• Title/Summary/Keyword: 창성법

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Isolation of the Bacterium Pseudomonas sp. HC1 Effective in Inactivation of Tolaasin Produced by Pseudomonas tolaasii (버섯 세균성갈색무늬병원균(Pseudomonas tolaasii)의 분비 독소(tolaasin)를 저해하는 미생물 Pseudomonas sp. HC1)

  • Lee, Chan-Jung;Yoo, Young-Mi;Han, Ju-Yeon;Jhune, Chang-Sung;Cheong, Jong-Chun;Moon, Ji-Won;Suh, Jang-Sun;Han, Hye-Su;Cha, Jae-Soon
    • The Korean Journal of Mycology
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    • v.41 no.4
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    • pp.248-254
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    • 2013
  • A Gram-negative bacterium was isolated from mushroom media that markedly reduces the level of extracellular toxins (i.e., tolaasins) produced by Pseudomonas tolaasii, the most destructive pathogen of cultivated mushrooms. The HC1 strain was selected as detoxifying tolaasin by bioassay on potato and it was identified Pseudomonas sp. by the cultural, morphological and physiological characteristics, and analysis of the 16S rRNA. The isolated bacterium is saprophytic but not parasitic nor pathogenic to cultivation mushroom. The isolated bacterium for P. tolaasii cell, was sufficient for detoxification in vitro. Inoculation of the isolated bacterium prevents the development of bacterial disease in Pleurotus ostreatus, Flammunia velutipes and Agaricus bisporus. Control efficacy of brown blotch of strain HC1 treatment was 69, 68 and 55% on Agaricus bisporus, Flammulina velutipes and Pleurotus ostreatus, respectively. The suppressive bacterium may be useful in future for the development of biocontrol system and the construction of genetically modified edible fungi resistant to the disease caused by P. tolaasii.

Development of artificial cultivation conditions on Tricholoma gigantium (왕송이버섯(Tricholoma gigantium)의 인공재배를 위한 환경조건 구명)

  • Jang, Kab-Yeul;Park, Jeong-Sik;Cheong, Jong-Chun;Kong, Won-Sik;Yoo, Young-Bok;Jhune, Chang-Sung;Sung, Jae-Mo
    • Journal of Mushroom
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    • v.5 no.3_4
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    • pp.98-102
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    • 2007
  • Tricholoma gigantium, an edible mushroom, belongs to Tricholomataceae of Tricholoma and is distributed at Jeju-Do in Korea. It is also well-known as the medicinal mushroom in Taiwan. The cultivation method using the compost was developed in Korea in 1995. To develop a mass cultivation method and a superior strain, four strains were collected and tested. To establish the optimal cultivation conditions, various examinations were accomplished. Bag cultivation was more effective than box cultivation and the optimal relative humidity was more than 80%. Although the mycelial growth was tested in the substrate supplemented with different additives, such as rice bran and wheat bran, there's no significant difference between them. It suggested that the environmental conditions were more important than the substrate additives for cultivation.

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Phytopathogenicity of Burkholderia gladioli pv. alliicola CH1 and Production of PGase Isozymes (Burkholderia gladioli pv. alliicola CH1의 병원성 및 Polygaractronase Isozymes 생성)

  • Lee, Chan-Jung;Lee, Jong-Tae;Kim, Yeong-Tae;Jhune, Chang-Sung;Cheong, Jong-Chun;Park, Wan
    • Research in Plant Disease
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    • v.18 no.3
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    • pp.240-244
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    • 2012
  • Burkholderia gladioli pv. alliicola CH1 showed typical soft rot symptoms at higher than $20^{\circ}C$ but very weak soft rot symptoms at temperature under $10^{\circ}C$. Among the nine agro-chemicals, oxolinic acid WP, streptomycin + copper hydroxide WP and streptomycin WP were found to be effective for the inhibition of the pathogen in vitro. The results of scanning electron microscopic investigation showed that onion bulbs was macerated by infection of B. gladioli pv. alliicola CH1. B. gladioli pv. alliicola CH1 was able to produce polygalacturonase but did not produce pectin lyase and carboxymethylcellulase. In analysis of the polygalacturonase activity of the isolated pectin-degradation enzymes from B. gladioli pv. alliicola CH1 total protein, three activity bands 45 kDa, 35 kDa, and 29 kDa were detected by the direct (or in-gel) activity staining on SDS-PAGE.

