• The Korean Journal of Zoology
• /
• v.36 no.2
• /
• pp.159-174
• /
• 1993

까치 살모사(AEkistrodon snatilis)의 활동기와 동면기에서 일반적인 혈액성상과 혈액내 가스함량의 변화 또한 위장관 점액세포에 대한 형태적 및 조직화학적 변화 양상을 규명 비교하고자 한다 활동기와 동면기에 까치 살모사의 혈액내 총이산화탄소량(TCO2). 이산화탄소부분압(PCO2), 산소압(PO2)과 pH는 활동기보다 동면기에 증가하였으며, 적혈구수, 혈색소, 적혈구 용적 백분율은 활동기보다 동면기에 약간 증가하는 경향이었다. 또한 백혈구수는 활동기보다 동면기에 뚜렷한 감소를 보였다 활동기와 동면기의 위점막에서는 현저한 차이는 없으나, 소장 점막은 동면기에 그 주름수가 감소하고 높이가 낮아지며 점액세포가 위축되었으며, 위 표면 점액세포는 활동기에 중성 점액질과 약간의 Sialomucin을 포함한 산성 점액질로 구성되었으나 동면기에는 중성 점액질과 Sialomucin이 약간 감소하였다 위점액경세포는 활동기에 중성 점액질만을 포함하고 있었으나. 동면기에는Sialomucin을 포함한 산성 점액질이 소량 검출되었다 소장 점액세포는 활동기에 산성점액질과 중성 점액질로 구성되었으며. 산성 점액질에는 Sulfomucin과 Sialomucirr이 포함되어 있었으나 동면기에는 Sulfomucin과 Sialomucin이 다소 감소하는 경향이었다 주사전자현미경 관찰을 통한 까치 살모사의 위장관 표면 상피세포의 미세구조적 변화는 각동면기 별로 차이를 인정할 수가 있었다. 위장관점 막 상피세포는 활동기에 비해 동면기에 상당한 구조적 위축현상을 보이는 경향을 알 수 있다.

• Clinical and Experimental Pediatrics
• /
• v.46 no.1
• /
• pp.6-10
• /
• 2003

동물과 사람의 연구에서 위장관 영양 인자는 위장관 점막이 점막손상으로부터의 회복에 중요하고 출생 후 경구 영양에 적응할 수 있게 하는 데에 중요하다. 경구적으로든 전신적으로 투여된 성장 인자들은 위장관의 성장과 발달을 촉진시킨다. 신생아 혈중의 영양인자들이 장관 세포의 수용체를 통해 작용하여 위장관의 성장을 조율한다. 위장관 영양인자들은 체내에서 합성될 수도 있고 모유를 통해 공급된다. 사람에서 출생 후 위장관이 장관영양에 신속히 적응할 수 있도록 위장관 영양 인자들이 중요한 작용을 한다. 모유 내의 성장 인자들이 신생아 생존에 필수적인 것들은 않아도 모유를 먹은 영아들이 조제분유를 먹은 영아들에 비하여 급성 설사, 괴사성 장염, 크론씨 병과 같은 위장관 질환의 위험율이 낮다. 위장관 영양 인자들의 대부분이 시판 조제분유에는 존재하지 않고 주로 모유에 존재함을 앎으로써 모유의 장점을 설명하는 데에 적용할 수 있을 것이다. 그리고 위장관 영양인자는 위장관 점막 손상된 경우 치료 목적으로 사용될 수 있는 여지가 높다. 이러한 임상적 이용은 특히 미숙아, 수술 후의 영아 등에서 적용될 수 있다. 그러나 향후 더욱 연구되어야 할 항목들로는 작용기전, 경구 및 정맥 투여 방법에 의한 효과의 차이, 체내 성장 인자들과의 상호 작용, 외부적 투여가 체내 인자에 대한 영향, 위장관 이외의 타 기관에 대한 영향, 그리고 안전성과 약물 역동학적인 특성 등이다.

• Proceedings of the KOR-BRONCHOESO Conference
• /
• 1993.05a
• /
• pp.68-68
• /
• 1993

Immunohistochemistry combined with electron microscopy was employed to investigate the distribution of substance P -immunoreactive(SP-IR) nerve fibers in the tracheal submucosal gland of cats. SP-IR nerve fibers were found to form network around the glands. Numerous varicosities were also detected within the basement membrane of the acini and secretory tubules. All the intraglandular varicosities showed close spatial contact with serous cells, mucous cells and myoepithelial cells. Our findings suggest that SP-induced mucus secretion from tracheal submucosal glands in cats may be caused not only by glandular contractile response of myoepithelial cells but also by direct stimulation to both serous and mucous cells.

