• Radiation Oncology Journal
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• v.21 no.1
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• pp.54-65
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• 2003

Purpose : To analyze the gene expression Profiles of uterine ceulcal cancer, and its variation after radiation therapy, with or without concurrent chemotherapy, using a CDNA microarray. Materials and Methods :Sixteen patients, 8 with squamous ceil carcinomas of the uterine cervix, who were treated with radiation alone, and the other 8 treated w14h concurrent chemo-radiation, were Included in the study. Before the starling of the treatment, tumor biopsies were carried out, and the second time biopsies were peformed after a radiation dose of 16.2$\~$27 Gy. Three normal cervix tissues were used as a control group. The microarray experiments were peformed with 5 groups of the total RNAs extracted individually and then admixed as control, pre-radiation therapy alone, during-radiation therapy alone, pre-chemoradiation therapy, and during-chemoradlation therapy. The 33P-iabeled CDNAS were synthesized from the total RNAs of each group, by reverse transcription, and then they were hybridized to the CDNA microarray membrane. The gene expression of each microarrays was captured by the intensity of each spot produced by the radioactive isotopes. The pixels per spot were counted with an Arrayguage, and were exported to Microsoft Excel The data were normalized by the Z transformation, and the comparisons were peformed on the Z-ratio values calculated. Results : The expressions of 15 genes, including integrin linked kinase (ILK), CDC28 protein kinase 2, Spry 2, and ERK 3, were increased with the Z-ratio values of over 2.0 for the cervix cancer tissues compared to those for the normal controls. Those genes were involved In cell growth and proliferation, cell cycle control, or signal transduction. The expressions of the other 6 genes, Including G protein coupled receptor kinase 5, were decreased with the Z-ratio values of below -2.0. After the radiation thorapy, most of the genes, with a previously Increase expressions, represented the decreased expression profiles, and the genes, with the Z-ratio values of over 2.0, were cyclic nucleotlde gated channel and 3 Expressed sequence tags (EST). In the concurrent chemo-radiation group, the genes involved in cell growth and proliferation, cell cycle control, and signal transduction were shown to have increased expressions compared to the radiation therapy alone group. The expressions of genes involved in anglogenesis (angiopoietln-2), immune reactions (formyl peptide receptor-iike 1), and DNA repair (CAMP phosphodiesterase) were increased, however, the expression of gene involved In apoptosls (death associated protein kinase) was decreased. Conclusion : The different kinds of genes involved in the development and progression of cervical cancer were identified with the CDNA microarray, and the proposed theory is that the proliferation signal stalls with ILK, and is amplified with Spry 2 and MAPK signaling, and the cellular mitoses are Increased with the increased expression oi Cdc 2 and cell division kinases. After the radiation therapy, the expression profiles demonstrated 4he evidence of the decreased cancer cell proliferation. There was no sigificant difference in the morphological findings of cell death between the radiation therapy aione and the chemo-radiation groups In the second time biopsy specimen, however, the gene expression profiles were markedly different, and the mechanism at the molecular level needs further study.

• Journal of the Korean Geographical Society
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• v.49 no.5
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• pp.728-746
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• 2014

The purpose of this study is to analyze the influence of the selective migration of young age group and the stage migration which has proceeded through generations, on the transition of fertility and the population growth in the rural and urban in the case of Jeollabuk-do. For this, we use O-D matrix of 20-34 age group, the distribution of that group and women of child bearing age, and vital statistics in 1970-2010. The major findings of this study are as follows. First, the outflows from their birth place are common and dominant features of 20-34 age group in each time. Second, there is the stage migration which preceding generation moved from the rural areas to the cities in Jeollabuk-do and following generation has moved from the cities to Seoul metropolitan area. Third the selective outflow migration of young age group has leaded to reduction of the childbearing population, declining birthrate, aging and natural population decline. Due to the stage migration, these depopulation processes occurred in rural area in the past, and currently it expands to the cities with about 15 years time gap. In fact, there have been the natural population decrease which annual number of deaths exceed that of births from the late 1980s in the most rural areas and in the early 2000s, such a phenomenon has been confirmed also in urban areas. Therefore, this study concludes that the stage migration through generations is one of the crucial factor to the population growth in local cities and also brings out the step-wise population decrease in settlement hierarchies.

