• The Korean Journal of Mycology
• /
• v.44 no.2
• /
• pp.103-107
• /
• 2016

To establish a rapid and accurate detection of Phytophthora pinifolia, which is a quarantine pathogenic fungus in Korea, a species-specific primer was developed based on the ras-related protein (Ypt1) gene. Species-specific primer based on the DNA sequences of Ypt1 gene amplified 193 bp polymerase chain reaction (PCR) product for P. pinifolia. The primer pair yielded the predicted PCR product size exactly in testing with target pathogen DNAs, but not from the other 10 species of Phytophthora and 14 species of other phytopathogenic fungi. The primer pair also showed only the species-specific amplification curve on realtime PCR on target pathogen DNA. The detection sensitivity of real time PCR using species-specific primer pair was 10 to 100 times higher than conventional PCR, with 1 to $10pg/{\mu}L$.

• Journal of Animal Science and Technology
• /
• v.45 no.2
• /
• pp.169-182
• /
• 2003

To understand the structure and regulation of bovine ADRP (Adipocyte Differentiation Related Protein) gene, we have isolated the genomic clone of bovine ADRP and determined its sequence. A genomic Southern blot analysis confirmed that ADRP gene is present as a single copy in bovine genome and the ADRP gene spans 12 kb. Bovine ADRP genomic clone, HwADRPg-1, had 8 exons and 7 introns, and all splicing sites conformed to the GT/AG rule with the exon-intron boundaries located exactly. Analysis of the upstream 649 bp of the sequence of HwADRPg-1 showed that it does not contain any canonical TATAA boxes; however Sp1 binding sites and CAAT boxes are found. The promoter contained potential binding sites for AP-1, AP-2 and several putative transcription factor binding sites. The 5'-flanking region of HwADRPg-1 contained muscle specific transcription activator Myo G and C/EBP (CCAAT/ enhancer binding protein) recognizing site. These results suppose that the Myo G transcription activator regulate the transcription of bovine ADRP gene in muscular tissue and its transcriptional activity was triggered by degree of muscular development. Our results provide the necessary analysis for other flanking sequences are needed in addition to the proximal cis elements of this promoter to confer adipocyte differentiation-dependent or growth-dependent transcriptional control.

• Journal of the Korea Society of Computer and Information
• /
• v.11 no.2 s.40
• /
• pp.25-33
• /
• 2006

We propose a method that automatically classifies the images into the object and non-object images. An object image is the image with object(s). An object in an image is defined as a set of regions that lie around center of the image and have significant color distribution against the other surround (or background) regions. We define four measures based on the characteristics of an object to classify the images. The center significance is calculated from the difference in color distribution between the center area and its surrounding region. Second measure is the variance of significantly correlated colors in the image plane. Significantly correlated colors are first defined as the colors of two adjacent pixels that appear more frequently around center of an image rather than at the background of the image. Third one is edge strength at the boundary of candidate for the object. By the way, it is computationally expensive to extract third value because central objects are extracted. So, we define fourth measure which is similar with third measure in characteristic. Fourth one can be calculated more fast but show less accuracy than third one. To classify the images we combine each measure by training the neural network and SYM. We compare classification accuracies of these two classifiers.

• Journal of Sericultural and Entomological Science
• /
• v.53 no.1
• /
• pp.44-49
• /
• 2015

We characterized tissue specific-expressed genes in the posterior silk gland of Bombyx mori using by the Annealing Control Primer based differential display-PCR manner. In this study, we isolated 34 differentially expressed PCR amplicons, which one of these was identified as a novel transcript named as ACP-16 (366 bp), its expression was observed only in the posterior silk glands by Northern blot analysis. To determine promoter region of the ACP-16, we isolated and analyzed a phage DNA having 1.7 kb-long genome DNA including the open reading flame and 5'- upstream untranslated region of the ACP-16 gene from a genomic DNA library. We have estimated a promoter region of the ACP-16 gene by a web promoter prediction engine, which locates -750 ~ -165 from translation initiation site (ATG, +1). ACP-16 gene is necessary to more studies about critical biological role in order to apply the silkworm's transgenic system.

