• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2005년도 정기총회 및 제35차 춘계 학술 발표대회
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• pp.199-202
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• 2005

본 연구는 cytochrome B 유전자의 PCR-RFLP 분석기법을 이용하여 다양한 가금류 식육자원 및 각종 가공 육제품의 원료육에 대한 정확하고 재현성 높은 축종 및 육종 감별기술을 개발하기 위하여 수행되었다. 국내에서 유통되고 있는 종간 근연관계가 높은 3종의 가금육(닭, 칠면조 및 오리)의 육류에서 분리한 DNA 시료를 대상으로 cytochrome B 유전자의 특정 염기서열을 포함하는 primer를 설계 제작하여 PCR-RFLP 분석을 실시하였다. 각 축종의 근육조직으로부터 genomic DNA를 추출하고 PCR 증폭 반응을 수행한 후 얻어진 PCR 증폭산물(359 bp)을 두 종류의 제한효소(HaeШ 및 Hinf I)로 각각 절단한 결과 특히, HaeШ 제한효소에서 가금류의 축종 간 그리고 제한효소 간에 명확한 차이를 보이는 종 특이적인 PCR-RFLP marker를 검출하였다. 따라서, 본 연구에서 개발한 cytochrome B 유전자의 가금류 종 특이적 PCR-RFLP marker는 가금류의 식육 및 가공 육제품의 육종 및 축종 판별에 매우 유용한 DNA marker로 이용될 수 있을 것이다.

• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2005년도 제36차 추계 학술발표대회
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• pp.123-126
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• 2005

본 연구는 고등동물의 불포화지방산 생합성의 핵심 효소로 알려져 있는 Stearoyl-CoA desaturase(SCD) 유전자의 특정한 단일염기다형(single nucleotide polymorphism; SNP)이 한우의 도체 및 육질형질에 미치는 영향을 분석하기 위해 SCD 유전자의 Intron 7번 영역의 특정부위를 포함하는 primer를 제작하고, 염기서열 분석을 통하여 유전자 구조를 해석한 결과 총 211bp 크기를 갖는 염기서열의 122번째에서 아데닌(A)${\leftrightarrow}$구아닌(G) 염기치환으로 발생한 단일염기다형(SNP) 부위를 발견하였다. 이들 단일염기다형 염기서열 부위를 PCR-SSCP(single-strand conformation polymorphism) 기법을 이용하여 분석한 결과 3종류의 SNP 유전자형(A/A, A/G 및 G/G)을 검출하였다. 이 가운데 A/G 유전자형이 한우의 근내지방도와 등지방두께와 고도의 유의적 연관성이 있다는 새로운 사실을 발견하였다. 따라서, 본 연구를 통해 개발된 한우 SCD 유전자의 특정한 단일 염기다형 표지인자는 한우의 연령 및 성별에 관계없이 육질이 우수한 고급육을 생산하는 우량 한우의 조기식별에 매우 유용한 DNA 표지인자로 활용할 수 있을 것으로 기대된다.

• 대한안전경영과학회:학술대회논문집
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• 대한안전경영과학회 2013년 추계학술대회
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• pp.561-567
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• 2013

본 연구는 졸음운전의 지표로 예상되는 심전도의 LF/HF 비율이 효과적으로 졸음운전을 예측하는지를 검증하는 것이었다. 본 연구는 총 31명이 참가하였으며, 가상 운전 시뮬레이션 과제를 활용하여 진행하였다. 수면박탈이 운전 중 LF/HF 비율에 영향을 미치는지를 검증하기 위해 충분한 수면을 취한 조건과 수면이 박탈된 조건으로 실험을 실시하였다. 충분한 수면을 취한 조건에서 참가자는 전날 6시간 이상의 수면을 취한 후 30분동안 진행되는 가상 운전과제를 수행하였다. 수면이 박탈된 조건에서는 실험에 참여하기 전날에 참가자가 5시간 이하의 수면을 취하도록 유도한 후 60분 동안 진행되는 가상 운전 과제에 참여하도록 하였다. 참가자는 두 조건 모두에서 심전도를 측정할 수 있는 장비를 착용한 상태로 가상 운전 과제를 수행하였다. LF/HF 비율과 지각된 졸음운전과의 관계성을 확인하기 위해서 참가자가 가상 운전 과제를 수행하는 동안 10분간격으로 주관적 졸림정도를 측정하였다. 실험 결과 충분한 수면을 취한 조건보다 수면박탈 조건에서 참가자의 LF/HF 비율이 감소하였으며, 동일하게 주관적 졸림정도는 증가하였다. 또한 주관적 졸림정도가 LF/HF비율을 예측하는 것으로 나타났다. 따라서 LF/HF 비율을 통한 졸음 운전 예측은 타당한 것으로 나타났다.

