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The Effect of Chlorhexidine on Early Healing Stage of Guided Tissue Regeneration (조직유도재생술의 초기치유에 있어서 클로르헥시딘의 영향)

  • Lee, Jung-Yeon;Han, Soo-Boo;Um, Heung-Sik
    • Journal of Periodontal and Implant Science
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    • v.27 no.4
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    • pp.723-737
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    • 1997
  • 본 연구의 목적은 조직유도재생술의 초기치유시에 구강양치액으로 사용되어지는 0.1% 클로르헥시딘과 0.2% 클로르헥시딘을 사용했을 경우, 양치액을 사용하지 않았을 경우의 세균감염 정도를 비교하는 것이다. 30명의 성인형 치주염에 이환되어진 사람을 대상으로 하였다. 초기치료(Scaling/Root planing/Oral hygiene instruction)를 시행한 후에 한 사람에 한 군데씩 선정하여 2급이나 3급의 치근이개부를 가지고 임상적으로 혹은 방사선학적으로 치간골내낭을 보이지 않는 치아에 통법에 따라 Gore-TexTM를 위치시켰다. 술후 5일간 항생제 (UnasynTM 375mg tablet p.o.tid)를 투여하고 차폐막을 제거할 때까지(4주 혹은 6주) 10명의 환자에게는 0.1% 클로르헥시딘을, 다른 10명의 환자에게는 0.2% 클로르헥시딘으로 구강양치를 하게 하고, 또 다른 10명의 환자에게는 구강양치액을 사용하지 않도록 하였다. 또 1주일에 한번씩 전문가구강위생술식을 실시하였다. 4주나 6주 후에 차폐막을 제거하고 주사전자현미경, 혐기성 세균배양을 이용하여 세균감염정도를 비교하였다. 1. 주사전자현미경으로 관찰시에 0.1% 클로르헥시딘을 사용했을 경우와 0.2% 클로르헥시딘을 사용했을 경우, 클로르헥시딘을 사용하지 않은 경우에 별 차이를 발견할 수 없었다. 2. 혐기성 세균배양시에 0.2% 클로르헥시딘을 사용했을 경우, 0.1%클로르헥시딘을 사용했을 경우보다 적은 수의 세균 수를 보였으나 통계적으로 유의할 만한 차이는 보이지 않았다. 클로르헥시딘을 사용하지 않은 경우에는 다른 두 경우에 비해 통계적으로 유의할 만한 차이를 보였다.(P<0.05) 3. Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia를 인지한 경우에는 세 경우 모두 비슷한 비율로 발견되었다.

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$TiO_2$/Carbon felt의 광전기 화학반응에 의한 퍼클로레이트 이온 제거

  • Kim, Jong-U;Min, Hyeong-Seop;Ju, Byeong-Gwon;Lee, Jeon-Guk
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2009.05a
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    • pp.57.2-57.2
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    • 2009
  • 퍼클로레이트 이온($ClO_4^-$)는 자연적으로 혹은 인공적으로 만들어지며 퍼클로릭산이나 암모늄 퍼클로레이트나, 포타슘 퍼클로레이트 혹은 소듐퍼클로레이트 염의 형태로 존재하며, 물에 아주 잘 녹고, 끓여도 제거되지 않으며, 활성 탄소와 같은 광물에도 흡착 되지 않는 성질로 인해, 기존 물리적인 정수 방법으로는 제거하기 어렵다. 또한 우리 몸에 흡수되면, 요오드가 갑상선에 흡수되는 작용을 방해하여 갑상선 기능장애를 초래한다. 이러한 퍼클로레이트 이온의 제거방법으로는 이온교환법이나 생물학적 방법 등이 개발되어져 있으나, 제거 시스템에 이동 및 안전한 농도까지 제거 등의 문제점으로 인한 퍼클로레이트 이온을 환원시키는 촉매 환원 반응에 의한 퍼클로레이트 이온 제거 기술 개발이 필요하다. 이런 촉매 환원에 필요한 수소 환원제를 발생시키기 위해서, 본 연구에서는 Carbon felt 위에 DC magnetron sputtering에 의한 thin film $TiO_2$과 regine을 이용한 powder $TiO_2$ 시편을 제작하였다. 이렇게 제작 된 $TiO_2$/Carbon felt의 미세구조 및 특성은 XRD, SEM, UV-vis-NIR 등을 통하여 분석하였다. UV 조사에 의해 $TiO_2$/Carbon felt 시편의 산소와 수소 발생과 DC bias의 걸어주었을 때 산소와 수소 발생 차이 등을 비교하였고, 이에 따른 퍼클로 레이트 이온의 분해 영향을 알아보았다.

