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Bioactive Utility of the Extracts from Rhus verniciflua Stokes (RVS) : Biological Function of the Extracts from RVS (옻나무 추출물의 생리활성 이용에 대한 연구 : 옻나무 추출물의 생물학적 기능)

  • Lim, Kye-Taek;Lee, Jeong-Chae
    • Korean Journal of Food Science and Technology
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    • v.31 no.1
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    • pp.238-245
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    • 1999
  • Antioxidative effects of the water or ethanol extracts from Rhus verniciflua Stokes (RVS) were measured by protection against hydroxyl radicals in mouse brain tissue culture. In the water extracts from RVS, cell viabilities were estimated 60.0, 66.0, 72.0, 84.0 and 90.0% at addition of 1, 2, 4, 7 and $10{\mu}L$, respectively, compared with GO (20 mU/mL) alone. The cell viability in the ethanol extracts was similarly with water extracts. In the antitumor effects, the results showed that percentages of the HeLa cell death were approximately 24% for 12 hrs, 57% for 48 hrs at addition of 10%/well ethanol extracts respectively. To know inhibition of tumor growth, in vivo, mice (BALB/c) were inoculated with 0.25 mL CT-26 $(1{\times}10^6\;cells/mL)$ subcutaneously. After the generation of tumor, the results of RVS extracts (ethanol, water) injection showed generally that the tumor size in BALB/c was reduced. For physicochemical characterization of the RVS extracts, purified substances of water or ethanol extracts were analized with SDS-PAGE and ICP spectrometer. In electrophoresis, gel showed 2 bands (210, 230 KDa). The results of ICP verified that RVS extracts contain $Cu^{2+}$ in both samples. Conclusively, this substance might be a laccase which has a biological effective function, as a natural bioactive substance.

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Determination of Safe Cropping Season in Direct- Seeding of Rice on Flooded Paddy by Using Effective Temperatures in Agroclimatic Zones (농업기후지대별 작물생육 유효기온 출현특성에 따른 벼 담수직파 안전작기 설정)

  • Shim Kyo-Moon;Lee Jeong-Taek;Yun Seong-Ho;Choi Don-Hyang
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.1 no.1
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    • pp.72-80
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    • 1999
  • The study was conducted to establish the safe cropping season for direct- seeding on flooded paddy by the analysis of meteorological data(l973~1992, 20 years) from Korea Meteorological Administration. The critical date for early seeding(CDES) at direct- seeding culture on flooded paddy was decided by the appearance date of daily mean air temperature(DMAT) of 15$^{\circ}C$. The optimum heading date(OHD) was the first day when 22$^{\circ}C$ of daily mean air temperature could be kept for 40 days of ripening period after heading, and the critical date of late heading for safe ripening(CDHR) was the last day when 19$^{\circ}C$ of daily mean air temperature could be kept for 40 days after heading. The optimum seeding date(OSD) and the critical date for late seeding(CDLS) could be decided by the accumulated temperature from OHD and CDHR to the appearance dates of necessary temperatures for early, intermediate, and intermediately late maturing varieties. This results can be used for the determination of the safe cropping season of direct-seeding on flooded paddy in each agroclimatic zone. For instance, the OSD appearance date for early maturing variety in Suwon region appeared to be May 11~20 and the CDLS appearance date was May 31~June 7.

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Fluctuation of the sensitivity of Colletotrichum spp. causing the red-pepper anthracnose to chlorothalonil (살균제 chlorothalonil에 대한 고추 탄저병균의 감수성 변화)

  • Kim, Joon-Tae;Lee, Kyeong-Hee;Min, Ji-Young;Cho, In-Joon;Kang, Beum-Kwan;Park, Seong-Woo;Bach, Nguyen Van;Kim, Yun-Sik;Hong, Seong-Taek;Rho, Chang-Woo;Kim, Heung-Tae
    • The Korean Journal of Pesticide Science
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    • v.8 no.3
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    • pp.231-237
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    • 2004
  • Monitoring for the sensitivity of Colletotrichum spp. causing red-pepper anthracnose to chlorothalonil was conducted by the agar dilution method, which were isolated from infected pepper fruits in 1999 and 2002. Among the isolates in 1999 or 2002, their sensitivity to fungicide was fluctuated. Investigating the inhibitory effect of $500{\mu}g/m\ell$ of chlorothalonil on the mycelial growth of Colletotrichum isolates isolated from the infected red-pepper fruits in 1999, the frequency of isolates was 23.4%, which showed 100% of inhibitory effect on mycelial growth, and 29.7% showing below 60%. Isolates in 2002, however, showed 34.6% and 14.8% of inhibitory effect on PDAs including the same concentration of fungicide, respectively, These results showed that the sensitivity of Colletotrichum isolates in 1999 was inferior to that in 2002. According to the sampling region, isolation frequency among isolates in 2002 showing less MIC rather than 1999 was increased in Kyunggi, Kangwon, Kyungbuk and Kyungnam while the frequency of resistant isolates was done in Chungnam, Chungbuk, Cheonnam and Cheonbuk Fungicide monitoring results in this report will be of use on controlling the anthracnose in each region cultivating red-pepper.

