• Progress in Medical Physics
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• v.13 no.2
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• pp.62-68
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• 2002

A muiltileaf collimator (MLC) is used as a replacement for conventional blocks. The MLC, however may not be appropriate for a fine field shaping. For the fine field shaping, conventional block can be added under the MLC. But it may significantly affect on the dosimetric characteristics such as surface dose of skin, buildup region and percent depth doses. We performed the study to evaluate the surface dose and the maximum depth dose using MLC conjunction with conventional blocks for various field sizes and energies. We confirmed the surface dose was increased by using the additional conventional block under the MLC ranging from 10 to 35.6％ according to various field sizes and radiation beam energies. The surface dose was effectively reduced by application of 2 or 3 m thickness of lead plate as electron filter.

• Journal of the korean veterinary medical association
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• v.38 no.4
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• pp.376-382
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• 2002

호주의 많은 소동물 임상수의사들이 최근에 관심을 갖고 있는 부분은 개의 아토피(Canine Atopy)에서의 알러지-특이성(Allergen-specific)IgE에 쓰이는 혈청 ELISA (Enzyme-Linked Immuno-Sorbent Assay, 효소결합면역흡착검사)테스트의 활용 가능성에 관한 것이다. 희망사항은 개 아토피의 진단과 치료에 있어서 도움이 되도록 접근할 수 있는 시험(Accessible test)일 것이다. 아토피(Atopy)는 진단과 치료를 실패시키는 질병이며, 새로운 ELISA라도 이 문제를 쉽게 해결하여 주지 못한다. 개의 아토피에서 알려진(Allergens)들을 분석하는 데 쓰이는 혈청 ELISA처럼 아주 정확하게 특성화되어져야 한다. 해당 개는 아토피성(Atopic)으로서 진단되어져야만 되고 그 다음에 치료에 지침이 되도록 개별 알러진 동정(Individual allergen identification)용 ELISA로 개의 혈액을 스크린하는데 적합하여야 한다. ELISA와 피내접종시험(Internal testing)방법은 상관도가 낮고 그렇기 때문에 어떤 시험이 더 신뢰할 수 있는 것일까? 이러한 사실은 논쟁의 여지가 있으며 이상의 2가지 시험들이 옳다면 각각의 상응하는 각각 다른 아형(Subtypes)의 아토피라고 볼 수있다. 아마도 알러진들은 경피적으로 흡수되어 아토피성피부염으로 되어진다. 그것은 인체에서 건초열(Hay fever)이나 천식(Asthma)과 같이 흡입에 의해 일으키는 유사한 증후군동종 (Syndrome akin)과 같은 접근의 초기양식(Primary mode of access)으로부터 생기는 다른 유사한 질병과정이 되어질 수도 있다. 이러한 2가지 증후군은 함께 병발하는 경우가 있다. 의학에서는 피부 반점시험방법의 예비조사연구(Preliminary research with skin patch testing in medicine)는 아토피성 피부염을 야기하는 알러진들의 경피흡수경로의 개념을 지지해준다. 흡인 알러진들을 사용한 개들에서의 도전 시험(Challenge tests)들은 거의 대부분이 비-피부학적 임상증상들을 발현하였다. 모든 증거가 분명히 나타나도록 미래의 조사연구를 인도해 주는 게 필요하며 개 아토피의 정의와 진단을 둘러싼 수수께끼를 해결하는데 도움이 될 것이다.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.49 no.3
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• pp.231-245
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• 2023

