Fluctuation of the sensitivity of Colletotrichum spp. causing the red-pepper anthracnose to chlorothalonil

살균제 chlorothalonil에 대한 고추 탄저병균의 감수성 변화

  • Kim, Joon-Tae (Dept. of Plant Medicine, College of Agriculture, Chungbuk National University) ;
  • Lee, Kyeong-Hee (Umseong Controlled Agricultural Experiment Station Chungbuk Agricultural Research and Extension Service) ;
  • Min, Ji-Young (Dept. of Plant Medicine, College of Agriculture, Chungbuk National University) ;
  • Cho, In-Joon (Dept. of Plant Medicine, College of Agriculture, Chungbuk National University) ;
  • Kang, Beum-Kwan (Dept. of Plant Medicine, College of Agriculture, Chungbuk National University) ;
  • Park, Seong-Woo (Dept. of Plant Medicine, College of Agriculture, Chungbuk National University) ;
  • Bach, Nguyen Van (Dept. of Plant Medicine, College of Agriculture, Chungbuk National University) ;
  • Kim, Yun-Sik (Dept. of Plant Medicine, College of Agriculture, Chungbuk National University) ;
  • Hong, Seong-Taek (Umseong Controlled Agricultural Experiment Station Chungbuk Agricultural Research and Extension Service) ;
  • Rho, Chang-Woo (Umseong Controlled Agricultural Experiment Station Chungbuk Agricultural Research and Extension Service) ;
  • Kim, Heung-Tae (Dept. of Plant Medicine, College of Agriculture, Chungbuk National University)
  • 김준태 (충북대학교 농과대학 식물의학과) ;
  • 이경희 (충북농업기술원 음성시설농업시험장) ;
  • 민지영 (충북대학교 농과대학 식물의학과) ;
  • 조인준 (충북대학교 농과대학 식물의학과) ;
  • 강범관 (충북대학교 농과대학 식물의학과) ;
  • 박성우 (충북대학교 농과대학 식물의학과) ;
  • ;
  • 김윤식 (충북대학교 농과대학 식물의학과) ;
  • 홍성택 (충북농업기술원 음성시설농업시험장) ;
  • 노창우 (충북농업기술원 음성시설농업시험장) ;
  • 김흥태 (충북대학교 농과대학 식물의학과)
  • Published : 2004.09.30

Abstract

Monitoring for the sensitivity of Colletotrichum spp. causing red-pepper anthracnose to chlorothalonil was conducted by the agar dilution method, which were isolated from infected pepper fruits in 1999 and 2002. Among the isolates in 1999 or 2002, their sensitivity to fungicide was fluctuated. Investigating the inhibitory effect of $500{\mu}g/m\ell$ of chlorothalonil on the mycelial growth of Colletotrichum isolates isolated from the infected red-pepper fruits in 1999, the frequency of isolates was 23.4%, which showed 100% of inhibitory effect on mycelial growth, and 29.7% showing below 60%. Isolates in 2002, however, showed 34.6% and 14.8% of inhibitory effect on PDAs including the same concentration of fungicide, respectively, These results showed that the sensitivity of Colletotrichum isolates in 1999 was inferior to that in 2002. According to the sampling region, isolation frequency among isolates in 2002 showing less MIC rather than 1999 was increased in Kyunggi, Kangwon, Kyungbuk and Kyungnam while the frequency of resistant isolates was done in Chungnam, Chungbuk, Cheonnam and Cheonbuk Fungicide monitoring results in this report will be of use on controlling the anthracnose in each region cultivating red-pepper.

탄저병의 전형적인 병징을 보이는 이병 고추로부터 1999년과 2002년에 128개와 257개의 고추 탄저병균을 분리한 후, chlorothalonil에 대한 감수성 변화를 한천평판희석법을 이용하여 조사하였다. 삼년간의 기간을 두고서 두 해에 걸쳐 채집한 탄저병균의 chlorothalonil에 대한 감수성 정도는 동일한 해에 채집한 균주간에도 큰 차이가 있었다. Chlorothalonil의 농도를 $500{\mu}g/mL$로 조절한 PDA 배지에서 균사생장 억제 효과를 조사하여 보면, 1999년에 채집한 균주들 중에서 균사생장 억제효과가 100%인 균주의 비율은 23.4%, 60% 이하의 억제 효과를 보이는 균주는 29.7%를 차지하는 반면, 2002년에 분리한 균주의 비율은 34.6%와 14.8%로 나타났다. 이러한 결과는 1999년에 분리된 균주가 2002년에 분리된 균주보다는 chlorothalonil에 대하여 저항성이었음을 알 수 있었다. 지역에 따른 chlorothalonil에 대한 탄저병균의 감수성 변화 양상은 강원, 경기도와 경상도 지역에서 분리한 탄저병균은 2002년에 감수성화 되었지만, 충청과 전라도 지역에서 분리한 병원균에서는 저항성을 보이는 균주의 비율이 증가하였다. 본 실험과 같은 살균제에 대한 감수성 모니터링 자료는 지역별로 고추 탄저병의 방제를 위해서 사용하는 살균제를 선발하는 기초자료로 사용할 수 있을 것이다.

Keywords

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