Effects of Rubus Coreanus Miq. Oil on Serum Lipids in C57BL/6J Mice (복분자씨유의 식용유지 대체가 C57BL/6J Mice의 혈청 지질에 미치는 영향)

  • Byun, Moon-Sun;Cha, Youn-Soo;Hwang, Keum-Taek;Yu, Ok-Kyeong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.7
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    • pp.953-960
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    • 2015
  • This study evaluated the effects of Rubus coreanus Miq. oil on the plasma lipid profile of high fat diet (HFD)-induced obese mice. Animals were randomly divided into 4 groups (n=10). After completion of the 5-week experimental period, we measured bodyweight gain, food intake, adipose tissue mass, and plasma lipid profile. We also analyzed the activities of carnitine and superoxide dismutase (SOD) involved in ${\beta}$-oxidation and antioxidation, respectively. Our results show that HFD-induced weight gain in animals in the R. coreanus Miq. oil diet group (RCO) and corn oil diet group (CO) was significantly lower compared to animals in the HFD group; RCO supplementation had a more noticeable effect than CO. Visceral and back fat weights were lower in the RCO and CO groups while plasma HDL cholesterol (HDL-C) and HDL-C per total cholesterol [HDL-C/TC (%)] ratio were significantly higher in the RCO group. The contents of acid-soluble acylcarnitine and total carnitine as well as SOD activation were significantly higher in the RCO group, but no significant difference was observed between the RCO and CO groups. In conclusion, RCO effectively averted elevation of total body weight and fat weight in HFD-induced obese mice and promoted increased HDL-C. Therefore, R. coreanus Miq. oil might play an anti-obesity role in obese people and could be used as an effective oil supplement.

Analysis of Characterization in Commercial Extra Virgin Olive Oils (유통 압착올리브유의 이화학적인 특성)

  • Nam, Ha-Young;Lee, Ki-Teak
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.7
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    • pp.866-873
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    • 2007
  • To analyze and differentiate volatile compounds of 13 extra virgin olive oils from market, solid-phase micro extraction (SPME) GC-MS and electronic nose (EN) equipped with metal oxide sensors were applied. The volatiles identified in extra virgin olive oils include hexanal, 4-hexen-1-ol, (Z)-3-hexen-1-ol, acetic acid, and 2,4-dimethyl-heptane, etc. Response from EN was analysed by the principal component analysis. Proportion of the first Principal component was 99.70%, suggesting that each aroma pattern of the 13 extra virgin olive oils could be discriminated by EN. Fatty acid compositions were oleic (61.1${\sim}$77.9 mole%), palmitic (11.7${\sim}$16.5 mole%), linoleic (4.7${\sim}$9.7 mole%), stearic (2.5${\sim}$2.9 mole%), Palmitoleic (0.8${\sim}$2.4 mole%), and linolenic acid (0.7${\sim}$1.2 mole%). In color study, extra virgin olive oil showed $L^{\ast}$ value of 81.7${\sim}$92.9, $a^{\ast}$ value of -28.3${\sim}$13.5 and $b^{\ast}$ value of 52.2${\sim}$139.0. Total phenol and ${\alpha}-tocopherol$ contents were 6.2${\sim}$24.9 mg/100 g and 5.5${\sim}$12.8 mg/100 g, respectively. In Rancimat test, the induction period of 13 extra virgin olive oils showed 31.76${\sim}$54.04 hr while their POV ranged from 13.5 to 22.9 meq/kg oil.

Determination of Positional Fatty Acid and Triacylglycerol Compositions of Selected Infant Formulas (영유아용 조제분유의 위치별 지방산 및 Triacylglycerols의 정성 분석)

