• Journal of Marine Life Science
• /
• v.6 no.2
• /
• pp.58-65
• /
• 2021

As aging progresses, reactive oxygen species (ROS) reduces skin moisturization and collapses skin barrier function. In this study, we evaluated the efficacy of skin moisturizing and skin barrier function enhancement by extracts from halophytes using HaCaT cells. Spartina anglica (S. anglica; SAE) and Calystegia soldanella (C. soldanella; CSE), a kind of halophytes, were collected from Dongmak beach in Incheon, and extracted with 70% ethanol. At the first, we evaluated the cytotoxicity of extracts in HaCaT cell using WST-8 Kit. As a result, the other experiment was conducted by setting the concentration at which the cell viability was 90% or more. SAE and CSE showed high radical scavenging activity through ABTS assay. Expression levels of genes related to skin moisturizing and skin barrier functions, were analyzed by real-time qPCR. As a result, it showed that the expression of aquaporin 3, hyaluronan synthase 2, and transglutaminase 1 was increased by SAE treatment but not changed by CSE. Activation of extracellular signal regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 mitogen activated protein kinase was induced by SAE. These results suggest that SAE can be used as functional materials for cosmetics for skin moisturizing and barrier function enhancement.

• Journal of the Korean Applied Science and Technology
• /
• v.40 no.5
• /
• pp.936-944
• /
• 2023

The purpose of this study is to verify the effect of essence containing Coptis Japonica extract on skin barrier strengthening and moisturizing. The essence containing the Coptis Japonica extract was used by 22 subjects for four weeks, and the amount of percutaneous water loss (g/m²h) and water (A.U.) in the designated upper limit were measured. As a result of measuring percutaneous water loss using a vapometer, it was found to be 11.08±2.55 after 4 weeks of use from 14.82±4.01 before use, and the significance probability decreased significantly by 25.27% to p<0.001. The amount of skin moisture measured by the Corneometer was found to be 46.73±7.45 after use from 31.73±8.09 before use, and the significance probability increased by 47.26% to p<0.001, and the subject's subjective efficacy evaluation through a questionnaire showed that all subjects were 100% satisfied without skin adverse reactions. As a result of this, it has been confirmed that Coptis Japonica essence is effective in strengthening skin barriers and moisturizing, which is expected to be a cornerstone for the development of cosmetics using various activities of Coptis Japonica extract in the future.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2021.04a
• /
• pp.4-4
• /
• 2021

전 세계는 covid 19의 pandemic 상황이 장기화되면서 화장품 분야의 trend 변화가 일어나고 있다. covid 19의 예방으로 마스크는 생활필수품이 되었고, 행정적으로도 사무공간에서도 마스크를 착용하지 않으면 과태료를 부과하는 상황에 이르렀다. 이로 인하여 장시간 마스크 착용은 필수 불가결한 상황이며, 이로 인하여 얼굴 피부의 장벽기능(barrier function)의 와해로 인하여 피부가 예민해지고 크고 작은 피부의 trouble이 많이 발생하고 있다. 이에 따라 소비자들은 화장품의 선택에 있어 피부 진정과 피부에 자극완화(anti-irritation) 및 안전성(safety)에 대한 것으로 점차적으로 변화하고 있다. Covid 19 이전의 화장품 소재 및 제품 개발의 트렌드는 국내의 기능성화장품 범주에 있는 미백 관련, 주름관련, 자외선 차단관련, 염모관련, 육모 탈모 관련, 여드름관련, 피부장벽관련, 튼살 관련 기능성 소재 및 제품 개발과 화장품 용도외의 고기능성 부여를 위한 코스메슈티컬(cosmeceutical)용 제품의 개발이 중요한 시기였다. 코스메슈티컬(cosmeceutical)은 화장품(cosmetic)과 의약품(pharmaceutical)의 합성어이다. 그러나 최근 Covid 19의 상황에 따라 화장품 업계는 포스트 코로나의 트렌드로 피부 장벽기능 강화, 피부노화 억제, 식물성, 자극완화, 비건, 안전성 확보에 대한 다양한 천연 소재의 연구와 제품을 개발 출시하고 있다. 따라서, 2020년 Covid 19의 상황에 따른 화장품 분야의 천연 신소재 및 화장품 개발 방향에 대하여 자원 식물의 활용성 및 개발 동향에 대하여 논의 하고자한다.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.46 no.4
• /
• pp.361-369
• /
• 2020

