• Korean Journal of Ichthyology
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• v.20 no.4
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• pp.272-278
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• 2008

Optimum concentration of alizarine complexone (ALC) for staining of otoliths for Pacific cod was determined by examination of stained quality, growth and survival rates. Small juveniles ($34.1{\pm}1.8mm$ TL, $mean{\pm}SD$) were immersed in four different concentrations (20, 40, 60, 80 mg/L) and large ones ($73.8{\pm}1.2mm$ TL, $mean{\pm}SD$) in five different concentrations (1, 5, 10, 20, 40 mg/L) of ALC for 24 hrs. The fish were then reared and observed for 14 days. The stained quality of otoliths was evaluated by dividing them into four grades by the presence of fluorescent stains on the otoliths under G-excited UV light. The small juveniles stained with the different ALC concentrations had 100% staining success, and their growth rate and body weight did not differ significantly between treatments. Optimal ALC concentration for the smaller juveniles was 20 mg/L in the present experiment. On the other hand, distinct fluorescent rings were confirmed on otoliths of larger juveniles in the 10 mg/L treatment with high survival rate. These results suggest that ALC staining at concentrations of 10 mg/L with larger juveniles will be helpful for release-recapture experiments for stock enhancement of Pacific cod.

• Journal of fish pathology
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• v.27 no.2
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• pp.127-131
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• 2014

The suitable tag was investigated based on the cumulative mortality, blood chemistry and histopathology of olive flounder, Paralichthys olivaceus. The dissection of opercle, 50% dissection of pelvic fin and 100% dissection of pelvic fin was used in the experiment. Cumulative mortality of dissection of opercle group was high. The dissection of opercle and 100% pelvic fin dissection groups was showing histological changes after 13days of tagging: purulent inflammation in the liver, fibrous inflammation in the body kidney, hyperplastic ellipsoide capillary in the spleen. As the results, 50% pelvic fin cutting group is the most effective out of them.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.3
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• pp.282-288
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• 2002

This study was carried out to investigate the optimum tagging method of the abalone juvenile, Haliotis discus hannai in indoor culture system from May 2000 to January2001. Tagging methods were shell drilling, copper-wire tagging at the respiratory pore and nut gluing on the shell. The attachment rates of the shell showed high in the 2- and 3 cm bolt-nut tagged groups, about over $89.5\%$, whereas shell drilling groups on the shell were about $18.5\%$. The internal coating rates tagged with bolt-nut were over $96.6\%$, while those tagged with copper wire were less than $17.1\%$. Growths in the all marked and tagged experimental groups comparing with control groups were not significantly different (p>0,05). Survivals in all tagging groups except shell drilling and nut gluing groups in the 5 cm abalone were over $95\%, Accordingly, all juvenile groups were not affected by the tagging methods in terms of the growth and survival on the abalones. Based on these results, the micro bolt-nut tagging was the most effective method in abalone.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2003.05a
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• pp.365-366
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• 2003

주꾸미는 서해 연안 어족의 자원감소와 함께 어장 환경 악화에 의해 매년 생산량이 감소되고 있으며, 금후에는 양적 성장이 어려울 뿐만 아니라 어업인의 소득 감소가 예상되고 있다. 따라서 주꾸미의 생산량을 지속적이고 안정적으로 유지하기 위해서는 지금까지의 어선ㆍ어업에서 탈피하여 주꾸미의 인공 종묘생산 및 자원조성을 위한 종묘의 대량 방류가 필요하다. 주꾸미 종묘생산시 시료의 무게 측정, 표지작업, 인공채란, 수송 그리고 질병치료 등을 위해 주꾸미 유생을 그물망으로 포획하는 것은 유생에 물리적 손상과 스트레스를 주어 폐사의 1차 원인이 되기도 한다. (중략)

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.41 no.1
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• pp.1-8
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• 2005

