• 현장농수산연구지
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• 제21권1호
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• pp.71-80
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• 2019

우리나라 자원식물로 오랫동안 한약재로 이용하고 있는 청미래덩굴, 약모밀, 삼백초, 줄풀 총 4종의 야생화를 산업화하기 위해 실험을 하였다. 청미래덩굴, 약모밀, 삼백초는 뿌리를 사용하였고 줄풀은 잎을 사용하였다. 추출은 50% ethyl alcohol에서 하였고, 이를 농축하여 재료를 냉동보관 한 후 100 ppm의 저농도를 투입했다. 이상의 방법으로 4종의 야생화를 이용한 항균력과 항진균력을 살펴본 결과는 다음과 같다. 1. 청미래덩굴, 삼백초, 약모밀, 줄풀을 알코올로 추출 후 분말화하여 살균과 진균 test를 한 결과 Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa에 대한 억제력은 약모밀에서 가장 높게 나타났고 줄풀은 억제력은 어느 정도 입증되었다. 2. 진균 종류인 Candida albicans, Aspergillus niger, Aspergillus fumigatus, Cryptococcus neoformans에 대한 항진균 실험에서는 Candida albicans에 대한 억제력은 미미하였지만 일부 확인하였으며 다른 진균에 대한 억제력은 전혀 나타나지 않았다.

• 한국의류산업학회지
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• 제21권2호
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• pp.231-236
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• 2019

This study investigated the proper dyeing conditions, color fastness and functionality of wool fabrics dyed with Zizania latifolia Turcz. We also tried to improve light fastness through treatment with benzophenone ultraviolet absorber. The dyeing of wool fabrics using Zizania latifolia Turcz was good even without pretreatment or mordanting treatment. Optimal wool fabric dyeing conditions were colorant concentration of 200% (o.w.f.), dyeing temperature of $100^{\circ}C$, dyeing time of 80 minutes and a dye bath pH of 3. Color fastness of dyed wool fabrics to washing, rubbing, perspiration and light was 4-5, 5, 4-4-5 (acidic), 4-5 (alkaline) and 2 respectively. The results after treatment with ultraviolet absorber for improving the fastness of daylight were improved to 3-4 grade. The UV protection rate were increased after dyeing and the deodorization of ammonia gas improved to 98%. Bacterial reduction rate (Staphylococcus aureus) of wool fabrics was excellent at 99.9%. All dye fastness (except for light fastness) was excellent; in addition, the functionality of wool fabrics dyed with Zizania latifolia Turcz also improved. The results are expected to be applied to various fields because they indicate excellent results after treatment with ultraviolet absorber for improving the fastness of daylight.

• Current Research on Agriculture and Life Sciences
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• 제33권2호
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• pp.57-63
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• 2015

안전한 천연물을 이용한 천연항산화제 개발 연구의 일환으로, 오크라, 엉컹퀴, 엄나무 및 줄풀 등 4종의 천연 약용식물 추출물의 항산화 활성을 측정해보았다. 추출물의 총 phenolic compounds 함량은 오크라와 엉컹퀴는 50% ethanol, 엄나무는 40% ethanol, 줄풀은 60% ethanol에서 최대용출을 나타내었으며, water 추출물에서 2.72~34.15 mg/g, ethanol 추출물에서 2.83~34.23 mg/g의 용출율을 나타내었다. 4종류 약용식물 water과 ethanol 추출물의 DPPH radical 소거능은 $50{\mu}g/mL$의 저농도에서 모두 74% 이상의 높은 소거활성을 나타내었다. ABTS radical cation decolorization을 측정한 결과, 4종의 약용식물 water 추출물과 ethanol 추출물 $100{\mu}g/mL$의 농도에서 모두 88% 이상의 높은 저해율을 나타내었다. 지용성 물질에 대한 항산화 효과를 측정하기 위해 antioxidant protection factor(PF)를 측정한 결과, 엉컹퀴 water과 ethanol 추출물 $50{\ddot{I}}g/mL$의 저 농도에서 각각 1.73과 1.76 PF로 다른 약용식물 보다 높은 항산화효과를 나타내었다. TBARs 생성 억제율을 측정한 결과 오크라를 제외한 3종의 약용식물 추출물 $50{\mu}g/mL$ phenolic 농도에서 80% 이상의 생성억제 효과를 나타내었다. 이러한 결과에 따라 다양한 약용식물 추출물이 항산화 및 기능성 식품 소재로 활용이 가능하다고 판단되었다.