  • Son, Jeoung-Mae;Lee, Jeung-Hee;Hong, Soon-Taek;Lee, Kyung-Su;Park, Hye-Kyung;Kwon, Kwang-Il;Lee, Ki-Teak
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.9
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    • pp.1256-1264
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    • 2011
  • Infant formula is one of the main nutritional sources for infants. In this study, the fatty acid compositions of Korean (domestic, n=8) and foreign infant formulas (n=3) were analyzed. Crude fats were extracted using the Folch method, and fatty acid compositions (total and positional) were analyzed by gas chromatography. In the fatty acid compositions of infant formulas, oleic (19.88~44.64%), palmitic (7.59~21.65%) and linoleic acids (16.72~25.87%) were the major fatty acids. Domestic infant formula products contained 35.94~56.55% total saturated fatty acid content, whereas that of foreign infant formula ranged from 34.40~42.88%. The content of monounsaturated fatty acids ranged from 20.23~44.99% in domestic products and 34.11~45.07% in foreign products. In addition, 0.17~2.57% arachidonic acid/docosahexaenoic acid and 10~13% linoleic acid/linolenic acid were detected in the analyzed products (domestic and foreign products). A small amount of trans fatty acids (0.25~1.69%) were found. In sn-2 position analysis, palmitic acids (1.84~38.74%) were detected in the analyzed formulas. Further, typical triacylglycerols in human milk, including 1,3-di-monounsaturated-2-saturated triacylglycerol, were not detected in the analyzed formulas.

Recent Progress in Air-Conditioning and Refrigeration Research: A Review of Papers Published in the Korean Journal of Air-Conditioning and Refrigeration Engineering in 2008 (설비공학 분야의 최근 연구 동향: 2008년 학회지 논문에 대한 종합적 고찰)

  • Han, Hwa-Taik;Choi, Chang-Ho;Lee, Dae-Young;Kim, Seo-Young;Kwon, Yong-Il;Choi, Jong-Min
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.21 no.12
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    • pp.715-732
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    • 2009
  • This article reviews the papers published in the Korean Journal of Air-Conditioning and Refrigeration Engineering during 2008. It is intended to understand the status of current research in the areas of heating, cooling, ventilation, sanitation, and indoor environments of buildings and plant facilities. Conclusions are as follows. (1) Research trends in thermal and fluid engineering have been surveyed in the categories of general fluid flow, fluid machinery and piping, new and renewable energy, and fire. Well-developed CFD technologies were widely applied in developing facilities and their systems. New research topics include fire, fuel cell, and solar energy. Research was mainly focused on flow distribution and optimization in the fields of fluid machinery and piping. Topics related to the development of fans and compressors had been popular, but were no longer investigated widely. Research papers on micro heat exchangers using nanofluids and micro pumps were also not presented during this period. There were some studies on thermal reliability and performance in the fields of new and renewable energy. Numerical simulations of smoke ventilation and the spread of fire were the main topics in the field of fire. (2) Research works on heat transfer presented in 2008 have been reviewed in the categories of heat transfer characteristics, industrial heat exchangers, and ground heat exchangers. Research on heat transfer characteristics included thermal transport in cryogenic vessels, dish solar collectors, radiative thermal reflectors, variable conductance heat pipes, and flow condensation and evaporation of refrigerants. In the area of industrial heat exchangers, examined are research on micro-channel plate heat exchangers, liquid cooled cold plates, fin-tube heat exchangers, and frost behavior of heat exchanger fins. Measurements on ground thermal conductivity and on the thermal diffusion characteristics of ground heat exchangers were reported. (3) In the field of refrigeration, many studies were presented on simultaneous heating and cooling heat pump systems. Switching between various operation modes and optimizing the refrigerant charge were considered in this research. Studies of heat pump systems using unutilized energy sources such as sewage water and river water were reported. Evaporative cooling was studied both theoretically and experimentally as a potential alternative to the conventional methods. (4) Research papers on building facilities have been reviewed and divided into studies on heat and cold sources, air conditioning and air cleaning, ventilation, automatic control of heat sources with piping systems, and sound reduction in hydraulic turbine dynamo rooms. In particular, considered were efficient and effective uses of energy resulting in reduced environmental pollution and operating costs. (5) In the field of building environments, many studies focused on health and comfort. Ventilation. system performance was considered to be important in improving indoor air conditions. Due to high oil prices, various tests were planned to examine building energy consumption and to cut life cycle costs.

The Effects of Mixtures with Ginseng Radix rubra and Paeonia Radix on Endurance Exercise Performance (홍삼과 백작약의 혼합물 투여가 지구성 운동수행능력에 미치는 영향)