Atopic dermatitis (AD) is a common and multifactorial inflammatory skin disease that is characterized by skin barrier dysfunction, inflammation, and chronic pruritus. AD has a complex etiology that includes genetic, immunological, and environmental factors that cause skin barrier abnormalities and immune dysfunctions. Sophora flavescens (SF) has been used in traditional Chinese medicine, but little research has been conducted on its anti-AD efficacy. In this study, we evaluated the effect of SF extract (SFE) on improving skin barrier function and immune abnormalities, which are the main symptoms of AD. SFE has the capacity to enhance the formation of cornified envelope (CE) that plays an important role in the skin barrier function. In addition, it was confirmed that SFE increased the expression of hyaluronic acid related to skin moisture. The effect of SFE against Staphylococcus aureus (S. aureus), which increases specifically in AD lesions, confirmed that SFE inhibited the production of pro-inflammatory cytokines induced by S. aureus. Furthermore, SFE was shown to inhibit the expression of pro-inflammatory cytokines induced by substance P (SP), the cause of skin neurogenic inflammation. These results demonstrate that SFE could be one of potential candidate agent for the treatment of AD by improving the skin barrier function and immune responses.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.49 no.4
• /
• pp.323-330
• /
• 2023

The skin's barrier structure is formed through the differentiation process of epidermal keratinocytes. It consists of corneocytes that are composed of keratin proteins and lipids that fill the spaces between them. During this process, the lipids such as phospholipid that made up the membrane of the basal layer cells of the epidermis are decomposed and replaced with newly synthesized components like ceramide. In this study, the effect of ginsenoside Rg3 components on the packing of the intercellular lipid structure of the skin barrier and the barrier function was confirmed. To confirm this, Rg3 components were treated during the differentiation process of 3D epidermal cells. The FT-IR and TEWL analysis on 3D epidermis showed an enhancement in the orthorhombic lipid packing and an improvement in barrier function. Additionally, in HaCaT cells, an increase in the expression of EVOL1 and EVOL4, which synthesize long-chain lipids, was detected, along with a decrease in CERS6, which synthesizes short-chain ceramide, and an increase in ACER6, which decomposes ceramide using phytosphingosine. This suggests the possibility that Rg3 affects lipid synthesis during the epidermal differentiation process, resulting in changes in barrier function.

• Korean Journal of Food Science and Technology
• /
• v.40 no.6
• /
• pp.686-690
• /
• 2008

Recent research has shown that curcumin has beneficial effects in a variety of skin diseases, including scleroderma, psoriasis, and skin cancer. In this study, we assessed the effects of curcumin on epidermal permeability barrier function in vivo and in vitro. In order to evaluate the effects of curcumin on epidermal permeability barrier function in vivo, hairless rats were exposed to UVB irradiation, and curcumin was administered orally at a dosage of 150 mg/kg per day for 8 weeks. Transepidermal water loss (TEWL) and epidermal thickness were measured at the end of the experiment. The expression of filaggrin, a marker of keratinocyte differentiation, and serine palmitoyltransferase (SPT), a marker of the formation of the stratum corneum lipid barrier, in human HaCat keratinocytes were analyzed. The in vivo results showed that an 8 week administration of curcumin markedly prevented the UVB-induced increase in TEWL. The UV-induced increase in epidermal thickness was also reduced significantly by curcumin treatment. The in vitro results demonstrated the concentration-dependent effects of curcumin on the expression of both filaggrin and SPT in HaCat cells, reflecting the notion that curcumin can induce epidermal keratinocyte differentiation and can improve the recovery of skin barrier functions. These results show that curcumin is a promising candidate for the improvement of epidermal permeability barrier function.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.45 no.4
• /
• pp.389-397
• /
• 2019

