• Bioinformatics and Biosystems
• /
• v.1 no.2
• /
• pp.99-102
• /
• 2006

The Basic Local Alignment Search Tool (BLAST) is one of the most established software in bioinformatics research and it compares a query sequence against the libraries of known sequences in order to investigate sequence similarity. Expressed Sequence Tags (ESTs) are single-pass sequence reads from mRNA (or cDNA) and represent the expression for a given cDNA library and the snapshot of genes expressed in a given tissue and/or at a given developmental stage. Therefore, ESTs can be very valuable information for functional genomics and bioinformatics researches. Although major bio database (DB) websites including NCBI are providing BLAST services and EST data, local DB and search system is demanding for better performance and security issue. Here we present animal EST DBs and local BLAST search system. The animal ESTs DB in NCBI Genbank were divided by animal species using the Perl script we developed. and we also built the new extended DB search systems fur the new data (Local Animal BLAST Search System: http://bioinfo.kohost.net), which was constructed on the high-capacity PC Cluster system fur the best performance. The new local DB contains 650,046 sequences for Bos taurus(cattle), 368,120 sequences for Sus scrofa (pig), 693,005 sequences for Gallus gallus (fowl), respectively.

• Journal of Animal Science and Technology
• /
• v.48 no.1
• /
• pp.1-8
• /
• 2006

KIT encodes a mast/stem cell growth factor receptor and is known as a possible candidate gene responsible for dominant white coat color in mammals. To investigate the genetic variation of KIT gene in Korean wild boars(Sus scrofa coreanus), we carried out PCR-RFLP and DNA sequencing for three exons(exons 17, 19, and 20) and intron 19 of the KIT gene in Korean wild boars. PCR-RFLP results using NlaⅢ restriction enzyme in the breakpoint region between exon 17 and intron 17 and AciⅠ restriction enzyme in exon 19 indicate that Korean wild boars did not have previously identified white coat color related splicing mutation and missense mutation, respectively. These results also indicate matings between Korean wild boars could not give white coat color offsprings. We also found new SNPs in exons 19(C2661T) and 20(A2760G). Of these, the SNP in exon 20 is a missense mutation which might induce the change of amino acid iso-leucine to valine. However, no relationship was identified with this missense mutation and coat color. In this study, breed specific new SNPs were identified in exons 19, 20 and intron 19 and these results will give important information for genetic variation of porcine KIT gene.

• Journal of dental hygiene science
• /
• v.11 no.4
• /
• pp.325-332
• /
• 2011

To get the evidences for decreasing the discomfort, dissatisfaction, and for improving management of the implant prothesis through assessment implant patients' awareness and discomforts about care of the prostheses. The subjects were 300 implant patients who treated in the 20 dental clinics in Pusan, Ulsan and Daegu. The general characteristics, recognition, maintenance, discomfort and dissatisfaction, and oral hygiene status were collected. Level of the discomfort and dissatisfaction was measured by a 5 Likert scale, and analyzed by the equivalent of 100. The life-span of the implants was overestimated, however, periodic recall check and scaling were underwent. The level of discomfort and dissatisfaction was generally good, but many of them (65.3%) complained the food impaction. As a result of the multiple regression analysis, level of the discomfort and dissatisfaction increased in case of more number of the prosthesis ($\beta$=0.864), however decreased in case of more frequent teeth brushing ($\beta$=0.737), more frequent periodic check ($\beta$=-0.737), longer duration from the implantation ($\beta$=-0.090). It is very important for the implant patients to be recognized that the lack of care may shorten the life-span of the implant prosthesis. Therefore, efforts of the continuous oral hygiene care such as, periodic recall check, scaling, teeth brushing, the correct using of additional oral hygiene product, and education would be more emphasized.