• 한국염색가공학회지
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• 제27권1호
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• pp.96-104
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• 2015

This study examined the pretreatment effect, appropriate dyeing conditions, color fastness and functionality of cotton fabrics dyed with Zizania latifolia Turcz. Dye uptake was substantially increased by pretreatment and chitosan pretreatment was much more effective than tannic acid pretreatment. Optimal dyeing conditions were colorant concentration of 100%, dyeing temperature of $100^{\circ}C$, dyeing time of 80 minutes and dyebath pH of 5.5. Color fastness of chitosan pretreatment and dyed cotton to washing, rubbing, perspiration and light was 4, 5, 3-4(acidic), 3(alkaline) and 3 respectively. Color fastness of tannic acid pretreatment and dyed cotton to washing, rubbing, perspiration and light was 4, 5, 3-4(acidic), 4-5(alkaline) and 4 respectively. Deodorization rates of ammonia(NH3) were 99.9% in both cases. UV protection rate were 98.3% of UV-A and 98.5% of UV-B in case of chitosan pretreatment. UV protection rate were 98.2% of UV-A and 98.5% of UV-B in case of tannic acid pretreatment. UV protection factor(UPF) was 50+ in both cases. Reduction rate of Staphyloccus aureus were 99.9% in both cases. Therefore Zizania latifolia Turcz. could be used a new functional colorant.

• 한국염색가공학회지
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• 제30권2호
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• pp.150-157
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• 2018

The purpose of this study is to investigate the proper dyeing condition, color fastness and functionality of silk fabrics dyed with Zizania latifolia Turcz. FT-IR spectrum analysis suggests that colorants of Zizania latifolia Turcz. are tannins. The dyeing of the silk fabrics using Zizania latifolia Turcz. was very good even without pretreatment or mordanting treatment. Optimal dyeing conditions of silk fabrics were colorant concentration of 200%(o.w.f.), dyeing temperature of $100^{\circ}C$, dyeing time of 60 minutes and dyebath pH of 5.5. Color fastness of dyed silk fabrics to washing, rubbing, perspiration and light was 4~4-5, 5, 4~4-5(acidic), 4-5(alkaline) and 2 respectively. UV protection rate and deodorization rate of silk fabrics dyed with Zizania latifolia Turcz. were improved. Reduction rate(Staphyloccus aureus) of silk fabrics was excellent at 98.3%. As a result, it was confirmed that the Zizania latifolia Turcz. could be used as an eco-friendly functional natural dye.

• 한국산림과학회지
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• 제103권2호
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• pp.240-247
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• 2014

본 연구는 황폐계류 복원지의 식생변화에 대한 시계열 분석을 통하여 복원기법 및 효과에 대해 고찰하고자 수행 되었으며, 수질정화습지와 월류보, 수생식물 복원지에서 복원 전, 후 식생변화를 2년간 모니터링 하였다. 복원 1년 후 월류보에서 초본류 증가와 수질정화습지에서 줄풀의 피복도 증가가 조사되었다. 식생 피복률은 복원직후 10~30% 감소되었고, 복원 1년 후 복원 전 수준으로 조사 되었다. 황폐계류 복원지의 식생변화는 복원 후 1년 경과시점부터 서식종수, 귀화률, 피복률이 증가하는 것으로 조사되었다.

• 동의생리병리학회지
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• 제19권4호
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• pp.1062-1067
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• 2005

The purpose of this study was to examine the inhibition effect of Zizania latifolia that has been used heart disease, Diabetes Mellitus and Skin disease for a long time on apoptosis induced by $H_2O_2$ in Neuro2A cell. Neuro2A cells were cultivated in RPMI(GibcoBRL) with $5\%$, FBS and treated with $H_2O_2$, and Zizania latifolia. We measured the cell viability and analyzed DNA fragmentation. Activity of PARP, Cytochrome C, caspase-9, caspase-3, p53, p21, Bax and Bcl-2 in the cell was examined by using western blot. The cell viability in Zizania latifolia treatment (60ug/ml<) decreased significantly compared with that of none treatment. (p<0.001) Zizania latifolia increased cell viability about twice as much as that being injury by $H_2O_2$. (Zizania Latifolia 20ug/ml, $H_2O_2$ 200uM, P<0.001) DNA fragmentation developed by $H_2O_2$, but was not developed in Zizania latifolia treatment. PARP, Cytochrome C, caspase-9 and caspase-3 activated all by $H_2O_2$ but were not activated in Zizania latifolia treatment.. P53, P2l and Bu activated by $H_2O_2$, and Bcl-2 got into inactivation. But the opposite results appeared in Zizania latifolia treatment. In conclusion, these results suggest that Zizania latifolia inhibit the development of DNA fragmentation and apoptosis by $H_2O_2$ and the antioxidant action of Zizania latifolia is effective. More researches about effect of Zizania latifolia are considered to need.