In the cosmetics industry, it is important to develop new materials for functional cosmetics such as whitening, wrinkles, anti-oxidation, and anti-aging, as well as technology to increase absorption when applied to the skin. Therefore, in this study, we tried to optimize the nanoemulsion formulation by utilizing response surface methodology (RSM), an experimental design method. A nanoemulsion was prepared by a high-pressure emulsification method using Glabridin as an active ingredient, and finally, the optimized skin absorption rate of the nanoemulsion was evaluated. Nanoemulsions were prepared by varying the surfactant content, cholesterol content, oil content, polyol content, high-pressure homogenization pressure, and cycling number of high-pressure homogenization as RSM factors. Among them, surfactant content, oil content, high-pressure homogenization pressure, and cycling number of high-pressure homogenization, which are factors that have the greatest influence on particle size, were used as independent variables, and particle size and skin absorption rate of nanoemulsion were used as response variables. A total of 29 experiments were conducted at random, including 5 repetitions of the center point, and the particle size and skin absorption of the prepared nanoemulsion were measured. Based on the results, the formulation with the minimum particle size and maximum skin absorption was optimized, and the surfactant content of 5.0 wt%, oil content of 2.0 wt%, high-pressure homogenization pressure of 1,000 bar, and the cycling number of high-pressure homogenization of 4 pass were derived as the optimal conditions. As the physical properties of the nanoemulsion prepared under optimal conditions, the particle size was 111.6 ± 0.2 nm, the PDI was 0.247 ± 0.014, and the zeta potential was -56.7 ± 1.2 mV. The skin absorption rate of the nanoemulsion was compared with emulsion as a control. As a result of the nanoemulsion and general emulsion skin absorption test, the cumulative absorption of the nanoemulsion was 79.53 ± 0.23%, and the cumulative absorption of the emulsion as a control was 66.54 ± 1.45% after 24 h, which was 13% higher than the emulsion.

• Applied Microscopy
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• v.37 no.3
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• pp.157-166
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• 2007

This experiment was performed to evaluate the ultrastructural responses of the absorptive cells in the appendix of the mouse, inoculated with Ehrlich carcinoma cells in the inguinal area, following administration of BCG (Bacillus Calmette-Guerin). Healthy adult ICR mice weighing 25 gm each were divided into normal and experimental groups (experimental control group and BCG treated group). In the experimental groups, each mouse was inoculated with $1{\time}10^7$ Ehrlich carcinoma cells subcutaneously in the inguinal area. From next day after inoculations, 0.5mL of saline or BCG (0.5 mL/25gm B.W.: $0.03{\times}10^8{\sim}0.32{\times}10^8CFU$) were injected subcutaneously to the animals every other day. The day following the last injection, each mouse was sacrificed. Pieces of the tissue were taken from the appendix, prefixed with 2.5% glutaraldehyde-1.5% paraformaldehyde solution, followed by post-fixation with 1% osmium tetroxide solution. The ultrathin sections were stained with uranyl acetate and lead citrate. In the normal control, experimental control and BCG treated mice, general morphology of the absorptive cells of appendix were similar. But myelin figures and intramitochondrial dense granules were more frequently observed in the absorptive cells of BCG treated mice than normal control ones. Above results show that BCG did show slight ultrastructural alterations in the absorptive cell of the appendix. These results that BCG may slightly suppress function of the absorptive cells of the appendix.

• The Journal of the Convergence on Culture Technology
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• v.5 no.3
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• pp.271-282
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• 2019

This study was conducted to evaluate physiological activity of Robinia pseudo-acacia leaf and its skin penetration using polymer micelles and skin penetrating peptide. After extraction with Robinia pseudo-acacia using the ethanol and distilled water, various physiological activities were examined. The total concentration of polyphenol compounds was determined to be 47.42 mg/g (ethanol extract), 56.88 mg/g (hydrothermal extract) and DPPH radical scavenging ability at $1,000{\mu}g/mL$ was 44.24% in ethanol extract and it is higher than value(41.50%) in hydrothermal extract. The elastase inhibitory assay showed concentration dependence and elastase inhibition of Robinia pseudo acacia leaf ethanol extract was 54.09%, which was the highest at $500{\mu}g/mL$. In the SOD-like experiments, the concentration-dependent results were showed and the SOD-like activity of the Robinia pseudo-acacia leaf ethanol extract was higher than that of the Robinia pseudo acacia leaf hydrothermal extract at all concentrations. At a concentration of $500{\mu}g/mL$, Robinia pseudo acacia leaf ethanol extract showed the highest SOD-like activity of 76.41%. The tyrosinase inhibition at $20{\mu}g/mL$ was determined to be 56.47% (ethanol extract), 23.05% (hydrothermal extract). In the antimicrobial experiments, the hydrothermal extract had no effect, but ethanol extract represented maximum clear zone of 11.00 mm in Propionbacterium acnes strain and maximum clear zone of 10.50 mm. in Bacillus subtilis strain. To solve the problem of insolubility and to improve skin penetration, PCL-PEG polymer micelles containing Robinia pseudo-acacia leaf ethanol extracts and 1.0% cell permeable peptide, hexa-D-arginine (R6) were successfully prepared with particle size of 108.23 and 126.47 nm and excellent skin permeation effects could be showed.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.12 no.3
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• pp.409-426
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• 2001

