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Spectroscopic Analysis of Partially Folded State of Ubiquitin (유비퀴틴 단백질의 부분적으로 폴딩된 구조에 대한 분광학적 분석)

  • Park, Soon-Ho
    • Applied Biological Chemistry
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    • v.46 no.4
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    • pp.305-310
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    • 2003
  • Hydrophobic core variant of ubiquitin appeared to have partially folded structure at pH around 2. The intrinsic tryptophan fluorescence emission maximum of this ubiquitin variant at pH 2 showed slight blue shift compare to that of unfolded state, suggesting that some residual tertiary structures remain in this solvent condition. At the same solvent condition, this ubiquitin variant binds with hydrophobic dye, 8-anilinonaphthalene-1-sulfonic acid(AMS), which is known to bind to exposed hydrophobic surface. Furthermore, far-UV circular dichroic spectrum of this ubiquitin variant in the diminished pH was remarkably different from the far-UV CD spectrum of the native state or unfolded state. Based on the molar ellipticity at 220 nm, this ubiquitin variant at pH 2 appeared to have significant amount of secondary structures. All these observations suggest that this ubiquitin variant in the diminished solvent pH has loosely folded hydrophobic core with some secondary structures, which are key features of molten globule conformation. Since molten globule has long been considered as a protein folding intermediate, it is considered that this hydrophobic core variant ubiquitin will serve as a valuable model to study protein folding process.

An Exploratory Study on the Factors of Parental Rearing Behavior Based on Vygotsky's Theory of Social Constructivism for Developing Convergence Digital Contents (융복합 디지털 콘텐츠 개발을 위한 비고츠키의 사회적구성주의 이론에 따른 부모양육행동 요인 탐색)

  • Cha, Hyeon-Ju;Cho, Hee-Sun
    • Journal of Digital Convergence
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    • v.14 no.3
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    • pp.453-464
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    • 2016
  • This study started from the basis of necessity on the researches which asserts that parental rearing behavior in the modern society should change reflecting the social change of sociocultural environment. For this, parental rearing behavior of social constructivism and existing parental rearing behavior were contemplated while construct of parental rearing behavior was explored on the basis of main causes from Vygotsky's theory in order to prepare paradigm of new parental rearing behavior. As far as the method of study goes, various literature from relevant scholars were analyzed, centering various previously conducted studies pertaining to parental rearing behaviors as well as Vygotsky's theory. Also, the validity of the contents was confirmed through consult and revision from three experts. As the result, five factors were extracted. First, factor of cooperative interaction on social interaction. Secondly, emphasis on Internalization factor on social constructive behavior. Thirdly, potential development factor in zone of proximal development. Fourthly, instructional conversation factor as semiotic medium. Lastly, assistance factor as scaffolding. As a result, new paradigm of parental rearing behavior was presented and opened a prospect in developing the touchstone of parental rearing behavior pursuant to social constructivism.

A Multi-Degree of Freedom Measurement System for Determining Geometric Errors in Miniaturized Machine Tool (소형공작기계의 기하학적 오차 평가를 위한 다자유도 측정시스템)

