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Pollen Tube Elongation and Fruit Set of Asian Pear (Pyrus pyrifolia) upon Mechanical Damage on the Style (동양배 암술의 기계적 손상에 따른 화분관 신장과 착과)

  • Kang, Sam-Seok;Kim, Yoon-Kyeong;Choi, Jang-Jeon;Cho, Kwang-Sik;Hwang, Hae-Sung;Jeong, Sang-Bouk;Lee, Hee-Jae
    • Horticultural Science & Technology
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    • v.28 no.6
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    • pp.942-947
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    • 2010
  • In the present study, pollen tube growth and fruit set in Asian pear ($Pyrus$ $pyrifolia$) were examined upon the mechanical damage on the stigma and style following the artificial pollination. When the upper middle part of style was cut off at 48 h after the artificial pollination, pollen tube grew to the middle of the style and the subsequent pollen tube elongation and fruit set normally occurred. When the style was cut off before the pollen tube reached the middle of the style, however, no pollen tube elongation and fruit set occurred. With the pollen tube elongation through the style, callose plugs at the basal part of the pollen tube were formed to the direction of the elongation at regular intervals. When the upper part of pollen tube attached pollen grain was cut off, however, callose plugs were formed defectively in the pollen tube, and the pollen tube opened and lost their turgor pressure. These facts might be the reason for the ceased or delayed pollen tube elongation and ultimately the failure of the fertilization.

An Experimental Study on the Engineering Properties of Lightweight Aggregate Concrete (경량골재 콘크리트의 공학적 성질에 관한 실험적 연구)

  • ;R. N. Swanmy
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.39 no.1
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    • pp.75-82
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    • 1997
  • 건설기술과 산업의 발전에 따라 구조물은 대형화되어 가고, 건설공사의 급격한 팽창으로 골재 수용량이 급증함에 따라 천연골재자원은 점차 부족현상을 면치 못할 처지에 있다. 또한, 무리한 천연골재의 채취는 자연환경을 훼손시킬 뿐만 아니라 자연보호 측면에서도 심각한 공해문제로 대두되고 있어 공급량 부족현상은 날로 심화되고 있다. 이에 세계 몇몇 나라에세는 산업부산물을 이용한 골재 생산으로 공해예방과 폐기물 활용방법을 연구하고 있다. 산업부산물중 플라이 애쉬 생산량은 전 세계적으로 매년 약 2억여톤에 달하고 있으나 이중 일부만 활용되고 있는 실정이다. 이와같은 부산물을 활용하기 위한 일환으로 산업부산물인 PFA(Pulverized Fuel Ash)로 만든 인공경량골재의 년생산량이 영국은 600,000$m^3$, 미국은 300,000$m^3$이며, 매년 증가주세에 있다. 고성능 경량골재 콘크리트는 단위중량의 증가없이 내구성과 강도를 향상시켜 실용화 측면에서 경제적인 효과가 있으며, 플라이 애쉬로 만든 경량골재는 시멘트와의 친화력이나 접착면에서 우수한 것으로 알려져 있다. 본 시험에 사용한 골재는 플라이 애쉬로 만든 인공경량 조골재와 강모래이고, 결합제로서 프틀랜드 시멘트를 사용하였다. 부수적인 결합재로서는 플라이 애쉬, 슬래그, 실리카 흄을 사용하였으며, 고성능 경량골재 콘크리트를 개발코자 재령 28일과 180일의 압축강도가 각각 50MPa와 60MPa가 되도록 배합설계를 하였다. 본 연구에서는 플라이 애쉬, 슬래그, 시리카 흄과 같은 산업부산물을 혼입했을때 경량골재 콘크리트의 압축강도, 휨강도, 동탄성계수, 공극체적, 공극률, 단위중량, 공극 크기별 분포등의 변화를 실험적으로 구명하여 재반 구조용 콘크리트에 활용하기 위한 기초자료를 마련코저 한다.있어 특정한 발육단계의 난포 사망기전을 연구하기 어렵다. 또한 난포는 생체 내에서 다양한 호르몬을 동시에 분비하기 때문에 특정한 난소국부호르몬이 사망기전에 미치는 영향을 조사하기 힘든 점이 있다. 최근 들어 난포체외배양이 다양하게 개발되면서, 이러한 어려운 점을 극복할 수 있게 되었다. 본 논문은 각 발육단계의 난포를 절단해 체외배양하면서, apoptosis DNA 절단 현상을 이용하여 각종 난소국부 호르몬들이 난포발육단계별로 사망기전에 미치는 영향을 요약해 보였다. 난포는 발육하면서 점차 복잡한 호르몬 경로를 생존을 위해 필요로 한다. Prevulatory난포생존에 필요한 난소국부호르몬들은 early antral 단계의 난포에서는 그 미치는 영향이 감소되다가 preantral단계의 난포에서는 영향을 전혀 미치지 못했다. 단지 예외는 cGMP처리로써, 세포내 cGMP수준을 일정하게 유지시켜주는 것이 난포발육단계에 무관하게 생존에 중요한 인자로, 장래 연구는 난포 세포내의 cGMP수준을 조절하는 기작을 규명하는데 있을 것이다.인정되지 않았다. 7. 농지보전 처리구인 배수구와 초생수로구는 비처리구에 비해 낮은 침두 유출량과 낮은 토양유실량을 나타내었다.구보다 14% 절감되는 것으로 나타났다.작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도성 물질로 증착시키지 않고, Silver Paint 에 시편을 접착하는 방법으로도 만족한 시험 결과를 얻을 수 있었다.째, 회복기 중에 일어나는 입자들의 유입은 자기폭풍의 지속시간을 연장시키는 경향을 보이며 큰 자기폭풍일수록 현저했다. 주상에서 관측된 이러한 특성은 서브스톰 확장기 활동이 자기폭풍의 발달과 밀접한 관계가

