Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Thaumatin Isolated from Katemfe Fruit of Thaumatococcus daiellii Inhibits 3T3 L1 Adipocytes Differenciation

Thaumatococcus daiellii 열매 유래 토마틴의 3T3-L1 지방전구세포 분화 억제에 의한 항비만 효과

  • Cha, Jae-Young (Technical Research Institute, Daesun Distilling Co., Ltd.) ;
  • Jeong, Jae-Jun (Technical Research Institute, Daesun Distilling Co., Ltd.) ;
  • Yang, Hyun-Ju (Technical Research Institute, Daesun Distilling Co., Ltd.) ;
  • Park, Jun-Seok (Technical Research Institute, Daesun Distilling Co., Ltd.) ;
  • Kim, Hyun-Woo (Technical Research Institute, Daesun Distilling Co., Ltd.) ;
  • Kim, Su-Hyun (Technical Research Institute, Daesun Distilling Co., Ltd.) ;
  • Jung, Hae-Jung (Technical Research Institute, Daesun Distilling Co., Ltd.)
  • 차재영 (대선주조(주) 기술연구소) ;
  • 정재준 (대선주조(주) 기술연구소) ;
  • 양현주 (대선주조(주) 기술연구소) ;
  • 박준석 (대선주조(주) 기술연구소) ;
  • 김현우 (대선주조(주) 기술연구소) ;
  • 김수현 (대선주조(주) 기술연구소) ;
  • 정해정 (대선주조(주) 기술연구소)
  • Received : 2011.06.03
  • Accepted : 2011.06.10
  • Published : 2011.06.30
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Abstract

The effects of thaumatin isolated from katemfe fruit of Thaumatococcus daiellii Benth on 3T3-L1 preadipocyte differentiation was investigated in vitro. 3T3-L1 adipocytes were treated with various concentrations of thaumatin ranging in 0-5 ${\mu}M$. Thaumatin reduced fat accumulation in differentiated 3T3-L1 adipocytes in a dose-dependent manner. 3T3-L1 cell proliferation was 97.0 and 88.3% at 1 and 3 ${\mu}M$ after 8 days of thaumatin treatment, respectively. Thaumatin showed a potent inhibitory effect on stained lipid droplets at a concentration of 3 ${\mu}M$. Thaumatin reduced triglyceride accumulation in differentiated 3T3-L1 cells in a dose-dependent manner, compared with positive control cells. This study provides basic information on the anti-obesity activity of thaumatin.

3T3-L1 지방전구세포 분화 억제에 대한 Thaumatococcus daiellii 열매 유래 토마틴의 항비만 효과를 검토하였다. 3T3-L1 지방전구세포에 토마틴을 0-5 ${\mu}M$ 농도로 처리한 결과 세포생존율은 처리 농도 의존적으로 감소하였는데, 토마틴 8일 처리 후 1 및 3 ${\mu}M$ 농도에서 각각 97% 및 88.3%의 세포생존율을 보였다. 또한 토마틴 3 ${\mu}M$ 처리 농도에서 Oil-Red-O염색 지방구가 현저히 감소된 것으로 나타났다. 3T3-L1 세포 내 중성지방 농도는 양성 대조군에 비해 농도 의존적으로 감소하였다. 따라서 천연 식물성 토마틴은 3T3-L1 지방전구세포의 세포증식 억제 및 중성지방 농도 감소 효과를 보여 항비만 효과가 있는 것으로 밝혀졌다.

Keywords

References

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