• Title/Summary/Keyword: 종 조합

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The Distribution and Feeding Guilds of the Polychaete Community in the West Coast off Kunsan, Korea (한국 서해 군새외해의 저서 다모류 분포와 섭식조합)

  • CHOI, JIN-WOO;KOH, CHUL-HWAN
    • 한국해양학회지
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    • v.27 no.3
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    • pp.197-209
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    • 1992
  • The distribution pattern of macrobenthic polychaete worms and their feeding guild structures were investigated along the coastal region off Kunsan (southeastern Yellow Sea) during the winter season. The polychaete worms, the most dominant infauna, comprised 54 species in 30 families and showed their mean density of 300. indir./m$^2$). Dominant species in the study area were Stermaspis scutata, Lumbrineris cruzensis, Notomastus sp., Nephtys polybranchia, Praxillella affinis. Polychaeres were assigned to 12 feeding guilds and BMX (burrowing, motile, muscular pharynx) was the most dominant feeding guild (26%). Muddy sediments sustained more polychaete worms which showed burrowing (65%), motile (72%), and non-tentaculate strategies (92%), while sandy sediments contained more filter feeders (32%) and sessile (63%), and more tentaculate worms (49%). The sediment grain size was suggested to be the prime factor selecting the polychaete feeding guild.

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Production and Characterization of Monoclonal Antibodies Specific to PAT Protein Expressed in Genetically Modified Herbicide-Resistance Maize (제초제 내성 유전자 변형 옥수수 중 PAT단백질에 특이한 단크론성 항체의 생산과 특성 확인)

  • Kim, Sol-A;Lee, Jeong-Eun;Shim, Won-Bo;Kang, Sung-Jo;Chung, Duck-Hwa
    • Journal of Food Hygiene and Safety
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    • v.33 no.3
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    • pp.193-199
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    • 2018
  • In this study, PAT protein of genetically modified maize was prepared from the recombinant E. coli strain BL21 (DE3), and mice were immunized with the recombinant PAT protein. After cell fusion and cloning, two hybridoma cells (PATmAb-7 and PATmAb-12) were chosen since the monoclonal antibodies (Mabs) produced by them were confirmed to be specific to PAT protein in the indirect enzyme-linked immunsorbent assay (ELISA) and western blot tests. There were no cross-reactions of either Mabs to other GM proteins or to the extracts of non-GM maize. The ELISA based on the PATmAb-7 can sensitively detect 0.3 ng/g PAT protein in corn. These results indicate that the developed Mabs can be used as bio-receptors in the development of immunosensors and biosensors for the rapid and simple detection of GM corn adulterated in foods.

Productivity and Performance Test of Egg-Type Commercial Korean Native Chickens (산란형 토종닭 실용계의 생산 및 산란 능력 검정)

  • Kang, Bo-Seok;Hong, Eui-Chul;Kim, Hak-Kyu;Kim, Chong-Dae;Heo, Kang-Nyeong;Choo, Hyo-Jun;Suh, Ok-Suk;HwangBo, Jong
    • Korean Journal of Poultry Science
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    • v.38 no.4
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    • pp.331-338
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    • 2011
  • The study was conducted to evaluate the production and the performance test of egg-type commercial Korean Native Chickens. A total of 360 female layers were produced from CY${\times}$C (A), CL${\times}$C (B), CG${\times}$C (C) and CW${\times}$C (D) crossbreds kept at National Institute of Animal Science, Korea, and breed during 20~64 weeks to investigate the productivity and the performance. Layers were divided into 4 crossbreds (6 replications/crossbred, 15 heads/replication). Age and egg weight at first egg was no significantly different among crossbreds. Body weight (BW) at first egg of D crossbred was significantly lower compared to that of other crossbreds (P<0.05). Weekly BW of B crossbred was the highest at 20, 30 and 40 weeks of age, but BW of D crossbred was the lowest among crossbreds. Feed intake (FI) of B crossbred was significantly higher compared to the other crossbreds at 20~24, 32~44 and 52~60 weeks of age. FI of D crossbred was significantly lower compared to other crossbreds after the age of 44 weeks (P<0.05). Average egg weight of B crossbred was significantly higher than the other crossbreds at the age of 24~60 weeks (P<0.05). Egg production ratio (%) of C crossbred was significantly higher compared to the other crossbreds for whole test periods (P<0.05). Hen-housed egg production number at 64 weeks of age for A, B, C and D crossbreds resulted in 241.4, 235.6, 232.3 and 227.0, respectively. Feed conversion ratio of A and C crossbreds was lower compared to the other crossbreds at the age of 20~24 weeks and C crossbred was the lowest among the crossbreds at the age of 60~64 weeks. These results provide the basic data which will be helpful to develope the new strains of commercial Korean Native Chickens.

