• Title/Summary/Keyword: 전기특성

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Characteristics of Light-evoked Retinal Ganglion Cell Activity with Postnatal Maturation in SD Rat (SD rat 망막신경절세포의 생후 성숙기간에 따른 빛 자극 반응 특성)

  • Ye, Jang-Hee;Goo, Yong-Sook
    • Progress in Medical Physics
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    • v.16 no.4
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    • pp.214-219
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    • 2005
  • As part of Korean retinal prosthesis project, we have provided preliminary experimental results regarding voltage parameters for the stimulation of chemically degenerated rabbit retina. Since our APB-treated chemically degenerated retina is only ON-pathway blocked, now we switch our experiments to more appropriate retinal degeneration model, genetically degenerated retina model (RD mouse: rd/rd (C3H/HeJ)). Before studying with RD mouse, we started control experiments with normal SD rat to understand characteristics of retinal ganglion ceil activity with postnatal maturation in rodents. Ganglion cell activities were recorded with 8${\times}$8 multi-electrode array. Moving spontaneous bursts appeared until postnatal day of 15. During pre-eye opening period, no light evoked response appeared. After postnatal day of 2 weeks (post-eye opening period), ON-, OFF- and ON/OFF response appeared. The fractional distributions of ON, OFF, and ON/OFF ganglion cell is about $40\%,\;50\%$, and $5\%$. The percentage ($\%$) of light evoked response in each dorso-temporal, ventral, and dorso-nasal area of eye is about $50\%,\;37.5\%$ and $12.5\%$, respectively. We concluded that the optimal period for experiment in rodent is about postnatal day of 2${\~}$3 weeks.

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Clinical Characteristics of Neonatal Status Epilepticus (신생아 간질 중첩증의 임상 특성)

  • Jung, Kyeong Hun;Kim, Yun Hee;Kwon, Young Se;Jun, Yong Hoon;Kim, Soon Ki;Son, Byong Kwan
    • Clinical and Experimental Pediatrics
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    • v.48 no.12
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    • pp.1342-1347
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    • 2005
  • Purpose : Among perinatal risk factors, neonatal seizures are one of the strongest independent discriminators of adverse outcome, representing high risks of mortality and neurologic morbidity. This study was undertaken to evaluate the neurologic outcome of neonatal status epilepticus according to underlying etiology, seizure pattern, onset time, and duration. Methods : We reviewed retrospectively 36 neonates(19 males, 17 females) with status epilepticus who were admitted to the neonatal intensive care unit, Inha Hospital between July, 1988 and June, 2003. They were evaluated with neurologic examination, laboratory data, EEG findings, and neuroimaging studies etc. Results : The mean gestational period of the patients was $37.0{\pm}3.6$ weeks and birth weight was $2.70{\pm}0.82$ kilogram. Fifty two point eight percent of the neonates were male and 66.7 percent were born at term. The most common cause of neonatal status epilepticus was hypoxic-ischemic encephalopathy. In preterm babies, intracranial hemorrhages showed an especially high frequency(P=0.034). Gestational age and birth weight did not show a correlation with neurologic complications. The incidence of neurological sequelae were significantly related to prolonged seizures lasting more than 1 hour(P=0.002). Neonates with seizures within the first 72 hours tended to be more frequent among those who developed adverse outcomes(P=0.016). Generalized tonic seizures had the worst prognosis, whereas those children who had subtle seizures had better outcomes than any other type(P<0.05). Generalized tonic seizures were primarily represented on EEG by abnormal background, whereas subtle seizure showed a significantly more normal EEG than any other seizures(P<0.05). Conclusion : Our results indicate that neonatal status epilepticus with early onsets, prolonged durations. And generalized tonic types can predict an increased risk for neurologic sequelae. So, those seizures must be perceived as medical emergencies and treated aggressively with antiepileptic drugs.