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Biological Perchlorate Reduction in Municipal Sewage (도시하수에서 생물학적 퍼클로레이트의 환원)

  • Choi, Hyeoksun
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.9
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    • pp.675-680
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    • 2013
  • This research was done to evaluate the potential for destruction of perchlorate in municipal sewage. Laboratory experiments were conducted in flasks containing 3 liters of raw sewage. Sewage was mixed with defined amount of perchlorate and various additives. Perchlorate reduction in sewage did occur, but was quite variable, ranging from 0 to 72% over 72 hour. Addition of even a small amount of perchlorate acclimated biomass (167 mg/L SS) significantly reduced the lag and resulted in complete perchlorate removal. Perchlorate reduction in sewage-brine mixtures was inhibited when the dissolved oxygen level was greater than 2 mg/L, and when the mixture salinity was relatively high (conductivity = 14 mS with equivalent TDS = 8 g/L). When nitrate ($NO_3{^-}$) was present with perchlorate in the laboratory flask tests of sewage-brine mixtures, nitrate reduction proceeded first. A significant amount of nitrite ($NO_2{^-}$) accumulated in the sewage-brine mixtures, accounting for about 66% of initial nitrate nitrogen ($NO_3$-N).

Biological reduction of perchlorate containing high salinity (고농도 염을 포함한 퍼클로레이트의 생물학적 환원)

  • Hwang, Jungwon;Park, Doori;Lee, Kanghoon;Yeom, Icktae
    • Proceedings of the Korea Water Resources Association Conference
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    • 2015.05a
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    • pp.284-284
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    • 2015
  • 본 연구는 퍼클로레이트의 생물학적 환원 과정에 있어서 고농도의 염이 미생물에게 어떤 영향을 미치는지를 다양한 방법을 통해서 알아보고 적절한 모델링 접근을 통하여 최적 환원속도를 위한 반응조 조건 및 설계에 필요한 요소들을 도출하기 위해 수행되었다. 100mL 합성폐수를 포함하는 플라스크를 이용한 실험이 수행되었고, 일정 농도의 퍼클로레이트와 유일 탄소원으로 아세트산나트륨이 사용되었다. 염화나트륨 농도가 $7490{\mu}s/cm$에서 $23700{\mu}s/cm$까지 증가하는 동안 퍼클로레이트의 생물학적 환원 속도는 현저하게 감소하였으며, $32100{\mu}s/cm$ 이상의 염화나트륨 농도에서는 퍼클로레이트가 환원되지 않았다. 동일한 농도의 염화나트륨, 염화암모늄, 염산 및 황산이 포함된 하수에서는 퍼클로레이트의 환원속도가 모두 비슷하였다.

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Spectrophotometric Assay for Determination of Chlorogenic Acid Using Green Pigment Formation and Quantitative Analysis of Chlorogenic Acid in Blueberry Leaf (녹색반응을 이용한 클로로겐산의 함량측정을 위한 흡광도 분석법과 블루베리 잎에 함유된 클로로 겐산의 함량분석)

  • Chung, Dong-Min;Chung, Young-Chul;Chun, Hyo-Kon
    • Journal of Life Science
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    • v.21 no.4
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    • pp.610-612
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    • 2011
  • We developed a spectrophotometric assay for the quantitative determination of chlorogenic acid based on the formation of green pigment at $50^{\circ}C$ under glycine and alkaline conditions in 96-well plates. The formation of green pigment was linear with a series of chlorogenic acid concentration (0-$300\;{\mu}M$). Using this method, the content of chlorogenic acid (12.42 mg/g dry weight) in the leaves of blueberry was quantified. This method is high-throughput, cost-effective, rapid, and easy to perform.