In order to find out the environmental factors influencing movement of common grey mullet, Mugil cephalus in funnel net fishing ground of the Dolsan-do, Yeosu southern sea area, the oceanographic factor such as the water temperature, isobath and tidal current were observed respectively, the water temperature was compared with the amount of common grey mullet caught by funnel net. Also, to investigate the movement direction of common grey mullet in same sea area, 160 common grey mullets of body length 22 to 51cm caught at funnel nets of the Dolsan-do southern sea area were marked and then released at 5 positions in 5 times. The results obtained are summarized as follows : 1. The water temperature at the funnel net fishing ground of Dolsan-do in 2002 was ranged from 6.9 to 27.4$^{\circ}C$. The water temperature was displayed a maximum value in August to increase from March and a minimum value in February of the ensuing year to decrease from September. The catches of gray mullet caught by funnel net were generally abundant from March to September, but decreased sharply from October. The optimum range of water temperature for the funnel nets fishing was situated between 15.0 to 25.0$^{\circ}C$. 2. The isobath from 6m to 13m in coast sea set up funnel nets were densely distributed and the depth more than 14m of isobath were widely spreaded to the open sea at Dolsan-do southern sea area. 3. The tidal current of the coast sea set up funnel nets flowed southward and northward along the coast ato ebb and flood tide respectively. The direction of tidal current to the open sea was southeast at ebb tide with the mean speed 43cm/sec, but northwest at flood with the mean speed 25cm/sec. 4. The recapture rate through the experiment duration showed 9.4%. The recapture rate in Gyedong area was very high value with 33.3% as compared with others. The movement of common grey mullet in Dolsan-do southern sea area trended toward a inner bay and north bound mainly.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.45 no.5
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• pp.499-506
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• 2012

We estimated the population size and fishing mortality of Pacific cod Gadus macrocephalus during the spawning season in waters off Woipo, Geoje Island, Korea, using a mark-recapture method. We marked and released 51 cod>50 cm in total length; six were recaptured by local fishermen during the period from December 15 to 31, 2009. The estimated population size was ca. 180,000 and the fishing mortality of the exploitable cod was 26%. Although we could assume a closed population due to the short survey period, we evaluated the uncertainty in the estimates by applying bootstrap resampling because the sample size was small. The estimated 95% confidence interval was 94,000-568,000 for the population size and 8-49% for fishing mortality. Our study demonstrated that the application of mark-recapture methods and bootstrap resampling can be useful in stock assessment for fisheries management in Korea, but requires a larger sample size, spatially extensive coverage, and sophisticated mark-recapture models based on a refined sampling design for reliable stock assessment and biological reference points in sustainable cod management.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.17 no.4
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• pp.292-302
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• 2012

This paper reviews comparison analysis of current and latest application for stock identification methods of Todarodes pacificus, and the pros and cons of each method and consideration of how to compensate for each other. Todarodes pacificus which migrates wide areas in western North Pacific is important fishery resource ecologically and commercially. Todarodes pacificus is also considered as 'biological indicator' of ocean environmental changes. And changes in its short and long term catch and distribution area occur along with environmental changes. For example, while the catch of pollack, a cold water fish, has dramatically decreased until today after the climate regime shift in 1987/1988, the catch of Todarodes pacificus has been dramatically increased. Regarding the decrease in pollack catch, overfishing and climate changes were considered as the main causes, but there has been no definite reason until today. One of the reasons why there is no definite answer is related with no proper analysis about ecological and environmental aspects based on stock identification. Subpopulation is a group sharing the same gene pool through sexual reproduction process within limited boundaries having similar ecological characteristics. Each individual with same stock might be affected by different environment in temporal and spatial during the process of spawning, recruitment and then reproduction. Thereby, accurate stock analysis about the species can play an efficient alternative to comply with effective resource management and rapid changes. Four main stock analysis were applied to Todarodes pacificus: Morphologic Method, Ecological Method, Tagging Method, Genetic Method. Ecological method is studies for analysis of differences in spawning grounds by analysing the individual ecological change, distribution, migration status, parasitic state of parasite, kinds of parasite and parasite infection rate etc. Currently the method has been studying lively can identify the group in the similar environment. However It is difficult to know to identify the same genetic group in each other. Tagging Method is direct method. It can analyse cohort's migration, distribution and location of spawning, but it is very difficult to recapture tagged squids and hard to tag juveniles. Genetic method, which is for useful fishery resource stock analysis has provided the basic information regarding resource management study. Genetic method for stock analysis is determined according to markers' sensitivity and need to select high multiform of genetic markers. For stock identification, isozyme multiform has been used for genetic markers. Recently there is increase in use of makers with high range variability among DNA sequencing like mitochondria, microsatellite. Even the current morphologic method, tagging method and ecological method played important rolls through finding Todarodes pacificus' life cycle, migration route and changes in spawning grounds, it is still difficult to analyze the stock of Todarodes pacificus as those are distributed in difference seas. Lately, by taking advantages of each stock analysis method, more complicated method is being applied. If based on such analysis and genetic method for improvement are played, there will be much advance in management system for the resource fluctuation of Todarodes pacificus.