• Computational Structural Engineering
• /
• v.9 no.4
• /
• pp.181-187
• /
• 1996

This paper concerns the singular thermal stresses at the interface corner between the elastic fiber and the viscoelastic matrix of a two-dimensional unidirectional laminate model induced during cooling from cure temperature down to room temperature. Time-domain boundary element method is employed to investigate the nature of residual thermal stresses at the interface. Numerical results show that very large stress gradients are present at the interface corner and such stress singularity might lead to local yielding or fiber-matrix debonding.

• Proceedings of the Korea Information Processing Society Conference
• /
• 2009.04a
• /
• pp.173-176
• /
• 2009

본 논문에서는 감시시스템이 갖추어진 환경 내에서 발생할 수 있는 특이 행동을 효율적으로 감지하기 위한 기법을 제시한다. 최근 대형 범죄 및 방화 사건 등의 방지목적으로 DVR 의 단순 녹화를 벗어나 지능형 감시시스템을 도입하려는 연구가 활발히 진행되고 있다. 그러나 이러한 시스템들은 아직 초기 연구 단계에 있으며 영상내의 관심물체 추출을 위한 전경과 배경의 분리 및 추적 단계에 그치고 있다. 이에 본 논문에서는 가우시안 혼합 모델을 통하여 전경과 배경을 분리하고, 관심영역에 한해서 Optical Flow 기법을 이용하여 폭력상황과 같은 특이 행동의 감지 여부를 판단 할 수 있는 방법에 대해 실험을 통해 평가하였다.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.16 no.12 s.103
• /
• pp.1246-1254
• /
• 2005

Theoretical investigation is made on the electromagnetic fields generated by an impulsive point current source, fur the VV, HV, and VH problems at the interface between an isotropic upper half-space medium and a normally uniaxial lower half-space medium. The electric fields of these problems are associated only with the extraordinary-wave components in the Fourier-Laplace domain. Applying the Cagniard-de Hoop method to each problem, the time-domain solutions of the wave fields are obtained. The fields of the VV case can be expressed in explicit(integral-free) forms. The fields of the HV and VH cases are not integral-free, but the major singularities implicit in the integral solutions can be analytically extracted. The interfacial far fields in the frequency domain are determined by the singularities in the time domain.

• Journal of Korean Society of Steel Construction
• /
• v.12 no.4 s.47
• /
• pp.363-374
• /
• 2000

An accurate method is presented for vibrations of rhombic plates having three different combinations of clamped, simply supported, and free edge conditions. A specific feature here is that the analysis explicitly considers the moment singularities that occur in the two opposite corners having obtuse angles of the rhombic plates. Stationary conditions of single-field Lagrangian functional are derived using the Ritz method. Convergence studies of frequencies show that the corner functions accelerate the convergence rate of solutions. In this paper, accurate frequencies and normalized contours of the vibratory transverse displacement are presented for highly skewed rhombic plates, so that a significant effect of corner stress singularities nay be understood.

• Computational Structural Engineering
• /
• v.10 no.2
• /
• pp.131-138
• /
• 1997

This paper concerns the stress singularity at the interface corner of the viscoelastic adhesive layer bonded between rigid adherends, subjected to a uniform transverse tensile strain. The characteristic equation is derived in the Laplace transformed space, following Williams, and the transformed characteristic equation is inverted analytically into real time space for the viscoelastic model considered here. The order of the singularity is obtained numerically. The time-domain boundary element method is employed to investigate the nature of stresses along the interface. Numerical results show that the order of the singularity increases with time while the free-edge stress intensity factors are relaxed with time.

• Proceedings of the Optical Society of Korea Conference
• /
• 2002.07a
• /
• pp.164-165
• /
• 2002

광자결정 광섬유(photonic crystal fiber)는 주기적으로 배열된 공기기둥이 광섬유의 길이 방향으로 정렬되어 일반광섬유의 크래팅의 역할을 하고 있고 코어는 이들 공기구멍의 중심부에 인위적인 결함을 만들어 광도파가 가능하게 된다 이러한 광자결정 광섬유의 광학적 특성은 넓은 영역에 걸친 단일 모드 특성, 특이한 모드분산 강한 비선형 등의 기존의 광섬유와는 다른 광특성이 보고되고 있으며 테라 헐즈 펄스 도파가 보고되는 등 그 응용 영역을 넓히고 있다. (중략)