• Analytical Science and Technology
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• v.14 no.1
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• pp.64-71
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• 2001

In this paper, the formula of lower limit of detection considering the measuring time of sample and background was derived using the basic concepts of lower limit of detection. Among parameters affecting the determination of MDA value, the MDA values were calculated with variation of amount of sample and measuring time of sample and background. The results of adequate division between the measuring time of sample and that of background studied in this research make it possible to evaluate confidence limits on the radio analytical results in the environmental sample.

• Journal of the Korean Institute of Telematics and Electronics
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• v.27 no.10
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• pp.60-67
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• 1990

This paper proposes a model-based fault detection method for linear/nonlinear system having modelling errors, nonlinearities and measurement noise. The system model is represented by the unified operator [5] in order to apply to both the continuous-time and discrete-time problems. The fault detection method suggested here accounts for the effects of noise, model mismatch and nonlinearities. Modelling errors are depicted by additive forms and the nominal model denominator is fixed via prior experiments in order to quantify the nucertainty bound on the parameter estima-tion. The least square method is used to estimate the numerator parameters of the nominal model. performance than traditional methods.

• Korean Journal of Environmental Agriculture
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• v.22 no.3
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• pp.227-232
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• 2003

This study was conducted to evaluate the terminal residue of a new fungicide, KNF 1002, in cucumber and pepper under greenhouse and field conditions. When a microemulsion formulation (20%) of KNF 1002 was applied once or twice during $1{\sim}7$ days before harvest, its terminal residue in cucumber ranged <$0.02{\sim}0.20\;mg/kg$ under greenhouse condition. In pepper, its figure recorded $0.31{\sim}0.79\;mg/kg$ and $0.11{\sim}0.28\;mg/kg$ under greenhouse and field conditions, respectively. Much higher level of terminal residues was observed in leaves than those in fruits in pepper, showing $7.38{\sim}25.20\;mg/kg$ and $0.11{\sim}1.99\;mg/kg$ under greenhouse and field conditions, respectively. Cultivation condition affected evidently the residue level in pepper harvests. Residual pattern of KNF 1002 seemed to be comparable to strobilurin fungicides currently used.

• Korean Journal of Food Science and Technology
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• v.35 no.5
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• pp.764-768
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• 2003

Resveratrol is natually occurring phytoalexin compounds produced by grape berries, peanuts, and their products in response to stress such as fungal infection, heavy metal ions or UV irradiation. The objective of this study was to develop a reliable high-performance liquid chromatographic method for the quantitative determination of trans-resveratrol in grape and its products. The trans-resveratrol was separated isocratically on Nucleosil 100-5 C18 column, using a mobile phase containing acetonitrile : water (40 : 60, v/v), detected by UV detector at 306 nm and the flow rate was 0.3 mL/min. Under this analytical condition, the recoveries of trans-resveratrol in grape, wine, and grape juice were 92.35, 104.72, and 91.08, respectively. Limit of detection in grape, wine, and grape juice were 14.5 ng/g, 3.62 ng/mL, and 4.02 ng/mL. Also, limit of quantitation in grape, wine, and grape juice were 14.8 ng/g, 3.69 ng/mL, and 4.10 ng/mL. Assay values of 32 grape varieties, 9 wines, and 9 grape juices were ranged from trace amount to $207.1\;{\mu}g/100\;g$, from 5.4 to $275.7\;{\mu}g/L$, and from 63.3 to $751.6\;{\mu}g/L$, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.10
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• pp.1522-1527
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• 2010

This study was conducted to find out the minimal time needed for detection of Salmonella spp. which exist at very low concentration in foods by using real-time PCR. The sal-F and sal-R sequences were used as primers and sal-P was used as a probe. The detection limit of Salmonella spp. was $3.77{\times}10^2\;cfu/mL$ in buffered peptone water (BPW). Microbial growth was monitored after artificially inoculated Salmonella spp. into BPW. The obtained growth curve was well fitted with the equation, y＝$0.0127x^2$+0.5927x-0.4317 ($R^2$=0.99), if assuming that 1 cell exists in 25 g sample (0.04 cfu/mL). The microbial concentration will be reduced to 10 fold by adding BPW during sample treatment, so actual initial concentration at the starting point of enrichment is 0.004 cfu/mL. At this condition, real-time PCR detection would be possible only when microbial concentration increase occurs to exceed the detection limit (377 cfu/mL). The time needed for microbial increase was calculated from the growth curve equation as 7 hours and 20 minutes. Therefore the total time required for detection was less than 10 hours including the PCR operating time.

• Korean Journal of Food Science and Technology
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• v.31 no.1
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• pp.30-35
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• 1999

A simple, rapid, efficient method is for extraction of 13 synthetic water-soluble food colours (Tartrazine, Amarnth, Indigo carmine, New coccine, Sunset yellow FCF, Allura red AC, Eosine, Fast Green FCF, Brilliant Blue FCF, Erythrosine, Acid red, phloxine, Rose Bengal) by polyamide resin and for their quantitative by high performance liquid chromatography (HPLC). Colours (coal-tar dyes) were extracted with polyamide resin and then determinated by HPLC. The HPLC conditions using a reverse phase partition type column $(Nova-pak\;C_{18})$, photodiode array (PDA) detector and 1% Ammonium acetate / 60% acetonitrile in water as eluent, were acceptable for various kinds of colorants. By the use of the proposed method, a survey of coal-tar dyes was carried out on 20 samples and that were detected $4.76{\sim}133.47\;ppm$.