A study on the narrative use of transitional object-characters in the family feature animation (가족용 장편애니메이션<인사이드 아웃>에 나타난 이행대상(transitional object) 캐릭터의 서사적 활용 연구)

  • Park, Hyoung-Dong
    • Cartoon and Animation Studies
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    • s.49
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    • pp.325-357
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    • 2017
  • It can be assumed that the reasons why the animation characters, 'Olaf (in Frozen Kingdom)' and 'Minions (in Super Bad)', etc., which were very successful in the merchandising market while having won the popularity better than the main characters are very popular even though such characters appeared only by playing a funny role while assisting the adventures of the main characters are not only because of their cute appearances but also because such characters have their own core features in their inner world as the transitional object-characters. Simply expressing, a 'Transitional Object' as a concept suggested by a child psychologist, 'Donald Winnicott', means a lovey doll or an imaginary friend which temporarily replaces an infant's mother during the procedure when the infant is mentally separated from its mother. However, in case that the theory of transitional objects was applied directly to many narrative content characters for doing a study, there must have been done some studies in advance for establishing some new criteria and indexes related to the transitional object-characters of such narrative contents. Accordingly, while thinking that the 'emotional relationship' between a growth-subject and a growth mediator must be dealt with as the most important content in order to define a transitional object-character in a narration clearly, this researcher established some emotional index for judging the propensities of a transitional object-character on the basis of such way of thinking. The index is composed of 4 kinds of emotional roles (quasi-family member, growth mediator, lovey doll, an imaginary friend), 6 kinds of emotional supports (hugging, protecting, accepting, giving the initiative, improving the relationship and mutual supervising) and 4 kinds of emotional impressions (impression by contacting, impression to protect and impression accepting an attack). In case that some main characters of a family feature animation, 'Inside Out', are analyzed while the index mentioned above is applied, it was found that 'Bing Bong' and 'Sadness' have a high propensity as a transitional object-character. Especially, it could be inversely inferred in which ways some good transitional object-characters can help the narrations on growth of a family feature animation by taking a look at the character, 'Sadness' that has the highest propensity as a transitional object-character. The transitional object-character, 'Sadness' assists the narration on growth internally and externally by helping the internal maturity of a growth-subject in a way of projecting the tasks for the internal maturity of a growth-subject while helping the growth-subject to be successfully externally in a way of providing the growth-subject with some kinds of facilitating emotion. As the results from this Study, since such kinds of emotional experiences provided by such transitional object-characters are displaced to not only the relevant growth-subjects but also the audience who are emphasized with such growth-subjects as they are, such transitional object-characters play a role of hidden benefactors who induce some immersion into the narrations and provide child and adult audience with various layers of emotional satisfaction.

The Effect of Alcohol Fermented Feedstuff Made of Byproducts on In vitro Fermentation Characteristics and NDF Disappearance in the Rumen (조사료 대용 산업부산물을 이용한 알코올 발효사료가 In vitro 반추위내 발효특성 및 NDF 분해율에 미치는 영향)

  • Kim, Byong-Wan;Lin, Guang-Zhe;Park, Byung-Ki;Kim, Jong-Duk;Shin, Jong-Suh
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.27 no.4
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    • pp.323-334
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    • 2007
  • An in vitro study was conducted to examine the influence of alcohol-fermented feedstuff formulated with byproducts on the fermentation characteristics and NDF disappearance in the rumen. Dietary treatments were either a soybean curd-based alcohol-fermented feedstuff (AFS) or brewery grain-based alcohol-fermented feedstuff (AFB). The AFS and AFB are composed of 50% commercial beef cattle feed, 50% soybean curd dreg, 5% molasses and 0.5% yeast and 25% commercial beef cattle feed, 25% brewery grain, 25% soybean curd dreg, 25% corn grit, 5% molasses and 0.5% yeast, respectively. The change of ammonia, pH alcohol, volatile fatty acids, and NDF disappearance were measured at 0, 2, 4, 8 and 12 hr after in vitro incubation in the rumen. After 2 hr incubation, higher ammonia concentrations were resulted in AFS (12.47 mg/dl) and AFB (12.85 mg/dl) compared to control (11.84 mg/dl) (p<0.05). Ruminal pH of AFS and AFB were significantly higher than control during 1 to 6 hr fermentation, but the pH of AFS and AFB were decreased after 6 hr. At 12 hr fermentation, the alcohol concentration of AFS and AFB was significantly increased by 43.9% and 48.0%, respectively. The acetate concentration was rapidly decreased in control, while the concentration was slowly decreased in AFS and AFB with increasing the fermentation time. Lower concentrations propionate and butyrate were observed in AFS and AFB during every fermentation time (p<0.05). The NDF disappearance was significantly lower in AFS and AFB after 4 hr fermentation. These results suggest that alcohol fermented feedstuff can control the fermentation pattern in the rumen.

