Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Effect of Different Ethanol Treatments on the Oxidative Stress in Liver and Brain of SD Rats

알코올 투여 방법이 흰쥐의 간과 뇌에서 산화적 스트레스유발에 미치는 영향

  • 박민경 (청운대학교 식품영양학과) ;
  • 이영재 (제주대학교 수의학과)
  • Published : 2002.09.01
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Abstract

The effect of different ethanol feeding protocols on the oxidative stress in liver and blain of rats was studied. The rats were fed 5%-ethanol solution in drinking water (5%-EtOH group) or 2.5g ethanol/kg body wt. once daily intragastrically (2.5g-EtOH group). The levels of thiobarbituric acid reactive substances (TRARS) and vitamin EI in the liver, cerebrum and cerebellum were measured. In the liver of 5%-EtOH group, the level of TBARS was not changed, whereas vitamin I was significantly increased compared to control group. In the liver of 2.5g-EtOH group, the level of TBARS was significantly increased compared to control group and the vitamin E concentration was not affected. The levels of TBARS were increased and the vitamin E concentrations were decreased significantly both in the cerebrum and cerebellum in 5%-EtOH group as well as in 2.5g-EtOH group. These results show that lipid peroxidation and vitamin E concentration in liver were varied according to the conditions of ethanol treatment, however, the vitamin E contents in cerebrum and cerebellum were affected by both ethanol intoxications used in this study.

본 연구에서는 알코올 섭취 방법이 간과 뇌에서 산화적 스트레스 유발 정도에 미치는 영향을 알아보고자 횐쥐에 5%의 에탄올이 함유된 물을 1개월간 자유로이 섭취하게 하는 방법 또는 2.5g/kg의 에탄올을 1일 1회 1개월간 경구 투여하는 방법 등 알코올 투여 방법을 달리하고 간, 대뇌 및 소뇌에서 지질과산화 반응과 비타민 E의 함량 변화를 비교하였다. 5%-EtOH군의 간조직에서는 비타민 E의 함량이 증가하고 지질과산화물은 변화가 없었으며, 2.5g-EtOH군의 간조직에서는 비타민 E의 함량은 변화가 없으나 지질과산화물은 유의적으로 증가하였다. 뇌 조직에서는, 5%-EtOH군의 대뇌와 소뇌 모두에서 지질과산화물이 증가하고 비타민 E의 함량이 감소하였다. 또한, 2.5g-EtOH군의 대뇌와 소뇌에서도 지질과산화물이 증가하고 비타민 E의 함량이 감소하였으며 그 정도는, 5%-EtOH군에서 보다 큰 경향을 보였다. 결론적으로, 간에서의 지질과산화 반응 및 비타민 E의 함량은 알코올 투여 방법에 따라 다양하게 나타났으나 대뇌와 소뇌에서는 본 연구에서 실시한 투여 방법 모두에 의해 산화적 스트레스가 유발되었다.

