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Analysis of Chromosome aberrations by fluorescence in situ hybridization using triple chromosome-specific probes in human lymphocyte exposed to radiation (3중 DNA probe를 이용한 FISH(fluorescence in situ hybridization) 기법으로 방사선에 의한 염색체 이상 분석)

  • Chung, Hai-Won;Kim, Su-Young;Ha, Sung-Whan
    • Journal of Radiation Protection and Research
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    • v.24 no.1
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    • pp.45-53
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    • 1999
  • Fluorescence in situ hybridization with chromosome-specific probe has been shown to be a valid and rapid method for detection of chromosome rearrangements induced by radiation. This method is useful for quantifying structural aberrations, expecially for stable ones, such as translocation and insertion, which are difficult to detect with conventional method in human lymphocyte. In order to apply FISH method for high dose biological dosimetry, chromosomal abberations by radiation at doses of 1, 3, 5, and 7Gy were analysed with whole chromosome-specific probes by human chromosome 1, 2 and 4 according to PAINT system. The frequencies of stable translocation per cell equivalent were 0.04, 0.33, 1.22, 2.62, and 5.58 for the lymphocyte exposed to 0, 1, 3, 5, and 7Gy, respectively, and those of dicentric were 0.00, 0.06, 0.52, 1.19 and 2.44, respectively. Significantly more translocation of t(Ab), a translocated chromosome with a piece of painted acentric matrial 'b' attached to unpainted piece containing centromere 'A', than reciprocal chromosome t(Ba) was observed. The frequencies of all type of chromosome rearrangements increased with dose. From above result, FISH seemed to be useful for radiation biodosimetry by which the frequencies of various types of stable aberrations in human lymphocyte can be observed more easily than by conventional method and so will improve our ability to perform meaningful biodosimetry.

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Effect of Dietary Microalgae, Diatom-Dominant, Oil Extracts on Growth, Body Composition and Shell Color of Juvenile Abalone Haliotis discus (배합사료내 규조류 우점인 미세조류 오일 추출물 첨가가 까막전복(Haliotis discus)의 성장, 체조성 및 패각 색채에 미치는 영향)

  • Kim, Hee Sung;Lee, Ki Wook;Jeong, Hae Seung;Kim, June;Yun, Ahyeong;Cho, Sung Hwoan;Lee, Gye-An;Kim, Keun-Yong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.6
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    • pp.738-744
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    • 2017
  • Effect of dietary inclusion of microalgae, diatom-dominant, oil extracts (MOE) on growth, body composition and shell color of juvenile abalone Haliotis discus was investigated. One thousand four hundred and seventy juvenile abalone were distributed into 21 plastic rectangular containers. Seven experimental diets were prepared: MOE0, MOE0.01, MOE0.05, MOE0.1, MOE0.5, MOE1 and MOE2 diets containing MOE at the concentrations of 0, 0.01, 0.05, 0.1, 0.5, 1 and 2% at the expense of mixture of squid liver and soybean oils, respectively. The experimental diets were fed to abalone in triplicate once a day with a little leftover for 16 weeks. Weight gain and specific growth rate of abalone fed the MOE1 and MOE2 diets were higher than those of abalone fed the all other diets. The shell length and soft body weight of abalone fed the MOE2 diet were longer and heavier than those of abalone fed the all other diets. Crude protein and ash content of the soft body of abalone were affected by dietary inclusion of MOE. The shell color of abalone fed the all experimental diets was different from that of wild abalone. In conclusion, dietary inclusion of MOE improved growth of abalone, but did not shell color of abalone.

