• The Journal of Dong Guk Oriental Medicine
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• v.6 no.1
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• pp.91-106
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• 1997

The purpose of this study is to observe tile protective effect of Leeganpunsusan on serum reaction and hepatic tissue in galactosamine treated rats. In this study, the experimental rats divided three group(Normal, Control and sample group). Under the same condition, normal and control groups were administered water, sample group was administered Leeganpunsusan for 15 days. The last day, both normal and control goups were injected to abdomen with galactosamine. The rates of lipid peroxide, SOD(activity) and xanthine oxidase(activity) were measured. The results were obtained as follows : Effects of the extract of Leeganpnnsusan on the hepatic lipid peroxidation, xanthine oxidase activity in VITRO, as compared with control group were significantly decreased with the level of concentration of extract prepared from Leeganpunsusan. In VIVO, the hepatic content of lipid peroxide, the rate of type changing(type D to O) and xanthine oxidase activity were significantly decreased in galactosamine-treated rats. Effect of on the hepatic cytosolic superoxide dismutase activity was significantly increased.

• The Journal of Internal Korean Medicine
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• v.21 no.2
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• pp.227-234
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• 2000

독성약물에 의한 급성신부전시 세뇨관세포의 물질 재흡수 장애에 대한 단삼(丹參) 추출액의 효과를 조사하였다. 토끼에 수은(HgCl2)을 10 mg/kg되게 피하 주사하여 급성신부전을 유발하였고, 단삼(丹參) 추출액의 효과는 수은을 주사하기 전 7일 동안 0.05% 액(液) 0.3 g/kg 용량을 경구 투여하여 관찰하였다. 수은을 주사하기 전 24시간 동안 요와 혈액을 채취하여 신장기능을 측정하여 대조기간(basal period)의 값으로 하였고, 수은을 주사한 후 24시간 동안 요와 혈액을 얻어 수은에 의한 신장기능 변화를 평가하였다. 수은을 처리한 후 사구체여과율이 대조값에 비해 감소하였고, 혈청내 creatinine 농도가 증가하였다. 이러한 결과들은 수은이 급성신부전을 유발하였음을 가리킨다. 수은을 처리한 동물에서 포도당 및 인산의 배설분율이 증가하였고, 이러한 변화는 brush-border membrane에서 물질의 이동장애와 Na-pump 활성의 감소에 기인하였다. 수은을 주사한 동물의 신장피질 절편에서 유기이온인 PAH와 TEA 이동이 억제되었다. 토끼의 신장조직에서 지질의 과산화가 수은을 주사한 후 증가하였다. 단삼(丹參) 추출액을 전 처리한 후 수은을 주사한 경우 수은에 의해 유발된 사구체여과율의 감소와 혈청내 creatinine 농도 증가 현상이 유의하게 완화되었다. 수은에 의한 세뇨관에서 물질의 재흡수 장애가 단삼(丹參) 추출액의 전처리에 의해 방지되었다. 단삼(丹參) 추출액은 수은에 의한 지질의 과산화를 억제하였다. 수은에 의한 급성신부전은 항산화제로 잘 알려진 DPPD에 의해 방지되었다. 이상의 결과를 종합하면 생체실험결과 수은에 의한 급성신부전의 유발과정에 지질의 과산화가 중요한 역할을 할 가능성을 보이고 있고, 단삼(丹參) 추출액은 수은에 의한 급성신부전을 방지하는 효과를 가지고 있으며, 그 효과는 단삼(丹參)의 항산화작용에 기인(起因)할 가능성이 많다.

• The Korean Journal of Pharmacology
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• v.31 no.3
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• pp.323-331
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• 1995

