  14. Ryoo, J. W. and B. C. Chao 1998. Mineral content and anti oxidative acitity in some herb plants. Kor. J. Med. Crop Sci. 6: 28-32
  15. Sin, D. H. 2003. Screening and application of antibacterial and antioxidant compound from natural sources. Food Science and Industry. 36: 81-88