• Title/Summary/Keyword: 종자 개체

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Hibiscus syriacus 'Dasom', A New Flower-Color Mutant Variety Developed by Radiation Breeding (방사선 육종에 의한 화색변이 무궁화 신품종 '다솜' 육성)

  • Kim, Sang Hoon;Kim, Dong Sub;Kim, Jin-Baek;Ha, Bo-Keun;Lee, Duk Man;Song, Hi Sup;Kang, Si-Yong
    • Horticultural Science & Technology
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    • v.33 no.2
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    • pp.298-301
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    • 2015
  • A new Hibiscus syriacus variety, 'Dasom', was developed by radiation breeding using gamma ray exposure at the Korea Atomic Energy Research Institute (KAERI). Seeds of the original variety, 'Hongsun', were collected in 1993. These seeds were irradiated at 100 Gy dose and $M_1$ seeds were planted in a field of the KAERI in 1994. Lines with preferred flower color that were going to be used for street trees were initially selected in 1998. Analyses of characteristics were conducted from 2005 to 2010. The 5-year-old 'Dasom' plants were approximately 106 cm in height and averaged 19 branches, and thus were suitable for street trees. The flowers of 'Dasom' were semi-double type with medium red color and red eye spots. Petal length and width were about 4.0 cm and 3.3 cm, respectively. Flower diameter was slightly small, at about 5.8 cm, because of highly overlapping petals. The flowering period of 'Dasom' was about 105 days, which was 6 days shorter than that of the original variety, 'Hongsun'. However, the main charactersistics of 'Dasom' including leaf shape were similar to those of the original variety, 'Hongsun'.

Selection of Heat-tolerant Potato Clones by In Vitro Tuberization from True Potato Seeds (감자 眞正種子의 器內小塊莖 形成에 의한 耐暑性 系統 選拔)

  • 김현준;김영화;유승렬;김병현;김정간
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.2
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    • pp.77-81
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    • 1997
  • For the early selection of heat-tolerant clones, the true potato seeds of 750 clones were obtained by several cross combinations : $CIP\;575015\;{\times}\;katahdin,\;CIP\;575015\;{\times}\;B6603-6,\;84\;I\;35-4\;{\times}\;katahdin,\;CIP\;575015\;{\times}\;NookSack,\;and\;CIP575015\;{\times}\;Superior.$ The ratios of in vitro tuberization at 3$0^{\circ}C$ were decreased by 43% in all cross combinations compared with at 2$0^{\circ}C$. In particular, tuberization rate of $CIP575015\;{\times}\;katahdin$ cross at 3$0^{\circ}C$ was only 21%. On the other hand, the rate of tuberization of $CIP575015\;{\times}B6603-6$ cross was 58 % at 3$0^{\circ}C$, so this cross combination was thought to be good heat-tolerant clone. To screen the heat-tolerant clones in lower land during high temperature period, microtubers were cultivated in Kangnung, and the characters of tubers and productivity were examined. Among screened clones, one clone at 2$0^{\circ}C$ and three clones at 3$0^{\circ}C$ were shown to be heat-tolerant. The yield of 89ML75-8 was 88% more than that of Superior, and dry weight rate of 89 ML64-11 was 18.8%. Therefore, 89ML64-11 clone was considered as a good cultivar for processing.

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Production of Transgenic Melon from the Cultures of Cotyledonary-Node Explant Using Agrobacterium-Mediated Transformation (Agrobacterium 공동 배양을 통한 자엽절 절편 배양으로부터 멜론 형질전환체 생산)

  • Cho Mi-Ae;Song Yun-Mi;Park Yun-Ok;Ko Suck-Min;Min Sung-Ran;Liu Jang-Ryol;Lee Jun-Haeng;Choi Pil-Son
    • Journal of Plant Biotechnology
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    • v.32 no.4
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    • pp.257-262
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    • 2005
  • Agrobacterium tumefaciens-mediated cotyledonary-node explants transformation was used to produce transgenic melon. Cotyledonary-node explants of melon (Cucumis melo L. cv. Super VIP) were co-cultivated with Agrobacterium strains (LBA4404, GV3101, EHA101) containing the binary vector (pPTN289) carrying with CaMV 35S promoter-gus gene as reporter gene and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selective agent, and the binary vector (pPTN290) carrying with Ubiquitin promoter-GUS gene and NOS promoter-nptll gene conferring resistance to paromomycin as selective agent, respectively. The maximum transformation efficiency (0.12%) was only obtained from the cotyledonary-node explants co-cultivated with EHA101 strain (pPTN289) on selection medium with 5 mg/L glufosinate and not produced a transgenic melon from the cotyledon or cotyledonary-node co-cultivated with other strains. Finally, five plants transformed showed the resistance in glufosinate antibiotic and the GUS positive response in leaf ($T_0$), flower ($T_0$), seeds ($T_1$) and plantlet ($T_1$). Southern blot analysis revealed that the gus gene integrated into each genome of transgenic melon.

