• Current Research on Agriculture and Life Sciences
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• v.20
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• pp.9-18
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• 2002

A total of 31 C. chinense lines selected from 2000 screening were tested again for resistance to P. capsid but resistance was not found in tile lines. A total of 26 selections from Korean land races tested 2001 spring were tested again for resistance to P. capsici, KC180, KC230, KC195 and KC194 showed moderate resistance to P. capsid. However, it was apparent on the basis of horticultural characteristics that KC180 and KC230 had been naturally crossed with AC2258 and CM334, respectively. CM334 and AC2248 seed lots that were increased in different years were taken out and tested for resistance to improve their genetic purity because the resistant genetic resources have been showing some off-types in tile population. Off-types began to be found in 1992 seed lots and tile proportion and degree of tile offs was increasing with time up to 2001. Plants true to the type in 1992 seed lots were selected and their inbred seeds were mass produced in a net cage in the greenhouse. AC2258 included in the experiment together was uniform. In 1995 seed lots of CM334, plants with resistance to P. capsici and low or no number of lateral branching at cotyledonary axil, although they were off from tile original CM334, were found and selection was applied to breed lines fixed in tile characters.

• The KIPS Transactions:PartB
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• v.11B no.7 s.96
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• pp.841-848
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• 2004

Evolutionary a1gorithms are well-suited for multi-objective optimization problems involving several, often conflicting objectives. Pareto-based evolutionary algorithms, in particular, have shown better performance than other multi-objective evolutionary algorithms in comparison. However, generalized evolutionary multi-objective optimization algorithms have a weak point, in which the distribution of solutions are not uni-formly distributed onto Pareto optimal front. In this paper, we propose an evolutionary a1gorithm for multi-objective optimization which uses seed individuals in order to overcome weakness of algorithms Published. Seed individual means a solution which is not located in the crowded region on Pareto front. And the idea of our algorithm uses seed individuals for reproducing individuals for next generation. Thus, proposed a1go-rithm takes advantage of local searching effect because new individuals are produced near the seed individual with high probability, and is able to produce comparatively uniform distributed pareto optimal solutions. Simulation results on five testbed problems show that the proposed algo-rithm could produce uniform distributed solutions onto pareto optimal front, and is able to show better convergence compared to NSGA-II on all testbed problems except multi-modal problem.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.05a
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• pp.13-13
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• 2010

복주머니난(Cypripedium)속 식물은 우리나라에 광릉요강꽃을 비롯하여 털복주머니난, 흰털복주머니란, 복주머니란, 노랑복주머니란등 5종이 분포하는 것으로 알려져 있다. 광릉요강꽃과 털복주머니란 두 종은 환경부에서 지정한 멸종위기 식물 1급과 2급에 지정되어 보호를 받고 있고 나머지 3종은 제도적으로도 보호를 받지 못하는 실정이다. 본 연구에서는 충분히 성숙한 털복주머니난과 복주머니난의 종자에 NaOCl처리를 하여 발아율을 향상시킬수 있었는데 이러한 전처리가 발아에 미치는 원인에 대한 연구를 실시하였다. 털복주머니란의 무균적인 종자발아는 1.0% NaOCl 처리구에서 70% 이상의 종자발아율을 보였으며, POM배지가 MS배지보다 신초분화가 양호했다. GA3와 활성탄(Activated charcoal)의 혼합첨가는 신초증식에 효과적이었다. 신초분화 후 저온처리는 신초의 증식 율을 증가시켰다. 한편 NaOCl 농도(0, 1, 3, 5, 10%)와 NaOCl 처리시간(0, 5, 15, 30, 45, 60분)에 따라서 복주머니란의 종자발아를 확인한 결과, NaOCl 1%를 30분간 처리하였을 때 가장 높은 발아율을 나타냈다. NaOCl을 처리하여 종자의 종피상태를 SEM과 TEM으로 관찰한 결과 NaOCl의 처리는 종피 세포벽의 부분적 해리 및 작은 구멍을 만들게 하였는데 이러한 종피의 물리화학적 변화가 물과 양분의 이동을 원활히 하여 종자의 발아를 촉진하는 것으로 사료된다. 복주머니난의 신초분화에 미치는 casein과 활성탄의 효과를 알아본 결과 casein 200 mg/L와 활성탄 200 mg/L를 혼합 첨가한 실험구에서 가장 높은 신초분화율을 보였다. 토양순화 후 생존률은 극히 저조했으며 30 개체중에 5 개체만이 다음해 어린동아를 싹틔우는 것을 확인 하였다. 본 결과들을 종합하여 보면 멸종위기식물, 특히 털복주머니란과 복주머니란의 조직배양을 통해서 서식지외 보존방안(기내증식)에 관해 가능성을 제시하였다고 생각되어 진다.

