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Effect of Red LED, Blue LED and UVa Light Sources on Coloration of Paprika Fruits (적색 및 청색 LED광과 UVa광 처리가 착색단고추 과실의 착색에 미치는 영향)

  • Choi, In-Lee;Won, Jae-Hee;Jung, Hyun-Jin;Kang, Ho-Min
    • Journal of Bio-Environment Control
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    • v.18 no.4
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    • pp.431-435
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    • 2009
  • This study was conducted to compare the effect of various LED and UVa light to enhanced coloration of paprika fruits cultivars; 'Special', 'Helsinki'. Immatured paprika fruits were irradiated with red LED, blue LED and UVa lights which were high intensity (red: $50{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, blue: $70{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, UVa: $3{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and low intensity (red: $20{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, blue: $40{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, UVa: $0.25{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$). The coloration of 'Special' cultivar was increased the fastest in high intensity UVa light for 2 days after treatment. There were no significantly difference among treatments except the low intensity UVa light. The coloration of 'Helsinki' was increased the fastest in blue LED light, and followed by red LED light and UVa light for 2 days after treatment. The $a^*/b^*$ value of 'Special' and $b^*$ value of 'Helsinki' that indicated the paprika fruit coloration were generally higher high intensity LED and UVa lights than low intensity LED and UVa lights. We couldn't find out any significantly different coloration effect among all treatments in final days of coloration. and rather than UVa treatment fruit shrunk the surface of paprika fruits.

Difference of Absorption and Anatomical Responses to Protoporphyrinogen Oxidase-Inhibiting Herbicides in Wheat and Barley (Protoporphyrinogen Oxidase 저해형 제초제에 대한 밀과 보리의 흡수 및 해부하적 차이)

  • 구자옥;국용인
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.1
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    • pp.68-78
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    • 1997
  • Laboratory and greenhouse studies were conducted to determine differential sensitivities on absorption of $^{14}$ C-oxyfluorfen and the anatomical responses in wheat and barley to protoporphyrinogen oxidase-inhibiting herbicides [oxyfluorfen (2-chloro-1- (3-ethoxy -nitrophen-oxy)-4-(trifluoromethyl) benzene, acifluorfen(5-[2-chloro-4-(trifluoromethyl) phenoxy]-2-nitro-benzoic acid), bifenox(methyl-5-(2, 4-dichlorophenoxy)-2-nitrobenzoate) and oxadiazon(5-tert-butyl-3-(2, 4-dichloro-5-isopropoxyphenyl)-1, 3, 4-oxadiazol-2-one)]. I$_{50}$ value of the tolerant wheat cultivars to oxyfluorfen was about 10$^{-4}$ , whereas that of the susceptible barley cultivars was about 10$^{-6}$ M, showing significant difference between the two groups. When foliage were applied with acifluorfen, bifenox or oxadiazon, the oxyfluorfen-tolerant wheat showed less decreased in shoot fresh weight and chlorophyll content than the susceptible barley. Also, when soil-applied with these herbicides test plants showed similar tendency in foliar application. Electrolyte leakage from the tissue treated with these compounds was the more influenced in the barley than the wheat. Malondialdehyde(MDA) production as index of lipid peroxidation was greater in the barley than the wheat by treatment of these compounds. Therefore, the differential sensitivities of wheat and barley to protoporphyrinogen oxidaseinhibiting herbicides was showed by our greenhouse and in vitro experiment. The absorption rates of $^{14}$ C-oxyfluorfen were higher in the barley than the wheat. And this tendency was showed appararitly difference by increase of treatment durations. After the oxfluorfen and oxadiazon treatment, the tolerant wheat did not show the structural damage in leaf surface, but the susceptible barley was damaged in the leaf waxy layer. However, the acifluorfen and bifenox treatment showed no difference between wheat and barley. The anatomical changes by these compounds treatment were not observed in the tolerant wheat but epidermal cell and mesophyll cell were highly broken in the susceptible barley.