A Standard Rose Cultivar 'Love Letter' with Thornless Stems and Red Colored Petals for Cut Flowers (무가시성 적색 절화장미 '러브레터' 육성)

  • Lee, Young Soon;Jung, Yun Kyung;Park, Mi Ok;Lim, Jae Wook
    • Horticultural Science & Technology
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    • v.32 no.2
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    • pp.269-275
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    • 2014
  • A standard rose cultivar, 'Love Letter' was selected for cut flower from the progenies of a cross between 'Red Giant' and 'Ensemble' by the rose breeding team of the Gyeonggi-do Agricultural Research & Extension Services (GARES) in 2011. A standard rose cultivar 'Red Giant' with red colored (RHS Red Group 45C) petals and 1.8 prickles per stems of 10 cm was used as a mother plant. A standard rose cultivar 'Ensemble' with white and red purple colored (RHS White Group 155C + RHS Red Group N57B) petals and 2.4 prickles per stems of 10 cm was used as a pollen parent. 'Love Letter' was crossed in 2007 and seedlings were produced. After tests of specific characters from 2008 to 2011, this cultivar was finally selected and named. As a standard type with large sized flower, it has red colored (RHS Red Group 46A) petals with 9.3 cm flower diameter and 32.4 petals per flower. Vase life of this cultivar could be as long as 12 days. It takes 43 days from pruning to blooming and cut flower productivity was 152 stems/$m^2$ in a year. The stems of cut flower have no thorn and the length is about with 70.5 cm. When this cultivar grew below $15^{\circ}C$ and 1,000 lux, the petals color became dark red in winter season. 'Love Letter' was registered as a new cultivar on Korea Seed & Variety Service (KSVS) with No. 4482 on May 8, 2013.

Effects of Different Water Depths on Emergence of Barnyardgrass, Echinochloa crus-galli P. Beauv. (피의 발생(發生)에 미치는 담수심(湛水深)의 영향(影響))

  • Kang, Byeung-Hoa
    • Korean Journal of Weed Science
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    • v.6 no.1
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    • pp.7-12
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    • 1986
  • This experiment was conducted to investigate effects of different water depths on emergence, emerged soil depth and growth of E. crus-galli in Seoul, Korea. Plant number of emergence was decreased with deepened water depth, and dry weight of plant was especially negatively affected. However, a few E. crus-galli were emerged under 24 cm water depth. Soil depth of germination was increased as water depth decreased, and a few E. crus-galli were emerged below 10 cm soil depth under - 12 ㎝ water depth. But most E. crus-galli were emerged in ca. 2 cm soil depth under water depth over 0 cm.

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Effects of Artificial Acin Rain on Seed Germination, Growth and Needle of Several Conifers(2) (인공산성우가 몇 침엽수종의 종자발아, 생장 및 침엽에 미치는 영향(2))

  • 김갑태;추갑철
    • Korean Journal of Environment and Ecology
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    • v.7 no.1
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    • pp.10-21
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    • 1993
  • Artificial acid rain (pH 3.0, 4.0 and 5.0) and ground was treated on the seeded pots of 4 species to examine its effects on germination and survival rate, seedling growth and contact angles on needle surface. Artificial acid rain was prepared by diluting sulfuric acid with ground water and ground water (pH 6.5) was used as control. Artificial acid rain was sprayed to seeded pots two or three times per week for growing season, one times per week for winter seasons. About 5mm of artificial acid rain was treated each time from early April, 1991 to early October, 1993. Germination and survival rate, soil acidity, seedling growth and contact angles on the needle surface were measured and compared among the treatments. The results were summarized as follows ; Artificial acid rain might have positive effects on growth-related characteristics of the seedlings in the first and second year of acid rain treatment, and the effects were differed among four species. All growth-related characteristics of the seedlings in third year, however, decreased with decrease of pH values of artificial acid rain. This was considered to the results of acidic accumulation over soil buffer capacity. Needle injury and biomass (defoliation) was correlated with the pH values of artificial acid rain, and this character might be a good criteria for early diagnosis of acid rain injury. The differences of soil acidity were significant among the treatments for all species. Contact angles between needle surface and water droplet decreased with decrease of pH values of artificial acid rain. Measuring and comparing contact angles might be very good criteria for early diagnosis of acid rain injury.

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Studies on the Root Formation in Hypocotyl Cuttings of Maples (단풍나무류(類)의 배축삽수(胚軸揷穗) 발근(發根)에 관(關)한 연구(硏究))

  • Choi, Man Bong
    • Journal of Korean Society of Forest Science
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    • v.42 no.1
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    • pp.67-73
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    • 1979
  • In order to observe the anatomical phenomena of root formation in the hypocotyl cuttings of maples, the hypocotyls of Acer palmatum. Acer micro-sieboldianum, and Acer saccharinum were used as the materials. The rooted portions were sectioned by a microtom and doubly stained by safranin and fast green. The results obtained are summarized as follows; 1. A cross section structure of hypocotyl cuttings of Acer sp. is consist of epidermis, cortex, vascular bundle and pith from the outside. The vascular bundle makes circular shape forming polyarch in A. saccharinum and tetrarch in both A. palmatum and A. micro-sieboldianum. 2. An adventitious root of A. saccharinum originated in phloem parenchyma, while that of A. palmatum and A. micro-sieboldianum originated in interfascicular parenchyma related with phloem cells. 3. The hypocotyl cuttings of Acer sp. is commonly composed of parenchyma tissue having vigorous differentiation capability. Therefore, the originated root grow easily through the cortex and epidermis breaking their tissues.