Effect of Mixed Pasture Using Domestic Varieties Orchardgrass 'Kodione' and Tall fescue 'Purumi' on Forage Yields and Botanical Composition in Middle Region of Korea (중부지역에서 국내육성 목초 오차드그라스 '코디원' 및 톨 페스큐 '푸르미' 이용 초지조성 혼파조합별 사초생산성 및 식생에 미치는 영향)

  • Hwang, Tae-Young;Ji, Hee Chung;Kim, Ki Yong;Lee, Sang-Hoon;Lee, Ki-Won;Choi, Gi Jun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.2
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    • pp.89-97
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    • 2016
  • This study was carried out to determine the effect of mixed pasture using domestic varieties orchardgrass 'Kodione' and tall fescue 'Purumi' on forage yields and botanical composition from 2013 to 2015 in middle region of Korea. The following mixed pastures were used in this study: treatment A (orchardgrass oriented mixture with imported varieties), treatment B (orchardgrass oriented mixture), treatment C (orchardgrass and tall fescue oriented mixture), treatment D (tall fescue oriented mixture), treatment E (tall fescue oriented mixture without orchardgrass), treatment F (only orchardgrass), and treatment G (only tall fescue). In botanical compositions at last cutting time in 2015, treatment A had orchardgrass at 7.1%, tall fescue at 47.9%, kentucky bluegrass at 15.2% and white clover at 25%. Meanwhile, treatment B had orchardgrass at 25.9%, tall fescue at 47.0%, kentucky bluegrass at 4.9% and white clover at 18.3%. Consequentially, in botanical composition from 2014 to 2015, treatment A was changed more compare to other treatments B, C, D and E. The average of dry matter (DM) yield for 2 years of treatment E (18,369 kg/ha) was the highest among the seven treatments, but there was no significant difference among other treatments except treatment A (p>0.05). The crude protein (CP) and in vitro dry matter digestibility (IVDMD) of treatment A were 14.5% and 74.8%, and treatment E were 14.1% and 73.0%, respectively. The content of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were lower in treatment E and A than other treatments, and treatment E showed high content of total digestible nutrient (TDN). Therefore, tall fescue oriented mixture using domestic tall fescue variety Purumi had good forage productivity, quality and botanical composition in middle region of Korea for the establishment of grassland.

Detection of Fusarium verticillioides Contaminated in Corn Using a New Species-specific Primer (종 특이 primer를 이용한 옥수수 오염 Fusarium verticillioides의 PCR 검출)

  • Kang, Mi-Ran;Kim, Ji-Hye;Lee, Seung-Ho;Ryu, Jae-Gee;Lee, Theresa;Yun, Sung-Hwan
    • Research in Plant Disease
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    • v.17 no.3
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    • pp.369-375
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    • 2011
  • Fusarium verticillioides (teleomorph: Gibberella moniliformis), a member of the Gibberellea fujikuroi species complex, causes rots of corn stalks and ears, and produces a group of mycotoxins known as fumonisins that are harmful to animals and humans. Here, we focus on the development of a species-specific PCR primer set for differentiating F. verticillioides from other fumonisin-producing Fusarium species belonging to the species complex, such as F. proliferatum, F. fujikuroi, and F. subglutinans that are frequently associated with corn. The specific primers (RVERT1 and RVERT2) derived from the nucleotide sequences of RNA polymerase II beta subunit (RPB2) gene amplified a 208 bp-DNA fragment from only F. verticillioides isolates among the potential fumonisin-producing species examined; all of these isolates were shown to carry FUM1 required for fumonisin biosynthesis. The PCR detection limit using this specific primer set was approximately 0.125 pg/${\mu}l$ genomic DNA of F. verticillioides. In addition, the F. verticillioides-specfic fragment was successfully amplified from genomic DNAs of corn samples contaminated with Fusarium spp. This primer set would provide a useful tool for the detection and differentiation of potential fumonisin-producing F. verticillioides strains in cereal samples.

