• Korean Journal of Microbiology
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• v.40 no.2
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• pp.100-103
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• 2004

The culture condition and medium composition for the enhanced mycelial growth of Inonotus mikadoi IMSNU 32058 were investigated. The optimal temperature and pH for the mycelial growth were $27^{\circ}C$ and pH 4.5, respectively. Among the complex media tested, the malt extract medium and Phellinus igniarius medium were very good for mycelial growth of I. mikadoi. When Czapek-Dox medium was used as a minimal medium for cultivation of mycelia, xylose, raffinose and carboxymethyl cellulose were very excellent as a carbon and energy source. With respect to carbohydrate, sucrose and glucose were very good carbon sources. In general, organic complex nitrogen sources were better than other inorganic ones. As the organic complex nitrogen sources tested, yeast extract, soytone, proteose peptone and bacto peptone were the best as a source of organic nitrogen. When ammonium sulfate as an inorganic source of nitrogen was used, the enhanced mycelial growth was shown. p-Aminobenzoic acid was proved to be most appropriate source of vitamin. After the mycelia of I. mikadoi was cultivated at $27^{\circ}C$ for 5 days in MEM broth (pH 4.5), the activities of both exomycelial and endo-mycelial enzymes were determined. Among endomycelial enzymes assayed, the specific activity of laccase was much higher than those of other enzymes. When the fungus was grown in MEM broth, exomycelial specific enzyme activity of laccase was comparatively high. However, little or no enzyme activities of protease, chitinase and lipase were found.

• Applied Biological Chemistry
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• v.27 no.4
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• pp.217-224
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• 1984

Effect of a thermophilic yeast (strain T-71) on the ethanol fermentation of raw starchy materials was investigated. The maximum temperature of the thermophilic yeast for the growing and fermentation was a little higher than that of ordinary yeasts and their resistance to ethanol was also high. Even though the optimum temperature of the thermophilic yeast for fermenting ethanol of several raw starchy materials was different depending upon the concentration of mashing, their optimum fermentation temperature was higher than the ordinary yeasts in all cases studied, and their fermentation efficiency was good enough to use. It was also found from the study that the period of fermentation could be shortened for about one to two days by using the thermophilic yeast.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 1998.06a
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• pp.12-16
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• 1998

최적 위치별 퇴적토에 대한 토양 특성 및 수목 식재용 유기토양 활용 가능성을 알아보고 각 시료의 수분, 유기물 함량 등에 따른 적절한 혼합을 통하여 식물 발아 성장의 최적화를 온실에서의 식물재배 실험을 통하여 달성하고자 한다. 또한 식재용 토양 활용 가능성을 알아보기 위한 기본 물성 및 유해성 평가를 하여 중금속이 식물 성장에 어떤 영향을 미치는가를 고려하여 재활용 여부를 알아보았다. 퇴적토를 이용한 식물의 발아 및 생장 실험은 원퇴적토와 모래와의 혼합비를 달리하고 다른 불순물이 함유되지 않도록 하여 발아일수와 발아적온, 생육적온 등 최적 조건을 팬지와 금잔화에 대하여 실험하였다.

• Korean Journal of Microbiology
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• v.38 no.1
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• pp.50-53
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• 2002

The optimum temperature and pH for mycelial growth of B. bassiana DGUM 34001 were $24^{\circ}C$ and pH 7.0, respectively. Among the complex media used, mushroom complex medium (MCM) was the most favorable for mycelial growth. When Czapek-Dox medium was used as a minimal medium, glucose was an excellent source for carbon and energy. Soytone and sodium phosphate were favorable constituent for culture medium as a source of organic nitrogen and phosphorus, respectively. When the fungus was grown in MCM broth, the specific activity of extracellular enzyme of ${\alpha}$-amylase, lipase, chitinase, CMCase and pretease were 297.0, 0.058, 0.33, 0.21 and 22.8 units/mg protein, respectively. When various sources of organic nitrogen and chitin were supplemented to determine the production of enzymes, casein and soluble chitosan enhanced the production of extracellular protease and chitinase.

