• Journal of the Society of Cosmetic Scientists of Korea
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• v.48 no.4
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• pp.321-330
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• 2022

This study attempted to establish an optimal multi-compound simultaneous analysis method that can secure reliable results for 15 - preservatives, 2 - sun screens and 1 - antioxidants of cosmetics using HPLC-PDA. Since the potential of hydrogen (pH) in the mobile phase affects the acid dissociation constant (pKa) of the preservatives, and the peak retention time shift and area change were observed. The peak separation condition was established by adjusting the pH to 0.1% H₃PO₄ addition (mL) when preparing the mobile phase. As a results of method validation, the linearity correlation coefficient (R²) of above 0.999 were obtained, and accuracy 87.9 ~ 101.1%, 0.1 ~ 7.6% precision for two types of cosmetics (cream and shampoo). It was found that the limit of detection (LOD) was 0.1 ~ 0.2 mg/kg and the limit of quantitation (LOQ) was 2.0 ~ 4.0 mg/kg. In addition, it was possible to simultaneously separate p-anisic acid, a natural compound that was difficult to separate in HPLC due to the small difference from methylparaben, a synthetic preservatives. Through this study, it will be effectively used to secure quality control and safety for compound that need restrictions on use cosmetics.

• Proceedings of the Korea Water Resources Association Conference
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• 2022.05a
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• pp.269-269
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• 2022

최근 낙동강 하구 기수생태 복원에 있어서 중요한 요소 중 하나는 하굿둑 외해측의 보다 높은 정도를 가지는 조석예보치 산정과 이를 통해 하굿둑 방류량과 해수 유입량을 추정하여 주변 환경 등을 예측할 수 있다. 기수생태 복원이 본격으로 논의가 진행 전인 2016년까지는 하구에서 수km 떨어진 기존 조위관측소(부산 및 가덕도)를 활용하여 하류수위를 예측하여 왔지만 조위 높이와 위상 차이로 인하여 활용이 용이하지 않다. 따라서, 낙동강 하굿둑 인접 외해역에서 조석 영향을 받는 수위관측치를 이용하여 조석조화분해를 통해 조위 예측을 보다 정밀하게 산정하는 것이 필요하다. 연구방법으로는 낙동강 하굿둑 외해역에서 관측된 2016년, 2017년 각각 1년간 10분간격으로 관측자료의 저장상태 및 이상자료 유무를 확인하고, 조석조화분해 프로그램인 TASK2000(Tidal Analysis Software Kit) Package를 이용하여 2016년, 2017년 낙동강 하굿둑 인접 외해역에서 관측된 조위자료를 각각 조석조화분해한 결과로 관측조위와 예측조위 비교하였고, 관측조위와 예측조위를 뺀 성분인 조석잔차성분을 구했다. 조화분해결과, 낙동강 하굿둑 외해역은 일반적인 연안역의 조석과는 달리 하천수의 유출, 배수갑문의 조작, 연안사주지형에 의한 조석변형 등 매우 복잡하고 불규칙적인 특성인 기상성분(기압, 바람 등)에 의한 교란을 고려한다면 예측정확도가 상당부분 확보되는 것으로 나타났다. 또한 장주기 성분과 비선형 조석성분의 크기를 비교해 볼 때 거의 편차가 없이 나타나 조석조화상수를 이용한 예보 가능성을 확인할 수 있었다. 조위검증은 2016년의 1년치의 조석자료를 이용하여 조화분해된 조화상수 63개를 이용하여 2017년의 조석 예보치를 산정하였으며, 이를 2017년의 낙동강 하굿둑 외해역의 조석관측치와 조석예측치를 1대 1 비교하는 방식으로 검증하였고, 이들의 상관관계를 파악하기 위하여 두 성분에 대하여 Regression Analysis를 수행하여 예측조위와 관측조위 사이에는 Pre=0.9535×Obs+0.396과 같은 관계식이 성립하는 것으로 분석되었다. 또한, 두 성분간의 상관도는 0.9535로 높게 나타났다. 조위예측 프로그램인 TASK2000 Package 중 MARIE를 이용한 조위예측 프로그램의 신뢰도가 매우 높은 것으로 판단되고, 해당년도 조위예측 시에는 가능하면 직전년도의 1년 조석관측자료를 조화분해하고 얻어진 조화상수를 이용하여 조위예측을 실시하면 보다 정확한 자료를 얻을 수 있다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.7
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• pp.834-840
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• 2008