• Journal of the Institute of Convergence Signal Processing
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• v.5 no.2
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• pp.132-137
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• 2004

In this paper, the adaptive noise canceler using neural network with backpropagation is designed. The adaptive noise canceler using the least mean square algorithm has the large correlativity of the reference signal. The performance of the adaptive noise canceler shows the limitation when the information signal is relatively small to the noise. The system proposed in this paper plays an important role in denoising these signals. In addition, the experiments are carried out to analyze the effects of the number of hidden layers and nodes about the system. The performance of the proposed adaptive noise canceler is compared with that of the system which is used the least mean square algorithm.

• 한국방재학회:학술대회논문집
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• 2011.02a
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• pp.195-195
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• 2011

본 연구는 방염성능시험에 의한 필름 및 도료처리 시료를 대상으로 화재 시 발생하는 연소가스 중의 독성에 대하여 NES 713 방법에 의해 분석하고, 독성지수를 확인함으로써 연소 시 인체에 미치는 영향을 간접적으로 확인해 보고자 하였다. 기존 방염제품 중 필름과 도료처리 그리고 도료처리 후 기간이 경과한 시료의 3가지로 실험군을 구분하였으며, NES 713 방법으로 연소가스를 분석하고 독성지수를 평가하였으며, 추가로 기존의 국내 노출기준과의 비교를 수행하였으며, 국내 NES 713 방법을 활용한 선행연구들을 참조하였다. 발암가능 추정물질인 아크릴로니트릴은 총 18개의 시료에서 검출되었으며, 포름알데히드는 9개의 시료에서 검출되었다. 염화수소와 브롬화수소는 각각 5개의 시료들에서 검출된 반면에 도료처리된 시료에서는 각각 1개만이 검출되었다. 그 외 일산화탄소와 이산화탄소 그리고 질소산화물은 모든 시료에서 공통적으로 검출되었다. 독성지수는 최소 3.5에서 최고 9.4의 값을 갖는 것으로 나타났다. 본 연구에서 수행한 NES 713 분석결과와 독성지수 산출 및 기존 국내 노출기준과의 비교를 수행해 본 결과는 다음과 같았다. 첫째, 현행 국내 기준 상 방염제품의 독성시험 등에 관한 기준이 없고, 둘째, 방염제품에 대한 연소가스 독성 등을 평가한 선행연구가 제한적이라는 점에서 의미가 있을 것으로 사료된다. 하지만 독성지수의 적용이 국내에서 이루어지고 있지 않은 현실에서 산출도나 독성지수의 위해정도를 평가하기 어렵고, 시료의 수량이 총 18개로서 많지 않았다는 점에서 전체 방염제품의 연소가스 독성의 잠재적인 위험성을 추정할 수는 있지만 대표성을 갖기에는 문제점이 있다는 한계성을 벗어날 수 없었다. 따라서 향후 후속 연구에서는 보다 많은 방염제품들을 대상으로 무작위 선정을 통한 연구 설계 및 실험 연구가 수행된다면 보다 객관적이고 대표성을 지닌 연구결과를 도출할 수 있을 것으로 기대한다.

• Proceedings of the Korean Nuclear Society Conference
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• 1996.05c
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• pp.526-531
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• 1996

국내의 방사성 동위원소 (Radioisotope; RI) 사용기관에서 발생, 수거되어 현재 원자력연구소 저장고 내에 보관중인 폐기물중 가연분을 대상으로 소각실증실험을 수행하였다. 폐기물 발생일을 기준하여 최소 2년이상이 경과한 RI 폐기물을 소각한 결과 폐기물의 부피와 무게를 각각 약 1/250, 1/30로 줄일 수 있었으며, 소각운전중 배출기체의 농도는 환경관련법상의 배출허용기준치 이하, 배출방사선량은 검출기의 측정한계 이하였다. 방사성동위원소 추적자로 I-131 ($Na^{131}$ I형태, 총 2mCi)을 사용한 모의 폐기물을 소각한 결과 대부분의 방사능은 소각재에 잔존하기 보다는 휘발되어 배기체와 함께 거동하며 주로 포대여과기의 여과층에서 잡히는 것으로 나타났다.

• Pediatric Infection and Vaccine
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• v.22 no.2
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• pp.106-112
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• 2015

Purpose: The long-term administration of antibiotics interferes with bacterial culture in the middle ear fluids (MEFs) of young children with otitis media with effusion (OME). The purpose of this study is to determine whether molecular diagnostics can be used for rapid and direct detection of the bacterial pathogen in culture-negative MEFs. Methods: The specificity and sensitivity of both polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) to the lytA gene of Streptococcus pneumoniae were comparatively tested and then applied for pneumococcal detection in the clinical MEFs. Results: The detection limit of the PCR assay was approximately $10^4$ colony forming units (CFU), whereas that of LAMP was less than 10 CFU for the detection of S. pneumoniae. Both PCR and LAMP did not amplify nucleic acid at over $10^6$ CFU of H. influenzae or M. catarrhalis, both of which were irrelevant bacterial species. Of 22 culture-negative MEFs from children with OME, LAMP positivity was found in twelve MEFs (54.5%, 12/22), only three of which were PCR-positive (25%, 3/12). Our results showed that the ability of LAMP to detect pneumococcal DNA is over four times higher than that of PCR (P<0.01). Conclusions: As a high-resolution tool able to detect nucleic acid levels equivalent to <10 CFU of S. pneumoniae in MEFs without any cross-reaction with other pathogens, lytA -specific LAMP may be applied for diagnosing pneumococcus infection in OME as well as evaluating the impact of a pneumococcal conjugate vaccine against OME.