Effects of Dietary Inclusion of Soybean Meal and Fermented Soybean Meal on Growth and Body Composition of Juvenile Abalone Haliotis discus (Reeve 1846) (배합사료내 대두박 및 발효대두박 첨가가 까막전복(Haliotis discus) 치패의 성장과 체조성에 미치는 영향)

  • Kim, Hee Sung;Jeong, Hae Seung;Choi, Dong Gyui;Jang, Bok-Il;Kim, Hyeon Jong;Lee, Ki Wook;Cho, Sung Hwoan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.6
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    • pp.812-817
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    • 2017
  • This study evaluated the effects of dietary inclusion of soybean (SM) and fermented soybean meal (FSM) on growth promotion in juvenile abalone Haliotis discus. Six hundred and thirty juvenile abalone were distributed into nine plastic containers. Three diets were prepared with 22% fish meal, 2% casein, and either 25% SM or FSM used as the protein sources in the experimental diets, and Undaria was used as a control diet to compare the effects of the experimental diets on the growth performance of abalone. The diets were assigned to three containers each and fed to the abalone once daily to satiation for 16 weeks. Weight gain and specific growth rate (SGR) were higher in abalone fed the SM diet than in those fed the FSM diet. Weight gain and SGR of abalone fed the SM and FSM diets were higher than those of abalone fed Undaria. The crude protein and lipid contents of the edible portions of abalone fed the SM and FSM diets were higher than those of abalone fed Undaria. SM was superior to FSM as a protein source in abalone feed. The SM and FSM diets facilitated greater growth than Undaria.

Quantitative Analysis of Effects for Quality Control on Medical Primary Class LCD Display Devices Based on AAPM TG18 Report (AAPM TG18에 의한 진단용 LCD 디스플레이 장치 정도관리 효과의 정량적 분석)

  • Jung Hai-Jo;Kim Hee-Joung
    • Progress in Medical Physics
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    • v.17 no.2
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    • pp.77-82
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    • 2006
  • The image display is an Important component of PACS and of medical digital imaging chain. Displayed image qualify is affected by the physical characteristics of display device, appropriate clinical settings and calibrations, and ambient lighting conditions. The performance of display systems is continuously degraded over time due to luminance deterioration and changes of clinical setting parameters. A routine QC is recommended because the performance of display systems is continuously degraded over time. Ten flat panel monochrome LCD display devices were included in the evaluation of the QC effect. The effect of QC on primary class LCD medical display devices for selected QC tests was evaluated by comparing the performances, luminance response, luminance dependencies, display resolution and display chromaticity in this study, of before and after the calibration procedures. The effects of the QC are significant to luminance response and luminance spatial dependencies test and the other side, are slight to the display resolution and display chromaticity test. A routine QC of display device is essential for the consistency of medical image display and presentation. The study of the QC effects of display devices will play an important role in practical QC procedures of display devices.

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A Study on Diagnostic Criteria of Noise-Induced Hearing Loss among Workers in an Iron Foundry (철강공장 근로자를 대상으로 살펴본 소음성 난청 진단기준에 관한 조사)

  • Kim, Ji-Yong;Lim, Hyun-Sul;Cheong, Hae-Kwan;Moon, Ok-Ryun
    • Journal of Preventive Medicine and Public Health
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    • v.26 no.3 s.43
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    • pp.371-386
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    • 1993
  • This study was carried out to evaluate diagnostic criteria of noise-induced hearing loss (NIHL) among-workers in an iron foundry. Of 1,093 workers under the observation of noise-specific health examination, 184 workers were selected by way of first and second screening audiometric examination. A questionnaire survey, otological examinations, Rinne test and audiometric test were performed and the results were as follows ; The degree of hearing impairment in the left ear was more severe than in the right ear (p<0.05). The difference between hearing threshold of the first and the second hearing test at 1,000 Hz was about 5 dB with a narrow range of deviations while the difference at 4,000 Hz was about -7 dB with a wide range. Of the total study workers, 84.8% were tested within 15 hours away from noise exposure, and the rest after 16 hours. This study has identified that mean hearing loss at 4,000 Hz showed a significant statistical difference among the two study groups while mean hearing loss by 4-divided classification did not. The same phenomena were observed between the group with and without tinnitus and between the group with and without difficulty in hearing (p<0.05). Among 184 workers, 10 workers (5.4%) diagnosed as NIHL by old diagnostic criteria in contrast to 150 workers diagnosed as NIHL by the new diagnostic criteria. There was a significant difference between the two groups in the average hearing loss at 4,000 Hz and 4-divided classification (p<0.01), but there were no significant differences in age, the duration of employment, blood pressure and the duration wearing the personal hearing protector (p>0.05). If we apply Early Loss Index (ELI) method, some workers in younger age group diagnosed as NIHL by the new diagnostic criteria were fallen into within the normal range. In the mean time older age group show reverse results in contrast to the above finding. It is too early to confirm the value of the usage of the new diagnostic criteria in hearing examination. Further study is called for to verify the value of this criteria.