• Microbiology and Biotechnology Letters
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• v.12 no.4
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• pp.271-276
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• 1984

Effect of C/N ratios on the cell growth, cell lipid, cell protein and fatty acids of Rhodotorula sp. was examined. At low concentration of nitrogen in cultural medium the growth of the yeast was retarded, whereas its growth was favorable at high concentration but lipid yield was decreased due to a high content of cell protein. In order to increase lipid yield, the optimum C/N ratio obtained was 45-50 (5% glucose, 0.0414% nitrogen). During the cultivation cell protein synthesis occurred mainly in initia stage and the lipid was accumulated rapidly at the late phase. The highest content of total fatty acid was observed at the optimum C/N ratio. The higher C/N ratio was the more unsaturation degree of fatty acid decreased. It was attributed principally to a decreased oleic acid with palmitic acid increased. No significant changes occurred in content of other fatty acids.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.88-92
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• 2006

Nerine was originated from South Africa and around 30 kinds of species are distributed in worldwide. In present study we have characterized the division methods and growing conditions for the bulb propagation of Nerine. Result indicated that new bulb formation, and growth of N. bowdenii cultivars was better after dormancy stage compared to post-harvest and dormancy stages, under twin-scale method at $20^{\circ}C$ Similarly, new bulb tissue density was higher at $20^{\circ}C$ compared to other temperatures. Moreover, N. bowdenii 'Favourite' exhibited better growth than N. bowdenii 'Stepanii' and vermiculite was found to be most suitable growing medium for Nerine bulb propagation.

• Korean Journal of Agricultural Science
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• v.11 no.1
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• pp.53-60
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• 1984

The followings are the results obtained from the investigation of isolation and cultural conditions of a fungus producing antibiotic from humus, spring soil, paddy, field and etc. 1. Among fungi which were capable of antimicrobial sub stance producing at the $45^{\circ}C$, Strain H-3 was selected as test st rain because of its efficiency in antimicrobial substance producing. 2. Strain H-3 was identified as a Aspergullus fumigatus by the mophological properties. 3. The compositions of optimun media for the production of antimicrobial substance were followed;glucose 20g, $NaNO_3$ 3g, $K_2HPO_4$ 1g, $CaCl_2$ 0.5g, $MgSo_4$ 0.5g, $FeSO_4$ 0.005g. 4. Optimum culture conditions were founded to be pH 5.0, $40^{\circ}C$ respectively. 5. The maximum antimicrobial substance producing obtained in this study was showed 14mm of clear zone after 72 hrs incubation under the optimum conditions. 6. AF-c substance was active against Gram-positive, Gram-negative bacteria, and yeast but not mold in its biological properties.

• Korean Journal of Plant Resources
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• v.29 no.4
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• pp.393-399
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• 2016

This study was conducted to elucidate the photosynthetic response to the environment and establish optimum cultivation conditions for the Korean endemic plant, Aster koraiensis. Photosynthetic characteristics according to growth stage, light, CO₂, and soil water potential were investigated. During the first year of transplanting, photosynthetic rates were drastically increased until June, after which they slowly declined, During the second year, photosynthetic rates declined throughout the entire growth period. The highest level of light compensation point was shown the early growth stage. Photosynthetic rates affected by intercellular CO₂ concentration were maintained or decreased over the CO₂ saturation point. The lowest CO₂ compensation point was 16.1 μmol·mol⁻¹ during March. The morphological changes of leaves were observed due to shading with chlorophyll contents increasing. Photosynthetic rates were higher at 0% and 50% shading treatments than at 75%. There were rarely any morphological changes of leaves due to soil moisture, however, changes to leaf compactness were observed. Photosynthetic rate, apparent quantum yield, and respiration rate increased, whereas water use efficiency decreased over −25 kPa of soil moisture.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.23-23
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• 2020