Monitoring and Evaluation of Analytical Methods of Perchlorate with IC and LC/MS (IC와 LC-MS를 이용한 퍼클로레이트 분석 방법 비교 및 모니터링)

  • Kim, Hwa-Bin;Sim, Won-Jin;Kim, Min-Young;Oh, Jeong-Eun
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.1
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    • pp.37-44
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    • 2008
  • In this study, we tried to establish analysis methods of perchlorate with ion chromatography(IC) and liquid chromatography/mass spectrometry(LC/MS) and monitored perchlorate levels in various kinds of water and soil samples. The obtained method detection limit(MDL) of IC was 1 ppb and that of LC/MS was 0.005 ppb in water sample. We monitored the ground and spring water in Busan and the average perchlorate level in ground water was 0.031 $\pm$ 0.011 ppb and that of spring water was 0.013 $\pm$ 0.014 ppb. Wastewater samples were also examined and the levels of perchlorate ranged from 0.007 to 0.380 ppb. The perchlorate levels in all water samples investigated in this study were below the EPA guideline.

Inhibition of Biological Perchlorate Reduction by Nitrate and Oxygen (질산염과 산소에 의한 생물학적 퍼클로레이트 환원의 저해)

  • Choi, Hyeok-Sun
    • Journal of Soil and Groundwater Environment
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    • v.14 no.6
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    • pp.29-34
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    • 2009
  • In this study, a chloride ion probe as a direct measurement for perchlorate reduction was used to determine whether biological perchlorate reduction was inhibited by other electron acceptors ($O_2$ and ${NO_3}^-$) and to investigate competition of electron acceptors for using electron donors. Profiles of chloride production (= perchlorate reduction) in flasks containing perchlorate reducing populations were monitored by a chloride ion probe. Biological reduction of 2 mM perchlorate was inhibited by 2 mM nitrate that chloride production rate was decreased by 30% compared to perchlorate used as the only electron acceptor and chloride production rate was decreased by 70% when acetate was limited. Reduction of 2mM perchlorate was completely inhibited by oxygen at 7~8 mg/L, regardless of acetate excess / limitation.

Biological reduction of perchlorate containing high salinity (퍼클로레이트의 생물학적 환원에 나이트레이트가 주는 영향)

