• Title/Summary/Keyword: 한국생명공학연구원

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Draft genome sequence of Bacteroides sp. KGMB 02408 isolated from a healthy Korean feces (건강한 한국인 분변으로부터 분리된 Bacteroides sp. KGMB 02408 균주의 유전체 염기서열 초안)

  • Yu, Seung Yeob;Kim, Ji-Sun;Oh, Byeong Seob;Ryu, Seoung Woo;Park, Seung-Hwan;Kang, Se Won;Park, Jam-Eon;Choi, Seung-Hyeon;Han, Kook-Il;Lee, Keun Chul;Eom, Mi Kyung;Suh, Min Kuk;Kim, Han Sol;Lee, Dong Ho;Yoon, Hyuk;Kim, Byung-Yong;Lee, Je Hee;Lee, Jung-Sook;Lee, Ju Huck
    • Korean Journal of Microbiology
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    • v.55 no.3
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    • pp.296-299
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    • 2019
  • The genus of Bacteroides has been isolated from vertebrate animal feces. Bacteroides sp. KGMB 02408 was isolated from fecal samples obtained from a healthy Korean. The wholegenome sequence of Bacteroides sp. KGMB 02408 was analyzed using the PacBio Sequel platform. The genome comprises a 5,771,427 bp chromosome with a G + C content of 39.50%, 5,005 total genes, 18 rRNA genes, and 74 tRNA genes. Furthermore, we found that strain KGMB 02408 had some genes for oxidoreductases and menaquinone biosynthesis in its genome based on the result of genome analysis.

Draft genome sequence of Senegalimassilia sp. KGMB 04484 isolated from healthy Korean human feces (건강한 한국인 분변으로부터 분리된 Senegalimassilia sp. KGMB 04484 균주의 유전체 염기서열 초안)

  • Han, Kook-Il;Kang, Se Won;Kim, Ji-Sun;Lee, Keun Chul;Eom, Mi Kyung;Suh, Min Kuk;Kim, Han Sol;Park, Seung-Hwan;Lee, Ju Huck;Park, Jam-Eon;Oh, Byeong Seob;Yu, Seung Yeob;Choi, Seung-Hyeon;Lee, Dong Ho;Yoon, Hyuk;Kim, Byung-Yong;Lee, Je Hee;Lee, Jung-Sook
    • Korean Journal of Microbiology
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    • v.55 no.2
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    • pp.160-163
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    • 2019
  • Senegalimassilia sp. KGMB 04484 was isolated from fecal samples obtained from a healthy Korean. The whole-genome sequence of Senegalimassilia sp. KGMB 04484 was analyzed using the PacBio Sequel platform. The genome comprises a 2,748,041 bp chromosome with a G+C content of 61.18%, 2,300 total genes, 2,139 protein-coding gene, 21 rRNA genes, and 51 tRNA genes. Also, we found that strain KGMB 04484 had some genes for hydrolysis enzyme, fatty acid biosynthesis and metabolism in its genome based on the result of genome analysis. Those genes of KGMB 04484 may be related to regulation of human health and digest.

Draft genome sequence of Olsenella sp. KGMB 04489 isolated from healthy Korean human feces (건강한 한국인 분변으로부터 분리된 Olsenella sp. KGMB 04489 균주의 유전체 염기서열 초안)

  • Han, Kook-Il;Kang, Se Won;Kim, Ji-Sun;Lee, Keun Chul;Eom, Mi Kyung;Suh, Min Kuk;Park, Seung-Hwan;Lee, Ju Huck;Park, Jam-Eon;Oh, Byeong Seob;Yu, Seung Yeob;Choi, Seung-Hyeon;Lee, Dong Ho;Yoon, Hyuk;Kim, Byung-Yong;Yang, Seung-Jo;Lee, Jung-Sook
    • Korean Journal of Microbiology
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    • v.54 no.4
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    • pp.456-459
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    • 2018
  • The genus of Olsenella has been isolated from vertebrate animal mouth, rumen, and feces. Olsenella sp. KGMB 04489 was isolated from fecal samples obtained from a healthy Korean. The whole-genome sequence of Olsenella sp. KGMB 04489 was analyzed using the PacBio Sequel platform. The genome comprises a 2,108,034 bp chromosome with a G + C content of 65.50%, 1,838 total genes, 13 rRNA genes, and 52 tRNA genes. Also, we found that strain KGMB 04489 had some genes for hydrolysis enzymes, and antibiotic biosynthesis and resistance in its genome based on the result of genome analysis.