금원대(金元代)까지의 상한론(傷寒論) 치법(治法)에 대한 연구(硏究) 지금원대대상한론치법적연구(至金元代對傷寒論治法的硏究)

  • Kim, Bong-Hyeon;Lee, Hae-Bok;Sin, Yeong-Il
    • Journal of Korean Medical classics
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    • v.18 no.4 s.31
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    • pp.155-165
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    • 2005
  • 진당시기대상한론치법적연구유(晋唐時期對傷寒論治法的硏究有): 왕숙화운용당시성행적(王叔和運用當時盛行的)‘한(汗), 토(吐), 하(下), 온(溫), 구(灸), 자(刺), 수(水), 화(火)’등팔법(等八法), 귀납료상한론적증치경험(歸納了傷寒論的證治經驗); 손사막근거자기적임상경험(孫思邈根據自己的臨床經驗), 파상한론적태양병편진행료(把傷寒論的太陽病篇進行了)‘이방명법(以方名法), 안법류증(按法類證)’, 저시해시기대상한론육경병치법적대표성적연구(這是該時期對傷寒論六經病治法的代表性的硏究), 저종연구유원시화력사적국한성(這種硏究有原始和歷史的局限性), 종중국의학대상한론육경병치법적연구력사고려(從中國醫學對傷寒論六經病治法的硏究歷史考慮), 저성위료후세적치법연구적선구자(這成爲了後世的治法硏究的先驅者), 구유비상대적영향(具有非常大的影響). 재송대대상한론치법적연구상(在宋代對傷寒論治法的硏究上), 기관건작용적의가유방안시화주굉(起關鍵作用的醫家有龐安時和朱肱). 타문대상한론치료원칙적천발(他們對傷寒論治療原則的闡發), 대륙경병적분석귀납(對六經病的分析歸納), 이급제시구체적치법상(以及提示具體的治法上), 도주출료공헌(都做出了貢獻). 방안시주장료응안인(龐安時主張了應按人), 지(地), 시제정치료적사상(時制定治療的思想); 주굉이(朱肱以)‘병유표본(病有標本), 치유선후(治有先後)’위치료원칙(爲治療原則), 여상한론상결합진행치료(與傷寒論相結合進行治療), 대후세의가산생료영향(對後世醫家産生了影響). 도료금원대성무기(到了金元代成無己), 류완소(劉完素), 왕호고등(王好古等), 대내경내용각자이사상관점(對內經內容各自以思想觀点), 분별안변증론치총결료육경병적치료규율(分別按辨證論治總結了六經病的治療規律), 동시대증후화방약진행료분석(同時對證候和方藥進行了分析), 천명료구체적육경병치법적병리전귀(闡明了具體的六經病治法的病理轉歸), 도유기독창성(都有其獨創性). 성무기용내경해석료상한론(成無己用內經解釋了傷寒論), 총결해기(總結解肌) 발한(發汗) 중제발한(重劑發汗) 해표행수(解表行水) 화해(和解) 공비 지열(止熱) 삼설등치법, 위후세대상한론치법적연구개벽료도로, 인이갱가명확화해적개념(因而更加明確和解的槪念), 병응용지금(幷應用至今). 류완소제창료주화론(劉完素提倡了主火論), 중시료상한론한(重視了傷寒論汗), 토(吐), 하삼법적연구(下三法的硏究), 창립료신량해표법(創立了辛凉解表法), 대후세온병치료적발전대래료흔대영향. 왕호고작위이수학파(王好古作爲易水學派), 운용장부적한열허실이론결합약미효능(運用臟腑的寒熱虛實理論結合藥味效能), 탐색료상한론육경병적치료규율(探索了傷寒論六經病的治療規律), 강조료양명병적익진액적치료원칙(强調了陽明病的益津液的治療原則), 대후세연구상한론치법급여료흔대적계발. 이상대상한론치법연구(以上對傷寒論治法硏究), 불근성위당시임상의학적선도(不僅成爲當時臨床醫學的先導), 이차성위료후세연구상한론치법적기초(而且成爲了後世硏究傷寒論治法的基礎).

