• The Korean Journal of Zoology
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• v.18 no.4
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• pp.181-196
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• 1975

Rabbit follicular oocytes were cultured in a basic medium containing 0.4% bovine serum albumin (BSA), carbohydrates and amino acids in various combinations. Osmolarity of the medium was maintained at 308 mOsm. The carbohydrates, pyruvate, lactate and glucose were all about equally beneficial, but not essential for rabbit oocyte maturation. Glutamine and proline, but not methionine or phenylalanine stimulated oocyte develoment. Glutamine stimulated more follicular oocytes to develop to prophase and metaphase II than did any of the three carbohydrates tested alone or in combination. Ammonia production after 24 hours of culture was highest in medium containing glutamine(15.2$\\mu$g/ml) but this was not inhibitory to maturation. Negligible amounts of ammonia were found with the other amino acids added. The optimum level of osmolarity for rabbit oocyte maturation appears to be ranged from 250$\\sim$310 mOsm with the maximum level of 270 mOsm. With 0, 0.08, 0.4, 2, 10 and 50 mM of glutamine in the medium, plus BSA but without carbohydrates, 30, 73, 70, 71, 59, 45% of the oocytes developed to prophase or metaphase II respectively. This indicates that no carbohydrate is required of the maturation of rabbit oocytes when 0.08$\\sim$2 mM of glutamine is included, which are the optimum range. Follicular oocytes could develop in the medium containing $^14 C$-glutamine and BSA but without carbohydrates or other organic compound. From the $^14 CO_2$ produced and TCA precipitable material isolated, it is suggested that glutamine probably is utilized by oocytes and cumulus cells as a source of energy as well as for protein synthesis.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.7
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• pp.879-885
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• 2008

This study was conducted to analyze 15 traditional Korean gruels for nutrient density using the Index of Nutrient Quality (INQ). The calorie of the gruels ranged from 148.8 (Daechujuk) to 294.1 kcal (Jatjuk) per serving size. The calorie in 'Jatjuk' was the highest, covering 33.3% of Dietary Reference Intakes for Koreans (KDRI) per meal. The carbohydrate content was between 23.0 g (Dakjuk) and 52.1 g (Patjuk) per serving size. The average content of carbohydrate in gruels was $34.7{\pm}9.1\;g per serving size which covers 24.4% of KDRI per meal. Among the 15 gruels, Patjuk contained the highest carbohydrate content (52.1 g per serving size, 36.7% of KDRI per meal). The highest nutrient density (INQ) of carbohydrate in gruels was Dachujuk (INQ 1.4). The protein content of the gruels was between 2.3 (Huinjuk) and 22.3 g (Dakjuk) per serving size. The average content of protein in gruels was 25.6% of KDRI per meal. Dakjuk contained the highest protein content ($8.2{\pm}4.9\;g$ per serving size) and followed by Dakjuk (INQ 2.5), Guljuk (INQ 1.5) and Kongjuk (INQ 1.3) in nutrient density (INQ) of protein. The fat content of the gruels were between 0.3 (Daechujuk) and 17.8 g (Jatjuk) per serving size. The average content of fat in gruels was $17.8{\pm}0.12\;g$ per serving size which showed 21.1% of KDRI per meal. Among the gruels, Jatjuk contained the highest fat ($17.8{\pm}0.12\;g$ per serving size, 77.4% of KDRI per meal). In order of the high nutrient density (INQ) of protein, Jatjuk (INQ 2.4) was followed by Heugimjajuk (INQ 1.5) and Kongjuk (INQ 1.5).