• Korean Journal of Veterinary Service
• /
• v.43 no.3
• /
• pp.139-145
• /
• 2020

This study was carried out to identify the occurrence of transovarian transmitted diseases and antibody positive rates among Korean native breeder chickens. The infection rates with Salmonella pullorum and Salmonella gallinarum among 16-week-old, 36-week-old, and 56-week-old breeder chickens and the antibody positive rates to Egg Drop Syndrome '76, Mycoplasma gallisepticum and Mycoplasma synoviae among 16-week-old, 18-week-old, and 56-week-old breeder chickens were identified, and the antibody positive rates to seven major transovarian transmitted diseases among 1-day-old chicks were investigated. As a result, no infection with Salmonella pullorum and Salmonella gallinarum was found among the investigated subjects of all ages. Vaccinated breeder chickens showed the 100% antibody positive rate to Egg Drop Syndrome '76, and unvaccinated breeder chickens showed the 100% antibody negative rate to Mycoplasma gallisepticum and Mycoplasma synoviae, confirming that there was no infection with Mycoplasma gallisepticum and Mycoplasma synoviae. As a result of the antibody tests of the 1-day-old chicks for transovarian transmitted diseases, it was found that vaccinated chicks showed good antibody positive rates to avian encephalomyelitis, chicken infectious anemia, and avian reovirus, confirming that they had power of defense against the relevant infectious diseases, and that unvaccinated chicks showed the 100% antibody negative rates to avian leukosis, chicken reticuloendotheliosis, and Mycoplasma synoviae, confirming that there was no infection with the relevant diseases. Given that the results of this study showed that among the transovarian transmitted diseases of chickens, there was no history of infection with diseases against which vaccination was not administered and high antibody positive rates were found with diseases against which vaccination was administered, it is judged that chickens with good power of defense against diseases were bred, and it is deemed that constant monitoring and vaccination against transovarian transmitted diseases will be necessary for the control and prevention of the diseases.

• The korean journal of orthodontics
• /
• v.36 no.1 s.114
• /
• pp.63-73
• /
• 2006

Numbers of postulations lie on the difference of integumental changes with two major surgical remedies of one jaw vs. two jaw surgery in skeletal Class III malocclusion. Accordingly it was the aim of the study to elucidate the skeletal profile changes with an accompanying disposition of soft tissues, consequently to yield the correlation and ratio of soft tissue changes with two types of surgical procedures, which in turn make it possible to predict the soft tissue outcomes by means of assembled regression equations. Cephalometric headfilms of fifty two adult skeletal Class III comprised of 26 maxillary advancement by Le Fort I osteotomy and mandibular setback by sagittal split ramus osteotomy simultaneously (double jaw surgery, group A), 26 mandibular setback alone (one jaw surgery, group B) were statistically analyzed. Group A manifested 72.4% soft tissue advancement to skeletal changes in the upper lip area, while group B appeared to have no statistically significant changes. The nasolabial angle showed more increment in group A than in group B, whereas the mentolabial angle illustrated more reduction in group B. The backward movement of soft tissue pogonion to skeletal change revealed 98% in group A, and 109% in group B. The double jaw surgery group characteristically revealed remarkable integ umental change in the upper lip area, while the one jaw surgery had major effects in the lower lip and soft tissue pogonion areas.

• Korean Journal of Environmental Biology
• /
• v.35 no.3
• /
• pp.380-388
• /
• 2017

Factors affecting spatial distribution of halophytes were analyzed in June 2012 at the Goraebul coastal dunes. In the Goraebul sand dune, distribution of halophytes was divided into three groups. The first group belonging to Elymus mollis, Carex kobomugi, Calystegia soldanella, Ixeris repens and Glehnia littoralis was distributed in the ridge of primary sand dune and dune slack. The second group belonging to Lathyrus japonicus and Zoysia macrostachya was distributed in the dune slack. The third group belonging to Pinus thunbergii, Vitex rotundifolia and Linaria japonicus was distributed in the pine forest of the secondary sand dune. E. mollis, C. kobomugi, C. soldanella, I. repens and G. littoralis was distributed in relatively unstable habitat of sand dunes due to the large amount of sand movement. V. rotundifolia was distributed in a relatively stable habitat. Factors that have the greatest influence on distribution of halophytes in the Goraebul sand dunes are distance from the seashore, topography, and the pine forest. The Goraebul sand dune is a relatively well-preserved area with minimal human intervention. Therefore, different distribution of physico-chemical factors by natural processes is essential to spatial distribution of halophytes than other sand dunes in Korea. Significant natural processes in the Goraebul sand dunes were advance and retreat of coastlines from waves, erosion and sedimentation of sand due to wind and waves, and dispersal of seawater.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.25 no.2
• /
• pp.70-74
• /
• 1989

