• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
• /
• 2011.10a
• /
• pp.205-212
• /
• 2011

• Transactions of the Korean Society of Automotive Engineers
• /
• v.12 no.5
• /
• pp.94-100
• /
• 2004

In order to improve the frontal crash performance of an Aluminum Space Frame Vehicle, this presents a systematic optimal design process to maximize the crush energy absorption capacity of side-members while satisfying the maximum displacement constraint. In this study, five design types are studied for selecting a good collapse initiator. Then, for the selected collapse initiator type, 7 design variables are defined to represent cross section shape, thickness and bead interval. The systematic optimization processor, R-INOPL uses DOE, RSM and numerical optimization techniques. R-INOPL uses only 14 analyses to solve the 7 design variable optimization problem the final design can improve 103.9% of the internal energy and reduce 13.9% of the maximum displacement.

• KSBB Journal
• /
• v.13 no.2
• /
• pp.133-140
• /
• 1998

Using rlFN-$\alpha$ and rhGH as the model proteins, the refolding performances of the published processes were evaluated and compared. Key engineering parameters such as the type of denaturant and this concentration, protein concentration in the refolding buffer, and pH and ionic strength of the buffer were experimentally investigated. Furthermore, the role of a co-solvent of surfactant type in aggregation reduction was also studied. Of the denaturants tested (8M urea, 6M guanidine HCI, 0.5% SDS), SDS at alkaline pH (9.5) and ambient temperature gave the highest recovery yield. The SDS process was effective in the refolding of observed where dissolution proceeded better under lower strength (10 mM) but aggregation was suppressed under higher strength (>50 mM.) When PEG-4000 and/or Tween were added as co-solvent or refolding-enhancing additive, 1.6-2 times higher yield was realized. The‘masking’of the hyrophobic patches located on the surface of the protein with the surfactant molecules was believed to be responsible for the considerable reduction in aggregation during refolding.

• Proceedings of the Korean Society for Technology of Plasticity Conference
• /
• 2008.05a
• /
• pp.500-502
• /
• 2008

In generally, a high pressure fuel injection pipe has been often used as a fuel supply line in automobiles or other diesel engines. Such conventional high pressure fuel injection pipe, however, has suffered from the problem that is folding and hair cracks created therein. The defects can be locally formed in the inner wall surface of the pipe at the connecting head leading to a flow path when the pipe is deformed by the heading process. In the study, in order to prevent the folding in the inner wall surface of the pipe during the heading process, FE-analysis has been used in the die design.

• Proceedings of the Korean Information Science Society Conference
• /
• 2001.10a
• /
• pp.595-597
• /
• 2001

오늘날 인간 유전체 프로젝트(Human Genome Project)의 완성은 인간의 모든 유전자 서열정보를 제공하게 되었으며, 이러한 데이터를 바탕으로 생명현상과 관련된 산업 및 연구가 각광받게 되었다. 특히 생명체의 특정 기능을 파악하기 위한 단백질 3차 구조에 대한 연구가 활발히 진행중이다. 본 논문에서는 단백질 3차 구조를 추상적으로 기술 할 수 있는 표현기법을 기술한다. 제안된 표현기법은 단백질 2차 구조요소($\alpha$-나선구조와 $\beta$-병풍구조)를 이용하여 인접한 구성요소간의 접힘(folding)에 대한 관계를 기술하여 추상적인 단백질 3차 구조를 표현한다. 제안된 표현기법으로 기술된 추상적 단백질 3차 구조 표현은 단백질 구조에 대한 보다 빠른 이해와 다른 단백질 구조와 비교될 수 있는 장점을 지닌다.

• Journal of the Korean earth science society
• /
• v.42 no.5
• /
• pp.479-495
• /
• 2021

This study investigates the synoptic (patterns of southern highs, northern lows, and lows rapidly developed by tropopause folding), thermodynamic, and kinematic characteristics of a strong wind that occurred in the Yeongdong region of South Korea on March 18-20, 2020. To do so, we analyzed data from an automatic weather station (AWS), weather charts, the European Centre for Medium-range Weather Forecasts (ECMWF) reanalysis, rawinsonde, and windprofiler radars. The daily maximum instantaneous wind speed, exceeding 20 m s^-1, was observed at five weather stations during the analysis period. The strongest instantaneous wind speed (27.7 m s^-1) appeared in the Daegwallyeong area. According to the analysis of weather charts, along with the arrangement of the north-south low-pressure line, the isobars were moved to the Yeongdong area. It showed a sine wave shape, and a strong wind developed owing to the strong pressure gradient. On March 19, in the northern part of the Korean Peninsula, with a drop in atmospheric pressure of 19 hPa or more within one day, a continuous strong wind was developed by the synoptic structure of the developing polar low. In the adiabatic chart observed in Bukgangneung, the altitude of the inversion layer was located at an altitude of approximately 1-3 km above the mountaintop, along with the maximum wind speed. We confirmed that this is consistent with the results of the vertical wind field analysis of the rawinsonde and windprofiler data. In particular, based on the thermodynamic and kinematic vertical analyses, we suggest that strong winds due to the vertical gradient of potential temperature in the lower layer and the development of potential vorticity due to tropopause folding play a significant role in the occurrence of strong winds in the Yeongdong region.