• Korean Journal of Veterinary Research
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• v.49 no.3
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• pp.257-263
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• 2009

Bovine mastitis is an important disease causing serious economic loss in dairy production and food poison in public health. Escherichia coli and Staphylococcus aureus are the major causative agents of bovine mastitis. These bacteria were found in milk and environmental condition such as feces, water, soil and so on. Bovine mastitis causative micro-organisms can survive in 1-2 weeks in feces and bed complexes. Low level of percentage of water content (PWC) of feces and bed complexes can reduce the spreading of bovine mastitis incidence from environmental contamination. In this study, we developed the fecal dehydrating system and determined the elimination rates of bovine mastitis causative agent from feces and bed complexes. To develop the fecal dehydrating system, the screw pressurized dehydrating system was used and the maximum rate of dehydrating was reached to 52% PWC using 90% PWC (wet base) of fecal and bed complexes. The elimination rates of the dehydrating system for E. coli and S. aureus were reached at 41.19 $\pm$ 7.84% to 62.55 $\pm$ 8.71% in various percentages of PWC of feces and bed complexes (80, 85 and 90%). These results suggested that the application of fecal dehydrating system would reduce the exposure of dairy cattle to bovine mastitis causing agents contaminated feces and bed complexes, and can be used for environmental bovine mastitis control avoiding misuse or abuse of chemical disinfectants and antibiotics in dairy farm.

• Clinical and Experimental Pediatrics
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• v.49 no.9
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• pp.983-986
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• 2006

Purpose : The purpose of this study was to investigate the presence of Bartonella henselae DNA, which is known as an etiologic agent of lymphadenitis, in fleas from dogs. Methods : The Bartonella henselae infection was investigated in 42 fleas from 22 dogs in Korea. By using seminested PCR targeting pap31 gene, B. henselae DNA was amplified from fleas. Results : B. henselae DNA was detected in seven fleas (7 of 42 fleas, 16.7 percent) from four dogs (4 of 22 dogs, 18.2 percent). To confirm these findings, we performed sequencing and identified the seven PCR products. Sequence analysis revealed that six sequences belonged to Huston-1 genogroup and one sequence to Marseille genogroup. Conclusion : These results may suggest that dogs could be an important source of B. henselae infection in children in Korea. This is the first report about the detection of B. henselae in fleas from dogs in Korea.

• Korean Journal of Materials Research
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• v.3 no.6
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• pp.632-637
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• 1993

The optical properties and electrochromism of amorphous $WO_3$ thin films were studied. $WO_3$ thin films with thickness of 3000$\AA$~6000$\AA$ were deposited by vacuum evaporat.ion. All these films were transparent and found to be amorphous in structure by X-ray diffraction analysis and the visible wave length refractive indices were found to be between 1.9 and 2.1 and the optical energey gap to be 3.25 eV. Electrochromic devices were made consisting of IT0 transparent electrode, $WO_3$ thin films, $LiCIO_4$- propylene carbonate and Pt counter electrode. In terms of their operation, the amorphous $WO_3$ films were colored blue by a double injection of electrons from the transparent electrode and lithium ions from the $LiCIO_4$-propylene carbonate organic electrolyte and made colorless by electrochemical oxidation reaction. The electrochromic properties of $WO_3$ thin films including coloration and bleaching, optical density and response time were all found to be strongly dependent on the film deposition condition, electrolyte concentration, sheet resistance of the transparent electrode and applied voltage.

• Journal of Pharmaceutical Investigation
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• v.38 no.4
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• pp.261-267
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• 2008

The aim of the present study was to evaluate the bioequivalence of two gabapentin preparations. We used Neurontin tablet 800 mg (Pfizer Korea Inc.) as a reference drug for bioequivalence of Gabalep tablet 800 mg (Chong Kun Dang Pharmaceutical Co., Korea), and performed this whole study according to the guidelines of Korea Food and Drug Administration (KFDA). Twenty five healthy male volunteers were administered with each drug in a randomized $2{\times}2$ cross-over study with one week washout interval. After drug administration, blood was taken at predetermined time intervals ($0{\sim}24$ hours) and the concentrations of gabapentin in serum were determined using an high performance liquid chromatography-tandem mass spectrometer (LC-MS/MS) employing electrospray ionization technique and operating in multiple reaction mornitoring (MRM). The analytical method was validated in specificity, accuracy, precision and linearity. The phar-macokinetic parameters such as AUCt and Cmax were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUCt and Cmax. $Mean{\pm}SD$. of AUCt and Cmax value for reference drug and test drug were $29.94{\pm}9.23\;({\mu}g/mL{\cdot}hr)$ and $3.12{\pm}1.11\;({\mu}g/mL{\cdot}hr)$, and $31.48{\pm}9.77\;({\mu}g/mL{\cdot}hr)$ and $3.15{\pm}1.03\;({\mu}g/mL)$, respectively. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.25) for AUCt and Cmax, respectively. These results indicate that Gabalep tablet 800 mg is bioequivalent to Neurontin tablet 800 mg.