  • Jun, Moonhwee;Hwang, Jungwon;Lee, Jihee;Lee, Kanghoon;Yeom, Icktae
    • Proceedings of the Korea Water Resources Association Conference
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    • 2015.05a
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    • pp.277-277
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    • 2015
  • 본 연구는 퍼클로레이트의 생물학적 환원 과정에 있어서 나이트레이트의 존재가 미생물에게 어떤 영향을 미치는지를 실험을 통해서 알아보고 적절한 모델링 접근을 통하여 나이트레이트의 퍼클로레이트 환원에 대한 저해의 정량적 분석을 위한 요소들을 도출하기 위해 수행되었다. 100mL 합성폐수를 포함하는 플라스크를 이용한 실험이 수행되었고, 유일 탄소원으로 아세트산나트륨이 사용되었고, 전자수용체로는 퍼클로레이트와 나이트레이트가 사용되었다. 먼저 퍼클로레이트와 나이트레이트 각각을 단일전자수용체로서 넣은 실험을 진행하였다. 퍼클로레이트의 동역학계수 qmax, Ks, Y, b값은 각각 0.9(mgClO4-/mgMLSSday), 42.28(mgClO4-/L), 0.382(mgClO4-/mgMLSS), 0.05(day-1)로 계산되었다. 그리고 나이트레이트의 동역학 계수 qmax, Ks, Y, b값은 각각 13.81(mgNO3-/mgMLSSday), 239.78(mgNO3-/L), 0.275(mgNO3-/mgMLSS), 0.05(day-1)로 계산되었다. 나이트레이트와 퍼클로레이트를 동시에 넣었을 경우에는 나이트레이트의 동역학 계수는 qmax, Ks, Y, b 값은 각각 13.72(mgClO4-/mgMLSSday), 235.64(mgClO4-/L), 0.263(mgClO4-/mgMLSS), 0.05(day-1)로 큰차이 없었으나, 퍼클로레이트의 경우에는 qmax, Ks, Y, b값은 각각 0.6(mgClO4-/ mgMLSSday), 42.24(mgClO4-/L), 0.393(mgClO4-/mgMLSS), 0.05(day-1)로 qmax값은 감소하였고, Y값은 증가하는 모습을 보임으로써, 나이트레이트의 존재가 퍼클로레이트의 환원을 저해시키는 것을 확인할 수 있었다.

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The effects of aqueous extracts of plant roots on germination of seeds and growth of seedings (식물근의 추출물질이 종자발아 및 유식물의 생장에 미치는 영향)