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Study on Plrene Removal Characteristic From An Artificially Contaminated EPA Synthetic Soil Matrix With Varying Heat Treatment Conditions (Pyrene으로 오염된 EPA토양의 열적처리조건에 따른 오염물질 제거 특성 연구)

  • 김영규;양고수
    • Journal of Korea Soil Environment Society
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    • v.5 no.2
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    • pp.55-66
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    • 2000
  • A U.S EPA Synthetic soil matrix was used for reference neat soil and pyrene contaminated soil. For the contaminated soil, 4.79 wt.% pyrene was dissolved completely into the djchlorornethane, and the soil was evenly soaked with the pyrene solution. The contaminated soil samples(50$\pm$0.5mg) were heated in a modified electrical screen heater reactor which consisted of a thin stainless foil (3.5cm$\times$13cm$\times$0.00254cm, 302 stainless steel shim), two electrodes, and a 20cm dia. $\times$30cm tall cylindrical Pyrex chamber sealed at both ends by aluminum flanges. The heating rate and time conditions were selected as $455^{\circ}C$ @ $1137^{\circ}C$ /s, $760^{\circ}C$ @ $950^{\circ}C$ /s and $977^{\circ}C$ @ $977^{\circ}C$/s. Tar samples after heating the soils were collected on the aluminum foil funnel and a glass filter paper (25mm dia. filter paper) The tar sample and remnant soil on the reactor were extracted with dichloromethane covering the filters, foils and soil by sonicating each in the waterbath for 10 minutes. The extractions were run on a HPLC. At the low peak temperature(about $455^{\circ}C$ @ $1137^{\circ}C$/s) the color of tar was "white", at the middle peak temperature (about 76$0^{\circ}C$ @ 95$0^{\circ}C$/s) the color of tar was "pink brown", at the high peak temperature (about 977$^{\circ}C$ @ 977$^{\circ}C$/s) the color of tar was "dark brown". Cyclopeta(cd)pyrene (CPEP) , which is an interesting species due to mutagenic effect on human cells, was detected in tar samples only above the middle peak temperature. This species was not detected at the low peak temperature. Six isomers of bipyrene were detected. Phenanthrene(C$_{14}$ $H_{10}$) and cyclopenta(def)phenanthrene(C$_{15}$ $H_{10}$) were also detected, but their content was very small relative to the other listed compounds.to the other listed compounds.

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Effects of mechanical stress and interleukin-$1{\beta}$ on collagenase and TIMP-1 expression in human periodontal ligament fibroblasts (기계적 자극과 interleukin-$1{\beta}$가 치주인대 섬유아세포의 collagenase와 TIMP-1의 발현에 미치는 영향)

  • Kim, Myung-Lip;Bae, Chang
    • The korean journal of orthodontics
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    • v.28 no.1 s.66
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    • pp.165-174
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    • 1998
  • The turnover of collagen is controlled by the balance between collagen synthesis and degradation. The production of collagenase (matrix metalloproteinase-1) and its inhibitor, tissue inhibitor of matrix metallopmteinase-1 (TIMP-1) are one of the substances which regulate this balance. The periodontal ligament fibroblast plays an important role in collagen metabolism during orthodontic treatment and is believed to be an origin of the osteoblast in the alveolar bone. The collagenase secreted by the periodontal ligament fibroblast and the osteoblast initiates the bone resorption by removing the osteoid layer in the alveloar bone. The interleukin-$1{\beta}$ is secreted by the macrophage during orthodontic treatment. The present study was undertaken to assess the effect of mechanical stress and interleukin-$1{\beta}$ on the expression of collagenase and TIMP-1 in the periodontal ligament fibroblasts using reverse transcription polymerase chain reaction and immunohistochemical staining. The periodontal ligament fibroblasts were stitched by placing the $Petriperm dish^{\circledR}$ dish on the top of spheroidal convex watch glass ($5\%$ surface increase) and tented with interleukin-$1{\beta}$ (1.0 ng/ml), or treated with both of them. Treatment with mechanical stress and/or interleukin-$1{\beta}$ resulted in increased collagenase mRNA expression. The mechanical stress treated group (1.61, 1.62, 1.37 fold increase), the interleukin-$1{\beta}$, tented group (1.68, 1.60, 3.78 fold increase), the mechanical stress and interleukin-$1{\beta}$ treated group (1.89, 1.72, 5.48 fold increase) induced increases in collagenase mRNA compared with the control group after 2, 4, 8 hours respectively. But TIMP-1 mRNA expressions at experimental groups were decreased after 2, 4 hours and increased after 8 hours. The mechanical stress treated group (0.16, 0.49 fold decrease and 3.77 fold increase), the interleukin-$1{\beta}$ treated group (0.15,0.44 fold decrease and 4.46 fold increase), the mechanical stress and interleukin-$1{\beta}$ tented group (0.15, 0.69 fold decrease and 4.81 fold increase) induced changes in TIMP-1 mRNA compared with the control group after 2, 4, 8 hours, respectively. Immunohistochemical stain showed that increased collagenase and TIMP-1 staining of the mechanical stress tented group, the interleukin-$1{\beta}$ treated group, and the mechanical stress and interleukin-$1{\beta}$ treated group compared with that of the control group after 8 hours. These findings suggest that mechanical stress and interleukin-$1{\beta}$ regulate expression of collagenase and TIMP-1.