Absorbed power of the human head radiated from a 900 MHz portable phone and temperature rise are computed using FDTD(Finite-Difference Time-Domain) method. For this computation the 5 layered media for the human head modeling and the monopole antenna attached to metallic box for the portable phone are used. To reflect the real circumstances typical sizes of human heads and portable phones are considered in the calculation. The length of monopole antenna is 8.15 cm, and the output power of a phone is 600 mW. Under the predetermined model the distribution of 1 g, 10 g averaged SAR and temperature rise rate over the human head are calculated, from which it was found that the position of maximum SAR is near at the head skin surface, not deep places far into the head. The position of the highest temperature is located far from the head skin more than that of the maximum SAR occured. The averaged SAR and temperature along the distance between the head and phone are calculated according to seperation distance between the head and phone.

• Polymer(Korea)
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• v.28 no.6
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• pp.478-486
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• 2004

Small intestinal submucosa (SIS) is consisted with collagen and glycosaminoglycan as well as some growth factors which can stimulate cell activity. Recently, it has been recognized that SIS has been successfully examined in the bio-medical application as biomaterials without xenograft immune-rejection response. We prepared native SIS sheets and acid treated SIS sheets by acetic acid with 1 or 5-layered sheets, respectively. The water uptake ability of native and acid treated SIS sheets was examined to evaluate the possibility as wound dressings. Morphologies of SIS sheets were characterized by SEM and the effects of various buffer solutions and different pH solutions on the water uptake ability were observed for 16 days. We observed that the acid treated SIS sheets had higher water uptake ability than native SIS sheets. Also, the water uptake ability of these was slightly higher in various buffers than distilled water. In conclusion, this study suggests that native and acid treated SIS sheets could be useful for the applications of wound dressing and biodegradable injectable materials.

• The Journal of Korean Society for Radiation Therapy
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• v.34
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• pp.7-12
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• 2022

Purpose: When it is difficult to secure the skin dose when treating Irregularly Shaped Skin Surface such as the nose where it is difficult to apply a bolus, increase the skin dose with a treatment plan that combines the IMRT (Intensity Modulated Radiation Therapy) delivery technique and FFF (Flattening Filter Free), It was tried to find out whether or not through the phantom experiment. Materials & Methods: Based on the 6MV-FF (Flattening Filter) and VMAT (Volumetric-Modulated Arc Therapy) treatment plans, which are the most commonly used treatment plans for head and neck cancer, A comparison group was created by combining VMAT and IMRT, FF and FFF, and the presence or absence of 5 mm bolus application. A virtual target was created on the Rando Phantom's nose, and a virtual bolus of 5 mm was applied assuming full contact on the Rando Phantom's nose. Five measurement points were determined based on the phantom's nose, and the absorbed dose was measured by irradiating each treatment plan 3 times per treatment plan according to the treatment technique and whether or not the bolus was applied. Result: The difference in skin dose in FF vs FFF increased in the case of FFF in VMAT bolus off, and there was no difference in case of IMRT bolus off. In VMAT bolus 5 mm and IMRT bolus 5 mm, it was confirmed that the skin dose was rather decreased in FFF. The difference in skin dose between VMAT and IMRT increased only in the case of FFF bolus off, and there was no statistical difference in the rest. For the difference in skin dose between bolus off vs bolus 5 mm, it was confirmed that the skin dose increased at bolus 5 mm, except for the case of using IMRT FFF. The treatment plan combining IMRT and FFF did not find any statistically significant difference as a result of analyzing the measured values of the treatment plan skin dose applied with a 5 mm bolus using the commonly used VMAT and FF. Therefore, it is thought that by using IMRT_FFF, it is possible to deliver a skin dose similar to that of applying a 5 mm bolus to VMAT_FF, which can be useful for patients who need a high skin dose but have difficulty applying a bolus. Conclusion: For patients who find it difficult to apply bolus, an increase in skin dose can be expected with a treatment plan that properly combines IMRT and FFF compared to VMAT and FF.