Monocyte/macrophage infiltration is the well known initial features associated with the development of glomerular disease including non-immune mediated nephropathy. Tumor necrosis factor ${\alpha}(TNF{\alpha})$, a cytokine produced primarily by monocyte/macrophage, exhibits similar effects as observed at the initial stages and during the progression of glomerular injury. Because the mesangial cells are target cells for glomerular injury, the present study examined the effect of $TNF{\alpha}$ on glomerular mesangial cell membrane lipid peroxidation as an index of cytotoxicity attributing to $TNF{\alpha}$. Primary culture of rat mesangial cell was established by incubation of glomeruli isolated from male Sprague-Dawley rat kidneys utilizing a standard sieving method. The levels of lipid peroxides in the mesangial cells were quantitated by malondialdehyde- thiobarbituric acid adduct formation. During an 8 hour incubation at $37^{\circ}C$, $TNF{\alpha}$ at 10 to 10,000 units/ml increased the levels of lipid peroxides dose dependently. Western blot analysis demonstrated that a short thermal stress induced heat shock response and the synthesis of heat shock protein 70(hsp70) in this mesangial cells. Further, this induction of hsp 70 prevented increase of lipid peroxides in the mesangial cells exposed to $TNF{\alpha}$. These data suggest that $TNF{\alpha}-induced$ lipid peroxidation in the mesangial cells may have pathophysiological relevance to glomerular injury and prior induction of heat shock response may play a role in the cellular resistance against $TNF{\alpha}-induced$ glomerular injury.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1992.05a
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• pp.35-35
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• 1992

산소라디칼-질병모델을 보다 간편한 방법으로 만들어 생화학적 발병 기전을 추구하므로서 신약창출에 필요한 기초자료를 제시하고자 본 실험을 실시하였다. 미세혈관의 혈류이상은 과잉의 활성산소를 생산하게되며 이로 인해 여러 종류의 난치성 질환 및 염증을 수반하는 많은 질병이 발병하게 된다. 일반적으로 흔히 이용되어지는 허혈-재관류모델이 아닌 환경독성물질을 활용한 병태모델을 만들어 이 모델조건에서 활성산소생성과 밀접한 관계를 갖는 잔틴 옥시데이즈(Xanthine Oxidase)의 변화와 과산화지질생성정도를 연관지어 검토하였다. 수은, 구리, 몰리브덴등의 금속이온들은 잔틴 디하이드로저네이즈(Xanthine dehydrogenase)로 부터 옥시데이즈(Oxidase)형으로의 전환을 촉진시켰으며 막독성의 지표로 이용되어지는 과산화지질의 생성도 현저히 증가시켰다. 또한 알데하이드류의 첨가실험에서도 잔틴산화효소의 형전환이 촉진되었으며 첨가한 알데하이드의 탄소수와 수소수 그리고 탄소와 탄소의 결합상태와도 밀접한 관계가 있음이 관찰되었다.

• Journal of the Korean Applied Science and Technology
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• v.2 no.2
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• pp.1-9
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• 1985

• The Journal of Internal Korean Medicine
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• v.19 no.2
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• pp.381-391
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• 1998

The present study was done to investigate the effects of Bombusae concretio Salicea (BCS) on cultured astrocyte cell system and lipid peroxidation in $A{\beta}25-35$ treatment conditions. Cell killing was significantly enhanced by addition of increasing concentrations of $A{\beta}25-35$. Pretreatment of BCS attenuated in cell killing enhanced by increasing concentrations of $A{\beta}25-35$. MDA level induced by $A{\beta}25-35$ treatment was significantly increased and the level was slightly reduced by pretreatment of BCS. The present study showed that $A{\beta}25-35$ strongly increased MDA level and the level was enhanced by addition of increasing concentrations of In conclusion, it was shown that $A{\beta}25-35$ is not only potent lipid peroxide inducer, but also cause protection of neurodegeneration induced by $A{\beta}25-35$. It can be concluded that the activation of antioxidative enzymes may be related to the inhibition of lipid peroxidative reactions. We cannot fully explain to effects of BCS at present; however, the ability of BCS to reduce cell killing and MDA level induced by $A{\beta}25-35$ suggest that BCS may be a protective agent for free radical generating compounds such as $A{\beta}25-35$.

• Journal of the Korean Applied Science and Technology
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• v.36 no.2
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• pp.600-608
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• 2019

The aim was to determine the physiological activity and antioxidant activity by lipid peroxidation inhibitory action of turmeric (Curcuma longa L.). Bioactive compound of total carotenoid $1.581{\pm}0.005mg$ ${\beta}$-carotene equivalents (BCE)/g dry weight. Total phenol content was the highest in the ethyl acetate (EA) extract, followed by chloroform:methanol (CM, 2:1, v/v) and 70% methanol extracts. Antioxidant effects (nitrogen oxide radical scavenging activity, nitrite scavenging activity, ${\beta}$-carotene bleaching assay, and lipid peroxidation inhibition action) of 70% methanol, CM, and EA extract of turmeric. Turmeric extracts yield were 70% methanol 16.54%, CM 5.64%, and EA 4.14%, respectively. Antioxidant activity of the samples exhibited a dose-dependent increase. However, in the current study, none of the samples evaluated showed activity as strong as the BHA (butylated hydroxyanisole) and trolox. Further, nitrite scavenging activity was the highest for the EA extract. As a result of this experiment, indicating their commercial value and potential applications in food and nutraceuticals.