• Journal of Animal Science and Technology
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• v.48 no.4
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• pp.521-532
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• 2006

An in vitro study was conducted to examine the effect of addition level of carbohydrates on fermentation characteristics, and bio-hydrogenation of unsaturated fatty acids by mixed rumen bacteria when incubated with soybean oil or flaxseed oil. Four levels(0%, 0.3%, 0.6% and 0.9%, w/v) of the mixed carbohydrates(glucose, cellobiose, soluble starch, 1:1:1, in weight basis) and oil sources(soybean or flaxseed oil, 60mg in 150ml culture solution) were added to the mixed solution of strained rumen fluid with artificial saliva(1:4, v/v), and incubated anaerobically for 12 hours at 39℃. pH and ammonia-N concentration were lower by increasing the substrate levels at all incubation periods(P<0.05～P<0.001). The propionate proportion increased(P<0.001), but acetic acid and butyric acid decreased(P<0.001) with the substrate level at 6 and 12 h incubations. Oil sources did not influence the proportions of individual VFA. At the end of incubation, the proportions of C18:0(P<0.01), C18:1(P<0.001) and trans-11C-18:1(P<0.01) were reduced but those of C18:2(P<0.001) and C18:3(P<0.01) were enhanced by the addition of flaxseed oil compared to addition of soybean oil. The proportions of C18:0 and total CLA were reduced(P<0.01) but those of trans-11-C18: (P<0.05) and C18:2(P<0.01) were increased with the substrate level when incubated with soybean oil or flaxseed oil. There were interactions(P<0.05) in the proportions of C18:1, C18:2 and C18:3(P<0.01) between oil source and substrate level. The proportions of cis-9, trans-11-CLA and trans-10, cis-12-CLA tended to reduce with substrate level, although there was no significant difference between treatments.

• Journal of the Korea Organic Resources Recycling Association
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• v.25 no.1
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• pp.103-110
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• 2017

In Concentrated Animal Feeding Operations(CAFOs), emission of ammonia from stored manure contributes negatively on the wellness of livestock. In CAFOs facilities, indoor aerial ammonia concentration oftentime surpasses the critical level potentially harmful to livestock's immune system. Understandably, numerous researches to control aerial ammonia have been conducted in countries where CAFOs were practiced for many decades. Some innovative technologies, such as scrubber, bio-filter, and additives emerged, as a result. Among them, microbial additives became popular in Korea, due to an easiness of use and affordability. However, microbial additives still have some weaknesses. Their price is still high enough to discourage farmers who run a small scale farm and their effectiveness are still questioned by many users and researchers. In the present study, we found soluble carbohydrates, such as sugar, glucose, and molasses, when supplemented to pig slurry manure, can mitigate ammonia emission. To be more specific, pig manure slurry(120kg), stored in container(200L), was supplemented with sugar at 0.1%(w/w) and was, subsequently, monitored for pH and aerial ammonia for next 10 days. From this experiment, it was found that the sugar supplementation was effective in mitigating the aerial ammonia concentration (33% in average) when monitored daily. Also, the pH of manure slurry was maintained at relatively low level(8.2) in sugar-supplemented manure slurry while it was elevated to 8.5 in untreated slurry. Conclusively, the obtained data suggest that soluble carbohydrate can mitigate ammonia emission by acidifying manure slurry. Additionally, it can be suggested that soluble carbohydrates, such as sugar, glucose, and molasses, can be reasonable choices for animal farmers who have been looking for an alternative choice to replace expensive microbial additives.

• Journal of Bio-Environment Control
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• v.26 no.4
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• pp.291-296
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• 2017

This study was conducted to evaluate the influence of early defoliation on photosynthesis and carbohydrate reserves when the source leaves of 'Fuji'/M9 apple (Malus domestica Borkh.) trees were removed during the growing period. Bud regrowth rates of 80%, 50% defoliation and non-defoliation treatments were significantly different 82.7%, 45.9% and 2.1% respectively at 30 days after treatment. In all treatments, sucrose and starch concentrations in remaining leaves decreased non-significantly during the 14-day period. No significant changes were observed for total soluble carbohydrates in non-defoliation and 50% defoliation. However, in 80% defoliated treatments, concentrations of sorbitol and total soluble carbohydrates in remaining leaves declined steadily during the 14-day period. It is thought that high sink strength increases the requirements of carbohydrate from remaining leaves more than non-defoliated. The concentrations of starch in the roots tend to decrease non-significantly as percentage of defoliation increased. Photosynthesis of remaining leaves was monitored during the 14-day period after partial defoliation treatments. Net photosynthetic rates (Pn) and stomatal conductance were significantly enhanced in the 80% defoliation. The observed photosynthetic enhancement following partial defoliation may have been due to the enhancement of osmotic potential in leaves. These results were estimated that increasing of photosynthetic rate in the partial defoliation is due to the sink carbohydrate requirements for the current year's secondary growth of buds.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.1
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• pp.44-49
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• 2008

