Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Optimization of Extraction Conditions for Useful Components from Sweet Potato Leaves

고구마 잎의 유용성분 추출조건 최적화

  • Li, Meishan (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Jang, Gwi Yeong (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Lee, Sang Hoon (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Kim, Sung Tae (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Lee, Ji Hyun (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Hwang, Se Gu (Department Crop Science, Chungbuk Agricultural Research and Extension Service) ;
  • Sin, Hyun Man (Department Crop Science, Chungbuk Agricultural Research and Extension Service) ;
  • Kim, Hong Sig (Department of Crop Science, Chungbuk National University) ;
  • Kang, Tae Su (Department of Biofood Science Biotechnology, Chungbuk Provincial University of Science and Technology) ;
  • Jeong, Heon Sang (Department of Food Science and Biotechnology, Chungbuk National University)
  • ;
  • 장귀영 (충북대학교 식품생명공학과) ;
  • 이상훈 (충북대학교 식품생명공학과) ;
  • 김성태 (충북대학교 식품생명공학과) ;
  • 이지현 (충북대학교 식품생명공학과) ;
  • 황세구 (충북농업기술원 작물연구과) ;
  • 신현만 (충북농업기술원 작물연구과) ;
  • 김홍식 (충북대학교 식물자원학과) ;
  • 강태수 (충북도립대학 바이오식품생명과학과) ;
  • 정헌상 (충북대학교 식품생명공학과)
  • Received : 2014.07.31
  • Accepted : 2014.10.05
  • Published : 2014.11.30
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Abstract

This study was performed to establish extraction conditions for useful components from sweet potato leaves. Extraction conditions were heating temperature ($40{\sim}60^{\circ}C$), ratio of solvent to sample (10~30 mL/g), and ethanol concentrations (50~90%). The maximum lutein content was $381.88{\mu}g/g$ under the following conditions: 81.23% ethanol concentration, $46.75^{\circ}C$ temperature, and 25.78 mL/g ratio of solvent. The maximum total polyphenol content was 58.44 mg GAE/g under 67.0%, $53.90^{\circ}C$, and 29.10 mL/g, whereas maximum total chlorophyll content was 769.14 mg/100 g under 93.10%, $69.90^{\circ}C$, and 25.98 mL/g, respectively. The highest antioxidant activity of electron-donating ability ($IC_{50}$) was 0.582 mg/mL under 64.56%, $54.63^{\circ}C$, and 19.57 mL/g, whereas total antioxidant activity was 32.54 mg AA eq/g under 64.42%, $44.51^{\circ}C$, and 26.01 mL/g, respectively. Under these conditions, experimental data matched well with the predicted data. Consequently, the present study was carried out to simulate and optimize sweet potato leaves for useful component extraction.

본 연구는 고구마 잎의 유용성분 추출을 최적화하기 위하여 추출온도, 시료에 대한 용매 비 및 에탄올 농도에 대한 영향을 살펴보았다. 추출조건별 lutein, total chlorophyll, polyphenol 및 항산화활성에 대한 반응표면 회귀식의 결정계수 $R^2$ 값은 0.9354~0.9971 범위로 모두 0.1%의 유의수준에서 유의성이 인정되었다. 추출물의 lutein과 총 폴리페놀함량은 온도, 시료에 대한 용매 비 및 에탄올 농도의 영향을 많이 받으며, 총 클로로필 함량은 온도의 영향을 받지 않는 것으로 나타났다. 항산화활성 또한 온도의 영향보다 시료에 대한 용매 비와 에탄올 농도의 영향을 크게 받는 것으로 나타났다. 최적 추출조건과 함량은 lutein이 $46.75^{\circ}C$, 25.78 mL/g 및 81.23%에서 $381.88{\mu}g/g$, 총 폴리페놀은 $53.90^{\circ}C$, 29.10 mL/g 및 67%에서 58.44 mg/g, 총 클로로필은 $69.90^{\circ}C$, 25.98 mL/g 및 93.10%에서 769.1 mg/100 g이었다. DPPH 라디칼 소거능의 경우 $IC_{50}$ 값이 $54.63^{\circ}C$, 19.57 mL/g 및 64.56%에서 0.582 mg/mL로 나타났고, ABTS 라디칼 소거활성은 $44.51^{\circ}C$, 26.01 mL/g 및 64.42 %에서 32.54 mg AA eq/g이었다. 예측된 최적조건에서의 실험값은 예측 값과 유사한 값을 나타내었으며, 이러한 결과는 고구마 잎을 이용한 기능성식품 개발에 이용할 수 있을 것으로 판단된다.

Keywords

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