Infrastructure Services of KRIB Herbarium in IBMRC (KRIBB) (한국생명공학연구원 해외생물소재센터 식물표본관(KRIB)의 인프라 서비스)

  • Nam, Bo-Mi;Paik, Jin-Hyub;Lee, Changyoung;Choi, Sangho
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.42-42
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    • 2019
  • 한국생명공학연구원은 1985년 설립된 이후로 현재까지 국가적 연구개발 수요에 대응하여 바이오 분야의 기초 원천 연구, 인프라 구축 및 운영을 통하여 우리나라 생명과학기술 발전에 힘써왔다. 특히 2006년부터는 해외생물자원의 중요성에 주목하여 차세대 국가 핵심 전략 BT산업의 필수 원자재인 생물소재의 범지구적 확보, 보존 및 관리를 위하여 해외생물소재센터를 설치하였다. 이후 차례로 중국, 코스타리카, 인도네시아, 베트남의 4개 거점 센터를 개소 및 운영하여 권역별 해외생물소재의 공동연구 및 국제협력 네트워크를 구축하고 효과적인 소재 확보 및 관리를 도모하고 있다. 이와 같이 확보된 소재는 36개국에서 채집된 약 318과 3,406속의 36,500여점 (2019년 9월 기준)에 달하고 소재의 확증표본은 해외생물소재센터 식물표본관(KRIB)에 보관 중이다. KRIB표본관은 2000년 Index Herbariorum에 등록되었으며, 약 80,000여점의 건조표본을 소장하고 있다. 분류군수는 과별로 콩과>국화과>꼭두서니과 순으로 많았으며, 국가별로는 중국>베트남>코스타리카 순으로 조사되었다. 한국생명공학연구원 해외생물소재센터 및 식물표본관은 소재의 정확한 동정을 위한 분류학적 시설로서의 역할뿐 아니라 누구에게나 개방된 홈페이지를 통하여 해외소재의 표본정보, 표본이미지 및 민속학적 정보와 활성 등 가치 있는 정보를 제공하여 연구자들에게 다양한 활용지원 서비스를 제공하고 있다.

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International Biological Material Procurement and Utilization Research (해외생물소재 확보 및 활용 연구)

  • Yun, Narae;Nam, Bo-Mi;Lee, Changyoung;Kim, Soo-Yong;Paik, Jin-Hyup;Choi, Sang-Ho
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.99-99
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    • 2019
  • 한국생명공학연구원 해외생물소재센터는 해외생물소재 확보 및 활용연구를 통하여 차세대 국가핵심전략 BT산업의 필수원자재인 생물소재의 범지구적 확보와 보존 관리의 임무를 수행하고 있다. 이를 위해 권역별 해외생물소재 공동연구센터(중국, 코스타리카, 인도네시아, 베트남) 및 37개국과의 국제협력 네트워크를 구축하고 있다. 세계적 수준의 생물소재 국가 인프라 구축을 목표로 국내 산 학 연 연구자들에게 다양한 해외생물소재 공급을 통한 지속 가능한 바이오 경제 기반 구축을 지원하고 있다. 해외생물소재센터에서는 2006년부터 특히 천연물 의약품 개발 분야에 많이 사용 되고 있는 해외 식물소재를 지속적으로 확보하고 있으며, 확보된 식물소재의 추출물 제조 및 추출물 은행을 구축, 이에 대한 생물활성 평가를 실시하며 연구자들에게 기초 자료로 제공하고 있다. 현재까지 총 36,500종의 해외식물소재를 확보하였으며, 추출물 약 320만점을 확보하였다. 확보된 소재의 분양활동을 통해 산 학 연 연구자들에게 180만여 점을 분양하였으며, 이를 활용한 다수의 논문과 특허를 획득하였고 중대형 기술이전을 실시하였다. 해외생물소재센터는 홈페이지 기반 분양 신청 시스템[https://www.ibmrc.re.kr]을 통하여 해외식물소재(Powder 와 Extact 형태) 분양서비스를 제공하고 있다. 또한 소재가치제고 연구를 통한 산업화 지원과 해외생물소재의 표본정보 서비스, 해외거점센터를 활용한 현지정보 제공 서비스를 지원하고 있다.

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Draft genome sequence of Ruminococcus sp. KGMB03662 isolated from healthy Korean human feces (건강한 한국인 분변으로부터 분리된 Ruminococcus sp. KGMB03662 균주의 유전체 염기서열 초안)

  • Han, Kook-Il;Kang, Se Won;Eom, Mi Kyung;Kim, Ji-Sun;Lee, Keun Chul;Suh, Min Kuk;Kim, Han Sol;Park, Seung-Hwan;Lee, Ju Huck;Park, Jam-Eon;Oh, Byeong Seob;Ryu, Seoung Woo;Yu, Seung Yeob;Choi, Seung-Hyeon;Lee, Dong Ho;Yoon, Hyuk;Kim, Byung-Yong;Lee, Je Hee;Lee, Jung-Sook
    • Korean Journal of Microbiology
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    • v.55 no.3
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    • pp.274-277
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    • 2019
  • Ruminococcus sp. KGMB03662 was isolated from fecal samples obtained from a healthy Korean. The whole-genome sequence of Ruminococcus sp. KGMB03662 was analyzed using the PacBio Sequel platform. The genome comprises a 2,707,502 bp chromosome with a G + C content of 43.09%, 2,484 total genes, 2,367 protein-coding gene, 14 rRNA genes, and 53 tRNA genes. In the draft genome, genes involved in the hydrolysis enzyme, fatty acid biosynthesis, fatty acid metabolite, antibiotic biosynthesis, and antibiotic resistance have been identified. Those genes of KGMB03662 may be related to the regulation of human health and disease.