• 사상체질의학회지
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• 제22권4호
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• pp.40-48
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• 2010

1. Objectives Herbal Extracts are used in Korean Oriental Medicine clinics. The sixty eight kinds of single herbal extracts and fifty six kinds of mixed herbal extracts has been using since the first year of execution of Korean medicine national insurance policy in 1987. This paper was performed to make a guideline of using herbal extracts. Single herbal extracts and mixed herbal extracts were discussed and classified according to Sasang constition. 2. Methods The sixty eight single herbal extracts were classified into four categories according to Sasang Constitution and fifty six mixed herbal extracts were classified by six Korean oriental medical doctors. Mixed herbal extracts were considered in terms of drug roles[sovereign, minister, assistant and courier(君臣佐使)] or most included ingredient herb. 3. Results Sasang constitutions which were suitable for fifty six mixed herbal extracts were Soeumin(46.4%), mixed constitution(39.2%) and Soyangin(8.9%), Taeeumin(5.3%). Sasang constitutions which were suitable for sixty eight single herbal extracts were Soeumin(47.1%), Soyangin(32.4%) and Taeeumin(20.6%). 4. Conclusions The rate of Soeumin herbal extracts was the most highest among all constitutions. It is regarded that warming and enforcing herb medicine were most used in all prescriptions.

• 복식문화연구
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• 제23권3호
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• pp.439-453
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• 2015

This research is regarding Park Ki-Jong's Western-style court costume and emphasizes the shape and style peculiarities of Western-style court costumes in the Korean Empire from the 1876 Port Opening to the 1910 annexation of Korean to Japan. Park Ki-Jong's frock coat was made during the period of the established law from 1900 to 1910. 1) The brand was ASADA TAILOR from Kyung-Sung. 2) The shape was long at the front and back, and it featured a picked lapel and double breast with six buttons to fasten and two buttons for decoration on the upper part. 3) The frock coat's materials were black wool fabric and black ridged silk. The lining's material was black plain silk and the sleeve's lining was white with blue striped silk. 4) The front separated the upper and bottom parts. The bottom was composed of a one-piece A-line skirt that continued from the front to back. The top of the back was separated by the princess line and the center-back seam was also separated with a vent. However, the center-back of the waistline was not separated, and it continued to one piece. The sleeve shape was a two-piece sleeve style with a phony vent and two wrapping buttons.

• 복식문화연구
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• 제25권3호
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• pp.340-358
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• 2017

The purpose of this study is to fundamentally examine the vests of the robes "Daeraebok" and "Soraebok" of the Korean Empire from the 1876 Port Opening to the 1910 annexation of Korea to Japan. Among the collections of different robes of the Korean Empire which belong to various universities and institutions, only the vests were surveyed. The shape of the vests in the Korean Empire were single-breasted and double-breasted. Most V-necklines and vests with a shawl collar look like a tailored collar, where the collar outline was shaped like a notched and picked collar, which is a mix of the step collar and roll collar of the 19th century. The rear center line was not flat, but inclined to a triangle. All vests of the robe Daeraebok were equipped with gold buttons, and those of the robe Soraebok had black satin buttons. The tailoring characteristic is that the front has a different material (dark black wool) from the back (black silk). This characteristic is expected to be an important basic piece of information in the restoration and reproduction of the vest, which was worn during the imperial period, especially to reveal its shape, characteristics and composition. This characteristic can also be used as data of cultural contents based on Korean modern history.

• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2004년도 정기총회 및 제33차 춘계 학술대회
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• pp.177-180
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• 2004

본 연구는 PCR-RFLP 및 PCR-SSCP 기법을 이용하여 PSE 돈육을 생산하는 PSS 돼지 유전자 진단 기술을 개발하고 이를 이용한 국내 종돈 및 교잡 비육돈의 PSS 유전자 출현 빈도를 파악하고자 수행하였다. 돼지 PSS의 원인이 되는 ryanodine receptor 유전자의 단일염기 돌연변이 $C{\rightarrow}T$ ; $Arg\;{\rightarrow}\;Cys$)를 포함하는 134 bp 영역을 PCR로 증폭한 후 RFLP 및 SSCP 기법으로 분석한 결과 동형접합체의 정상(N/N), 이형접합체의 잠재성 개체 (N/n) 그리고 돌연변이 유전자를 동형접합체 상태로 갖는 PSS 감수성 개체(n/n)에 각각 특이적인 유전자형이 검출되었다. 특히, PCR-SSCP기법을 이용한 RYR1 유전자 돌연변이 검출 방법은 보다 신속 간편하면서도 상대적으로 분석비용이 저렴한 정확성이 높은 PSS 돼지 진단기술로서 대규모 돼지집단검색이나 RFLP 방법으로 판정이 불확실한 시료의 재검에 효율적으로 이용할 수 있을 것으로 판단된다.

• Environmental Analysis Health and Toxicology
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• 제18권4호
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• pp.295-303
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• 2003

Physiological cellular activities responses to cadmium (Cd) exposure in green algae with several reductases activities and viability of the cell were examined. The cell division of green algae, Selenastrum capricornutum treated with 5ppm was significantly decreased than that of normal algae. The mean cell number of normal algal culture was as twice much as than that of algae at 6 days after Cd treatment. The cellular viability of algae was analysed by flow-cytometry with fluorescent dye after esterase reaction on cell membrane. The 85.35% of cellular viability of normal culture was decreased to 34.35% when algae was treated with 5 ppm of Cd at 6 days after treatment. It was considered that those method of flow-cytometry is useful tool for toxicity test on micro-organisms in the respect of identifying cellular viability. Also, the activities of both glutathione peroxidase (GPX) and ascorbate peroxidase (APX), which are indirectly react against oxidative stress through reduction of glutathione by Cd were significantly increased with 25%. It is considered that both GPX and APX are involved in the metabolic pathway of Cd -detoxification with similar portion in Selenasturm capricornutum.

• Environmental Analysis Health and Toxicology
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• 제18권4호
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• pp.305-310
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• 2003

A rapid, inexpensive enzymatic method is proposed for indirect water quality testing in terms of heavy metal toxicity. The activity of glucose-6-phosphate dehydrogenase was applied for heavy metal toxicity test as an effective criterion in water quality. The toxicity of Pb (lead) and Cd (cadmium) for water flea, Moina macrocopa, were evaluated for 2-8 days with variables of mobilization ability. And the reproduction impairment of Moina macrocopa were investigated as the parameter of chronic toxicity test for Pb and Cd. As a result, the EC$_{50}$ for immobilization of Moina macrocopa were Pb and Cd were 1.6749 and 0.4683, respectively. The values of reproductive impairment to Moina macrocopa for Pb and Cd were 9.5938 and 8.3264 in EC$_{50}$ A significant alteration of G6PDH (Glucose-6-phosphate dehydrogenase) activity of Moina macrocopa was observed when Cd and Pb were treated in media. The results obtained indicate that G6PDH activity of Moina macrocopa can be used as an indicative parameter in aquatic toxicity tests for heavy metals.als.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제22권1호
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• pp.51-55
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• 2000

Purpose : The purpose of this study is to setup a standard treatment protocol in surgical correction of skeletal malocclusion with Angle Class III patients. We asessed the soft tissue changes according to skeletal changes of patients who have undergone orthognathic surgery with bilateral split sagittal ramus osteotomy (BSSRO) and advancing genioplasty. Materials & Methods : The soft tissue change of 9 skeletal Class III patients was assessed after BSSRO and advancing genioplasty. The patient group was skeletal Class III who was surgically treated by BSSRO & advancing genioplasty. The average follow up period is 13 months with the range of 6 and 30 months. All patients have undergone preoperative and postoperative orthodontic treatment. The assessment was devided into two groups. One was antero-posterior relationship and the other was vertical relationship of dimensional changes of soft tissue after orthognathic surgery. Results : In antero-posterior dimensional changes after surgery, the percentage of soft tissue change in comparison to hard tissue was 89%. Vertical ratio after surgery, 86% soft tissue changes were assessed.