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COMPARISON OF APICAL SEALING EFFICACIES USING DIFFERENT PLUGGING DEPTH IN CONTINUOUS WAVE OF OBTURATION TECHNIQUE (Continuous wave of obturation technique에서 플러거의 다양한 적용 깊이에 따른 근단부 폐쇄효율 비교)

  • Lee, Sang-Jin;Park, Jeong-Kil;Hur, Bock;Kim, Hyeon-Cheol
    • Restorative Dentistry and Endodontics
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    • v.32 no.6
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    • pp.491-497
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    • 2007
  • The purpose of this study was to compare apical sealing ability of continuous wave canal filling technique according to various heat source plugging depths. Eighty one extracted human premolars with straight root were cleaned and shaped to size 35 using .06 taper rotary NiTi file. After cleansing and shaping, the teeth were divided into 5 groups following the heat source probing depths from the apex; 3, 4, 5, 6 and 7 mm. All specimens were filled using E&Q plus with #35/.06 tapered gutta-percha cone. The positive control teeth were not filled. All teeth were coated with nail varnish except the apical 1 mm around the apical foramen. Negative control teeth were completely sealed include the apical foramen. All specimens were immersed in 1% methylene blue solution for 72 hours. Then the specimens were sectioned horizontally at 1, 2 and 3 mm from the root apex. Each sectioned surface was photographed using a digital camera attached to the stereomicroscope at $12.5{\times}2.5$ fold magnification. All points at 1, 2 and 3 mm were summed as final score of one specimen. Statistical analysis of the collected data was performed. Under the condition of this study. there was no significant difference between the heat source plugging depths of 3, 4, 5, 6 and 7 mm in apical sealing ability. All of apical heat source plugging depth from 3 to 7 mm including Buchanan's protocol -from 5 to 7 mm- seems to be acceptable in clinical application.

EFFECT OF PULSING ELECTROMAGNETIC FIELDS COMBINED WITH ANTERIOR MANDIBULAR DISPLACEMENT ON CONDYLAR GROWTH IN THE RAT (맥동 전자기장과 하악골 전방이동이 백서의 하악과두 성장에 미치는 영향에 관한 실험적 연구)

