Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Phytopathogenicity of Burkholderia gladioli pv. alliicola CH1 and Production of PGase Isozymes

Burkholderia gladioli pv. alliicola CH1의 병원성 및 Polygaractronase Isozymes 생성

  • Lee, Chan-Jung (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Jong-Tae (Onion Research Institute, Gyeongnam Agricultural Research and Extension Services) ;
  • Kim, Yeong-Tae (Genomics Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Jhune, Chang-Sung (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Cheong, Jong-Chun (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Park, Wan (School of Life Sciences and Biotechnology, Kyungpook National University)
  • 이찬중 (국립원예특작과학원 버섯과) ;
  • 이종태 (경남농업기술원 양파연구소) ;
  • 김영태 (국립농업과학원 유전자분석개발과) ;
  • 전창성 (국립원예특작과학원 버섯과) ;
  • 정종천 (국립원예특작과학원 버섯과) ;
  • 박완 (경북대학교 생명과학부)
  • Received : 2011.10.05
  • Accepted : 2012.09.24
  • Published : 2012.09.30
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Abstract

Burkholderia gladioli pv. alliicola CH1 showed typical soft rot symptoms at higher than $20^{\circ}C$ but very weak soft rot symptoms at temperature under $10^{\circ}C$. Among the nine agro-chemicals, oxolinic acid WP, streptomycin + copper hydroxide WP and streptomycin WP were found to be effective for the inhibition of the pathogen in vitro. The results of scanning electron microscopic investigation showed that onion bulbs was macerated by infection of B. gladioli pv. alliicola CH1. B. gladioli pv. alliicola CH1 was able to produce polygalacturonase but did not produce pectin lyase and carboxymethylcellulase. In analysis of the polygalacturonase activity of the isolated pectin-degradation enzymes from B. gladioli pv. alliicola CH1 total protein, three activity bands 45 kDa, 35 kDa, and 29 kDa were detected by the direct (or in-gel) activity staining on SDS-PAGE.

양파 무름병 병원균인 Burkholder gladioli pv. allicola CH1을 양파에 접종했을 때 $20^{\circ}C$ 이상에서는 전형적인 양파 무름병 증상을 보이면서 심하게 부패하였지만, $10^{\circ}C$ 이하에서는 일부 조직이 무름증상을 보였지만 겉으로는 건전하게 보였다. B. gladioli pv. allicola CH1은 oxolinic acid WP, streptomycin + copper hydroxide WP와 Streptomycin WP에 강한 감수성을 보였다. 분비효소 중 펙틴질 분해효소의 활성은 검출할 수 있었지만, 섬유소 분해효소인 carboxymethylcellulase 활성은 검출되지 않았다. 그러나 펙틴질 분해활성 중에서 pectin(pectate) lyase의 활성은 검출되지 않고 polygalacturonase 활성만 검출할 수 있었다. PGA 액체배지에서 체외 효소는 18시간까지 급속한 효소반응을 보였으며, 24시간 후에 최고치를 가진 후 계속적인 활성을 가졌으며 효소의 생성은 균의 생육과 거의 일치하는 것으로 나타났다. PGA-SDS-PAGE에 의한 섬유소분해효소 직접활성 염색법을 이용하여 3종류의 polygalacturonase isozyme으로 추정되는 45 kDa, 35 kDa, 29 kDa 크기의 활성밴드를 관찰할 수 있었다.

Keywords

References

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