Optimum Nutrient Solution Strength for Korean Strawberry Cultivar 'Daewang' during Seedling Period (국내 육성 신품종 딸기 '대왕'의 육묘기 적정 배양액 농도)

  • Jun, Ha Joon;Jeon, Eui Hwan;Kang, Soo In;Bae, Geun Hye
    • Horticultural Science & Technology
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    • v.32 no.6
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    • pp.812-818
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    • 2014
  • Raising seedlings is important for fruit crops and is especially significant for strawberries as it accounts for 80% of their cultivation. However, there are few studies on raising seedlings of strawberries by hydroponics. Since strawberries show clear differences in growth characteristics based on cultivar, it is necessary to develop suitable fertilizer formula, concentration and pH for each cultivar, and also to examine the amount of nutrient feeding appropriate for each medium type. A key to raising seedlings of strawberries by hydroponics is the development of strategies to manage the concentration of nutrient solution. The mother plants of 'Daewang' strawberries were planted on hydroponics benches filled with cocopeat on March 28, 2012. Three nutrient solution treatments were employed during the term of raising seedlings: a type that supplied EC $0.6dS{\cdot}m^{-1}$ nutrient solution for 30 days and only water for 20 days [0.6 (30) + 20]; a type that supplied EC $1.2dS{\cdot}m^{-1}$ nutrient solution for 30 days and only water for 20 days [1.2 (30) + 20]; and a type that supplied EC $1.2dS{\cdot}m^{-1}$ nutrient solution for 50 days [1.2 (50)]. The plants were then planted on hydroponics benches filled with cocopeat on September 20, and managed with EC $0.6-0.8dS{\cdot}m^{-1}$ strawberry nutrient solution developed by Yamazaki. After planting, shoot growth, flowering rate and fruit quality of the first cluster were investigated. The petiole length, leaf length, leaf width and crown diameter showed the highest grown in the [1.2 (50)] treatment, followed by [1.2 (30) + 20], and then [0.6 (30) + 20], indicating that the higher concentration of nutrient solution was preferable for raising seedlings. However, the growth differences among treatments gradually disappeared as growth continued, and the crown diameter especially grew to exhibit almost no difference at all among treatments. The point of flowering came first in [0.6 (30) + 20], followed by [1.2 (30) + 20] and then [1.2 (50)]. The [0.6 (30) + 20] treatment showed much earlier flowering than other treatments, which implies that low-concentration nutrient solution may be beneficial to the flowering of 'Daewang' strawberries while raising seedlings. There was no statistically significant difference among treatments in fruit length, fruit diameter and fruit firmness. Fruit weight in [1.2 (50)] treatment was significantly higher than other treatments. However, soluble solids of fruit was the lowest in [1.2 (50)] treatment. Together, the results of this experiment imply that it is better to supply EC $0.6dS{\cdot}m^{-1}$ solution for 30 days and then supply only water for 20 days to adequately manage concentration of nutrient solutions during the period of raising seedlings of strawberries by hydroponics.

Studies on Prevalence of Meat Allergy and Potential Beef Allergens (식육알레르기 발생 경향 및 잠재적 우육알레르겐에 관한 연구)

  • Jeong, Bo-Young;Kim, Dong-Yeop;Fan, Jiang Ping;Chung, Hyun-Chae;Han, Gi-Dong
    • Food Science of Animal Resources
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    • v.29 no.2
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    • pp.151-156
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    • 2009
  • The prevalence of food allergies was investigated using questionnaires with 300 subjects whose ages ranged from 19 to 24 years old and the causative food allergens was analyzed using immunological analysis with serum of the subjects who answered that they have/had food allergy. The questionnaire showed that 11.33% of subjects have/had experience of food hypersensitivity, where the main causative foods were fish, beef, chicken, milk, egg, and pork in order. The meat allergy shared 4.65% (2.33% for beef, 1.66% for chicken, 0.66% for pork) in the prevalence of food allergies. The causative beef allergens were investigated with the serum of 6 subjects who have had beef allergy. Western blots were carried out with the serum of P6 subject who showed a positive reaction to beef extract in ELISA. The two specific bands were detected in beef extract on the PVDF membrane, and no band was detected in extracts of pork and chicken. A calculation of the distance of migration by SDS-PAGE enabled the molecular masses of the two bands to be estimated as 67kDa and 31kDa, respectively. The 67kDa was revealed as bovine serum albumin (BSA) which is one of the important beef allergens as reported previously though an analysis of the N-terminal amino acid sequence. However we could not identify the sequence of 31kDa, probably because they comprised several subunits and were modified proteins such as glycoprotein that were unlikely to be easily degraded by the Edman method. The 31kDa band were dyed with the PAS (periodic acid-schiff reagent), suggesting that it might be a glycoprotein. These results suggested that the 31kDa might be considered as a novel potential beef allergen which is not reported previously, although further studies are needed.