In order to improve the use of pumpkin seed, the present study was performed to isolate compositions of the bitter components which were not seen in pumpkin seed itself but newly biosynthesized during germination of the seed. The compositions isolated were then further purified by TLC and preparative HPLC in which a fraction with Rf 0.73 and RT 10.3 was obtained. Cucurbitacin E with molecular weight of 557 from the fraction was finally identified by subsequent structural analysis of LC-MS/MS. The production of cucurbitacin E peaked with 224.7 mg/kg at 4 days of germination at $20^{\circ}C$ with the water supply at ntervals of 48 hrs in the darkness, while that of cucurbitacin E reached 146.7 mg/kg in the brightness. In vitro-cell based assays demonstrated that the isolated and purified cucurbitacin E inhibited proliferation of A549 lung cancer cells and suppressed expression of the IL-$1{\beta}$- or PMA-induced cyclooxygenase-2, an inflammatory protein in A549 cells, suggesting its anti-proliferative and anti-inflammatory activities.

• Proceedings of the Korean Vacuum Society Conference
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• 2010.02a
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• pp.234-234
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• 2010

최근 CMOS 소자 크기가 축소됨에 따라 소스와 드레인 영역에서의 접촉저항을 줄이기 위하여, 실리사이드 공정이 많이 연구되고 있다. 실리사이드 물질로서 NiSi는 낮은 저항률과 낮은 실리콘 소모, 낮은 공정온도, 등의 장점을 가지고 있다. 그러나, 실리사이드 형성으로 인한 나노소자의 소오스/드레인에서정션(junction) 누설전류의 증가는 큰 문제가 되므로 실리콘과 실리사이드 계면의 특성이 중요하다. 본 연구에서는 니켈을 이용한 실리사이드 형성시 계면 활성제인 에틸 요오드를 이용하여 실험을 진행하였다. 금속 유기 전구체인 MABONi을 사용하여 ALD 방식으로 증착 한 니켈 박막과 니켈 핵 형성시 계면활성제인 에틸요오드의 처리 방법에 따른 Ni-silicide 박막의 특성을 비교, 분석하였다. 먼저 자연산화막을 건식식각으로 제거한 뒤, 첫 번째 샘플에서는 10회의 주기로 초기 니켈을 증착한 뒤, 에틸요오드로 니켈의 표면 위를 처리하고, 다시 200회의 주기로 니켈을 증착하였으며, 두 번째는 첫 번째 방식에서 에틸요오드 주입 시 동시에 수소도 함께 주입하였다. 세 번째는 비교를 위해 에틸요오드 처리를 하지 않고 니켈 박막만을 증착 하였다. 이어서, 각 샘플을 급속 열처리 장비에서 $400^{\circ}C$부터 $900^{\circ}C$까지 각각 30sec간 열처리를 진행후, 반응하지 않은 잔여 니켈을 제거한 후, XRD(x-ray diffraction), AES(auger), 그리고 4-point probe 등을 이용하여 형성된 실리사이드의 특성을 분석하였다. 에틸요오드와 함께 수소를 주입한 경우 계면에서의 산소 불순물과 카본 성분이 효과적으로 제거되어 $400^{\circ}C$에서 $2.9{\Omega}/{\Box}$ 의 낮은 면저항을 가지는 NiSi가 형성되었고 모든 온도구간에서 다른 샘플에 비하여 가장 낮은 면저항 분포를 보였다. 이는 분해 흡착된 요오드에 의한 계면 특성 향상과 카본 성분이 포함된 잔여물들이 수소처리에 의해 효율적으로 제거되어 실리사이드의 특성이 향상되었기 때문이다. 계면활성제를 사용하지 않은 경우에는 $500^{\circ}C$에서 NiSi가 형성되었다. 반면에 에틸요오드로만 표면을 처리한 경우에는 니켈과 실리콘 계면에서의 카본 성분에 의하여 silicidation 이 충분히 일어나지 않았다. 이러한 결과는 향후 45nm 이하의 CMOS 공정상에서 소스와 드레인의 낮은 누설전류를 가지고, 접촉저항을 줄이기 위한 니켈 실리사이드 형성에 큰 도움을 줄 것으로 기대된다.

