Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Effects of Eucommia ulmoides Oliver Extract on Inhibition of β-hexosaminidase and Keratinocyte Differentiation

β-hexosaminidase 분비 억제 및 각질형성세포 분화에 대한 두충(Eucommia ulmoides Oliver) 추출물의 효과

  • Received : 2013.08.08
  • Accepted : 2013.09.29
  • Published : 2014.03.31
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Abstract

In this study, Eucommia ulmoides Oliver extracts was studied in order to see any effects on the ${\beta}$-hexosaminidase release suppression of RBL-2H3 cells and on the expression of filaggrin, transglutaminase-1 (TGase-1) and cornified cell envelope (CE) related to the recovery of HaCaT keratinocyte skin barrier. Results showed that Eucommia ulmoides Oliver extracts reduced ${\beta}$-hexosaminidase release in RBL-2H3 cells and increased the effects of Eucommia ulmoides Oliver extract on the expression of filaggrin, transglutaminase-1 (TGase-1) and cornified cell envelope (CE) in HaCaT keratinocytes. Taken together, these results suggested that Eucommia ulmoides Oliver extract may be applicable for keratinocyte differentiation.

본 연구에서는 두충 추출물이 RBL-2H3 세포의 ${\beta}$-hexosaminidase의 분비 억제와 HaCaT keratinocytes 피부장벽의 회복과 관련한 filaggrin, transglutaminase-1 (TGase-1), cornified cell envelope(CE)의 발현에 미치는 영향에 관하여 연구하였다. ${\beta}$-hexosaminidase 방출 억제 능은 13% 효능을 확인하였고, 피부장벽기능의 회복과 관련된 인자들은 발현과 활성의 정도가 매우 우수한 것을 확인하였다. 각질형성세포의 분화를 판단할 수 있는 CE 측정에서는 두충추출물이 양성대조군보다 더 좋은 효능을 나타내기도 하였다. 따라서 두충 추출물은 ${\beta}$-hexosaminidase 분비 억제에 효과가 있으며, 손상된 피부장벽강화에 영향을 미치는 각질형성세포의 분화 촉진에 효과가 있음을 확인하였다.