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Thaumatin Isolated from Katemfe Fruit of Thaumatococcus daiellii Inhibits 3T3 L1 Adipocytes Differenciation (Thaumatococcus daiellii 열매 유래 토마틴의 3T3-L1 지방전구세포 분화 억제에 의한 항비만 효과)

  • Cha, Jae-Young;Jeong, Jae-Jun;Yang, Hyun-Ju;Park, Jun-Seok;Kim, Hyun-Woo;Kim, Su-Hyun;Jung, Hae-Jung
    • Journal of Life Science
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    • v.21 no.6
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    • pp.783-787
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    • 2011
  • The effects of thaumatin isolated from katemfe fruit of Thaumatococcus daiellii Benth on 3T3-L1 preadipocyte differentiation was investigated in vitro. 3T3-L1 adipocytes were treated with various concentrations of thaumatin ranging in 0-5 ${\mu}M$. Thaumatin reduced fat accumulation in differentiated 3T3-L1 adipocytes in a dose-dependent manner. 3T3-L1 cell proliferation was 97.0 and 88.3% at 1 and 3 ${\mu}M$ after 8 days of thaumatin treatment, respectively. Thaumatin showed a potent inhibitory effect on stained lipid droplets at a concentration of 3 ${\mu}M$. Thaumatin reduced triglyceride accumulation in differentiated 3T3-L1 cells in a dose-dependent manner, compared with positive control cells. This study provides basic information on the anti-obesity activity of thaumatin.

Development of a Quality of Life Scale For Children on Chronic Peritoneal Dialysis (만성 복막투석 아동용 삶의 질 척도 개발)

  • Shin, Min-Sup;Cho, Soo-Churl;Jang, Jae-Yeon;Cheong, Hae-Il;Choi, Yong;Ha, Il-Soo
    • Childhood Kidney Diseases
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    • v.12 no.2
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    • pp.202-212
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    • 2008
  • Purpose : The aim of this study was to develop a quality of life scale for children on chronic peritoneal dialysis(QOL-CPD). Methods : Thirty children on chronic PD at Seoul National University Children's Hospital participated. A healthy control group included 47 elementary school children. Other patients groups are 32 children from the department of pediatric orthopedics and 28 children from the department of child psychiatry. The age range of all children was 7 to 16 years. Preliminary items of the QOL-CPD were developed and administered along with the Korean version of the Children's Depression Inventory(CDI) to all children. Results : The final QOL-CPD was constructed by excluding those items with a factor loading of less than .20, and the principal axis factor analysis was performed again. The QOLCPD demonstrated a good internal consistency with a value of .87. The dialysis and childpsychiatric groups showed significantly lower QOL scores compared to the healthy control group. In addition, the dialysis and child-psychiatric groups showed greater difficulties on physical and academic functions. For the CDI, the PD group showed a mild level of depression. Conclusion : The results of this study demonstrate the clinical utility of a newly developed self-report QOL scale specific for children on chronic PD.