• Journal of the Korean Chemical Society
• /
• v.67 no.2
• /
• pp.81-88
• /
• 2023

The contribution of hydrophobic residues to the protein folding reaction was studied by using HubWA variant proteins with I and L to V mutation. Folding kinetics of all V variant proteins was observed to be satisfied by a three-state on-pathway mechanism, U ⇌ I ⇌ N, where U, I, and N represent unfolded, intermediate, and native state, respectively. Three-state folding reaction was quantitatively analyzed and the free energy of folding of each elementary reactions and overall folding reaction, ΔG^o_UI, ΔG^o_IN, and ΔG^o_UN, were obtained. From the ratio of free energy difference between the variant protein and HubWA, ΔΔG^o_UI/ΔΔG^o_UN (ΔΔG^o_UI = ΔG^o_UI (variant protein) - ΔG^o_UI (HubWA) and ΔΔG^o_UN = ΔG^o_UN (variant protein) - ΔG^o_UN(HubWA)), the contribution of hydrophobic residues to HubWA folding was analyzed. The residues which are located in the hydrophobic core between α-helix and β-sheet, I3, I13, L15, I30, L43, I61 and L67, showed ΔΔG^o_UI/ΔΔG^o_UN value of ~0.5 when each of these residues was mutated to V, indicating that these residues form relatively solid hydrophobic core in the intermediate state. Residues located at the end of secondary structures and loop, I23, L69 and I36 showed ΔΔG^o_UI/ΔΔG^o_UN value below 0.4 when each of these residues was mutated to V, indicating that the region containing these residues are loosely formed in the intermediate state. V17A, L50V and L56V showed fairly high ΔΔG^o_UI/ΔΔG^o_UN value of ~0.8. Since L50 and L56 are located in the region containing long loop (residue 46 to 62), it is suggested that the high ΔΔG^o_UI/ΔΔG^o_UN value of these residues prevents the formation of aggregate at the early stage of folding reaction.

• Journal of the Korean earth science society
• /
• v.34 no.7
• /
• pp.645-661
• /
• 2013

The synoptic, thermodynamic, and dynamic characteristics of a heavy snowfall event that occurred in Seoul metropolitan area on 27 to 28 December 2010 was investigated. During this period there was a distinctive case that was identified as a polar low. We analyzed surface and upper level weather charts, snowfall amount, sea surface temperature, satellite imagery, sounding, and the National Center for Environmental Prediction global $1^{\circ}{\times}1^{\circ}$ reanalysis data. The polar low developed in an area where there was strong baroclinicity in the lower level aided by strong conditional instability due to 925 hPa warm air advection and 700 hPa cold air advection. The development mechanism of polar low is due, in part, to the tropopause folding, which advected stratospheric air increasing potential vorticity in mid-level and inducing cyclonic vorticity and convergence in low-level. Eventually clouds developed and there were snowfall total of 10 cm in Seoul metropolitan area and as much as 20 cm in southern parts of Korea. During the snowfall development, there was a $-45^{\circ}C$ cold core at 500 hPa and shortwave maintained $3-5^{\circ}$ separation with surface trough, which favored the development of polar low located in the warm sector and cyclonic advection area. The height of the dynamical tropopause lowered to 700 hPa during the peak development and increase in potential vorticity allowed strong vertical motion to occur. Overall, there was a close relationship between the development of snowfall and tropopause undulation. The heaviest snowfall occurred east of the tropopause folding where strong cyclonic vorticity, vertical motion, and moisture advection all coincided while the polar low was passing through the Korean peninsula.

• Journal of Life Science
• /
• v.28 no.10
• /
• pp.1140-1146
• /
• 2018

Dual-specificity phosphatases (DUSPs) play a role in cell growth and differentiation by modulating mitogen-activated protein kinases. DUSPs are considered targets for drugs against cancers, diabetes, immune diseases, and neuronal diseases. Part of the DUSP family, DUSP19 modulates c-Jun N-terminal kinase activity and is involved in osteoarthritis pathogenesis. Here, we report screening of cavity-creating mutants and the crystal structure of a cavity-creating L75A mutant of DUSP19 which has significantly enhanced enzyme activity in comparison to the wild-type protein. The crystal structure reveals a well-formed cavity due to the absent Leu75 side chain and a rotation of the active site-bound sulfate ion. Despite the cavity creation, residues surrounding the cavity did not rearrange significantly. Instead, a tightened hydrophobic interaction by a remote tryptophan residue was observed, indicating that the protein folding of the L75A mutant is stabilized by global folding energy minimization, not by local rearrangements in the cavity region. Conformation of the rotated active site sulfate ion resembles that of the phosphor-tyrosine substrate, indicating that cavity creation induces an optimal active site conformation. The activity enhancement by an internal cavity and its structural information provide insight on allosteric modulation of DUSP19 activity and development of therapeutics.

• Journal of Fashion Business
• /
• v.24 no.5
• /
• pp.106-124
• /
• 2020

The purpose of this study was to analyze the three-dimensional (3D) characteristics and reproducibility of the effective expression of North American smocking pleats in the process of making clothes using a 3D virtual clothing system (CLO) and present a method of expression according to the types of North American smocking. In this study, lattice, lozenge, and flower smocking were produced as real smocking and 3D virtual content, and actual muslin properties were measured using a Fabric Kit and reflected using an emulator. The results of this study confirmed that a dense puckered design such as North American smocking could be expressed depending upon the internal line, fold angle, and reinforcement setting for 3D smocking. To partially apply pleats to flat fabrics, it was necessary to set fold lines. The fold line setting could be expressed by designing the internal line in horizontal, vertical, and diagonal directions according to the North American smocking design, and then setting the fold angle for each internal line. By setting fold angles of 0 degrees and 360 degrees according to the folding direction of the set internal line, the fabric was clearly folded and stable pleats were created. This study will contribute to the vitalization of the 3D virtual fashion content industry by analyzing and presenting the optimal expression method of sophisticated and complex pleats generated according to the North American smocking design pattern.