  • S. H., Kweon;Y., Liu;S. H., Yang
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2004.10a
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    • pp.638-643
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    • 2004
  • 소형화된 기계가공시스템은 사용재료의 다양화와 에너지 및 공간의 감소와 같은 장점을 가지고 작고 정밀한 부품을 가공할 수 있는 시스템으로 주목받고 있다. 이러한 시스템이 비록 그 크기가 일반적인 가공시스템에 비해 작지만 정렬 및 조립공정, 기계요소의 불완정성에 의한 기하학적 오차는 여전히 존재한다. 기하학적 오차 평가는 기계시스템의 정밀도를 효과적으로 적은 비용으로 향상시킬 수 있는 오차보정기술을 적용할 수 있는 토대가 된다. 일반적으로, 3 축의 직선축으로 이루어진 공작기계는 21 개의 오차요소를 가진다. 레이져간섭계는 이러한 오차요소를 평가하는데 널리 사용되고 있지만 광학계를 정렬하고 설치하는 데 많은 어려움이 있으며 한번의 설치로 한 개의 오차요소만이 측정 가능하다. 또한, 소형공작기계의 경우, 그 크기로 인해 기존의 레이져 간섭계를 직접적으로 적용할 수 없다. 따라서, 본 연구에서는 소형공작기계를 포함한 소형가공시스템의 기하학적 오차 평가를 위한 새로운 다자유도 측정시스템을 제안하였다. 5 개의 정전용량변위센서를 사용하는 이 시스템을 통해 한 축의 움직임에 따른 5 개의 오차요소를 동시에 측정 가능하다. 균질 변환행렬을 이용한 측정알고리듬을 구성하고 이를 모의시험을 통해 평가하였다. 수학적 모델링을 통해 각 센서의 출력값을 유도하고 이를 이용하여 각 오차요소를 계산하기 위한 식을 유도하였다. 여기서, 단순화된 식을 적용한 경우, 임의의 오차에 대한 측정 알고리듬의 정확도를 평가하였다. 또한, 측정 시스템의 설치시 발생하는 셋업오차에 대한 측정 알고리듬의 민감도 분석을 행하였다. 제안하는 측정 시스템은 구조가 간단하고 고가의 부가장비가 필요치 않다. 또한, 적은 비용으로 구성할 수 있으며 높은 측정 정밀도를 가지고 소형가공시스템에 필요한 오차 평가를 행할 수 있다.가 함유된 계란을 생산하고 섭취하였을 때 특정항체들의 결합을 통해 병원성 미생물의 성장이나 군체를 형성하는 것을 무력화시켜 결과적으로 병원균을 감소시키거나 억제시킨다는 점이다. 오늘날 약물에 내성을 지닌 박테리아의 출현으로 질병감염을 막는데 항생제의 사용효과가 점차 감소하고 있기 때문에 이러한 항생제를 대체할 수 있는 방안으로 계란항체를 이용할 수 있다.한 중공 플랜지 형상의 단조 방법 중 보다 적절한 단조방법인 압조 단조에 있어서 일반적으로 사용되고 있는 SM10C에 대한 유한요소 해석을 수행하였으며, 제품의 형상비에 따라 폴딩 결함의 발생 유무를 검토하고, 폴딩 결함 없이 단조하기 위한 중공 플랜지의 형상한계 비를 제시하였다.도 경미하게 나타났으나, 경련이 나타난 쥐에서는 KA만을 투여한 흰쥐와 구별되지 않았다. 이상의 APT의 항산화 효과는 KA로 인한 뇌세포 변성 개선에 중요한 인자로 작용할 것으로 사료되나, 보다 명확한 APT의 기전을 검색하고 직접 임상에 응응하기 위하여는 보다 다양한 실험 조건이 보완되어야 찰 것으로 생각된다. 항우울약들의 항혈소판작용은 PKC-기질인 41-43 kD와 20 kD의 인산화를 억제함에 기인되는 것으로 사료된다.다. 것으로 사료된다.다.바와 같이 MCl에서 작은 Dv 값을 갖는데, 이것은 CdCl$_{4}$$^{2-}$ 착이온을 형성하거나 ZnCl$_{4}$$^{2-}$ , ZnCl$_{3}$$^{-}$같은 이온과 MgCl$^{+}$, MgCl$_{2}$같은 이온종을 형성하기 때문인것 같다. 한편 어떠한 용리액에서던지 NH$_{4}$$^{+}$의 경

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Improving the ability of oral communication skills for NCO candidates (국가안보에 적합한 인력양성을 위한 부사관 후보생의 의사표현 능력 향상 방안 연구)

  • Song, Jun-Hwa;Cheoni, Myeong Hy
    • Convergence Security Journal
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    • v.14 no.6_1
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    • pp.33-43
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    • 2014
  • In this study, diagnosis of the problem of self-directed learning for the improvement of the language, reasoning n eeded for NCOs typical for students currently enrolled as a cadet NCOs and suggested some lessons to improve this model. Proposed presentation, the three curriculum of presentation and discussion related to the configuration intervie w, self-directed learning was to be his students. In one of the ways to solve the problems experienced in a self-pac ed learning learner was used in this process is often the scaffolding system, is shown that can lead to improved abil ity to apply the result of expression of the three class model.

A Study on Learning Style and Geography Subject Matter (학습스타일과 지리교과 내용특성)

  • 장의선
    • Journal of the Korean Geographical Society
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    • v.39 no.1
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    • pp.132-152
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    • 2004
  • The critical point in this research is that the research on the phenomenon "teaching geography" should include how various elements consisting of the phenomenon are interrelated with each other in diverse angles, not deal with only teaching methods. This research focused on the relationships of the three components of teaching geography : contents of geography subject matter; learner; and scaffolding. Firstly, the characteristics of contents of geography subject matter were analyzed. Geographical knowledge was classified into four categories based on the way of perception. And then the selected geographic contents for this study were done didactic transposition into materials for geography education. These can be presented in a specific classification system from a context of geography education. Secondly, four categories of learning styles were divided by the way learners perceive and process information : Diverger; Assimilator; Converger; Accommodator. Each was connected with learner′s preferred contents of geography subject matter. The correlation between divergers and typical CulturalㆍHistorical Geography and Environmental Geography was high. So was between assimilators and typical Physical Geography and UrbanㆍEconomic Geography. Learners of Converger style tend to prefer GIS and Cartography. Finally, Regional Development and Regional Environmental Problems were highly correlated with accommodators.

A Study on the Application of Perry's Epistemological Development Scheme in Mathematics Education (Perry의 인식론적 신념 발달도식의 수학교육 적용 방안 연구)

  • Yi, Gyuhee;Lee, Jihyun;Choi, Younggi
    • Journal of Educational Research in Mathematics
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    • v.26 no.4
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    • pp.683-700
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    • 2016
  • The traditional teaching-learning in mathematics, which pursue only one correct answer, should be reexamined to cope with an age of uncertainty. In this research, Perry's epistemological development scheme was noticed as a theoretical approach to diagnose problems of dualistic mathematics lessons and to search solutions of the problems. And Design-Based Research method was adopted, We developed the epistemological development scheme through considering Perry's theory and related studies, scaffoldings and teaching-learning to enhance students' epistemological positions in mathematics. Based on these discussions we designed teaching experiment about operations with negative numbers, and analyzed its didactic implications.