• Journal of the Korea Concrete Institute
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• v.24 no.4
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• pp.391-398
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• 2012

This paper presents the shear capacities of biaxial hollow slab with donut type hollow sphere. Recently, various types of slab systems which can reduce self-weight of slabs have been studied for increasing constructions of taller and larger building structures. A biaxial hollow slab system is widely known as one of the effective slab system, which can reduce self-weight of slab. According to previous studies, the hollow slab has low shear strength, compared to solid slab. Also, the shear capacities of biaxial hollow slab are influenced by the shapes and materials of hollow spheres. However, the current code does not provide a clear computation method for the shear strength of hollow slab. To verify the shear capacities of this hollow slab, one-way shear tests were performed. Four test specimens were used for test parameters. One was conventional RC slab and others were hollow slabs. The test parameters included two different shapes and materials of plastic balls. The shape parameters were donut and non-donut forms and the material parameters were general plastic and glass fiber plastic. The results showed that the shear strengths varied depending on hollow shapes and materials used in the slab.

• Journal of the Korean Society of Radiology
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• v.7 no.1
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• pp.9-15
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• 2013

In this study, texture feature analysis (TFA) algorithm to automatic recognition of liver disease suggests by utilizing computed tomography (CT), by applying the algorithm computer-aided diagnosis (CAD) of hepatocellular carcinoma (HCC) design. Proposed the performance of each algorithm was to comparison and evaluation. In the HCC image, set up region of analysis (ROA, window size was $40{\times}40$ pixels) and by calculating the figures for TFA algorithm of the six parameters (average gray level, average contrast, measure of smoothness, skewness, measure of uniformity, entropy) HCC recognition rate were calculated. As a result, TFA was found to be significant as a measure of HCC recognition rate. Measure of uniformity was the most recognition. Average contrast, measure of smoothness, and skewness were relatively high, and average gray level, entropy showed a relatively low recognition rate of the parameters. In this regard, showed high recognition algorithms (a maximum of 97.14%, a minimum of 82.86%) use the determining HCC imaging lesions and assist early diagnosis of clinic. If this use to therapy, the diagnostic efficiency of clinical early diagnosis better than before. Later, after add the effective and quantitative analysis, criteria research for generalized of disease recognition is needed to be considered.

• Journal of Acupuncture Research
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• v.31 no.2
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• pp.119-134
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• 2014

Objectives : The purpose of this study was to investigate the analgesic effects of filiform acupuncture and laser acupuncture at the points of $TE_3{\cdot}GB_{41}$ in neuropathic pain induced rats. Methods : Neuropathic pain in rats was induced by partial resection of the tibial and sural nerves. Three weeks after the neuropathic surgery, each of the experimental groups(AT, LAT, AT + LAT and LAT + AT) was injected at the $TE_3{\cdot}GB_{41}$ twice per week for three weeks. Results : 1. All of the experimental groups(AT, LAT, AT + LAT and LAT + AT) showed a significant decrease in the plantar withdrawal response of allodynia and the thermal allodynia as compared with the control group. During the early phase, the AT and AT + LAT groups have been marked as more significant than the LAT + AT and LAT groups. 2. The expression of c-Fos significantly decreased in the LAT and LAT + AT groups as compared with the control group. 3. The LAT + AT group showed a significant decrease in Bax as compared with the control group. In each experimental groups(AT, LAT, AT + LAT and LAT + AT), Bcl-2 increased and Bax/Bcl-2 ratio decreased as compared with the control group. 4. The LAT, AT + LAT and LAT + AT groups showed a significant increase in mGluR5 as compared with the control group. Conclusions : These results represented that the filiform acupuncture and laser acupuncture at the $TE_3{\cdot}GB_{41}$ exerted anti-apoptotic and neuroprotective effects on the model of neuropathic pain, thereby suggesting that they should be available for decreasing mechanical allodynia.

• Journal of Korean Ophthalmic Optics Society
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• v.19 no.2
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• pp.279-284
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• 2014

Purpose: This study will be a basic research providing data which can reduce eye strain using monitor and electric media by measuring legibility of text color on monitor. Methods: Total experimental subjects who don't have color anomaly and best corrected visual acuity was over 1.0 were 50 and their mean age was $22.83{\pm}1.47$. Reading speed test were carried out with 163 words and subjects who could read over 123 words were selected for legibility examination. Monitor background color was white and letter colors were black, blue, red, and yellow for the legibility examination. Results: In case of text color of black, blue, red, and yellow, Event duration(sec) was measured to 41.89, 42.89, 45.32, and 56.28, respevtively, and reading error (number) was measured to 1.94, 2.74, 3.36, and 5.14. Average of fixation duration(sec) was measured to 0.25, 0.26, 0.27, and 0.32. Conclusions: In this paper, event Duration, reading error and average of fixation duration which can measure the legibility was reduced in a sequence of black blue, red, and yellow.