• Korean Journal of Remote Sensing
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• v.13 no.1
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• pp.1-12
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• 1997

The western equatorial Pacific Ocean, where sea surface temperature is the warmest on the globe, is characterized by numerous convective systems and large annual precipitation. In this region, the cloudiness data with tops higher than 8km level obtained from the GMS-IR data are used to investigate the diurnal variation of cloudiness. The amplitude and phase of diurnal and semi-diurnal cycles are mainly investigated to examine details on the temporal and spatial structure of clouds. Cloudiness variation has typical cycles and each cycle is associated with the air-sea interactive phenomena. Spectral analysis on the cloudiness time series data indicates that 30-60 day, 17-20day, 7-8 day, diurnal and semi diurnal cycle are peaked. During Northern Winter and Southern Summer, the large cloudiness exsists over New Guinea, the adjacent seas of North Australia, and the open oceanic regions east of $160^{\circ}$E. Cloudiness diurnal variability over the lands and their adjacent seas is about 2.0 times larger than that over the open sea regions. That may be due to the difference of specific heat between the land and sea. The maximum and minimum cloudiness appeared at 18:00 and 09:00 hours over the land, and at noon and 21:00 hours over the sea, respectively. The amplitude of diurnal component over the land is 4,7 times larger than that of semi-diurnal component, and 1.5 times over the sea.

• Water for future
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• v.22 no.2
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• pp.201-211
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• 1989

Using the storm data which was augmented by the stochastic correlation with it's neighbors, the multiquadric equation of random surface of total storm depth is constructed. And to separate the local components from it's regionals and find the regional characteristics, a double Fourier analysis was applied to the total depths of storm data. The local components, storm residuals of each storm was assumed to be homogeneous random field and investigated with it's autocorrelation function. For the practical application, isotropic was assumed and that was identified with emprical data. Coefficients of normalized autocorrelation for all storms showed similar apperance. Using this emprical result, an example of the radial spectral distribution function which represints the spatial characteristics of rainfall over Han River Basin during 1975-1983 is presented.

• KOREAN POULTRY JOURNAL
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• s.18
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• pp.56-61
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• 1971

11종의 시판 부로일러용 배합사료의 품질을 비교하기 위하여 서울대학교 농과대학에서 사료의 일반성분 및 Ca, P의 분석은 물론 572수의 스타브로계 육욕종 병아리로 8주간 사양시험을 실시하였던 바 그 결과를 요약하면 다음과 같다. 1. 부로일러 사료의 가격은 제조하는 공장에 따라 전기의 경우 공장도 가격이 kg당 41.00에서 45.00원까지, 후기의 경우 공장도 가격이 kg당 39.00원으로부터 43.20원까지, 대리점 가격은 40.20원으로부터 47.20원까지로서 상당한 차이가 있었다. 2. 부로일러 사료의 일반 성분 및 Ca, P 함량에 있어서 제조하는 공장에 따라 상당한 차이가 있었는데 전기사료의 단백질함량은 20.38$\~$23.98$\%$, 후기의 것은 17.14$\~$21.57$\%$였으며, Ca, P함량은 앞으로 개선의 여지가 많았다. 3. 부로일러 사료의 제조공장에 따라 병아리의 성장율, 사료섭취량, 사료효율 등이 현저하게 달라졌다. 대체로 최종체중은 1.74$\~$1.91kg(평균 1.82kg)이었으며 사료효율은 2.4$\~$2.6정도였다. 4. 부로일러 사료의 품질이 다르고 또한 kg당 가격이 달랐기 때문에 조수익성에 있어서도 사료에 따라 크게 달랐다. 증체나 사료효율에 미치는 영향이 비슷한 경우 사료의 가격이 싼 것을 선택하는 것이 경제적으로 유리하다는 것이 밝혀졌다. 5. 앞으로 이와 같은 유형의 시험이 주기적으로 이루어져야 사료의 품질개선을 통한 양계업자의 권익보호가 될 것으로 생각되므로 정부는 현행 사료검사제도에 생물학적 검사를 겸행하기를 건의하는 바이다.