• Horticultural Science & Technology
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• v.31 no.6
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• pp.756-763
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• 2013

Mechanical hair removal of carrot seed causes seed injuries and suppresses the germination in carrot cultivation. This study was performed to develop molecular markers for breeding high quality cultivars with short-hair seed. To meet this objective, random amplified polymorphic DNA (RAPD)-sequence characterized amplified region (SCAR) markers specifically linked to seed-hair characteristic were identified using CT-SMR 616 OP 389-1 line with short-haired seed and CT-SMR 616 OP 616-33 line with long-haired seed, bred by self-pollination for 6 years from 2008 to 2013, as parents. After seed hair lengths of these lines were analyzed using microscope, next generations were advanced and compared with the molecular markers polymorphism. From RAPD analysis using fixed lines in 2011, twelve RAPD primers showing polymorphic bands specific between the two lines were identified from 80 random primers. To develop RAPD-SACR marker, SCAR primers were designed based on sequence analysis of these specific RAPD bands and more than three combinations of primers were tested. As a result, it was found that the $SCA2_{1.2}$ amplified single polymorphic band from short-haired seed line. To confirm this result, $SCA2_{1.2}$ marker was retested by applying to the 2012 and 2013 progenies. Finally, it was concluded that the developed $SCA2_{1.2}$ marker distinguished short-haired line from long-haired seed line. Therefore, SCAR marker, $SCA2_{1.2}$ is expected to be utilized for breeding of the short-haired seed cultivars.

• Korean Journal of Plant Resources
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• v.15 no.2
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• pp.107-113
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• 2002

This study was performed to obtain the information on fruit set, propagation and flowering habits of the exotic ornamental bottle brush tree (Callistemon citrinus). Trees have fruit capsules for four years since the fruit set and they have enlarged for three years. While 2 and 3-year-old capsules have similar seeds in number and size, 1-year-old ones have fewer and smaller seeds. Most dried capsules open when they have 75％ relative water contents. Two year old seeds which they were dried at 30％ germinated better than others. Removal of capsule bearing twigs resulted in greatly increased subsequent flower numbers (an average of 14 flowers per tree) compared with twigs where seed capsule were allowed to remain. In cutting for propagation, semi-hard wood branches dipped in 100mgι$^{-1}$ IBA for 24 hours rooted at 86.4 ％ and had an average of 12.6 primary roots.

• Journal of Korean Society of Forest Science
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• v.34 no.1
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• pp.1-14
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• 1977

These studies were carried out to know the variation of morphological tree forms, needles, cotyledons, seed characters and top height growth of seedlings of Pinus koraiensis S. et Z. by populations in Mt. Jiri. The results are summarized as follows: 1. It was observed that the characteristics of length, needles of serration density of needles, percentage of full seeds length, of seeds, thickness, of seeds length of cotyledons, width of cotyledon, numbers of the stomata line in cotyledons and the top height growth of seedlings were significantly different in individual trees and populations. 2. The characteristics of branch angle specific gravity of wood and resin canal numbers of cotyledons were significantly different in populations. 3. It was that the characteristics of numbers of stomata line, width, of seeds weight, of seeds serration density of cotyledons, numbers of cotyledons were significantly different in individuals. 4. The resin canals of needles showed three principle resin canals in individual trees and population, and the coefficients of variation were not shown in individual trees and populations. Stem forms showed to be a straight form with all populations. 5. The characteristics of cotyledons had generally higher tendency than the characteristics of needles in comparison with coefficience of variation.

• Korean Journal of Agricultural and Forest Meteorology
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• v.12 no.3
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• pp.207-216
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• 2010

Individual differences of ozone ($O_3$) resistance for seed production, seed germination and seedling development were examined in this study. Five in each healthy and damaged trees of Pinus thunbergii growing in air polluted area for 12 years were chosen based on visible foliar injury and growth. The cones of P. thunbergii, which were collected from healthy and damaged trees, were analyzed for physical characteristics and seeds from the cones were used to test germination percentage under $O_3$ treatment. The germinated seeds were continuously exposed to $O_3$ treatment and the lipid peroxidation and activities of antioxidative enzymes were determined for both seeds and seedlings. The $O_3$ treatment for seed germination and seedling development were conducted at three conditions: control, 150 ppb and 300 ppb of $O_3$. The non-treated seeds from the damaged trees showed 21.6% lower germination than those from the healthy ones. On the $O_3$ treatment of 300 ppb, seed germination decreased approximately 10% for the healthy trees and 19% for the damaged trees compared to that on the control. The seeds from the healthy trees showed significantly higher activities of superoxide dismutase (SOD), glutathione reductase (GR), and catalase (CAT) than those from the damaged trees. The activities of GR, ascorbate peroxidase (APX), and CAT decreased along with the increasing $O_3$ concentration in two tree grades. Malondialdehyde (MDA) content of seeds was not influenced by $O_3$ treatment for two tree grades. In seedling development, there were no significant differences for length and biomass of needle and root of two tree grades at both the control and 150 ppb of $O_3$. At 300 ppb of $O_3$ treatment, however, the length and biomass of needle and stem decreased for two tree grades but no significant differences was detected in root. The seedlings from the damaged trees were more sensitive to the $O_3$ treatment, showing higher activities of SOD, APX, and CAT and content of MDA compared to those from the healthy tree seedlings. Our results indicate that seed germination and seedling development are vulnerable to increasing $O_3$ concentrations and that attention must be paid to the individual selection of tree species for reforestation.