Lysosomes are cellular organelles involved in energy metabolism and intracellular digestion in eukaryotic cells, including protease, nuclease, glycosidase, lipase, and phosphatase. Our previous studies have confirmed that egg white lysosomes had melanin decolorization and reduction activity. However, there have been few studies on skin barrier and skin regeneration as well as inhibition of melanin production by egg white lysosomes on B16F10 melanocyte cell line. In this study, we attempted to identify the effect of lysosome-related organelle extract (LOE) extracted from egg white on the melanin content change and skin barrier enhancement in cells. First, cytotoxicity evaluation was performed on B16F10 melanocyte cell line to confirm the whitening efficacy of LOE. Cytotoxicity by LOE was not observed at 20 mg/mL concentration, but cytotoxicity was observed at 40 mg/mL, and the maximum concentration value was set to 20 mg/mL in all subsequent experiments. LOE samples of 5, 10, 20 mg/mL inhibited melanin production by 61.5 ± 4.0%, 61.4 ± 7.3%, 58.3 ± 8.3%, respectivly, compared to α-MSH, a negative control in melanin contents assay. MITF mRNA expression was reduced by about 39.7 ± 3.2% compared to the α-MSH treatment group. TEER assay using HaCaT showed that LOE increased TEER resistance in a dose-dependent manner, indicating that LOE is involved in strengthening the skin barrier. LOE also increased the TEER resistance under TNF-α treatment. Skin barrier was normally restored by LOE even under the condition of inflammation. LOE had a positive effect on cell division and cell migration promotion, confirmed by the observing the effect of promoting cell migration by LOE through cell migration assay. Taken together, we expect that LOE can be developed as a cosmetic material to enhance has effects on skin regeneration and skin barrier strengthening as well as whitening function if enzyme stabilization and formulation technology are combined.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.40 no.1
• /
• pp.21-28
• /
• 2014

In this study, Eucommia ulmoides Oliver extracts was studied in order to see any effects on the ${\beta}$-hexosaminidase release suppression of RBL-2H3 cells and on the expression of filaggrin, transglutaminase-1 (TGase-1) and cornified cell envelope (CE) related to the recovery of HaCaT keratinocyte skin barrier. Results showed that Eucommia ulmoides Oliver extracts reduced ${\beta}$-hexosaminidase release in RBL-2H3 cells and increased the effects of Eucommia ulmoides Oliver extract on the expression of filaggrin, transglutaminase-1 (TGase-1) and cornified cell envelope (CE) in HaCaT keratinocytes. Taken together, these results suggested that Eucommia ulmoides Oliver extract may be applicable for keratinocyte differentiation.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.47 no.1
• /
• pp.65-73
• /
• 2021

It is very important to control the inflammation of the skin because it can develop into diseases such as atopy as well as scarring and aging. In this work, we recently identified the in vitro synthesis of specialized pro-resolving mediators (SPMs) known to control inflammation in the human body and the applicability of cosmetics. Using recombinant protein of lipoxygenase from Glycine max, we succeeded to prepare mixtures of mono- or di-hydroxy DHA named as S-SPMs and used them for in vitro efficacy test. To investigate anti-inflammatory effect of S-SPMs, mRNA level of TNF-α and IL-6 were analyzed. Under UVB exposed condition, expression of both were decreased by S-SPMs treatment. And we observed reduced production of nitric oxide (NO) by S-SPMs application under the condition with diesel particulate matter (DPM). At the same experimental condition, malondialdehyde (MDA) production was decreased by S-SPMs, indicating the inhibitory effect of S-SPMs in lipid peroxidation. In addition, S-SPMs treatment resulted in reduction of matrix metalloproteinases-1 (MMP-1) expression and elevation of procollagen type I synthesis. Together with this, mRNA level of filaggrin and loricrin were increased by S-SPMs, indicating enhancement of skin barrier function. These results demonstrate that S-SPMs is a good candidate to develop as a cosmetic ingredient for anti-inflammation, anti-wrinkle, and improvement of skin barrier function.

• Journal of the Korean Applied Science and Technology
• /
• v.35 no.4
• /
• pp.1243-1249
• /
• 2018

Ceramides are commonly studied and developed in the cosmetics industry as ingredients that help a moisturized skin and strengthen a skin barrier. In this study, we determined how much great effects the moisturing cream containing a ceramide, obtained from evening primrose oil instead of using a common synthetic ceramide, made on the changes in moisturizing and transdermal water loss of skin. The result showed that the cream with a ceramide showed much better results than the cream without a ceramide at the skin moisture resistance and transdermal water loss of skin.