• Proceedings of the KOR-BRONCHOESO Conference
• /
• 1993.05a
• /
• pp.67-67
• /
• 1993

Mucosal surface of the trachea and larynx is protected by several complex defense mechanism, namely mucociliary clearance, immunoglobulin, antibacterial secretory enzyme, which have also been demonstrated in the middle ear mucosa and eustachian tube. The morphologgy of secretory glands and cells of the trachea and larynx is well-known, but knowledge concerning their development related to secretory activity is still sketchy. The secretory element of the murine trachea and larynx, aging from gestational day 16 to postnatal day 21, was studied using hematoxylin & eosin and alcian blue/periodic acid-Schiff staining including lysozyme immunohistochemistry to investigate the development of secretory element of the murine trachea and larynx and to provide with basis of the future study for developmental morphology of the trachea and larynx. The results of this study suggest that the secretory activity starts to be established immediately after birth with the aeration of the lung.

• The Korean Journal of Zoology
• /
• v.35 no.1
• /
• pp.45-57
• /
• 1992

생쥐를 48시간 동안 결식시킨 후 유문결찰하여 급성미란을 유발시키고 미란형성부의 점막 내위선을 구성하는 각 세포의 구조변화와 IgA 분비세포의 분포를 관찰하였다. 미란부 점액분포의 변화는 표면상피의 손상 정도가 심해질수록 경계부의 PAS양성반응은 감소되었으며 중심부에서는 표면상피의 소실로 인하여 PAS 양성반응이 관찰되지 않았다. Glycocalyx 관찰을 위한 ruthenium red 반응에서도 경계부의 상피세포 손상이 심해짐에 따라 정단세포막 상층에 나타나는 양성반응은 감소되었고 측면과 기저면의 양성반응은 관찰되지 않은 것으로 보아 세포 손상시 tight junction은 파괴되지 않음을 알 수 있었다. 미란경계부의 미세구조변화로서, 상피세포는 손상이 진행될수록 세포질이 용해되어 공포가 형성되고 핵응축 및 정 단세포질의 점액원과립의 소실이 관찰되었으나 tight junction은 유지되었다. 반면에 점액경세포는 점액원과립의 수가 증가되고 조면소포체가 종창되어 있었다. 미란의 경계부와 중심부에서 벽세포는 다수의 소포가 출현한 후 세포내세관이 종창되었으며 그 후에 mitochondria가 파괴되었고 주세포는 주변의 벽세포가 커지면서 가장자리로 밀려나 크기가 정상보다 작아지고 조면소포체와 mitochondria가 종창을 나타내었다. IgA분비세포는 점막이 파괴된 미란부 점막하조직의 혈관 주변에 다수 존재하는 형질세포에서 확인되었다.

• Journal of the Korean Applied Science and Technology
• /
• v.35 no.4
• /
• pp.1407-1412
• /
• 2018

This study suggests that ozonated olive oil can kill a wide variety of microorganisms while being less toxic but in the case of administration or abdominal administration, the human body is harmless. In Korea, there is no animal stability test yet. In this study, ozonated olive oil was used to test the stability of the animal through the eye. Specific experiments were performed to evaluate animal Ocular irritation safe test of ozonized oil (high concentration) in rabbits. and Observation of the treatment point on the test substance, ozonated olive oil showed no Ocular irritation in both the non-washing group and the washing group.

• Parasites, Hosts and Diseases
• /
• v.33 no.4
• /
• pp.313-322
• /
• 1995

Metosonimus yokosawai was found deeply invaded into the submucosa of the small intestine of mice (ICR) when they were immunosuppressed by prednisolone injection. Experimental groups consisted of control, fluke infection (1,800 metacercariae per mouse) and fluke infection plus immunosuppression. In fluke infection group, many worms were found sectioned in the intervillous space of the jejunum and ileum at 6 hrs, 12 hrs, and 1 day after infection, and pathological changes characterized by villous atrophy and crypt hyperplasia were observed. After 3 days, only a few worms were found in intestinal sections, and after 7 days, the pathological changes became minimal. No worm was found penetrated beyond the mucosal layer. On the other hand, in immunosuppressed mice, numerous worms were found sectioned in the duodenum and jejunum, irrespective of the infection period up to 14 days. Pathological changes of the mucosa were minimal until 3 days after infection, but at 5 days marked destruction of the mucosal layer was observed. At this time many flukes were found invaded deeply into the submucosa facing the muscular layer. Despite continuous immunosuppression, the mucosal damage was gradually recovered at 7-21 days post-infection. The results showed that immunosuppression of ICR mice can induce, for a short perid of time, severe mucosal damage, and allow deep invasion of M. yokogcuwai into the submucosa of the small intestine.