  • Yang, Sang-Duk;Suhr, Cheong-Hoon
    • The korean journal of orthodontics
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    • v.20 no.3 s.32
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    • pp.463-498
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    • 1990
  • 전기적 자극에 의한 골성장기전의 개념을 이용하여 임상적 효율성을 증진시키기 위한 연구는 현재 교정학을 비롯한 치과영역에서 활발히 진행되고 있는 분야 중의 하나이다. 전기적 자극의 여러 형태 중의 하나인 전자기장과 하악의 기능적 전방 이동을 유도하는 악기능교정장치가 백서의 하악과두 성장에 미치는 영향을 구명하기 위하여 본 연구를 시행하였다. 생후 4주된 Sprague Dawley계 백서 48마리를 대조군 12마리, 실험군 36마리로 나누고, 실험군은 다시 전자기장을 가한 군, 하악골 전방이동 장치를 장착시킨 군, 전자기장과 하악골 전방이동 장치를 병용시킨 군으로 분류하여 각각 12마리씩 실험동물을 배정하였다. 각 군의 실험동물은 15 HZ의 특수 전자기장이나 하악전방이동 자치가 하루10시간씩 작용되도록 특별히 제작한 실험장치 속에 넣어 1주간, 4주간씩 사육하여 희생시킨 후 하악골을 분리하고 연조직을 박리한 후 $10\%$ formalin에 보관하였다. 하악골 길이를 측정하기 위해 0.05mm까지 계측 가능한 캘리퍼를 이용하여 하악과두의 후연에서 이공까지의 거리를 계측하였고, 하악과두를 절제하여 0.5M EDTA에 탈회시켜 파라핀 포매를 하였다. 표본의 절단방향은 시상평면에 평행하게 하여 $6{\mu}m$두께로 연속절단 하였으며, 그 중 중심의 3절편을 취하여 통법에 의한 H-E 중염색을 시행하였다. 하악골 계측과 H-E 중염색 표본을 통한 조직학적 관찰을 통해 다음과 같은 결론을 얻었다. 1. 4주군에서 전자기장만에 노출된 실험군은 대조군에 비해 하악골 길이가 유익성 있게 증가되었다. 2. 전자기장과 하악골 전방이동 장치를 병용한 실험군은 하악골 전방이동장치만을 사용한 실험군에 비하여 하악골 길이가 증가되었다. 3. 전자기장에 노출된 실험군은 전구 연골아세포(prechondroblast)의 증식, 비대연골 세포층의 세포간질 및 연골내 골화층의 석회화가 모두 증가되었다. 4. 본 실험에 사용한 15 HZ전자기장의 주요작용부위는 백서의 하악과두 성장지역 중 연골내 골화의 석회화 지역이며, 또한 이는 하악골 전방이동 장치와 병용시 하악과두 성장을 촉진시킬 수 있음이 관찰되었다.

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PREVENTIVE EFFECT OF ADHESIVE TAPE SUPPLEMENTED WITH NaF ON ENAMEL EROSION IN VITRO (불소함유 접착테잎의 법랑질 침식 예방효과)

  • Lee, Sang-Ho;Lee, Nan-Young;Lee, In-Hwa
    • Journal of the korean academy of Pediatric Dentistry
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    • v.37 no.1
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    • pp.82-90
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    • 2010
  • This study examined the effect of adhesive tape supplemented with sodium fluoride on the prevention of dental erosion in vitro. Sound bovine tooth samples were selected and divided randomly into the following 4 groups according to the material treatments: group 1, APF gel; group 2, fluoride varnish; and groups 3 and 4, fluoride tape supplemented with 5% NaF in either a methyl cellulose or poly vinyl acetate carrier, respectively. All specimens were submitted to alternate cycles of acid exposure in a cola beverage (pH 4.3) and artificial saliva for $6\;{\times}\;5\;min/day$ over a 5 day period. The micro-hardness was recorded each day and the lesion depth was measured after 5 days. The micro-hardness of the experimental sides of groups 2, 3 and 4 were significantly higher than that of their control sides and the experimental side of group 1 during the experimental period (p<0.05) except on the 5th day. The enamel surfaces of treatment groups 2, 3 and 4 showed significantly higher resistance to mineral loss in terms of the erosion depth (p<0.05) than group 1 and their control sides. There was no statistically significant difference among group 2, 3 and 4, indicating that the fluoride varnish and tapes produce similar results. Fluoride adhesive tapes are effective in reducing the progression of erosion and can be recommended for young patients who are more susceptible to dental erosion.

Double Threshold Method for EMG-based Human-Computer Interface (근전도 기반 휴먼-컴퓨터 인터페이스를 위한 이중 문턱치 기법)

  • Lee Myungjoon;Moon Inhyuk;Mun Museong
    • Journal of Biomedical Engineering Research
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    • v.25 no.6
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    • pp.471-478
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    • 2004
  • Electromyogram (EMC) signal generated by voluntary contraction of muscles is often used in a rehabilitation devices such as an upper limb prosthesis because of its distinct output characteristics compared to other bio-signals. This paper proposes an EMG-based human-computer interface (HCI) for the control of the above-elbow prosthesis or the wheelchair. To control such rehabilitation devices, user generates four commands by combining voluntary contraction of two different muscles such as levator scapulae muscles and flexor-extensor carpi ulnaris muscles. The muscle contraction is detected by comparing the mean absolute value of the EMG signal with a preset threshold value. However. since the time difference in muscle firing can occur when the patient tries simultaneous co-contraction of two muscles, it is difficult to determine whether the patient's intention is co-contraction. Hence, the use of the comparison method using a single threshold value is not feasible for recognizing such co-contraction motion. Here, we propose a novel method using double threshold values composed of a primary threshold and an auxiliary threshold. Using the double threshold method, the co-contraction state is easily detected, and diverse interface commands can be used for the EMG-based HCI. The experimental results with real-time EMG processing showed that the double threshold method is feasible for the EMG-based HCI to control the myoelectric prosthetic hand and the powered wheelchair.