Genetic Analysis for Weight of Matured Silkworm and Number of Eggs Laid in Hybrid Population of the Silkwom, Bombyx mori (누에의 숙잠체중과 산란성에 대한 유전분석)

  • 정원복
    • Journal of Sericultural and Entomological Science
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    • v.35 no.2
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    • pp.100-104
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    • 1993
  • The genetics analysis for weight of matured silkworm(WMS) and number of eggs produced per moth(NEM) was studied by the seven parents diallel. Mean squares of additive effect, dominant effect, maternal effect and reciprocal effect were significant for two characters observed. The component of genetic variance analysis for WMS and NEM showed that dominant effect was higher than additive effect. Narrow sense heritability(h2ns) estimates were 0.773 and 0.228, in the WMS and NEM. The estimate of broad sense heritability(h2bs) value was higher than that of h2ns because of the low importance of dominance effect. Incomplete dominance was shown by Vr-Wr graphic analysis in the weight of matured silkworm and overdominance in the number of eggs produced per moth. In general combining ability effect, Jam 107 and Jam 124 was showed positively high for WMS and Jam 107 and S1 was expressed positively high for NEM. In specific combining ability effect, hybrids in S1XC51, S1XJam124 and Jam 107XJam 108 were exhibited positively high for WMS and Jam 107XN63, S1XC51, N74XJam 108 and Jam 107XJam 108 were found positively high for NEM.

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Genetic Analysis by Diallel Crosses in F1 Generation of Silkworm, Bombyx mori (이면교잡에 의한 가잠 F1세대의 몇 가지 양적 형질에 관한 유전 분석)

  • 정원복;장권열
    • Journal of Sericultural and Entomological Science
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    • v.28 no.1
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    • pp.24-29
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    • 1986
  • A set of 21 crosses of F1 generation by diallel crosses from seven parent silkworm, Bombyx mori L. were used as materials to estimate genetic parameters such as gene action and combining ability. Experiments were conducted in 1985, and three characters, which are weight of cocoon, weight of cocoon layer and ration of cocoon layer, were observed. The data were analyzed for each character by Jinks', Hayman's and Griffing's methods. The result obtained are summarized as follows : 1. In Vr-Wr graphical analyses, weight of cocoon was found to be inherited in over dominance, and weight of cocoon layer and ration of cocoon layer were observed as partial dominance. 2. Estimate of additive component of variance(D) was greater than those of dominance component of variance(H) for weight of cocoon layer and ratio of cocoon layer, and positive values of F were observed for all characters. 3. Mean squares of general combining ability were higher than those of specific combining ability. 4. Variety Jam 107 was expressed as the highest GCA effects for three characters, and high SCA effects for all characters were observed in combinations of 113 $\times$Jung 14 and Jam 107 $\times$Jung 14.

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Performance and Meat Quality of Three-Crossbreed Korean Native Chickens (KNC) (한국토종닭 3원교배종의 생산성과 육질 특성)