• Current Research on Agriculture and Life Sciences
• /
• v.1
• /
• pp.201-214
• /
• 1983

A study was undertaken on rats in order to clarify the gastric mucosal, morphologic response to oral and subcutaneous administration of acetylsalicylic acid(aspirin). Aspirin was administered orally (oral group) and subcutaneously (subcutaneous group) at does rate of 60 mg per kg of body weight per day to 40 normal rats. On the 1st, 3rd, 7th, and 15th day of administration, in addition to clinical observation, 5 rats each from the both groups were sacrificed and examined macroscopically, histologically and electron-microscopically for the morphological changes of gastric mucosal cells with the following results. Although the clinical, macroscopic and histological changes were not significant, marked ultrastructural changes were observed. Parietal and chief cells were affected most severely by the administration of aspirin ; parietal cells showed increase in the number of SER and intracellular canaliculi, where-as in chief cells fragmentation, luminal dilatation, decrease in the number and structural abnormalities of RER were seen. Relatively mild changes were observed in mucous, mucous neck and basal-granulated cells. Although the degree of changes was milder than those of oral group, the similar changes were also observed in the subcutaneous group. From these results, it would be concluded that aspirin injury of gastric mucosa is effected not only by the direct injury to the mucosa but also indirectly by the blood concentration of aspirin.

• Journal of Life Science
• /
• v.28 no.9
• /
• pp.1081-1091
• /
• 2018

The purpose of this study was to investigate the structure and the glycoconjugate properties of the olfactory mucosa in the rat after inhalation of xylene. Sprague-Dawley male rats were inhalated 300 ppm xylene for 5 times with 5 hours exposure. The olfactory mucosa in the nasal cavity was taken from the animals on 5, 10, 15, 20, and 30 days after inhalation of xylene. The properties of the glycoconjugates in the olfactory mucosa were investigated using 10 biotinylated lectins: (PSA, UEA I, PHA-L, GSL I B4, GSL I, PNA, ECL, SBA, GSL II, and sWGA). In the experimental groups, degenerative changes of the olfactory epithelium were observed until 20 days after inhalation of xylene. In the control group, the olfactory cells in the olfactory epithelium reacted with PSA, UEA I, PNA, SBA, and sWGA, the supporting cells reacted with PSA, PHA-L, GSL I, PNA, ECL, SBA, GSL II, and sWGA, and the Bowman's glands reacted with all 10 lectins. In the experimental groups, the reactivity to PSA, PNA, and SBA in the olfactory cells were decreased, and the reactivity to PSA, PNA, SBA, and GSL II in the supporting cells were decreased. And in the Bowman's gland, the reactivity to PSA, UEA I, GSL I, and sWGA were decreased. Conclusively, the olfactory mucosa was shown a lot of changes in the structure through degenerative process and in the properties of the glycoconjugates after inhalation of xylene. These results suggest that the sugar residues of the glycoconjugates in the olfactory mucosa can be changed by xylene inhalation.

• Applied Microscopy
• /
• v.6 no.1
• /
• pp.9-20
• /
• 1976

The present study was performed to clarify the histochemical compositions and fine structure of the mucus secreting cells in the gastrointestinal mucosa of normal mice. The mucus cells in the surface epithelium of stomach body had neutral mucin and some quantity of weak acid mucin. And the mucus cells in gastric pits and mucus neck cells had neutral mucin. The goblet cells in villial epithelium of small intestine contain strong acid sulfated mucin as their main content and a little of neutral mucopolysaccharide. However, the goblet cells in intestinal glands-Liberkuhn crypt were confirmed to contain non-sulfated weak acid mucin. The goblet cells in the surface epithelium of colon had the same component as the small intestine did. But the cells in the crypts of colon contained neutral and weak acid mucin as their main contents. The majority of secretory granules of the surface epithelial cells of the stomach body had high electron density, and some granules with low electron density appeared too. While the mucin granules in the mucus neck cells were low in its electron density, and some of those granules were frequently found to have dense core in them. Secretory granules in goblet cells of small and large intestines had low electron density. The mode of secretion in mucin-containing cells in gastro-intestinal tract was found to be merocrine.