COMPARISON OF MARGINAL MICROLEAKAGE BETWEEN LOW AND HIGH FLOWABLE RESINS IN CLASS V CAVITY (5급 와동에서 저유동성과 고유동성 레진의 변연 미세누출 비교)

  • Bae, Sang-Bae;Cho, Young-Gon;Lee, Myeong-Seon
    • Restorative Dentistry and Endodontics
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    • v.34 no.6
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    • pp.477-483
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    • 2009
  • The purpose of this study was to compare the microleakage of low and high viscosity flowable resins in class V cavities applied with 1-step adhesives. Forty Class V cavities were prepared on the cervices of buccal and lingual surfaces of extracted molar teeth and divided into four groups (n=8). Cavities were restored with AQ Bond Plus /Metafil Flo $\alpha$, G-Bond/ UniFil LoFlo Plus (Low flow groups), AQ Bond Plus/Metafil Flo and G-Bond/UniFil Flow (High flow group), respectively. Specimens were immersed in a 2% methylene blue solution for 24 hours, and bisected longitudinally. They were observed microleakages at the enamel and dentinal margins. In conclusion, the low viscosity flowable resins showed lower marginal microleakage than do the high vis cosity flowable resins in class V cavities.

Statistical analysis of estimating incubation period distribution and case fatality rate of COVID-19 (COVID-19 바이러스 잠복 시간 분포 추정과 치사율 추정을 위한 생존 분석의 적용)

  • Ki, Han Jeong;Kim, Jieun;Kim, Sohee;Park, Juwon;Lee, Joohaeng;Kim, Yang-Jin
    • The Korean Journal of Applied Statistics
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    • v.33 no.6
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    • pp.777-789
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    • 2020
  • COVID-19 has been rapidly spread world wide since late December 2019. In this paper, our interest is to estimate distribution of incubation time defined as period between infection of virus and the onset. Due to the limit of accessibility and asymptomatic feature of COVID-19 virus, the exact infection and onset time are not always observable. For estimation of incubation time, interval censoring technique is implemented. Furthermore, a competing risk model is applied to estimate the case fatality and cure fraction. Based on the result, the mean incubation time is about 5.4 days and the fatality rate is higher for older and male patient and the cure rate is higher at younger,female and asymptomatic patient.

Study on Optimization of Persimmon Kochujang Jangachi Using Response Surface Methodology (반응표면분석법을 이용한 단감 고추장 장아찌 품질의 최적화 연구)

  • Sim, Hye Hyeon;Choi, Ok-Ja
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.9
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    • pp.1364-1373
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    • 2015
  • The purpose of this study was to determine the optimum sodium concentration (0~8%), soaking time (4~20 min) and storage time (0~60 day) for preparation of persimmon jangachi with kochujang sauce using response surface methodology. Physicochemical properties (salinity, pH, Hunter's color value, cutting force, and sensory evaluation) of persimmon kochujang jangachi were analyzed during storage at $20^{\circ}C$ for 60 days. When the proximate composition of persimmon was analyzed, moisture content, crude protein content, crude lipid content, and crude ash content were 85.41%, 0.51%, 0.22%, and 0.20%, respectively. For persimmon kochujang jangachi manufactured with different sodium concentrations, soaking times, and storage times, salinity, pH, Hunter's color value of L, a, and b, color, flavor, taste, texture, and overall preference were represented by a quadratic model. Cutting force was represented by a linear model pattern. In conclusion, the optimal formulation for persimmon kochujang jangachi, as assessed by numerical and graphical optimization methods, was a sodium concentration of 6.91%, soaking time of 11.36 minutes, and storage time of 25.18 days.

Making Techniques of Hight Quality Powder in Sweetpotato (고구마 고품질 분말 제조기술)

  • Lee, Joon-Seol;Ahn, Young-Sup;Kim, Hag-Sin;Chung, Mi-Nam;Jeong, Byeong-Choon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.spc1
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    • pp.198-203
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    • 2006
  • This study was conducted to select the temperature for hot air drying method, cutting method, and solution that inhibits enzymatic browning in making high quality powder from sweetpotato roots. It was observed that $70^{\circ}C$ was the best temperature for air drying method wherein the dry matter rating, dry matter time, flour yield and chemical component were considered. The proper cutting thickness should be 2.0 mm considering the vitamin C content and sensory evaluation of sweetpotato flour. Also, 1% vitamin C solution inhibited enzymatic browning of sweetpotato.