Complete genome sequence of Lachnospiraceae bacterium KGMB03038 (=KCTC 15821) isolated from healthy Korean feces (건강한 한국인 분변으로부터 분리된 Lachnospiraceae bacterium KGMB03038 (=KCTC 15821) 균주의 유전체 염기서열 초안)

  • Kim, Ji-Sun;Kang, Se Won;Han, Kook-Il;Lee, Keun Chul;Eom, Mi Kyung;Suh, Min Kuk;Kim, Han Sol;Lee, Ju Huck;Park, Seung-Hwan;Park, Jam-Eon;Oh, Byeong Seob;Yu, Seung Yeob;Choi, Seung-Hyeon;Ryu, Seoung Woo;Lee, Dong Ho;Yoon, Hyuk;Kim, Byung-Yong;Lee, Je Hee;Lee, Jung-Sook
    • Korean Journal of Microbiology
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    • v.55 no.3
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    • pp.289-292
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    • 2019
  • Lachnospiraceae bacterium KGMB03038 (=KCTC 15821) belonging to the class Clostridia in phylum Firmicutes, was isolated from a stool sample of a healthy Korean. Herein, we report the complete genome sequence of strain KGMB03038 analyzed using the PacBio Sequel platform. The genome comprises of 3,334,474 bp with G + C content of 47.8%, which includes 3,099 predicted protein-coding genes, 12 ribosomal RNAs, 54 transfer RNAs, and 4 ncRNAs. Genome analysis revealed that strain KGMB03038 possesses a number of genes involved in hydrolysis of carbohydrates, including mono-, di-, and oligo-saccharides, and biosynthesis of various amino acids.

Screening of Gamma Radiation-Induced Pathogen Resistance Rice Lines against Xanthomonas oryzae pv. oryzae (방사선을 이용한 벼 흰잎마름병 저항성 돌연변이 벼 계통의 선발)

  • Lim, Chan Ju;Lee, Ha Yeon;Kim, Woong Bom;Ahmad, Raza;Moon, Jae Sun;Kim, Dong Sub;Kwon, Suk-Yoon
    • Journal of Radiation Industry
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    • v.4 no.3
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    • pp.209-213
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    • 2010
  • Bacterial blight is one of the most serious diseases of rice (Oryza sativa L.), and it has been known that Xanthomonas oryzae pv. oryzae (Xoo) causes this disease symptom. To develop resistance rice cultivars against Xoo, 3,000 lines of $M_3$ mutants, which were irradiated with gamma ray, were tested by 'scissor-dip method' primarily, and 191 putative resistant lines were selected. In $M_4$ generation, these lines were screened again with various ways such as measuring of symptom of bacterial blight in leaf, number of tiller, fresh weight, and phenotypic segregation ratio in next generation. Finally, six resistance lines were selected. RT-PCR analysis revealed that these lines displayed high level of R-genes such as Xa21, Pi36, and Pi-ta. These results indicate that mutations by gamma ray cause disruptions of regulatory signal transduction systems of these R-genes. Furthermore, these selected mutants could be useful for the development of rice cultivar resistant to Xoo.

Development of Freeze-dried Miyeokguk, Korean Seaweed Soup, as a Space Food Sterilized by Irradiation (동결건조 미역국을 이용한 방사선 멸균 우주식품 개발)

  • Lee, Ju-Yeon;Song, Beom-Seok;Park, Jin-Gyu;Cho, Won-Jun;Kim, Jae-Hun;Yoon, Yo-han;Choi, Jong-il;Lee, Ju-Woon
    • Journal of Radiation Industry
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    • v.4 no.1
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    • pp.59-64
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    • 2010
  • This study was conducted to evaluate the microbial (Total aerobic count, Coliform, Coagulase positive Staphylococci, Salmonella, Yeast/Mold, Escherichia coli, Bacillus cereus), Hunter'color values ($L^*$, $a^*$, $b^*$) and organoleptic quality of freeze-dried Miyeokguk, Korean seaweed soup in space food. Bacillus cereus was 1 log level while not detected in the sample irradiated at 10 kGy. Hunter's color values of the samples irradiated less than 10 kGy was not significantly different (p>0.05). The sensory evaluation result showed that the preference scores in all the sensory properties decreased when it was irradiated over 10 kGy, but sensory score of less than 10 kGy samples was similar in all terms. Therefore, it was considered that gamma irradiation at 10 kGy was enough to sterilize the freeze-dried Miyeokguk without deterioration of sensory quality.