This paper, described on the maneuverabilities of M.S. Cheju 402, the stern trawler (159 G.T.), training ship of Cheju National University which carried out the turning circle tests at helm angle 35$^{\circ}$ and 10$^{\circ}$, 20$^{\circ}$, 30$^{\circ}$Ztests of her. The results obtained were as follows; 1. The advances of the starboard and port turning circles were 79.1m, about 2.6 times of the length of the ship, and 81.4m, about 2.7 times of it, respectively. 2. The rates of speed reduction were about 0.49 together, and mean values of turning angular velocity of her at helm angle 35$^{\circ}$ into the starboard and port sides were 4.3$^{\circ}$/sec and 4$^{\circ}$/sec during the turning movement. 3. Overshoot angles of starboard side werelarger than those of port side at all Z tests, and mean values of them of the starboard and port sides at 10$^{\circ}$, 20$^{\circ}$, 30$^{\circ}$Z tests were 110.5$^{\circ}$ 20.5$^{\circ}$, 28.5$^{\circ}$ respectively. 4. The maneuvering indices K and T of experimental ship at 10$^{\circ}$, 20$^{\circ}$, 30$^{\circ}$, Z tests were 0.755 and 3.468, 0.566, and 1.621, and 0.481 and 1.547 respectively. Consequently, the experimental ship showed that her turning ability was more in effective as the helm angle was becomed larger and her obeying ability was more effective as it was becomed larger.

• Development and Reproduction
• /
• v.15 no.4
• /
• pp.385-391
• /
• 2011

Human in vitro fertilization and embryo transfer (IVF-ET) program is a general procedure for infertile couples since first successful delivery on 1978 in UK and Korean first on 1985. Recently in Korea, more than 42,000 cases per year of IVF-ET were performed and showed good pregnancy rates compared worldwide data. The human IVF-ET procedure use many consumables, such as ovum pick-up (OPU) needles, centrifuge tubes, culture dish, ICSI pipette, culture media and ET catheters. Major of these materials are supported by the global companies. Thus, Korean IVF-ET program might be placed unstable situation by global economical risks. These uncertain problems could be overcome by the domestic production of IVF-ET materials and consumables. Two times questionnaires for Korean clinicians and researchers about the domestic production were performed and analyzed. Many of them requested domestic OPU needles, ET catheters, culture media and ICSI pipettes under good quality control and quality assurance system. This trial may be contributed to industrialization and to global competence of Korean IVF-ET program. The results of this survey can be provide a fundamental base for development and production of domestic materials and consumables for human IVF-ET program.

• Korean Journal of Biological Psychiatry
• /
• v.2 no.1
• /
• pp.100-106
• /
• 1995

Antibodies to hepatitis C drew attention because of high morbidity to chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. HCV was known to be transmitted by transfusion, sexual behavior and parenteral drug use. However, some kind of autoimmune mechanism was suggested to be involved in the genesis of HCV-induced liver diseases. We hypothesized the prevalence of having anti-HCV might be higher in psychiatric patients rather than general population because of the characteristic route of transmission. Using Abbott HCV BA kit, anti-HCV was detected in the sera of 113 psychiatric inpatients from early December in 1992 to late May in 1994. The Positivity of anti-HCY was significantly(P<0.05) higher among psychiatric inpatients(10.6%) than in healthy controls(3.0%). There were no disease specificity among psychiatric inpatients who had anti-HCV, though alcoholics tended to have more anti-HCV. We couldn't find any significant correlation of anti-HCV with age, seasons of birth, lymphocytes (%) and liver function.

• Journal of Information Management
• /
• v.41 no.3
• /
• pp.105-130
• /
• 2010

Science and technology information resources are major impetus for all research and development activities. Now countries around the world are seeking efficient and effective means to cope with the exponential growth of needs for science and technology information for research. In this context, it has been perceived vital to establish national infrastructures in order to extend the availability of world's science and technology information. In Korea, it has been a recurring issue to build a national science library to prevent knowledge colonization and to gain a competitive edge in the fields. It is essential to have one as a national information infrastructure to assume the role of developing a sustainable model for access to the world's science and technology information, providing permanent access to them. In this paper, therefore, some issues concerning building and managing a new national science library in Korea are examined. In doing so, the following details are dealt with in detail. First, the rationale behind building a national science library is described, Second, the functions and roles of the library are defined, Third, previous discussions and attempts to build a national science library are reviewed and analysed. Finally, possible problems and issues raised in relation to build a national science library are probed, and future directions are also suggested. In this process, the recent strategic plan for national science library, national agriculture library, and national medical library, and changing plan for Sejong city are taken into consideration as influencing factors.