  • Chan-Ho Park
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.4 no.1
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    • pp.1-23
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    • 1968
  • This study aimed at contributing to the improvement of cropping systems after finding out the effects of excrements and components of crop root influence on other crops as well as themselves. The following forage crops suitable for our country were selected for the present study. Aqueous extracts of fresh roots, aqueous extracts of rotting roots and aqueous solutions of excrements of red clover, orchard grass and brome grass were studied for the effects influencing the germination and growth of seedlings of red clover, ladino clover, lespedeza, soybean, orchard grass, Italian ryegrass, brome grass, barley, wheat, sorghum, corn and Hog-millet. In view of the possibility that the organic acid might be closely related to the excrements and components of crop root connected with soil sickness, the acid components of three species of roots were analysed by paper chromatography and gas chromatography method. The following results were obtained: 1. Effects of Aqueous Extracts of Fresh Roots : Aqueous extracts of red clover: The extracts inhibited the growth of seedlings of the ladino clover and lespedeza and also inhibited the development of most crops except that of sorghum among the Graminaceae. Aqueous extracts of orchard grass: The extracts promoted the seedlings growth of red clover and soybean, while it inhibited the germination and growth of orchard grass. There were no noticeable effects influencing other crops while it inhibited the growth of barley and Hog-millet. Aqueous extracts of brome grass: There was no effect on Italian ryegrass but there was an inhibiting effect on the other crops. 2. Effects of Aqueous Extracts of Rotting Roots : Aqueous extracts of red clover: The extracts promoted the seedling growth of red clover. But it reflected the inhibiting effects on other crops except sorghum. Aqueous extracts of orchard grass: The extracts promoted the growth of red clover, ladino clover, soybean and sorghun, while it inhibited the germination and rooting of barley and Hog-millet. Aqueous extracts of brome grass: The extracts gave the promotive effects to the growth of red clover, soybean and sorghum, but caused inhibiting effects on orchard grass, brome grass, barley and Hog-millet. 3. Effects of Aqueous Solutions of Excrements : The aqueous solution of excrements of red clover reflected the inhibition effects to the growth of Graminaceae, while the aqueous solutions of excrements of orchard grass and Italian ryegrass caused the promotive effects on the growth of red clover. 4. Results of Organic Acid Analysis : The oxalic acid, citric acid, tartaric acid, malonic acid, malic acid and succinic acid were included in the roots of red clover as unvolatile organic acid, and in the orchard grass and brome grass there were included the oxalic acid, citric acid, tartaric acid and malic acid. And formic acid was confirmed in the red clover, orchard grass and brome grass as volatile organic acid. In consideration of the results mentioned in above the effects of excrements and components of roots found in this studies may be summarized as follows. 1) The red clover generally gave a disadvantageous effect on the Graminaceae. Such trend was considered chiefly caused by the presence of many organic acids, namely oxalic, citric, tartaric, malonic, malic, succinic and formic acid. 2) The orchard grass generally gave an advantageous effect on the Leguminosae. This may be due to a few kinds of organic acid contained in the root, namely oxalic, citric, tartaric, malic and formic acid. Furthermore a certain of promotive materials for growth was noted. 3) As long as the root of brome grass are not rotten, it gave a disadvantageous effect on the Leguminosae and Graminaceae. This may be due to the fact that several unidentified volatile organic acid were also included besides the confirmed organic acid, namely oxalic, citric, tartaric, malic and formic acid. 5. Effects of Components in Roots to the Soil Sickness : 1) It was considered that the cause of alleged red clover's soil sickness did not result from the toxic components of the roots. 2) It was recognized that the toxic components of roots might be the cause of soil sickness in case the orchard grass and brome grass were put into the long-term single cropping. 6. Effects of Rooted Components to the Companion Crops in the Cropping System : a) In case of aqueous extracts of fresh roots and aqueous excrements (Inter cropping and mixed cropping) : 1) Advantageous combinations : Orchard grass->Red clover, Soybean, Italian ryegrass->Red clover, 2) Disadvantageous combinations : Red clover->Ladino clover, Lespedeza, Orchard grass, Italian ryegrass, Fescue Ky-31, Brome grass, Barley, Wheat, Corn and Hog.millet, Orchard grass->Lespedeza, Orchard grass, Barley and Hog-millet, Brome grass->Red clover, Ladino clover, Lespedeza, Soybean, Orchard grass, Brome grass, Barley, Wheat, Sorghum, Corn and Hog-millet, 3) Harmless combinations : Red clover->Red clover, Soybean and Sorghum, Orchard grass->Ladino clover, Italian ryegrass, Brome grass, Wheat, Sorghum and Corn, Brome grass->Italian ryegrass, b) In case of aquecus extracts of rotting roots(After cropping) : 1) Advantageous combinations : Red clover->Red clover and Sorghum, Orchard grass->Red clover, Ladino clover, Soybean, Sorghum, and Corn, Brome grass->Red clover, Soybean and Sorghum, 2) Disadvantageous combinations : Red clover->Lespedeza, Orchard grass, Italian ryegrass, Brome grass, Barley, Wheat, and Hog-millet Orchard grass->Barley and Hog-millet, Brome grass->Orchard grass, Brome grass, Barley and Hog-millet, 3) Harmless combinations : Red clover->Ladino clover, Soybean and Corn, Orchard grass->Lespedeza, Orchard grass, Italian ryegrass, Brome grass and Wheat Brome gass->Ladino clover, Lespedeza, Italian ryegrass and Wheat.

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The Hydrolysis Measurement of Cyclodextrins Using FTIR-ATR Spectrometry (FTIR-ATR 분광법을 이용한 사이클로덱스트린의 가수분해 측정)

  • Chung, Chinkap
    • Analytical Science and Technology
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    • v.13 no.5
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    • pp.549-557
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    • 2000
  • FRIR-ATR spectrometry has been used to monitor the aqueous reactions of compounds without distinct chromophores in ultraviolet and visible regions. For example, hydrolysis reactions of ${\alpha}$-cyclodextrin and ${\gamma}$-cyclodextrin in acidic aqueous solution were studied. FTIR-ATR method has been used for the monitoring of cyclodextrin hydrolysis in 1.0 M. 0.5 M, and 0.1 M HCl solutions, respectively. We also found that the hydrolysis of ${\alpha}$-cyclodextrin produced glucose, but the hydrolysis of ${\gamma}$-cyclodextrin proceeded further to give more fragmented products than glucose.

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