• 동의생리병리학회지
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• 제20권1호
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• pp.149-155
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• 2006

To prevent human body injury from oxidative stress, antioxidants are very important and many research about antioxidants are generally being conducted. Hydrogen peroxide($H_2O_2$) that is one of vitality oxygen species has been seen that cause various diseases, DNA damage and gene change. The purpose of this study was to examine the inhibition effect of Zizania latifolia Rhizoma on apoptosis induced by $H_2O_2$ in Neuro2A cell. Neuro2A cells were cultivated in RPMI(GibcoBRL) with 5% FBS and treated with $H_2O_2$ and Zizania latifolia Rhizoma. We measured the cell viability and analyzed DNA fragmentation. Activity of PARP, Cytochrome C, caspase-9, caspase-3, p53, p21, Bax and Bcl-2 in the cell was examined dy using western blot. The results obtained were as Follows: The cell viability in Zizania latifolia Rhizoma treatment (60ug/ml<) decreased significantly compared with that of none treatment. (P<0.001) Zizania latifolia Rhizoma increased cell viability about twice as much as that being injury by $H_2O_2$. (Zizania Latifolia Rhizoma 20ug/ml, $H_2O_2$ 200uM, P<0.001) DNA fragmentation developed by $H_2O_2$, but was not developed in Zizania latifolia Rhizoma treatment. PARP, Cytochrome C, caspase-9 and caspase-3 activated all by $H_2O_2$ but were not activated in Zizania latifolia Rhizoma treatment. P53, P2l and Bax activated dy $H_2O_2$, and Bcl-2 got into inactivation. But the opposite results appeared in Zizania latifolia Rhizoma treatment. In conclusion, these results suggest that Zizania latifolia Rhizoma inhibit the development of DNA fragmentation and apoptosis by $H_2O_2$ and the antioxidant action of Zizania latifolia Rhizoma is effective. More researches about effect of Zizania latifolia Rhizoma are considered to need.

• 한국잡초학회지
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• 제2권2호
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• pp.169-174
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• 1982

동진강(東津江) 유역(流域)의 관개(灌漑), 배수로(排水路) 13개소(個所)를 선정하여 잡초(雜草)의 분포(分布)를 조사(調査)하였고 설문(說問)을 통하여 전국에서 53개 농지개량조합(農地改良組合)을 대상으로 관할구역내(管割區域內) 수로(水路)에서 문제(問題)되는 수생잡초(水生雜草)의 발생정도(發生程度)를 조사(調査)하였으며 제초제(除草劑)에 의한 수생잡초(水生雜草)의 방제법(防除法)을 검토(檢討)하였다. 1. 동진강(東津江) 유역(流域)의 관개(灌漑), 배수로(排水路)에서 발생(發生)된 잡초(雜草)는 나도겨폴, 붕어마름, 줄폴, 개연꽃, 갈대, 나사말, 마름, 물수세미와 말즘이었다. 2. 전국적인 수생잡초(水生雜草)의 발생정도(發生程度)를 살펴 보면 수로(水路) 변잡초중(邊雜草中)에서는 줄풀이 가장 많았고, 갈대, 나도겨풀의 순으로 발생(發生)되었다. 수중잡초중(水中雜草中)에서는 붕어마름이 가장 많이 발생(發生)되었고 물수세미, 말즘, 나사말, 말도 비교적 많이 발생(發生)되었다. 3. 나도겨풀은 Paraquat 73.5g/10a, Glyphosate 91.5g/10a와 Fluridone 74.7g/10a에서, 줄풀은 Paraquat 220.5g/10a, Glyphosate 366.0g/10a 와 Fluridone 74.7g/10a에서 고사(枯死)되었다. 붕어마름과 말즘은 2, 4, 5-TP 540g/10a와 Fluridone 100g/10a에서 고사(枯死)되었으나 2, 4-D 600g/10a에서는 80% 방제(防除)되었다.