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Effect of BA Concentrations and Culture Methods on in Vitro Plant Multiplication from Shoot-Tip Culture of Wasabia japonica (고추냉이 정단배양에 있어서 BA 농도 및 배양방법에 따른 기내증식 효과)

  • Park, Yun-Young;Cho, Moon-Soo;Lee, Young-Deuk;Chung, Jong-Bae;Park, Shin;Jeong, Byeong-Ryong;Park, Sang-Gyu
    • Journal of Plant Biotechnology
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    • v.34 no.1
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    • pp.1-6
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    • 2007
  • Effect of BA concentrations and culture methods on in vitro plant multiplication from shoot-tip cultures of Wasabia japonica was studied. Shoot-tips with leaf primordia and apical meristem were cultured on MS basal medium for all the experiments. Liquid medium for 2 weeks followed by semi-solid medium for 4 weeks containing 1.0 mg/L BA was the best to number of shoots (22.8) and shoot length (3.5 cm). Shoots proliferated could be divided into ca. 5 to 11 of cultures for the multiplication of plantlets. Divided plantlets showed root formation (90%) well onto MS basal medium without growth regulators like IBA and NAA. After rooting, all the plantlets transferred into the pots containing composed soil (bio-media Co., peatmoss $8{\sim}10%$, coir dust $66{\sim}70%$, zeolite $13{\sim}17%$, vermiculite $3{\sim}7%$, perlite $2{\sim}4%$) and grown well into whole plants with multiple shoots.

Survey on the Farms in Main Producing Area of Job's Tears (율무 주산 지역 농가의 실태조사)

  • 강치훈;박기준;유창재;김두환
    • Korean Journal of Plant Resources
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    • v.14 no.1
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    • pp.77-83
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    • 2001
  • This survey was carried out to collect the basic informations for the improvement of cultivation in job's tears (Coix lachryma-jobi L.) by investigating the general facts, farming practices, growth characteristics, grain yield, and chemical properties of soil in Yonchon-gun farms. The age of farmers were over 51 and the cultivated area was small. Most farmers were self-laboring and produced seeds on their land. Sowing method was usually drilling by hand, 64% of farms had the density of 2000-3500 plant per 10 a, amount of fertilizer application was diverse and the number of pest control was a few. The averages of plant height, number of main culm node, culm diameter, tiller number of plant, thousand grain weight, and grain yield were 184 cm, 10, 10 mm, 14, 108 g, and 341 kg/10a, respectively. The averages of pH, organic matter, $P_2O_5$, K, and EC of soil after harvest were 5.8, 1.1 g/kg, 385 mg/kg, 0.48 cmol+/kg, and 0.21 dS/m, respectively. Grain yield and hardness were negatively correlated -0.7442 at the level of P=0.01.

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Crossability and Chromosome Variation in the Early Generation of the Crosses between the Hexaploid Triticale and Diploid Rye (6배체 트리티케일 2배체 호밀과의 잡종 초기세대에서 교잡 친화성 및 염색체 변이)

  • 황종진;이홍석;하용웅
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.6
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    • pp.485-495
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    • 1991
  • This experiment was carried out to obtain the information on the crossability, variation of chromosome number in pollen mother cell (PMC) and somatic cell of the progeny from the cross between hexaploid triticale cv. Sinkihomil and two diploid rye varieties. Seed set was 39.3 to 41.6% (averaged 40.5%) in the cross between triticale (P$_1$) and rye(P$_2$), which resulted in 0.33% in F$_2$(selfed F$_1$), 2.69% in F$_1$/P$_1$ 5.47% in F$_1$/P$_2$ respectively. However, seed set was extremely low in both reciprocal crosses when triticale was used as male. Germination rate of the crossed seed was 94.0% in F$_1$ 40.8% in F$_2$(selfed F$_1$), 59.5% in F$_1$/P$_1$ and 65.9% in F$_1$/P$_2$ from the cross between triticale and rye, respectively. Pollen fertility of F$_1$ plant was averaged 18.7% in the cross between triticale and rye. Number of Uni-, Bi-, and Trivalent in PMC was 12. 6, 6.94, and 0.53, respectively, in the F$_1$ between the triticale and rye. There were 28 chromosomes in F$_1$, 21 to 34 in F$_2$, 34 to 38 in F$_1$/P$_1$ and 19 to 23 in F$_1$/P$_2$ from the cross between the triticale and rye, respectively.

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