Keywords

References

  1. Nordmann, R., Ribiere, C. and Rouach, H: Implication of free radical mechanisms in ethanol-induced cellular injury. Free Radic. Biol. Med., 12, 219-240(1992) https://doi.org/10.1016/0891-5849(92)90030-K
  2. Bohr, V, Anson, R.M., Mazur, S. and Dianov, G: Oxidative DNA damage processing and changes with aging. Toxicol. Lett., 102, 47-52(1998) https://doi.org/10.1016/S0378-4274(98)00280-X
  3. Diluzio, N.R. and Hartman, A.D: Role of lipid peroxidation in the pathogenesis of ethanol-induced fatty liver. Fed. Proc., 26, 1436-1442(1967)
  4. Niki, E., Saito, T., Kawakami, A. and Kamiya, A: Inhibition of oxidation of methyllinolate in solution by vitamin E and vitamin C. J. Biol. Chem., 259, 4177-4182(1984)
  5. Sadrzadeh, S.M., Nanji, A.A. and Meydani, M: Effect of chronic ethanol feeding on plasma and liver alpha- and gamma-tocopherol levels in normal and vitamin E-deficient rats. Relationship to lipid peroxidation. Biochem. Pharmacol., 47, 2005-2010(1994) https://doi.org/10.1016/0006-2952(94)90075-2
  6. Vatassery, G.T: Vitamin E and other endogenous antioxidants in the central nervous system. Geriatrics, 53, S25-S27(1998)
  7. Cardozo-Pelaez, F., Brooks, P.J., Stedeford, T., Song, S. and Sanchez-Ramos, J: DNA damage, repair, and antioxidant systems in brain regions: a correlative study. Free Radic. Biol. Med., 28, 779-785(2000) https://doi.org/10.1016/S0891-5849(00)00172-6
  8. Mihara, M., Uchiyama, M. and Fukuzawa, K: Thiobarbituric acid value on fresh homogenate of rat as a parameter of lipid peroxidation in aging, CCl_4 intoxication and vitamin E deficiency. Biochem. Med., 23, 302-311(1980) https://doi.org/10.1016/0006-2944(80)90040-X
  9. Vatassery, G.T. and Hagen D.F: A liquid chromatographic method for quantitative determination of $\alpha$-tocopherol in rat brain. Anal. Biochem., 79, 129-134(1997) https://doi.org/10.1016/S0300-9084(97)81504-0
  10. Westerberg, E., Friberg, M. and Akesson, B: Assay of brain tocopherols using high performance liquid chromatography. J. Liquid Chromatography, 4, 109-121(1981) https://doi.org/10.1080/01483918108064801
  11. Ingelman-Sundberg, M., Ronis, M.J., Lindros, K.O, Eliasson, E. and Zhukov, A: Ethanol-inducible cytochrome P4502E1 :regulation, enzymology and molecular biology. Alcohol Alcohol Suppl., 2, 131-139(1994)
  12. Moore, D.R., Reinke, L.A. and McCay, P.B: Metabolism of ethanol to 1-hydroxyethyl radicals in vivo :detection with intravenous administration of alpha-(4-pyridyl-1- oxide)-N-t-butylnitrone. Mol. Pharmacol., 47, 1224-1230(1995)
  13. Cunnmgham, C.C. and Bailey, S,M: Ethanol consumption and liver mitochondria function. Biol. Signals Recept., 10, 271-282(2001) https://doi.org/10.1159/000046892
  14. Shaw, S. and Jayatilleke, E: The role of cellular oxidases and catalytic iron in the pathogenesis of ethanol-induced liver injuiy. Life Sci., 50, 2045-2052(1992) https://doi.org/10.1016/0024-3205(92)90570-F
  15. Whitfield, J.B., Zhu, H., Heath, A.C., Powell, L.W. and Martin, N.G: Effects of alcohol consumption on indices of iron stores and of iron stores on alcohol intake makers. Alcohol Clin. Exp. Res., 25, 1037-1045(2001) https://doi.org/10.1111/j.1530-0277.2001.tb02314.x
  16. Meydani, M., Seitz, H.K., Blumberg, J.B. and Russell, R.M: Effect of chronic ethanol feeding on hepatic and extrahepatic distribution of vitamin E in rats. Alcohol Clin. Exp. Res., 15, 771-774(1991) https://doi.org/10.1111/j.1530-0277.1991.tb00598.x
  17. Wisniewska-Knypl, J.M. and Wronska-Nofer, T: Biological markers of oxidative stress induced by ethanol and iron overload in rat. Int. J. Occup. Med. Environ. Health., 7, 355-363(1994)
  18. Coudray, C., Richard, M.J., Faure, H. and Favier, A: Blood and liver lipid peroxide status after chronic ethanol administration in rats. Clin. Chim. Acta., 129, 35-45(1993)
  19. Rouach, H., Fataccioli, V., Gentil, M., French, S.W, Morimoto, M. and Nordmann, R: Effect of chronic ethanol feeding on lipid peroxidation and protein oxidation in relation to liver pathology. Hepatology, 25, 351-355(1997) https://doi.org/10.1002/hep.510250216
  20. Eaton, S., Record, C.O. and Bartlett, K: Multiple biochemical effects in the pathogenesis of alcoholic fatty liver. Eur. J. Clin. Invest., 27, 719-722(1997) https://doi.org/10.1046/j.1365-2362.1997.1780727.x
  21. Bjorneboe, A., Bjomeboe, G.E., Hagen, B.F. and Drevon, C.A: Acute and chronic effects of ethanol on secretion of alpha-tocopherol from primary cultures of rat hepatocytes. Biochim. Biophys. Acta, 922, 357-363(1987) https://doi.org/10.1016/0005-2760(87)90059-2
  22. Anandatheerthavarada, H.K., Shankar, S.K., Bhamre, S., Boyd, M.R., Song, B.J. and Ravindraath, V: Induction of brain cytochrome P-450IIE1 by chronic ethanol treatment. Brain Res., 601, 279-285(1993) https://doi.org/10.1016/0006-8993(93)91721-4
  23. Sohda, T., Shimizu, M., K.amimura, S. and Okumura, M: Immunohistochemical demonstration of ethanol-inducible P450 2E1 in rat brain. Alcohol Alcohol Suppl., 1B, 69-75(1993)
  24. Rouach, H., Houze, P., Gentil. M., Orfanelli, M.T. and Nordmann, R: Changes in some pro-and antioxidants in rat cerebellum after chronic alcohol intake. Biochem. Pharmacol., 53, 539-545(1997) https://doi.org/10.1016/S0006-2952(96)00770-8
  25. Renis, M., Calabrese, V., Russo, A., Calderone, A., Barcellona, M.L. and Rizza V. Nuclear DNA strand breaks during ethanol-induced oxidative stress in rat brain. FEBS Lett., 390, 153-156(1996) https://doi.org/10.1016/0014-5793(96)00647-3
  26. Agar, E., Bosnak, M., Amanvermez, R., Demir, S., Ayyildiz, M. and Celik, C: The effect of ethanol on lipid peroxidation and glutathione level in the brain stem of rat. Neuroreport, 10, 1799-1801(1999) https://doi.org/10.1097/00001756-199906030-00032
  27. Martin, A., Prior, R., Shukitt-Hale, B., Cao, G. and Joseph, J.A: Effect of fruits, vegetables, or vitamin E-rich diet on vitamins E and C distribution in peripheral and brain tissues. J. Gerontol., 55, B144-6151(2001)