• Applied Biological Chemistry
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• v.18 no.4
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• pp.203-218
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• 1975

In order to investigate the compositional change oil composts during the growing of cultivated mushroom (Agaricus bisporus), composts and mushrooms during the period of filling to ending under commercial conditions were subjected to chemical analyses. The results are summarized as follows and the mechanism of composting for mushroom cultivation was proposed. 1) The temperature change of growing bed and room was observed and the yield of mushroom for each cropping time was recorded to get $15.6kg/m^2$ in total crops. 2) Composts after filling showed pH 8.2 which dropped to 6.4 after casing and continued so up to ending. 3) On the dry weight basis of composts, crude ash increased whereas total nitrogen, ether extract and crude fibre decreased gradually to bring about the lowering of organic matter. 4) Total nitrogen of composts decreased gradually and more insoluble nitrogen was lost than soluble nitrogen. The C/N ratio of composts was initially 21 which was gradually lowered to 16. 5) The losses of ${\alpha}-cellulose$, pentosan and lignin in composts were 87%, 75%, and 60%, respectively, in which ${\alpha}-cellulose$ decreased markedly after casing. 6) Free reducing sugars of composts increased continuously. Gradually increased free amino acids till second cropping decreased again thereafter. Composts at the filling stage contained alanine, glutamic acid, glycine and serine in which glycine decreased markedly whereas proline increased remarkably upon mushroom cultivation. 7) Among minerals of composts, phosphorus and zinc tended to decrease, potassium and copper tended to increase anti sodium showed no marked change. 8) In comparison of mushrooms from different cropping time with respect to proximate composition, minerals, free reducing sugars and amino acids, no marked difference was observed. However, a little higher values were observed in crude fat, free reducing sugars and sodium content for early crops and in free amino acids and phosphorus content for late crops. Twelve free amino acids including alanine, serine, threonine, and glutamic acid were detected in the cultivated mushroom. 9) According to above experimental results, it was possible to support the mechanism of compositing that the formation of ammonia and decomposition of carbohydrates by mesophiles are followed by protein biosynthesis, formation of microbial bodies and nitrogen-rich lignin humus complex by thermophiles, thus supplying necessary nutrients for mushroom growth, along with residual carbohydrates.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.3
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• pp.237-246
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• 1995

Larvae of the freshwater shrimp, Macrobrachium nipponense were reared in the laboratory at constant condition $(25^{\circ}C,\;7\%o)$, and their feeding rate, oxygen consumption rate, and growth rate were measured in regular intervals of time during larval development. Regression equations describing rates of feeding, growth and respiration as functions of time during individual larval molt cycles were inserted in a simulation model in order to analyse time-dependent patterns of variation as well as in bioenergetic efficiencies. Absolute values for feeding, growth, respiration and assimilation showed clear changes during the molt cycle, The absolute and specific values of respiration (R: R/C) showed small variation during the individual molt cycles. Significance of respiration in relation to growth (G) increased within the carbon budget, respiration rate (R/C) outbalanced growth rate (G/C) in late premolt. When the portion of metabolizable carbon is respired (R/G), metabolic coefficient was < 1 (i.e. R$(K_2)$ decreased concurrently, In cumulative carbon budget, total feeding was $491.54\;{\mu}g$ C/ind., assimilation was $85.3\%$, respiration was $47.7\%$, and growth was $37.6\%$ from hatching to postlarval stage.

• Journal of Life Science
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• v.21 no.11
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• pp.1573-1578
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• 2011

Milk thistle (silybum marianum) is a famous dietary supplement widely used in the United States and Europe. Silbinin is a major biologically active compound of milk thistle and has strong antioxidant and radical scavenger activities. Anticancer activities, as well as chemopreventive effects on various cancer cell lines, including prostate, lung, colon, skin, and bladder, have also been reported in silbinin. In the present study, we investigated the anticancer effects of silibinin and apoptosis through cell cycle arrest on prostate cancer cell PC-3. We performed cell viability by MTT assay and western blotting to confirm cell cycle check point proteins such as cyclin A/D1/E and cyclin-dependent kinase (CDK) 2/4/6. To quantify silibinin-induced apoptotic cell death of PC-3, Annexin V and PI double staining was performed by flow cytometry, by which its cell distribution was determined. As a result, silibinin inhibited the cell growth of PC-3 cells in a time- and dose-dependent manner, and its treatment resulted in cell cycle arrest at the G1 phase. Also the level of cell cycle check point proteins (cyclin, CDK) was decreased by silibinin in a dose-dependent manner. Taken together, we suggest that apoptosis of prostate cancer cell line PC-3 induced by silibinin is associated with cell cycle arrest through decrease of cell cycle check point proteins, caspase-3 activation and poly (ADP-ribose) polymerase (PARP) cleavage.