Effects of Soil Bulk Density on Saturated Hydraulic Conductivity and Solute Elution Patterns (토양의 용적밀도에 따른 포화수리전도도 및 음이온의 용출양상)

  • Kim, Pil-Joo;Lee, Do-Kyoung;Chung, Doug-Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.30 no.3
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    • pp.234-241
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    • 1997
  • The effects of bulk densities(${\rho}_b$) on saturated hydraulic conductivity (Ksat) and solute elution patterns were investigated from five different bulk densities ranging from $1.1Mg/m^3$ to $1.5Mg/m^3$ with each increment of $0.1Mg/m^3$. The hydraulic conductivities observed were divided into two stages: (1) a linearly decrease with increase in bulk density up to $1.4Mg/m^3$, (2) a steady state where the bulk density is greater than $1.4Mg/m^3$. Using the saturated hydraulic conductivity at the steady state, we figured out the equation describing the correlation between bulk densities(${\rho}_b$) and saturated hydraulic conductivity(Ksat) as follows: $Ksat=-19.2({\rho}_b{^2})+6{\rho}_b+15.5$, (r=0.985). Electrical conductivity(EC) measured from the leachate of the soil column showed that EC at the same pore volume were decreased with an increase in the bulk density from $1.2g/cm^3$, $1.5g/cm^3$, as shown in the time taken to collect the same pore volume at each respective bulk density. The maximum relative concentrations (C/Co=1) from the breakthrough curves for the anions of $Cl^-$, $NO_3{^-}$ and $SO_4{^{2-}}$, which are weakly adsorbed on the soil particles, moved to the right of the graph, while a distinctive retardation occurs at the bulk density between $1.3Mg/m^3$ and $1.4Mg/m^3$. The time taken to recover about 90% of indigenous sulphate was approximately twice as those of chloride and nitrate, resulting in slightly stronger adsorption characteristics for sorption sites on the soil surface. Thus, we can conclude that the salt accumulation in green house soil might be significantly influenced by it's bulk density at the soil depth, as well as the adsorption capacity of ions for the sorption sites in soils.

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Combinations of Paraquat and Oxyfluorfen for Control of Orchard Weeds (과수원(果樹園) 제초(除草)를 위한 Paraquat 와 Oxyfluorfen 조합처리(組合處理) 효과(效果)에 관한 연구(硏究))

  • Kwon, S.L.;Guh, J.O.;Cho, Y.W.
    • Korean Journal of Weed Science
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    • v.3 no.2
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    • pp.208-222
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    • 1983
  • To control ideally the weeds in orchards and systematize the utility of herbicides, the mixing effect of oxyfluorfen and paraquat was evaluated. Combinations of oxyfluorfen with paraquat were increased the control efficacies at any treatment without giving damage to fruit trees and appeared a synergism. The control effects were reduced from 80-90% to 40-60% as the time advanced. However, the relative synergistic index was higher at 60 days after treatment in preemergence treatment and at 90 days after treatment in early and late postemergence treatment, respectively. Also the index was relatively higher at lower dosage of both herbicides.