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Optimization of Ingredients for the Preparation of Chinese Quince (Chaenomelis sinensis) Jam by Mixture Design (모과잼 제조시 혼합물 실험계획법에 의한 재료 혼합비율의 최적화)

  • Lee, Eun-Young;Jang, Myung-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.7
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    • pp.935-945
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    • 2009
  • This study was performed to find the optimum ratio of ingredients in the Chinese quince jam. The experiment was designed according to the D-optimal design of mixture design, which included 14 experimental points with 4 replicates for three independent variables (Chinese quince paste $45{\sim}60%$, pectin $1.5{\sim}4.5%$, sugar $45.5{\sim}63.5%$). A mathematical analytical tool was employed for the optimization of typical ingredients. The canonical form and trace plot showed the influence of each ingredient in the mixture against final product. By use of F-test, sweetness, pH, L, b, ${\Delta}E$, and firmness were expressed by a linear model, while the spreadmeter value, a, and sensory characteristics (appearance, color, smell, taste, and overall acceptability) were by a quadratic model. The optimum formulations by numerical and graphical method were similar: Chinese quince paste 54.48%, pectin 2.45%, and sugar 53.07%. Optimum ingredient formulation is expected to improve use of Chinese quince and contribute to commercialization of high quality Chinese quince jam.

Optimization of Extraction Conditions for Useful Components from Sweet Potato Leaves (고구마 잎의 유용성분 추출조건 최적화)

  • Li, Meishan;Jang, Gwi Yeong;Lee, Sang Hoon;Kim, Sung Tae;Lee, Ji Hyun;Hwang, Se Gu;Sin, Hyun Man;Kim, Hong Sig;Kang, Tae Su;Jeong, Heon Sang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.11
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    • pp.1749-1756
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    • 2014
  • This study was performed to establish extraction conditions for useful components from sweet potato leaves. Extraction conditions were heating temperature ($40{\sim}60^{\circ}C$), ratio of solvent to sample (10~30 mL/g), and ethanol concentrations (50~90%). The maximum lutein content was $381.88{\mu}g/g$ under the following conditions: 81.23% ethanol concentration, $46.75^{\circ}C$ temperature, and 25.78 mL/g ratio of solvent. The maximum total polyphenol content was 58.44 mg GAE/g under 67.0%, $53.90^{\circ}C$, and 29.10 mL/g, whereas maximum total chlorophyll content was 769.14 mg/100 g under 93.10%, $69.90^{\circ}C$, and 25.98 mL/g, respectively. The highest antioxidant activity of electron-donating ability ($IC_{50}$) was 0.582 mg/mL under 64.56%, $54.63^{\circ}C$, and 19.57 mL/g, whereas total antioxidant activity was 32.54 mg AA eq/g under 64.42%, $44.51^{\circ}C$, and 26.01 mL/g, respectively. Under these conditions, experimental data matched well with the predicted data. Consequently, the present study was carried out to simulate and optimize sweet potato leaves for useful component extraction.