• Journal of the Korean Applied Science and Technology
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• v.30 no.1
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• pp.116-126
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• 2013

Glycol ethers are a group of solvents based on alkyl ethers of ethylene glycol commonly used in paints. These solvents typically have a higher boiling point, together with the favorable solvent properties of lower-molecular weight ethers and alcohols. The word "Glycol ethers" was registered as a United States trademark by Union Carbide Corp. Typically, glycol ethers are found in pharmaceuticals, sunscreens, cosmetics, inks, dyes and water based paints. On the other hand, glycol ethers are used in degreasers, cleaners, aerosol paints and adhesives. Most glycol ethers are relatively water soluble, biodegradable and only a few are considered toxic. Therefore, they are unlikely to pose an adverse risk to the environment. Recent study suggests that occupational exposure to glycol ethers is related to low motile sperm count in men, but the finding has been disputed by others. In this study, skin permeation of 3 types glycol ethers were studied in vitro using matrix such as solvent and detergent. The absorption of glycol ethers[methyl glycol ethers(MC), ethyl glycol ethers(EC) and butyl glycol ethers(BC)] has been measured in vitro through rat skin. Epidermal membranes were set up in Franz diffusion cells and their permeability to PBS measured to establish the integrity of the skin before the glycol ethers were applied to the epidermal surface. Absorption rates for each glycol ethers were determined and permeability assessment made to quantify any irreversible alterations in barrier function due to contact with the esters. Types of glycol ethers in vitro experimental results on MC> EC> BC quickly appeared in the following order: skin permeation was beneficial to the skin permeation small molecular weight, the difference in chemical structure, such as hydrophilic, because with the partition coefficient and solubility mechanisms and passive diffusion to increase the speed at which transmission is considered.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.2
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• pp.175-184
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• 2019

The epidermal growth factor (EGF) has a intrinsic function of inducing growth and proliferation of cells through interacting with cell membrane receptors in human epidermis and dermis layer. These functions of EGF are used as a main ingredient for wound healing medicines and anti-aging cosmetics. As a cosmetic ingredient, the EGF has a problem in exhibiting its natural efficacy due to the lack of the ability to penetrate through the stratum corneum, which is known as the skin barrier. In this study, a recombinant human epidermal growth factor ($MTD_{151}-EGF$) fused with the macromolecule transduction domain $(MTD)_{151}$ with the skin penetration ability was developed to improve the skin penetration efficiency of the EGF. Expression of $MTD_{151}-EGF$ was performed in E. coli transformed with a vector encoding the $MTD_{151}-EGF$ gene and then purified. The purified $MTD_{151}-EGF$ was evaluated using cell proliferation assay, cytotoxicity test and skin penetration test by franz diffusion cell assay and artificial skin. Cell proliferation activity of $MTD_{151}-EGF$ purified to high purity of 99% or above was equivalent to the EGF or better, and cytotoxicity was not observed. In addition, the $MTD_{151}-EGF$ showed an excellent penetration efficiency compared to the EGF in the skin penetration test with EGF and $MTD_{151}-EGF$ labeled by FITC in an artificial skin penetration model. Based on the quantitative analysis of the penetrating substance using franz diffusion cell assay, the amount of penetration was about 16 times more than that of EGF. These results can be regarded as an effective alternative to improve the existing physical transdermal penetration method related to the use of various active ingredients for cosmetics.