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Origin of Sandstone Fragments Within Core Sediments Obtained from Southwestern Continental Shelf of the Ulleung Basin, East Sea (동해 울릉분지 남서부 대륙붕에서 채취된 시추퇴적물내 사암편의 기원)

  • Lee, Eui-Hyeong;Lee, Yong-Kuk;Shin, Dong-Hyeok;Huh, Sik;Kim, Seong-Ryul;Jeong, Baek-Hoon;Han, Sang-Joon;Chun, Jong-Hwa
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.6 no.3
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    • pp.126-134
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    • 2001
  • Several angular sandstone fragments (about 7 cm in longest diameter) occur in two piston cores, obtained from the submarine trough in the northeastern part of Korea Strait. The origin of the sandstone fragments and the paleoenvironment of trough sediment could be suggested from sedimentary facies analysis of cores and identification of ostracod within sandstone fragments. Echo characteristics around two core sites in submarine trough represent the prolonged bottom echoes with diffuse or no subbottom reflectors. The cores consist of a lower bioturbated mud and an upper gravelly sand sediments with sandstone/shell fragments. The bioturbated mud sediments show low water contents (27-44%) and high shear strength (19.2->37 kPa) compared with those of Holocene sediments (60-219% and 1.0-2.7 kPa, respectively) in the inner shelf and continental slope. However, clay contents (48-56%) of the bioturbated mud sediments are similar to those of fluviatile Holocene sediments in the inner shelf. The mean grain size of gravelly sand sediments ranges from 2.3 to 3.0 ${\phi}$ and shows coarsening upward with sandstone/shell fragments. The Holocene palimpsest in the continental shelf are composed of muddy sand sediments or sandy mud sediments (mean grain size: 4.6-7.6 ${\phi}$). Those suggest that two core sediments might be formed from Paleofluvial and paleocoastal deposits during sea-level lowstand. However, sandstone fragments mainly consist of quartz grains and bioclasts, with carbonate matrix, hollow pore, and glauconite. Two extinct ostracod species, Normanicythere sp. and Kotoracythere sp., are recovered in the sand-stone fragments of core EP-7, and they continued to exist from late Pliocene to early Pleistocene in cold water environment of this area. Thus, the sandstone fragments are interpreted to be formed at the paleocoastal environment derived from the Plio-Pleistocene outcrops exposed around the submarine trough during the LGM (Last Glacial Maximum) period.

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Zooplankton Abundance in Korean Waters (한국근해 동물성 부유생물의 주요군의 양적 분포)

  • Park, Joo-suck
    • 한국해양학회지
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    • v.8 no.1
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    • pp.33-45
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    • 1973
  • Plankton samples used for the present study were collected by the NORPAC net during the CSK cruises in the Korean waters in March and August, 1967. Regional and seasonal variations in the zooplankton biomass (wet weight, mg/㎥) were noticed in the Korean waters. In March the highest biomass, 130mg/㎥ on the average, occurred in the southern part of Japan Sea, but the lowest biomass of less than 50mg/㎥ occurred in the Yellow Sea and the western sea of Cheju Island Contrally, in August, the average biomass of 120mg/㎥ was measured in the Yellow Sea, the western sea of Cheju Island and the coastal waters of southern Korea, while the biomass of Japan Sea was the lowest of the regions surveyed. In comparison with the zooplankton biomass, total number of zooplankton per cubic meter of water strained also showed regional and seasonal fluctuations. In general, variations in the number of zooplankton specimens follows the same trend as in the biomass. The largest number, up to 800mg/㎥ on the average, occurred in the southern part of Japan Sea in March and the lowest number, less than 200mg/㎥ occurred in the Yellow Sea and the western sea of Cheju Island. In August, as shown by the biomass fluctuations, the largest number of zooplankton 850mg/㎥ on the average occurred in the Yellow Sea, the western sea of Cheju Island and the coastal region of southern Korea. But the lowest number of less than 500mg/㎥ was found in the Japan Sea. Among the various groups of zooplankton examined, the following were dominant components of the zooplankton population: Copepoda, Chaetognatha, Siphonophora, Euphausiacea, Cladocera, Appendicularia, and Amphipoda. The zooplankton conposition was significantly differed between the Japan Sea and Yellow Sea. Copepods which usually occupied over 66% in the Japan Sea and thd Korean Strait samples occupied only 42% of the catches in August, while cladocerans and chaetognaths were relatively abundant, i. e., 15 and 18% of the total organisms. The most dominant species of copepods and chaetognaths were Paracalanus parvus, Oithona similis, Acartia clausi, Calanus helgolandicus, Sagitta enflata, S. bedoti, S. elegans and S. crassa.

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