  • Park, Mi-Na;Hong, Eui-Chul;Kang, Bo-Seok;HwangBo, Jong;Kim, Hak-Kyu
    • Korean Journal of Poultry Science
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    • v.38 no.4
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    • pp.293-304
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    • 2011
  • The current work was carried out to investigate the effect of crossbred Korean native chickens (KNC) on performance and meat quality. A total of 720 chicks (1d of age) was used in this work and were divided into groups by crossbreds (A, B, C and D) and sex (male and female). Crossbreds were A) (KNC egg-meat type C strains ${\times}$ KNC meat type S strains) ${\times}$ Ross broiler, B) (KNC egg-meat type C strains ${\times}$ KNC meat type H strains) ${\times}$ KNC meat type S strains, C) (KNC native R strains${\times}$KNC meat type S strains)${\times}$KNC meat type H strains and D(White Semibroiler Chickens). Experimental diets consisted of 3 phases such as starter (0~5 weeks; CP 20.0%, ME 3,050 kcal/kg), earlier (5~8 weeks; CP 18.0%, ME 3,100 kcal/kg) and finisher (8~12 weeks; CP 16.0%, ME 3,150 kcal/kg). They were fed the broiler diets for 12 weeks at the flat house and thirty six chickens were slaughtered at week 5 and 10. There was no significant difference on the fertility of crossbred KNCs, and the hatchability of B crossbred was low compared to other crossbreds. On body weight (BW), D crossbred resulted in a higher BW after 5 weeks (P<0.05). Body weight gain (BWG) and feed intake (FI) of A crossbred were also significant higher compared to the other crossbreds for all periods. On carcass ratio (CR), A crossbred showed higher CR at 5 weeks, there was no significant among crossbreds at 10 weeks. The partial meat ratio(ala, breast, neck, leg) of C crossbred was the lowest among other crossbreds (P<0.05), but the back meat ratio was not difference among treatments at 5 and 10 weeks. pH of meats have no difference among crossbreds at 5 week, and A crossbred was high compared to other crossbreds at 10 week (P<0.05). The moisture content of D crossbred meat and the protein content of B crossbred meat were high compared to other crossbreds at 5 week. Chemical compositions of meats have no difference among crossbreds at 10 week. Lightness and redness have not significance among crossbreds at 5 week, and redness of A crossbred was the highest among all crossbreds (P<0.05). Shear force and cooking loss of A crossbred were high at 5 week, and cooking loss of A crossbred was low compared with other crossbreds at 10 week. These results suggested the basic data that needed to develope the new strains.

Core Technology of SOA: Semi-automatic Composition Techniques of Web Services (SOA의 핵심 기술: 반자동 웹 서비스 조합 기법)

  • Lee, Yong-Ju
    • Proceedings of the Korea Information Processing Society Conference
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    • 2008.05a
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    • pp.393-396
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    • 2008
  • 웹 서비스는 XML, SOAP, WSDL, UDDI, BPEL 등의 표준을 기본 구조로 하고 있으며 이러한 기술들은 SOA에서 요구되는 이기종 분산 플랫폼 상의 복잡한 애플리케이션 통합 문제를 유연하게 해결할 수 있는 다양한 인터페이스를 제공하고 있다. 본 논문에서는 SOA의 핵심 기술인 반자동 웹 서비스 조합 기법을 구현하기 위해 WS-BPEL 기법과 OWL-S 기법을 융합한 새로운 SemanticBPEL 조합 기법을 제안한다. 이 기법은 실험 분석을 통해 기존의 검색 방법보다 성능이 우수함을 보인다.

Development of a New Synthetic Korean Native Chicken Breed using the Diallel Cross-Mating Test (토종닭의 이면교배조합 시험을 이용한 신품종 종계 개발)

  • Sohn, Sea Hwan;Choi, Eun Sik;Kim, Ki Gon;Park, Byeongho;Choo, Hyo Jun;Heo, Jung Min;Oh, Ki Suk
    • Korean Journal of Poultry Science
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    • v.48 no.2
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    • pp.69-80
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    • 2021
  • We conducted a 4 × 4 diallel cross-mating test using 934 chickens from four grandparent stock lines to develop a new synthetic breed of Korean native chicken. The mean values, combining ability, and reciprocal effects on survival rate, body weight, and hen-day egg production were analyzed. In phenotypes, most chickens have yellowish-brown, reddish-brown and mixed color feathers. The average survival rate was 86.8±12.3%, with the highest in YH combination. Specific combining ability (SCA) had a greater effect on survival rate than general combining ability (GCA), and the SCA of HY combination was the highest. The 16 cross-combinations were distinctly divided into three weight groups according to their genetic characteristics. At 12 weeks of age, GCA showed a greater effect on weight than SCA, and the SCA of FH combination was the highest. The age at first egg laying was 157 days, and the crosses reached sexual maturity faster than the pure lines. The egg production rate was highest in SY at 79.5±2.1%. The GCA and SCA for hen-day egg production were similar, and the SCA was highest in the HS and FY combinations. The reciprocal effect showed that the offspring's egg production rate was high when S and Y were maternal parents in almost all combinations. In conclusion, FH and HF combinations, which have excellent growth performance, are the most desirable paternal parent stock strains, and FY, FS, HY, and SY combinations, which have excellent laying performance with moderate weight, are the preferred maternal strains.