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A Study on Critical Speed Enhancement of High-speed Train Passenger Car (고속열차 객차의 임계속도 향상에 관한 연구)

  • Jeon, Chang-Sung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.12
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    • pp.603-610
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    • 2016
  • Over 12 years have passed since the first commercial operation of a Korean high-speed train. Since then, the transport capacity of the high-speed lines has become almost saturated. Therefore, studies have been carried out to increase the operating speed of the trains in order to increase their transportation capacity. This study was carried out to improve the critical speed of the KTX-Sancheon, Korean high-speed train, in order to increase its operating speed. A dynamic analysis of the KTX-Sancheon train was performed using the contact data obtained from the wheel wear profiles that were measured from a KTX-Sancheon train in commercial operation. The analysis results were verified by comparing them with the measurement acceleration data obtained from KTX-Sancheon. The suspension parameters were optimized to improve the operation speed. The critical speed of KTX-Sancheon was increased by 9.4% after the optimization by the response surface method. The optimized suspension parameters are expected to be used for the new bogie design to increase the operating speed of KTX-Sancheon from 300km/h to 350km/h.

SWIR Application for the Identification of High-Grade Limestones from the Upper Pungchon Formation (풍촌층 상부 층준의 고품위 석회석 동정을 위한 SWIR 적용)

  • Kim, Yong-Hwi;Kim, Gyoo Bo;Choi, Seon-Gyu;Kim, Chang Seong
    • Economic and Environmental Geology
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    • v.49 no.5
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    • pp.335-347
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    • 2016
  • The mineralogical and geochemical characteristics of diverse carbonate rocks can be investigated by using VNIRSWIR(visible near infrared-short wavelength infrared) spectroscopic analysis as a rapid, nondestructive, and inexpensive tool. Comparing whole rock analysis to VNIR-SWIR spectroscopic analysis, the analytical method was investigated to estimate CaO contents, mud impurity, and whiteness of carbonate rocks involved in high-grade limestones in the field. We classify typical carbonate rocks in the upper Pungchon Formation in high-grade limestone mine area such as the Gangweon, Chungmu and Baegun mine in the Jeongseon area. The results show that powdered specimen has much higher reflectance than cutted specimen between the same sample. Whiteness is highly correlated with reflectance(0.99) for powdered specimen. The absorption of mineral mixtures shifts in position as a result of the mass ratio of calcite and dolomite in the Chungmu mine by changing to 75:25, 50:50, and 25:75. The absorption peak position in carbonate mixtures is highly correlated with CaO contents(0.98~0.99). Based on color system, the carbonate rocks are grouped into (milky) white, light grey, light brown, grey, and dark grey. The absorption peak position shifts from 2340 nm to 2320 nm as CaO contents decrease from 55.86 wt.% to 29.71 wt.%. We confirmed that absorption peak position shifts depending on the amount of Ca, which is bonded to $CO{_3}^{-2}$, Mg, and Fe contents replacing Ca. This result suggests that CaO contents in carbonate rocks can be considered to quantitative analysis in the field by spectroscopic analysis.

Pseudomonas azotoformans HC5 Effective in Antagonistic of Mushrooms Brown Blotch Disease Caused by Pseudomonas tolaasii (버섯 세균갈색무늬병균(Pseudomonas tolaasii)에 항균활성을 가지는 미생물 Pseudomonas azotoformans HC5)

  • Lee, Chan-Jung;Yoo, Young-Mi;Han, Ju-Yeon;Jhune, Chang-Sung;Cheong, Jong-Chun;Moon, Ji-Won;Gong, Won-Sik;Suh, Jang-Sun;Han, Hye-Su;Cha, Jae-Soon
    • The Korean Journal of Mycology
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    • v.42 no.3
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    • pp.219-224
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    • 2014
  • A gram-negative bacterium was isolated from spent substrate of Agaricus bisporus and showed marked antagonistic activity against Pseudomonas tolaasii. The bacterium was identified as Pseudomonas azotoformans by based on the cultural, biochemical and physiological characteristics, and 16S rRNA gene sequence. The isolated bacterium was saprophytic but not parasitic nor pathogenic to cultivation mushroom. The isolated bacterium for P. tolaasii cell was not sufficient for inhibition in vitro. Control efficacy of Pseudomonas azotoformans HC5 to brown blotch of P. tolaasii was 73, 78, and 71% on A. bisporus, Flammulina velutipes, and Pleurotus ostreatus, respectively. In the future, the suppressive bacterium may be useful for development of a biocontrol system.