Quality Characteristics of Hard Roll Bread with Concentrated Sweet Pumpkin Powder (농축단호박 분말을 대체한 하드롤 빵의 품질 특성)

  • Lee, Chan-Ho;Chun, Soon-Sil;Kim, Mun-Yong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.7
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    • pp.914-920
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    • 2008
  • In this study, hard roll breads were prepared with 3, 6, 9, 12, and 15% of concentrated sweet pumpkin powder (CSPP). The samples and a control were then compared in terms of quality characteristics, including pH, total titratable acidity, fermentation power of dough expansion, specific volume, baking loss, moisture content, color, textural characteristics, external and internal surface appearances, and sensory qualities of bread in order to determine the optimal ratio of CSPP in the formulation. As CSPP content increased, pH of dough, specific volume, baking loss, and lightness of bread decreased, while total titratable acidity of dough, pH, total titratable acidity, moisture content, and redness of bread increased. Fermentation power of dough expansion increased as incubation time increased. The CSPP samples had significantly higher yellowness, hardness, cohesiveness, gumminess, chewiness, and resilience than the control group. Adhesiveness was the highest at the 12% substitution level, while the lowest at the 6% level. Springiness increased with increasing CSPP content. In the sensory evaluation, as CSPP content increased, scores for color and consistency of crumb decreased, while scores for aroma of sweet pumpkin, sweetness, and delicious taste increased. Density of crumb pore were maximal with the 12% CSPP substitution. The CSPP samples had significantly higher uniformity of crumb pore and springiness of crumb than the control group. However, mouth-feel and overall acceptability showed the reverse effect, obtaining fairly good scores. In conclusion, the results indicate that substituting $6{\sim}9%$ CSPP to hard roll bread is optimal, providing good physiological properties as well as reasonably high overall acceptability.

Leaching of $^{54}Mn$, $^{60}Co$, $^{85}Sr$ and $^{137}Cs$ Deposited to Paddy Soil during the Growing Season of Rice (벼 재배기간 중 논 토양에 처리한 $^{54}Mn$, $^{60}Co$, $^{85}Sr$, $^{137}Cs$의 용탈)

  • Choi, Yong-Ho;Jo, Jae-Sung;Lee, Chang-Woo;Chung, Kyu-Hoi;Lee, Jeong-Ho
    • Korean Journal of Environmental Agriculture
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    • v.15 no.2
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    • pp.198-206
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    • 1996
  • Leaching of $^{54}Mn$, $^{60}Co$, $^{85}Sr$ and $^{137}Cs$ in paddy soil was studied using lysimeter cultures in a greenhouse. The radionuclides were applied to the water surfaces shortly before transplanting and five different times between transplanting and harvest. Fertilizer KCl and slaked lime were added simultaneously in the rate of 83kg and 200kg, respectively, per l0a following the first application after transplanting. Percolating water was sampled until two days before harvest. Concentrations of the radionuclides in percolating water decreased in the order of $^{85}Sr$ > $^{54}Mn$ > $^{60}Co$ > $^{137}Cs$ on the whole. Time taken to reach the maximum was the shortest for $^{137}Cs$(< one week) and the longest for $^{54}Mn$ and $^{85}Sr$. Six days' water dropping started 47 days after transplanting reduced the concentrations of $^{54}Mn$, $^{60}Co$, $^{85}Sr$ and $^{137}Cs$ by factors of 30-180, 3-75, 2-4 and 3-6, respectively, depending on the application time. After the significant decrease, $^{54}Mn$ concentration tended to gradually increase but $^{137}Cs$ did to the contrary Percent leaching varied 0.09-6.2% for $^{54}Mn$, 0.009-0.9% for $^{60}Co$, 1.4-14.4% for $^{85}Sr$ and 0.002-0.06% for $^{137}Cs$, with the application time. The highest leaching came from the application at 40 days after transplanting for all the radionuclides. The addition of KCl and lime increased percent leaching of the radionuclides by factors of 9, 85, 4 and 9, respectively.