• Journal of Food Hygiene and Safety
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• v.17 no.3
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• pp.124-128
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• 2002

The effect of different ethanol feeding protocols on the oxidative stress in liver and blain of rats was studied. The rats were fed 5%-ethanol solution in drinking water (5%-EtOH group) or 2.5g ethanol/kg body wt. once daily intragastrically (2.5g-EtOH group). The levels of thiobarbituric acid reactive substances (TRARS) and vitamin EI in the liver, cerebrum and cerebellum were measured. In the liver of 5%-EtOH group, the level of TBARS was not changed, whereas vitamin I was significantly increased compared to control group. In the liver of 2.5g-EtOH group, the level of TBARS was significantly increased compared to control group and the vitamin E concentration was not affected. The levels of TBARS were increased and the vitamin E concentrations were decreased significantly both in the cerebrum and cerebellum in 5%-EtOH group as well as in 2.5g-EtOH group. These results show that lipid peroxidation and vitamin E concentration in liver were varied according to the conditions of ethanol treatment, however, the vitamin E contents in cerebrum and cerebellum were affected by both ethanol intoxications used in this study.

• Korean Journal of Acupuncture
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• v.28 no.2
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• pp.23-36
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• 2011

목적 : 이 연구는 관절 및 심혈관계 질환 치료에 사용되는 송절(松節)(Pinus densiflora Gnarl)을 약침용 시료로 조제하여 본 약물의 항산화 효능을 규명하고자 하였으며 이를 다양한 시스템에서 검토하였다. 방법 : $FeCl_2$-ascorbic acid system에서 흰쥐 간조직의 지질과산화 반응을 관찰하였고, Fenton reaction system에서 자유기에 의한 plasmid DNA 분절을 유도하였다. 또한 deoxyribose assay를 통해 hydroxyl radical 소거능을 관찰하였고, NBT reduction assay로 superoxide radical 소거능을 검토하였다. 또한 human low-density lipoprotein(LDL)의 산화를 유도하기 위해 $CuSO_4$와 AAPH를 사용하였으며 relative electrophoretic mobility (REM) assay로 LDL 산화 억제 효능을 대조 항산화물질과 비교 검토하였다. 결과 : 송절 약침액은 자유기에 의한 간조직의 지질과산화(p < 0.01)및 DNA 분절을 현저하게 억제하였으며, hydroxyl radical, superoxide radical (p < 0.01), nitric oxide 및 peroxynitrite를 강하게 소거하였다. 또한 $CuSO_4$ ($IC_{50}=9.2{\pm}0.2\;{\mu}g/ml$)와 AAPH ($IC_{50}=34.8{\pm}5.1\;{\mu}g/ml$)에 의해 유도된 human LDL의 산화를 억제하였고, REM assay에서도 산화 억제 효능을 재확인할 수 있었다. 결론 : 송절 약침액은 활성산소종 및 활성질소종를 소거하였고, 지질과산화를 억제하였으며, 특히 human LDL의 산화적 손상을 방어하였다. 이에 본 약물은 자유기에 의한 심혈관의 산화적 손상을 효과적으로 보호할 것으로 판단된다.

• Applied Biological Chemistry
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• v.45 no.4
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• pp.218-222
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• 2002

Lycii Cordex Radicis extract (gigolpi) examined through SOS Chromotest showed a strong, dose-dependent antimutagenic effect on the tert-butyl hydroperoxide (t-BOOH) induced mutagenecity. Gigolpi revealed considerable superoxide anion radical scavenging activity under L-ascorbic $acid-CuSO_4$ system, but showed lower hydroxyl radical scavenging activity in photochemical test system. Hot-water gigolpi extract delayed protein oxidation, whereas lipid peroxidation of rat skin exposed to UVB radiation was inhibited. The results indicate that gigolpi possessing antioxidant activity against UVB-induced lipid peroxidation could be used as a raw ingredient for manufacturing functional cosmetics