  • Kim, Seung-Kyum;Kim, Chang-Ju;Kim, Hong;Lim, Yong-Taek;Yoon, Sung-Jin
    • Journal of Ginseng Research
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    • v.31 no.2
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    • pp.93-101
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    • 2007
  • The purpose of this study is to examine the improvement of endurance exercise performance by taking mixtures of Ginseng Radix rubra and Paeonia Radix. For this purpose, time of exhaustion, $V0_2max$, heart rate, and blood lactate was used as improvement index. The experiments were executed to thirty eight men in their twenties. They were randomly assigned into four groups[CG: control group(n=10), IG: ingestion mixtures group(n=9), TPG: ingestion placebo+training group(n=9), TIG: ingestion mixtures+training group(n=10)]. IG and TIG took mixtures of Ginseng Radix rubra and Paeonia Radix for four weeks, and TPG and TIG exercised once a day, three times a week, exercised for 25 minutes during the first week and added 2 minutes by week during four weeks up to 75% of their maximum heart rate. Time of exhaustion showed statistically significant increase for IG, TPG and TIG. TIG had the longest time of exhaustion and IG the shortest. In case of $VO_2max$, no statistically significant change was found among four groups. However, IG, TPG, TIG showed small increase in $VO_2max$. Similarly, no significant change was found for heart rate in rest even though TIG and TPG appear to have decreasing trend. For maximum heart rate, no significant change was found either, but IG, TPG and TIG showed increase. In case of blood lactate from rest to exercise until 18 minutes, no group had significant decrease. In case of blood lactate in exhaustion, IG, TPG and TIG had significant increase. Blood lactate recovery volume at 5 minutes, 10 minutes, 20 minutes showed no significant results for all four groups, but TIG and TPG had a small increase.

Characterization of Scaled-up Low-Trans Shortening from Rice Bran Oil and High Oleic Sunflower Seed Oil with Batch Type Reactor (회분식반응기를 이용한 미강유, 팜스테아린과 고올레인산 해바라기씨유 유래 대량 제조된 저트랜스 쇼트닝의 특성 연구)

  • Kim, Ji-Young;Lee, Ki-Teak
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.3
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    • pp.338-345
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    • 2009
  • Scaled-up low-trans shortening (LTS) was produced by lipase-catalyzed interesterification. Blend of rice bran oil (RBO), palm stearin (PS) and high oleic sunflower seed oil (HO) with 1:2:0.9 (w/w/w) ratio was interesterified using immobilized lipase from Thermomyces lanuginosus (TLIM) in the batch type reactor at $65^{\circ}C$ for 24 hr, and physicochemical melting properties of LTS were compared with commercial shortening. Solid fat content (SFC) of commercial shortening (used as control) and LTS was similar at 9.56 and 8.77%, respectively, at $35^{\circ}C$. Major fatty acids in LTS were C16:1 (33.7 wt%), C18:1 (45.7 wt%) and C18:2 (13.4 wt%). Trans fatty acid content in the commercial shortening (4.8 wt%) was higher than that of LTS (0.5 wt%). After reverse-phase HPLC analysis, major triacylglycerol (TAG) species in LTS were POO, POP and PLO. Total tocopherol, ${\gamma}$-oryzanol and phytosterol contents in the LTS were 12.37, 0.43 and 251.38 mg/100 g, respectively. Hardness of LTS was similar to that of commercial shortening. Also, x-ray diffraction analysis showed coexistence of ${\beta}'$ and ${\beta}$ form in the LTS.

Comparison of Population Monitoring Methods for Breeding Forest Birds in Korean Temperate Mixed Forests (국내 온대 혼효림에 서식하는 산림성 조류의 번식기 개체군 모니터링 방법에 대한 비교)

  • Nam, Hyun-Young;Choi, Chang-Yong;Park, Jin-Young;Hur, Wee-Haeng
    • Journal of Korean Society of Forest Science
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    • v.108 no.4
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    • pp.663-674
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    • 2019
  • Birds are effective ecological indicators but there is no national protocol in place to monitor population dynamics of forest birds in Korea. To support the establishment of future monitoring protocols, we compared the results of two generally used monitoring methods for forest bird surveys in two temperate mixed forests in central Korea. There was no statistical difference in the number of species and individuals detected per unit survey effort when comparing line transects and point counts. The number of species and individuals were higher in a five-minute count than in a three-minute point count, but the total accumulated number of expected observed species showed no difference between the two count durations. The number of observed species and individuals increased in both methods as plot radius or transect width increased, suggesting that multi-layer or multi-band surveys may be useful for quantitative and qualitative objectives. The decreasing number of observed species and individuals after sunrise suggested that bird monitoring should be conducted earlier in the morning, within four hours after sunrise. To detect 70% of the total number of species, 7.0 to 7.6 survey hours, equivalent to 42 three-minute counts (95% confidence interval [CI]: 26 to 61) or 33 five-minute counts (95% CI: 19 to 53) were needed for unlimited radius point counts. On the other hand, 4.8 survey hours, equivalent to 26 line transect counts (95% CI: 15 to 45) using 200-m transects with unlimited width, were required to achieve the same level of species detection. Therefore, the line transect method may be more effective than the point count method, at least in terms of local species richness assessment. For national forest bird monitoring, our data indicated that one or both survey methods can be selected as a basic protocol, based on the goals and scales of monitoring, forest types, and the conditions of the target areas.