Keywords

References

  1. C. H. Lee, Sensitive skin, The korean society for skin barrier research, 11(2), 55 (2009).
  2. P. M. Elias, L. C. Wood, and K. R. Feingold, Epidermal pathogenesis of inflammatory dermatoses, Am. J. Contact Dermatol., 10(3), 119 (1999).
  3. J. P. Allam, T. Bieber, and N. Novak, Recent high-lights in the athophysiology of atopic eczema, Int. Arch. Allergy. Immunol., 136(2), 191 (2005). https://doi.org/10.1159/000083893
  4. H. Zhang, Immunopharmacological effect of Cordyceps sinensis, Zhong Xi Yi Jie He Za Zhi, 10(9), 570 (1990).
  5. H. J. Cho, J. Y. Lee, and D. G. Kim, In vitro and in vivo Anti-inflammatory and Anti-allergic responses caused by water extract of Mori Cortex, J. Korean Oriental Pediatrics, 19(2), 175 (2005).
  6. P. D. Moon, J. J. Na, H. J. Jeong, S. H. Hong, H. R. Kim, J. O. Choi, S. H. Lee, J. Y. Shin, and H. M. Kim. Inhibitory effect of Gamibojungikqitang extract on mast cell-mediated allergicreaction in murine Model, J. Pharm. Sci., 8(1), 94 (2005).
  7. M. Ennis, F. L. Pearce, and P. M. Weston, Some studies on the release of histamine from mast cells stimulated with polylysine, Br. J. Pharmacol., 70(2), 329 (1980). https://doi.org/10.1111/j.1476-5381.1980.tb07940.x
  8. G. C. Yen and C. L. Hsieh, Reactive oxygen species scavenging activity of Du-zhong (Eucommia ulmoides oliv.) and its active compounds, J. Agric. Food Chem., 48(8), 3431 (2000). https://doi.org/10.1021/jf000150t
  9. C. L. Hsieh and G. C. Yen, Antioxidant actions of du-zhong (Eucommia ulmoides oliv.) toward oxidative damage in biomolecules, Life Sci., 66(15), 1387 (2000). https://doi.org/10.1016/S0024-3205(00)00450-1
  10. Y. F. Sasaki, A. Chiba, M. Murakami, K. Sekihashi, M. Tanaka, M. Takahoko, S. Moribayashi, C. Kudou, Y. Hara, Y. Nakazawa, T. Nakamura, and S. Onizuka, Antimutagenicity of Tochu tea (an aqueous extract of Eucommia ulmoides leaves): 2. Suppressing effect of Tochu tea on the urine mutagenicity after ingestion of raw fish and cooked beef, Mutat. Res., 371(3-4), 203 (1996). https://doi.org/10.1016/S0165-1218(96)90108-1
  11. T. Nakamura, Y. Nakazawa, S. Onizuka, A. Chiba, K. Sekihashi, A. Miura, N. Tasugahira, and Y. F. Sasaki, Antimutagenicity of Tochu tea (an aqueous extract of Eucommia ulmoides leaves) 1. The clastogen- suppressing effects of Tochu tea in CHO cell and mice, Mutat. Res., 388(1), 7 (1997). https://doi.org/10.1016/S1383-5718(96)00096-4
  12. J. Watanabe, J. Kawabata, H. Kurihara, and R. Niki, Isolation and identification of alpha-glucosidase inhibitors from Tochu tea (Eucommia ulmoides), Biosci. Biotechnol. Biochem., 61(1), 177 (1997). https://doi.org/10.1271/bbb.61.177
  13. Y. Li, T. Sato, K. Metonri, K. Koike, Q. M. Che, and S. Takahashi, The promoting effects of geniposidic acid and aucubin in Eucommia ulmoides oliver leaves on collagen synthesis, Biol. Pharm. Bull., 21(12), 1306 (1998). https://doi.org/10.1248/bpb.21.1306
  14. S. P. Choi, M. Y. Kang, and S. H. Nam, Inhibitory activity of pigmented rice bran extract to the allergic inflammation in basophilic cell line and peritoneal mast cells, J. Korean Soc. Appl. Biol. Chem., 48(4), 315 (2005).
  15. O. B. Choi, Anti-allergic Effects of Petasites japonicum, Korean J. Food & Nutr., 15(4), 382 (2002).
  16. P. Yamada, M. Zarrouk, K. Kawasaki, and H. Isoda, Inhibitory effect of various Tunisian olive oils on chemical mediator release and cytokine production by basophilic cells, J. Ethnopharmacol., 116(2), 279 (2008). https://doi.org/10.1016/j.jep.2007.11.028
  17. B. A. Dale, Purification and characterization of a basic protein from the stratum cormeum of mammalian epidermis, Biochem. Biophy. Acta., 491(1), 193 (1977).
  18. R. L. Meek, J. D. Lonscale-Eccles, and B. A. Dale, Epidermal filaggrin is synthesized on a large messenger ribonucleic acid as a high-molecular-weight preecursor, Biochemistry, 22(21), 4867 (1983). https://doi.org/10.1021/bi00290a001
  19. J. M. Yang, Mutations of transglutaminase 1 gene in lamellar ichthyisis, The JSBR., 2(1), 22 (2000).
  20. A. C. Steven and P. M. Steinert, The protein composition of cornified cell envelopes, J. Cell Sci., 107(2), 693 (1994).
  21. P. M. Steinert and L. N. Marekov, The proteins elafin, rilaggirn. keratin, intermediate rilaments, loricrin, and small prolinerich proteins 1 and 2 are isodipeptide cross-linked components of the human epikermal cornified cell envelope, J. Biol. Chem., 270(30), 17702 (1995). https://doi.org/10.1074/jbc.270.30.17702
  22. R. Schmidt, U. Reichert, and M. Bouclier, Plasma membrane transglutaminase and cornified envelope competence in vultured human keratinocytes, REBS Lett., 186(2), 201 (1985).

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