Effects of Pre-service Teacher's Scaffolding in Environmental Camp about Climate Change (예비 교사의 스캐폴딩을 강조한 기후 변화 환경 캠프의 효과 분석)

  • Ju, Eun-Jeong;Lee, Jeong-A;Jang, Shin-Ho
    • Journal of Korean Elementary Science Education
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    • v.32 no.1
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    • pp.82-94
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    • 2013
  • The purpose of this study was to investigate the process and the effect of pre-service teacher's scaffolding in environmental camp program about global climate change. For this study, developed the environmental camp program based pre-service teacher's scaffolding and applied to 78 $5^{th}$ students. We analyzed the role of pre-service teacher in the process of scaffolding. In the result, the pre-service teachers conducted cognitive scaffolding like as "Focus", "Hint", "Tell or Summarize" and "Technical Help". They carried out the emotional scaffolding like as "Create Cheerful Atmosphere", "Encourage", and "Help in Living". Teaching and learning about global climate change, the theme of the camp, was regarded uncertain and complex. So, pre-service teacher's scaffolding was effective to promote environmental literacy about climate change of primary students (<0.05). The student teachers understanded the characteristics of the children through emotionally close relationships. The primary students were learned easier about global climate change through cognitive and emotional scaffolding. They experienced environmental practice with communal living in camp.

2012 KOREA STAR AWARDS (지상전시 - 2012미래패키징 신기술 정부포상)

  • (사)한국포장협회
    • The monthly packaging world
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    • s.230
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    • pp.92-99
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    • 2012
  • KOREA STAR AWARDS는 패키징 $\triangle$완제품 $\triangle$재료(플라스틱, 종이 판지, 금속, 유리, 목재, 복합소재) $\triangle$친환경 $\triangle$기계(설비) 및 관련 부품 $\triangle$ 인쇄(라벨링) $\triangle$부자재 $\triangle$생산 및 가공공정 $\triangle$패키징 디자인 등의 분야에서 신기술 개발과 지속가능성을 통해 패키징 산업 발전에 기여한 기업, 그리고 패키징 관련 정책연구, 기술개발, 패키징산업 육성에 기여한 개인에게 시상. 2012 KOREA STAR AWARDS는 코리아스타상(기업, 학생, 공로부문)으로 지식경제부장관상(상장4점, 표창2점), 한국생산기술연구원장상(상장 12점, 표창1점), 한국포장기술사회장상(상장 20점) 총 39점 시상. 코리아스타상 기업부문 지식경제부장관상은 '에이스기계 종이상자 자동접착기의 패스트 폴딩 장치', 'LG생활건강 이자녹스 SMART 진동시리즈', '삼성전자 냉장고 친환경 재사용 포장', '한국에이버리 친환경 수분리성 점착라벨' 4개 제품 수상. 코리아스타상 공로부문 지식경제부장관표창은 (주)경연전람 김영수 대표와 김수일포장개발연구소 김수일 소장 수여. 본 고에서는 "2011 미래패키징 신기술 정부포상" 수상작들의 패키징 동향 및 유공자들의 공적 내용을 살펴보도록 한다.

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Finite Element Analysis for the Forging Process Design of a Blind Rivet (블라인드 리벳의 단조공정설계를 위한 유한요소해석)

  • Byun, Hong-Seok
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.10
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    • pp.2577-2582
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    • 2009
  • A rivet which can fasten two parts is one of an important mechanical elements. In this study, the process design of a blind revet is implemented using finite element method in order to manufacture it which can resist high vibration and has strong coherence between two parts. Considering plastic flow, ease of manufacture, high strength, material loss, and so forth, an optimal four-stage process is proposed by finite element analysis and process design rules. In addition, the finite element simulation results such as shape of the forged rivet, strain distribution and forging load are investigated for the usefulness of the forging process of the blind rivet. These results will be contributed to the forging process design and the die design of the blind rivet.

Area-Efficient Squarer and Fixed-Width Squarer Design (저면적 제곱기 및 고정길이 제곱기의 설계)

  • Cho, Kyung-Ju
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.48 no.3
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    • pp.42-47
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    • 2011
  • The partial product matrix (PPM) of a parallel squarer is symmetric. To reduce the depth of PPM, it can be folded, shifted and rearranged. In this paper, we present an area-efficient squarer design method using new partial product rearrangement. Also, a fixed-width squarer design method of the proposed squarer is presented. By simulations, it is shown that the proposed squarers lead to up to 17% reduction in area, 10% reduction in propagation delay and 10% reduction in power consumption compared with previous squarers. By using the proposed fixed-width squarers, the area, propagation delay and power consumption can be further reduced up to 30%, 16% and 28%, respectively.