Sufficient inorganic nitrogen supply for crop growth is crucial for economically sustainable organic farming. The effects of an application of cover crop biomass on crop growth, nitrogen utilization and carbohydrate composition were investigated during early stage. Short-term changes in soil nitrogen after incorporating fresh hairy vetch and rye shoots were measured. The inorganic nitrogen from cover crops reached the peak at 15 ($NH_4-N$) and 24 ($NO_3-N$) days after incorporation, and then decreased rapidly. The highest concentration of soil nitrate showed at 27 days of incorporation in hairy vetch and at 18 days in rye, and three fold differences exhibited between two treatments. Crop growth under hairy vetch or rye incorporation significantly differed. At 20 DAT, dry matter production in NPK and hairy vetch was about two fold greater than that in rye. Difference in decomposing rates of hairy vetch and rye had also influence on nitrogen status in leaves and roots of pepper plants. Total nitrogen was greater in NPK and hairy vetch than in rye until 20 DAT, whereas inorganic nitrogen (nitrate and nitrite) concentration was higher in rye. Temporal changes in soluble sugars and starch in pepper plants among treatments were similar, although difference in the amount existed. It was suggested that hairy vetch as an alternative nitrogen source promoted crop growth and mineral utilization during early growth stage, whereas an obvious effect in rye was not found.

• Journal of the Korean Society of Food Culture
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• v.7 no.1
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• pp.65-72
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• 1992

Annual changes of nutrient intakes of Koreans and Japanese were compared on the basis of the data from the National Nutrition Survey which has been carried out annually in Korea (1969-1988) and in Japan (1950-1988) for the guide of the future food and nutrition policy. Intakes of all nutrients except carbohydrate and protein by Koreans were lower than those of Japanese in 1988. The intake of lipid by Koreans was less than one half of the intake by Japanese. The intake of protein has been increased to 79.2g in Korea and 91.6g in Japan, of which 49% and 52.6% were provided by the animal sources in Korea and Japan, respectively, in 1988. In terms of consumption of the energy nutrients, the percentage of carbohydrate has been decreased, while those of protein and lipid have been increased in both countries. In 1988, the ratios of energy intakes by carbohydrate, protein and lipid were 67 : 19 : 14 in Korea and 59 : 15 : 26 in Japan. Considering the desired ratio, 65 : 15 : 20, the intake of carbohydrate seems to be still high and that of lipid still low for Koreans. The food supply has been increased in both countries and the supply of animal food, oil and fat were greater in Japan than in Korea.

• 한국약용작물학회:학술대회논문집
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• 2008.11a
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• pp.161-162
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• 2008

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2006.11a
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• pp.521-524
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• 2006

본 연구에서는 농업폐기물인 감귤박의 효율적인 재활용 방안으로 고부가가치 제품인 활성탄을 제조하여 이용하는 가능성에 대하여 조사한 결과, 감귤박 폐액으로부터 건조한 시료의 조성은 대부분이 탄수화물로 구성되어 있으며, 또한 이는 미생물, 농약 및 중금속에 오염되지 않은 순수한 천연 재료로 구성되어 있음을 알 수 있었다. 또한 탄화공정 및 활성화공정을 통해 제조된 활성탄의 요오드 흡착능이 KS 규격 중 1급(활성탄 1,100 mg/g 이상)보다 높은 값으로 나타나 공업용 활성탄으로의 활용이 가능할 것으로 사료된다.

• Proceedings of the Korean Society of Crop Science Conference
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• 2002.10a
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• pp.56-56
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• 2002