• Journal of Life Science
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• v.18 no.7
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• pp.907-911
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• 2008

Radish (Raphanus sativus L.) is one of very important crop plants in the world. It is very important to determine hybrid seed quality in the production of hybrid Brassica vegetable seeds to avoid unacceptable contamination with self-inbred (sib) seeds. The allozyme for evaluating seed purity in a commercial $F_1-hybrid$ radish cultivar is demonstrated. Three hundred sixty seeds from the male and female harvest were subsequently screened for seed purity using 27 isozyme loci. Especially, F1 hybrids of radish, Per-1 ($aa{\times}bb$), Lap-1 ($aa{\times}bb$), Est-1 ($aa{\times}bb$) were presented clear hybrid bands. Est-1 locus revealed that 15 (8.3%) seeds from the female harvest and 26 (14.4%) seeds from the male harvest were sibs. It maintains higher than average level of genetic diversity compared with their correspondent parents. Shannon's index of phenotypic diversity (I) of hybrids was the highest of all accessions (R. sativus L. cv. Daepeng, R. sativus L. cv. Backza, and their hybrids). The allozyme may lead to a better insight into the hybrid seed purity.

• Journal of The Korean Society of Grassland and Forage Science
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• v.4 no.3
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• pp.194-200
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• 1984

Effects of seed coating on establishment, early growth and herbage production were investigated in Pot, Soil box and Field experiments. Seed was coated with 60% Lime, 20% Phosphate and 20% Peatmoss(W/W), also finely sprayed 13% Arabic gum and 2% Methyl cellulose(W/V). Coating were generally 1:20, seed: coat(W/W). 1. In the general growth of the growing stages and different oversown species at 80 days, seed coated was promoted than none in early growth. (Table 2 and 3) 2. Stand counts at 60 days after oversown, seed coated was higher 15% than none, especially, there was advantage due to coating on the medium and large size seeds such as Orchardgrass, Perennial ryegrass and Kentucky bluegrass, but did not assist establishment of the large (Tall fescue) and small (Ladino clover) size seed. 3. Variation of sward composition was not quite different between two treatments, but seed coated was slightly increased the Percentage of sown grasses with advance of cutting time. 4. Total dry metter yield per unit area with seed coated was higher 17% than none in establishment year. 5. Variations in the chemical composition of the herbage and soil were influenced by coating materials in the coating regime (Table 8). 6. From these facts, seed coated also appeared that adequate seedling stands, early growth and herbage production could be obtained with higher than conventional overseeding method under a poor conditions.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.10-10
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• 2023

투구꽃속(Aconitum L.)은 미나리아재비과(Ranunculaceae)의 가장 큰 속(Genus) 중 하나로 300-400 여종(Species)이 북반구 온대지역에 분포한다(Tamura, 1993). 둥근 투구모양의 꽃받침이 특징적이며, aconitine과 같은 알칼로이드 성분을 함유하고 있어 전통적으로 약용소재로서 활용되었다(Khorana, 1968). 세뿔투구꽃(Aconitum austrokoreense Koidz.)은 한국 경상도, 전라남도에만 국지적으로 자라는 한국 특산식물이자, IUCN에서 CR(Critical Endangered) 등급으로 지정된 희귀식물이다. 이 종은 다년생이지만, 서식지에서 개체수가 적거나 유묘의 발견이 어려운 등 자생지 축소 및 절멸의 우려가 높다. 세뿔투구꽃을 보호하고 종을 보존하기 위해서는 종자 발아 · 휴면에 대한 지속적 연구와 종자번식법의 개발이 필요하다. 본 연구에서는 세뿔투구꽃의 종자의 휴면유형을 분석하고 호르몬에 의해 휴면타파를 유도하였으며, 특히 발아 2단계에서 일어나는 생리적 변화를 단백질체학적 관점에서 분석하였다. 세뿔투구꽃 종자는 미발달된 배를 가지고 있으며, 충분한 수분과 온도의 조건에서도 발아가 관찰되지 않아 형태 · 생리적 휴면으로 판단되었다. 종자는 BAP 처리에서 휴면이 타파되는 것을 확인하였으며, 특히 BAP 농도에 따른 발아율은 통계적으로 유의한 값을 가졌다 (F=23.208, P<0.01). 건조된 종자에 비해 BAP 처리된 종자는 발아 2단계에서 몇가지 단백질의 증가가 관찰되었다. 대부분의 종자는 30S ribosomal protein subunit으로, 휴면상태에서 발아상태로 변환이 일루어지는 생리적 변화의 증거로 확인된다. 특히 발아2단계를 지나는 세뿔투구꽃 종자에서는 cytochrome subunit과 photosystem II protein의 급격한 증가가 관찰되었는데, 이는 배아의 발달 결과인 자엽이 발아 즉시 광합성이 가능하도록 준비하는 과정에서 발현 된 것으로 추정한다. 본 연구는 발아2단계에서 휴면타파에 직접적인 영향을 미치는 요인을 발견하지 못하였으나, 휴면타파에 중요한 단계인 발아 2단계 종자의 생리학적 변화를 이해하기 위한 기초자료로 활용 될 수 있을 것이다.