• Korean Journal of Environmental Agriculture
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• v.30 no.1
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• pp.37-42
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• 2011

BACKGROUND: The purpose of this study was to evaluate ecological function of small-scale pond and movement characteristics of mudfish (Misgurnus mizolepis) during midsummer drainage period. METHODS AND RESULTS: In situ experiments were performed in the paddy field with mudfish under the condition of midsummer drainage from 13 July to 29 July 2010. The mudfish used in this experiment is approximately 1,000 individuals with a cut tail. Mudfishs were released in the rice field before midsummer drainage and caught again in the small-scale pond and the paddy field after midsummer drainage. Results showed that the abundance of mudfish was higher in drainage canal than small-scale pond at the early stage of midsummer drainage, because flow was formed toward the drainage canal. In that time, 3% of the total marked mudfish were captured at outlet of drainage canal. As the paddy was drying, 5% of total marked mudfish moved to the small-scale pond during midsummer drainage period. Contrary to the general hypothesis, the marked mudfish was not found in holes in paddy field.of total caught in the small-scale pond ingested mainly animal prey, and it's frequency of empcy stomach was 10%.oOn the other hand, all m total collected in the paddy field showed empcy stomach. It was apparent from the experiment that m total are eeldng normally in the small-scale pond, while m total are not eat properly in paddy field. CONCLUSION(s): As the paddy was drying, mudfish moved to the small-scale pond during midsummer drainage period but mudfish was not found in holes in paddy field. It can be concluded that small-scale provides a shelter and prey to mudfish in the midsummer drainage period.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.51 no.2
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• pp.227-234
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• 2015

To obtain geographical range and growth-kinetics parameters of mottled sake (Beringraja pulchra) populations in the Yellow Sea, three mark-recapture experiments were carried out. Overall, 991 tagged individuals were released, and 4.1% of them were recaptured with the mean release period of 339 d (range, 8-1,420 d) and the mean growth rate of $1.4cm\;mon^{-1}$ (female, $1.5cm\;mon^{-1}$; male $1.3cm\;mon^{-1}$). In the first experiment, 667 individuals were released at Heuksan Island from April to June, 2007-2009, and 30 individuals were recaptured mainly at the north and the north-east coasts of the island, indicating absence of migration to the south of the island. In the second experiment, 323 individuals were released at several fishing grounds scattered in the Yellow Sea in 2010-2013, and 11 individuals were recaptured at points deviated to all directions from the releasing points. As the last, one individual was released with pop-up satellite archival tag at a costal point ($34^{\circ}37.2$'N, $124^{\circ}59.3$'E) off Hong Island on May 21, 2010. The tagged individual migrated to a north-east location ($35^{\circ}50.4$'N, $126^{\circ}03.6$'E) of Eocheong Island by Aug. 25, 2010. The data archived for the three months in the tag indicated that the migration path had depths of 48-80 m and temperature of $12.6-14.4^{\circ}C$. The results indicated that mottled sake populations had a localized habitat ranges at the north of Heuksan Island and the west of Hong Island while growing at the rate of $1.4cm\;mon^{-1}$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.6
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• pp.709-716
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• 2011

To investigate the distribution and migrations of sea turtles occurring in Korean waters, an adult female green sea turtle Chelonia mydas was tagged with a satellite transmitter and released at Busan in October 2009. The confirmation was made for the first time that the released green sea turtle migrated back to Korea after visiting Jeju Island and Japan for approximately 9 months. The green sea turtle spent the winter in Jeju and Japan because of low water temperatures in the East Sea and South Sea during winter months, which were not suitable for turtle survival. Compared with an immature green sea turtle that was released at Jeju Island, the adult green showed a markedly different migration pattern, suggesting that juvenile and adult greens move differently because of their peculiar life cycle and their stage of maturity, in addition to responses to temperature changes. Additional satellite tracking with increased representation of species, sexes and sizes of turtles, and regions is required to gather information on the distribution and movements of sea turtles in Korean waters.