A study on the Mineral Contents of Korea Common Foods and Analytical Methods. 2. Potassium (한국인의 상용식품 중 무기질 함량과 분석방법 비교 연구 2. 칼륨)

  • 송범호;황성희;이주돈;김희재;정해랑;문현경
    • Journal of Food Hygiene and Safety
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    • v.7 no.1
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    • pp.37-44
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    • 1992
  • In order to observe the K contents, Korean common foods, especially processed foods were analyzed by Atomic Absorption Spectrophotometer. The results obtained were summarized as followed; (I) The K contents of instant noodle(ramyon) was 105-180 mg/100g and K ontents of their soup powder was 500-1000 mg/100g. (2) The K contents of potato products and bean products were less than those of original foods. So it was recongnized that the K contents might reduced during the food process. (3) The K contents of meats was 130-475 mg/I00g and those of meats products was relatively low, 80-260 mg/100g. (4) The K contents of carbonate beverage was very low but that of instant coffed was very high, 2400-2600 mg/100g. (5) There was no Statistical difference between the results of wet ash method and dry ash method in the K contents of all food groups.

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Physicochemical Composition and Antioxidative Effects of Yacon (Polymnia Sonchifolia) (야콘의 이화학적 성분과 항산화 효과)

  • Kim, Ah-Ra;Lee, Jae-Joon;Jung, Hae-Ok;Lee, Myung-Yul
    • Journal of Life Science
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    • v.20 no.1
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    • pp.40-48
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    • 2010
  • This study was carried out to investigate the physicochemical and functional properties of Yacon (Polymnia Sonchifolia) powder. The proximate composition of Yacon powder as a dry matter basis was 3.53% moisture content, 1.13% crude protein, 0.40% crude fat, 0.79% crude ash, 1.63% dietary fiber and 92.52% carbohydrate. The major free sugars were identified as fructose and glucose. Analysing total amino acids, 18 kinds of components were isolated from Yacon powder. The essential amino acid contained in Yacon powder accounted for 28.40% of total amino acid, while the non-essential amino acid accounted for 73.61%. Analysing total fatty acids, only 2 kinds - palmitic acid and lauric acid - were detected. Oxalic acid was the major organic acid. The contents of vitamin A, vitamin C and vitamin E were 0.057 mg%, 0.670 mg% and 0.001 mg%, respectively. The mineral contents of Yacon powder were in the order of Zn

A Gender-Sensitive Approach on Home Economics in Secondary Education (중등 가정과 교육의 성격에 관한 성인지적 접근)

  • 정해숙
    • Journal of Korean Home Economics Education Association
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    • v.15 no.2
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    • pp.55-66
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    • 2003
  • In Korea home economics in the secondary school level was only taught to girls until 1995. So. home economics education only for girls was criticized to sexist education by the feminists. According to the 6th revision of curriculum. home economics has been taught to girls and boys. But some of curriculum developers raises another questions on home economics education. They argue that home economics is a subject which prepares students for the demand of their day-to-day lives. but they can manage their lives well without school learning. The purpose of this study is to explore the educational values of home economics in secondary education from the gender-sensitive approach. For this study, a literature survey on the nature of home economic education in secondary education and the content analysis on 12 secondary school textbooks of Home Economics were employed. The results are as follows : First, the educational values of home economics education should not be underestimated. The aims of home economics education is to help students grew into a independent person by managing their daily lives. The home is charged with fostering a side of our life which we might call Personal, private. intimate and nurturing. The management of the home needs good skills and attitudes. and many knowledges on the child rearing, family relation, marriage, love and sex, which can not be successfully acquired without school learning. And home economics education for boys and girls can help break down the barriers between the sexes because it can change the attitudes of students regarding the private sphere. which is traditionally where women have worked. Second. most of contents of Home Economics were composed of the women-oriented contents. There was a trend still describing that domestic labor mainly be carried out by mother. And the ideas that only the family consisting of two parents and their children is normal were founded. Finally directions of development of the Home Economics Education are suggested.

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