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Study on the Geochemical Weathering Process of Sandstones and Mudstones in Pohang Basin at CO2 Storage Condition (지중저장 조건에서 초임계CO2에 의한 포항분지 사암과 이암의 지화학적 풍화반응 연구)

  • Park, Jinyoung;Lee, Minhee;Wang, Sookyun
    • Economic and Environmental Geology
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    • v.46 no.3
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    • pp.221-234
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    • 2013
  • Laboratory experiments for the reaction with supercritical $CO_2$ under the $CO_2$ sequestration condition were performed to investigate the mineralogical and geochemical weathering process of the sandstones and mudstones in the Pohang basin. To simulate the supercritical $CO_2$-rock-groundwater reaction, rock samples used in the experiment were pulverized and the high pressurized cell (200 ml of capacity) was filled with 100 ml of groundwater and 30 g of powdered rock samples. The void space of the high pressurized cell was saturated with the supercritical $CO_2$ and maintained at 100 bar and $50^{\circ}C$ for 60 days. The changes of mineralogical and geochemical properties of rocks were measured by using XRD (X-Ray Diffractometer) and BET (Brunauer-Emmett-Teller). Concentrations of dissolved cations in groundwater were also measured for 60 days of the supercritical $CO_2$-rock-groundwater reaction. Results of XRD analyses indicated that the proportion of plagioclase and K-feldspar in the sandstone decreased and the proportion of illite, pyrite and smectite increased during the reaction. In the case of mudstone, the proportion of illite and kaolinite and cabonate-fluorapatite increased during the reaction. Concentration of $Ca^{2+}$ and $Na^+$ dissolved in groundwater increased during the reaction, suggesting that calcite and feldspars of the sandstone and mudstone would be significantly dissolved when it contacts with supercritical $CO_2$ and groundwater at $CO_2$ sequestration sites in Pohang basin. The average specific surface area of sandstone and mudstone using BET analysis increased from $27.3m^2/g$ and $19.6m^2/g$ to $28.6m^2/g$ and $26.6m^2/g$, respectively, and the average size of micro scale void spaces for the sandstone and mudstone decreased over 60 days reaction, resulting in the increase of micro pore spaces of rocks by the dissolution. Results suggested that the injection of supercritical $CO_2$ in Pohang basin would affect the physical property change of rocks and also $CO_2$ storage capacity in Pohang basin.

Influence of Various Root Media in Pot Growth of 'Seolhyang' Strawberry on the Growth of Daughter Plants and Early Yield after Transplant ('설향' 딸기 포트육묘를 위한 혼합상토 종류가 자묘의 생육과 정식 후 초기수량에 미치는 영향)

  • Park, Gab Soon;Kim, Yeoung Chil;Ann, Seoung Won;Kang, Hee Kyoung;Choi, Jong Myung
    • Horticultural Science & Technology
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    • v.33 no.2
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    • pp.219-226
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    • 2015
  • The objective of this research was to investigate the influence of various root media on the growth of mother and daughter plants during propagation and early yield after transplanting of 'Seolhyang' strawberry. To achieve this, daughter plants were fixed to connected small pots that contained expanded rice-hull (ERH), a strawberry-specialized commercial medium (SSCM), soil mother materials (SMM), or loamy sand (LS). Then, growth of daughter plants in above- and below-ground tissue as well as early yield after transplanting to plastic house soil were investigated. The growth of daughter plants in terms of plant height, leaf area and fresh weight were the highest in the SSCM treatment. Root growth in terms of the amount of primary roots and root dry weight were the highest in the treatments of ERH and SMM and the lowest in that of SSCM, among treatments tested. The ERH treatment also showed the highest values among treatments in root length, surface area and volume when roots with 0 to 0.4 mm in diameter were investigated. The flower bud differentiation of daughter plants began on Sept. 3 in the ERH treatments, earlier than the SMM (Sept. 5) and in SSCM (Sept. 7) treatments. The tissue N contents of daughter plants were in the range of 1.41 to 1.55% in all treatments, and no significant differences were observed among treatments. This indicates that the low moisture retention capacity of ERH and water stress, rather than tissue N contents, promote the flower differentiation of daughter plants. In the evaluation of early yield after transplant, the ERH treatment of showed the highest yield in the period from November to December, reaching 667 g fruit weight per 10 plants. The yields per 10 plants in the other treatments were 581 g in SMM, 475 g in SSCM and 295 g in LS. Above results imply that the various root media have different effects on the growth of daughter plants as well as flower bud differentiation. Therefore, improvement in early yield after transplant can be achieved through selection of proper root medium for daughter plant propagation.