Long-term evaluation of implant placed in sites grafed by lateral window approach on maxillary sinus;a 10-year retrospective study (측방 접근법에 의한 상악동 거상술을 이용하여 식립된 골내 임플란트의 10년간 후향적 연구)

  • Yon, Je-Young;Chae, Gyung-Joon;Jung, Ui-Won;Kim, Chang-Sung;Choi, Seong-Ho;Cho, Kyoo-Sung;Kim, Chong-Kwan;Chai, Jung-Kiu
    • Journal of Periodontal and Implant Science
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    • v.37 no.4
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    • pp.691-704
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    • 2007
  • Between 1997 May and 2007 May, One hundred and seventeen patients were treated. There were 129 cases of sinus elevation using a lateral window opening procedure and 258 implants placed simultaneously or delayed. The cumulative survival rate of the implants calculated. The implants were evaluated according to surgical site, quality and quantity of bone, graft material, membrane used, the length and diameter of the implant and complications. 1. The 10-year cumulative survival rate of the implants by sinus augmentation using lateral window approach was 96.90%. 2. There was no difference in the survival rate between the implant placed simultaneously with sinus elevation (one stage) and the procedure performed in the delayed procedure (two stage). 3. There was no difference in the survival rate according to the type and amount of graft materials. 4. There was no difference in the survival rate according to the implant site, bone quality and quantity. 5. There was no difference in the survival rate when the $CollaTape^{(R)}$ or Gore-Tex was placed in the window of the lateral wall. 6. There was no difference in the survival rate of the implant length and diameter. 7. The survival rate was as low as 75.00% when there were more than two complications. Implant placement with sinus augmentation using the lateral window approach is a predictable treatment method. Although the vertical height of residual ridge is insufficient and the quality of bone is poor, the normal survival rate of the implants would be expected if an appropriate graft material and membrane is used with greater effort to prevent complications.

Effects of Communication Improvement on Caregivers Education and Training on Aphasia (보호자 교육과 경험학습 훈련이 실어증 환자의 의사소통 개선에 미치는 효과)

  • Park, Hee-June;Chang, Hyun-Jin
    • Therapeutic Science for Rehabilitation
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    • v.8 no.2
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    • pp.79-88
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    • 2019
  • Objective : Aphasia interferes with communication between the patient and conversation partner. Adequate communication is essential not only for the patient but also for caregiver education and training Method : This study examined the benefits of parental education and group training in terms of improving the communication of six aphasic patients and their caregivers(family members). Caregiver education provided caregivers with information on stroke and aphasia, and group training was conducted according to the experimental learning cycle. Result : As a result, communication increased in terms of sending and receiving messages or interactive communication. Furthermore, the questionnaire analysis showed that caregivers learned more about aphasia and had confidence in using facilitation strategies. Conclusion : Giving educational opportunities to patients and caregivers promotes caregiver's knowledge and positively interacts.

Core-shell TiO2/Ag Nanoparticle Synthesis and Characterization for Conductive Paste (전도성 페이스트용 코어-쉘 TiO2/Ag 나노입자의 합성 및 특성 연구)

  • Sang-Bo, Sim;Jong-Dae, Han
    • Applied Chemistry for Engineering
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    • v.34 no.1
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    • pp.36-44
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    • 2023
  • Core-shell TiO2/Ag nanoparticles were synthesized by a modified sol-gel process and the reverse micelle method using acetoxime as a reducing agent in water/dodecylbenzenesulfonic acid (DDBA)/cyclohexane. The structure, shape, and size of the TiO2/Ag nanoparticles were investigated using X-ray diffraction (XRD), UV-visible spectroscopy, scanning electron microscope (SEM), transmission electron microscope (TEM), and thermogravimetric analysis (TGA). The size of TiO2/Ag nanoparticles could be controlled by changing the [water]/[DDBA] molar ratio values. The size and the polydispersity of TiO2/Ag nanoparticles increased when the [water]/[DDBA] molar ratio rose. The resultant Ag nanoparticles over the anatase crystal TiO2 nanoparticles exhibited a strong surface plasmon resonance (SPR) peak at about 430 nm. The SPR peak shifted to the red side with the increase in nanoparticle size. Conductive pastes with 70 wt% TiO2/Ag nanoparticles were prepared, and the pastes were coated on the PET films using a screen-printing method. The printed paste films of the TiO2/Ag nanoparticles demonstrated greater surface resistance than conventional Ag paste in the range of 405~630 μΩ/sq.