털부처꽃(Lythrum salicaria L.)은 예로부터 발효해서 술로 마시고, 잎은 채소로 식용하며, 식중독 치료 및 설사를 멈추게 하는 효과가 있는 것으로 알려져 있다. 한국, 중국, 아프리카, 유럽, 북아메리카 등의 습한 지역에 분포하며, 7~8월에 취산꽃차례로 붉은 자주색의 꽃이 핀다. 본 연구는 털부처꽃을 지피용 소재로 개발하기 위한 최적의 재배기술을 확립하기 위해 수행되었다. 재배방법의 확립을 위하여 2019년 4월 27일에 파종하여 생산된 유묘를 7월 4일에 정식하여 9월 24일까지 12주 동안 재배하였다. 공통 조건은 3치 비닐 포트에 원예상토를 충진하고, 200구 트레이에 셀당 1립씩 파종하여 생산된 1셀 묘를 정식하였으며, 추비, 차광, 적심 등은 처리하지 않았다. 추비 실험은 hyponex 하이그레이드(N-P-K, 7-10-6)를 0, 500, 1000, 2000mg·L^-1를 4주간 간격으로 총 3회 엽면시비 하였다. 차광 정도 실험은 0, 35, 55, 75% 차광막을 이용하였으며, 적심은 무적심과 4주차에 1회 적심처리 하였다. 연구의 결과, 털부처꽃은 추비 농도가 증가할수록, 생육이 감소하는 결과를 보였다. 차광처리 별로는 무차광 조건에서 생육이 가장 왕성한 결과를 보였고, 광량의 부족은 전반적으로 생육을 억제하는 경향이었다. 적심 처리는 줄기 신장을 크게 억제하였으며, 측지수를 증가시키는 결과를 보였다. 엽수는 무처리와 비교하였을 때 유의적인 차이가 없는 것으로 확인되었다. 따라서 털부처꽃은 적심을 하는 것이 효과적인 것을 알 수 있었으며, 적심 시기에 관한 추가적인 연구가 필요할 것으로 생각된다. 결론적으로 추비처리는 털부처꽃의 생육에 부정적인 영향을 주므로 처리하지 않고, 무차광 조건에서 재배하는 것이 전반적인 생육에 유리하였다. 또한 도장이 잘되는 식물임으로 적심처리하는 것이 좋을 것으로 판단된다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.49-49
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• 2018

본 연구는 관상가치가 높아 조경 및 관상소재로 개발이 가능한 큰개관중의 전엽체 증식 및 포자체 형성에 적합한 배양조건을 구명하고자 수행되었다. 실험재료는 무가온 온실에서 채집한 포자를 기내에서 발아시켜 전엽체를 획득한 후 8주 간격으로 계대배양하여 실험에 사용하였다. 배지종류에 따른 전엽체의 기내 증식 및 형태형성의 영향을 알아보고자 배양된 전엽체 300mg을 메스로 다진 다음 농도를 1/4, 1/2, 1, 2배로 조절한 MS와 Knop배지에 8주간 배양하였다. 그 결과, 1MS배지에서 전엽체의 생체중이 5.5g으로 가장 많이 증가하였다. 1MS를 제외한 타 처리구는 생체중의 증가수준이 1.1-3.0g에 머물러 1MS배지보다 저조한 수준을 보였다. 현미경을 이용한 전엽체의 관찰결과, 1MS배지는 엽육의 색이 녹색으로 생육이 양호하였다. 전엽체의 증식이 가장 저조하였던, 2MS와 1/4MS는 생육의 저조뿐만 아니라 노화현상도 관찰되었다. 포자체 형성을 위한 최적의 토양조건을 알아보고자, 원예상토, 피트모스, 펄라이트 및 마사토의 비율을 5종류로 달리하여 배양토를 혼합하였다. 혼합된 토양은 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진하여 기내배양된 전엽체 1g을 증류수와 함께 10초간 분쇄한 다음 토양표면에 분주 후 10주간 재배하였다. 그 결과, 원예상토가 높은 비율로 첨가된 혼합조건에서 포자체의 형성이 우수하였다. 그중 원예상토와 마사토를 2:1(v:v)로 혼합한 토양, 원예상토와 펄라이트를 2:1(v:v)로 혼합한 토양에서 포트 당 각 357.0, 339.8개의 포자체가 형성되었다. 또한 형성된 포자체의 생육을 조사한 결과 원예상토와 마사토를 2:1(v:v)로 혼합한 토양에서 생체중, 엽수, 엽장, 엽폭, 근수, 근장 및 SPAD value 등의 생육수치가 우수하였다. 따라서 큰개관중의 전엽체 증식에 적합한 배지는 1MS로 판단되었으며, 포자체 대량생산을 위해서는 원예상토와 마사토를 2:1(v:v)로 혼합한 토양이 적합하다고 판단된다.